不同品种杏果实发育过程中类黄酮、总酚和三萜酸含量及抗氧化性研究
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摘要
【目的】探讨不同品种杏果实发育过程中类黄酮、总酚、三萜酸含量的差异及与抗氧化活性的关系。【方法】以‘金太阳’‘凯特’‘华县大接杏’‘沙金红’‘金香’‘早金艳’等6个杏品种为试材,对不同品种杏果实发育过程中类黄酮、总酚、三萜酸的含量及清除羟基自由基(·OH)、DPPH自由基(DPPH·)和超氧阴离子自由基(·O2-)的能力进行分析测定。【结果】1.供试各品种杏果实中类黄酮、总酚、三萜酸含量均在果实发育早期(花后30 d)最低,其后随着果实的发育呈先上升后下降趋势。2.不同品种杏果实发育过程中类黄酮、总酚和三萜酸含量存在极显著差异(P<0.01)。‘华县大接杏’在花后54 d类黄酮和总酚含量均达到最高,‘沙金红’在花后68 d三萜酸含量达到最高。3.供试杏果实的类黄酮、总酚及三萜酸的含量与抗氧化活性均存在相关性,其中杏果实中类黄酮和总酚的含量呈极显著正相关(r=0.882,P<0.01);杏提取物清除·OH的能力和清除·O2-的能力呈显著相关(r=0.376,P<0.05)。类黄酮、总酚含量与杏果实提取物清除DPPH·的能力呈极显著正相关(r=0.488、0.608,P<0.01);三萜酸含量与杏果实提取物清除·OH的能力呈极显著正相关(r=0.494,P<0.01)。【结论】不同品种杏果实发育过程中类黄酮、总酚含量与杏果实提取物清除DPPH·的能力呈显著正相关,三萜酸含量与杏果实提取物清除·OH的能力呈极显著正相关,说明类黄酮、总酚和三萜酸是杏果实中主要的抗氧化活性成分。‘华县大接杏’具有较高的类黄酮、总酚含量和清除DPPH·的能力,‘沙金红’具有较高的三萜酸含量和清除·OH的能力,均是功能性杏果及杏加工制品选择的理想品种。
【Objective】In order to discover and analyze the dynamic changes in the contents of flavanoids,total phenolics and triterpenoidic acids, and analyze the relationship of the contents of flavanoids, total phenolics and triterpenoidic acids with antioxidant capacity during the development of fruits of different apricot cultivars.【Methods】The contents of flavanoids, total phenolics and triterpenoidic acids as well as antioxidant capacity of fruits of all tested six cultivars including early maturing cultivars of‘Golden-sun'and‘Zaojinyan', medium maturing cultivar of‘Katy', medium-late maturing cultivars of‘Huaxiandajiexing'and‘Shajinhong', and late maturing cultivar of‘Jinxiang'. They were determined at eight different stages of fruit development, i.e. 30, 36, 42, 48, 54, 61, 68, and 75 days after blooming. The antioxidant capacity was evaluated by scavenging hydroxy radical(· OH), 1,1- diphenyl- 2- picrylhydrazyl radical(DPPH·) and superoxide anion radical(·O2-). Four grams of chopped apricot fruit sample was put in 10 m L centrifuge tube, added with 4 m L of 80% methyl alcohol as extraction solvent, ultrasonic extracted for10 min at 30 ℃, and centrifuged for 10 min at 9 000 r·min-1, at 4 ℃ in dark. The supernatant was collected. The residues in the centrifuge tube was extracted twice with 4 m L of 80% methyl alcohol, and the extract solution were pooled, diluted to 10 m L with methyl alcohol and cryopreserved at-80 ℃. The apricot fruit extracts were used to analyze and determine the contents of flavanoids, total phenolics and triterpenoidic acids as well as scavenging capacities of ·OH, DPPH· and ·O2-during the development of apricot fruits. The difference in the contents of flavanoids, total phenolics and triterpenoidic acids as well as antioxidant capacity in all tested six cultivars were compared, and the correlation of the contents of flavanoids,total phenolics and triterpenoidic acids with antioxidant capacity were analyzed.【Results】The results indicated that the contents of flavanoids, total phenolics, triterpenoidic acids in all the tested cultivars were lowest at 30 days after blooming. The contents of flavanoids, total phenolics and triterpenoidic acids increased most rapidly in the early stage of rapid fruit growth, while in the second stage of rapid fruit growth with rapid increase in fruit weight, the contents of flavanoids, total phenolics and triterpenoidic acids decreased rapidly. Thereafter, the contents of flavanoids, total phenolics and triterpenoidic acids showed a descending trend with fruit development, and finally, became relatively stable. The contents of flavanoids,total phenolics and triterpenoidic acids differed significantly among different cultivars(P<0.01). There was a peak value of the contents of flavanoids, total phenolics and triterpenoidic acids in all the six cultivars during fruit development. The late maturing cultivar‘Huaxiandajiexing'had maximum contents of flavanoids and total phenolics at 54 d after blooming, another late maturing cultivar,‘Shajinhong', had maximum content of triterpenoidic acids at 68 d after blooming. Correlation analysis revealed that there was a significant positive correlation between the contents of flavanoids and total phenolics(r=0.882, P<0.01). There was also a positive correlation between the scavenging capacities of hydroxy radical(·OH)and the superoxide anion radical(·O2-)(r=0.882, P<0.05). Significant positive correlations were found between the scavenging capacities of DPPH· and the contents of flavanoids(r=0.488; P<0.01) and total phenolics(0.608; P<0.01), respectively. There was also a significant positive correlation between the capacity of scavenging ·OH and the contents of triterpenoidic acids(r=0.494; P<0.01). However, the correlations of scavenging capacities of DPPH· and ·O2-with triterpenoidic acid contents were not significant.【Conclusion】The growth curve of fresh weight of apricot fruits was double sigmoid, which has two rapid periods of fruit growth. The growth pattern of double sigmoid curve was basically consistent with the contents of flavanoids, total phenolics and triterpenoidic acids. At the first stage of rapid growth, the contents of flavanoids, total phenolics and triterpenoidic acids accumulated rapidly and the displayed a descending trend in the following fruit development. But there was a significant difference among the tested apricot cultivars. The contents of flavanoids, total phenolics and triterpenoidic acids were significantly different among different cultivars. The contents of flavanoids and total phenolics were significantly correlated with scavenging capacity of DPPH·, and the content of triterpenoidic acids was significantly correlated with scavenging capacity ·OH, indicating that flavanoids, total phenolics and triterpenoidic acids are the major determinants of antioxidant capacity in apricot fruit. Furthermore, high contents of flavanoids, total phenolics and strong scavenging capacity of DPPH· were found in the late maturing cultivar of‘Huaxiandajiexing'fruit, while the late maturing cultivar of‘Shajinhong'fruit contains high content of triterpenoidic acids and has high capacity of scavenging ·OH. They are preferred apricot varieties for processing product with health benefits.
【Objective】In order to discover and analyze the dynamic changes in the contents of flavanoids,total phenolics and triterpenoidic acids, and analyze the relationship of the contents of flavanoids, total phenolics and triterpenoidic acids with antioxidant capacity during the development of fruits of different apricot cultivars.【Methods】The contents of flavanoids, total phenolics and triterpenoidic acids as well as antioxidant capacity of fruits of all tested six cultivars including early maturing cultivars of‘Golden-sun'and‘Zaojinyan', medium maturing cultivar of‘Katy', medium-late maturing cultivars of‘Huaxiandajiexing'and‘Shajinhong', and late maturing cultivar of‘Jinxiang'. They were determined at eight different stages of fruit development, i.e. 30, 36, 42, 48, 54, 61, 68, and 75 days after blooming. The antioxidant capacity was evaluated by scavenging hydroxy radical(· OH), 1,1- diphenyl- 2- picrylhydrazyl radical(DPPH·) and superoxide anion radical(·O2-). Four grams of chopped apricot fruit sample was put in 10 m L centrifuge tube, added with 4 m L of 80% methyl alcohol as extraction solvent, ultrasonic extracted for10 min at 30 ℃, and centrifuged for 10 min at 9 000 r·min-1, at 4 ℃ in dark. The supernatant was collected. The residues in the centrifuge tube was extracted twice with 4 m L of 80% methyl alcohol, and the extract solution were pooled, diluted to 10 m L with methyl alcohol and cryopreserved at-80 ℃. The apricot fruit extracts were used to analyze and determine the contents of flavanoids, total phenolics and triterpenoidic acids as well as scavenging capacities of ·OH, DPPH· and ·O2-during the development of apricot fruits. The difference in the contents of flavanoids, total phenolics and triterpenoidic acids as well as antioxidant capacity in all tested six cultivars were compared, and the correlation of the contents of flavanoids,total phenolics and triterpenoidic acids with antioxidant capacity were analyzed.【Results】The results indicated that the contents of flavanoids, total phenolics, triterpenoidic acids in all the tested cultivars were lowest at 30 days after blooming. The contents of flavanoids, total phenolics and triterpenoidic acids increased most rapidly in the early stage of rapid fruit growth, while in the second stage of rapid fruit growth with rapid increase in fruit weight, the contents of flavanoids, total phenolics and triterpenoidic acids decreased rapidly. Thereafter, the contents of flavanoids, total phenolics and triterpenoidic acids showed a descending trend with fruit development, and finally, became relatively stable. The contents of flavanoids,total phenolics and triterpenoidic acids differed significantly among different cultivars(P<0.01). There was a peak value of the contents of flavanoids, total phenolics and triterpenoidic acids in all the six cultivars during fruit development. The late maturing cultivar‘Huaxiandajiexing'had maximum contents of flavanoids and total phenolics at 54 d after blooming, another late maturing cultivar,‘Shajinhong', had maximum content of triterpenoidic acids at 68 d after blooming. Correlation analysis revealed that there was a significant positive correlation between the contents of flavanoids and total phenolics(r=0.882, P<0.01). There was also a positive correlation between the scavenging capacities of hydroxy radical(·OH)and the superoxide anion radical(·O2-)(r=0.882, P<0.05). Significant positive correlations were found between the scavenging capacities of DPPH· and the contents of flavanoids(r=0.488; P<0.01) and total phenolics(0.608; P<0.01), respectively. There was also a significant positive correlation between the capacity of scavenging ·OH and the contents of triterpenoidic acids(r=0.494; P<0.01). However, the correlations of scavenging capacities of DPPH· and ·O2-with triterpenoidic acid contents were not significant.【Conclusion】The growth curve of fresh weight of apricot fruits was double sigmoid, which has two rapid periods of fruit growth. The growth pattern of double sigmoid curve was basically consistent with the contents of flavanoids, total phenolics and triterpenoidic acids. At the first stage of rapid growth, the contents of flavanoids, total phenolics and triterpenoidic acids accumulated rapidly and the displayed a descending trend in the following fruit development. But there was a significant difference among the tested apricot cultivars. The contents of flavanoids, total phenolics and triterpenoidic acids were significantly different among different cultivars. The contents of flavanoids and total phenolics were significantly correlated with scavenging capacity of DPPH·, and the content of triterpenoidic acids was significantly correlated with scavenging capacity ·OH, indicating that flavanoids, total phenolics and triterpenoidic acids are the major determinants of antioxidant capacity in apricot fruit. Furthermore, high contents of flavanoids, total phenolics and strong scavenging capacity of DPPH· were found in the late maturing cultivar of‘Huaxiandajiexing'fruit, while the late maturing cultivar of‘Shajinhong'fruit contains high content of triterpenoidic acids and has high capacity of scavenging ·OH. They are preferred apricot varieties for processing product with health benefits.
引文
[1]杨丽,孙浩元,张俊环,王玉柱.我国杏育种研究进展[J].北方园艺,2011(12):170-172.YANG Li,SUN Haoyuan,ZHANG Jun huan,WANG Yuzhu.Brief Introduction of Research Progress on the Breeding of Apricot in China[J].Northern Horticulture,2011(12):170-172.
[2]赵锋,刘威生,刘宁,郁香荷,孙猛,张玉萍,周晏起.我国杏种质资源及遗传育种研究新进展[J].果树学报,2005,22(6):687-690.ZHAO Feng,LIU Weisheng,LIU Ning,YU Xianghe,SUN Meng,ZHANG Yuping,ZHOU Yanqi.Reviews of the apricot germplasm resources and genetic breeding in China[J].Journal of Fruit Science,2005,22(6):687-690.
[3]王玉柱,孙浩元,杨丽.国内外杏研究最新进展[J].北方果树,2003,2(3):1-2.WANG Yuzhu,SUN Haoyuan,YANG Li.The lastest research progress of apricot in domestic and international[J].Northern Fruits,2003,2(3):1-2.
[4]彭晓莉,廖康,贾杨,刘欢,马微,徐乐.9个新疆杏品种间杂交亲和性研究[J].果树学报,2015,32(2):192-199.PENG Xiaoli,LIAO Kang,JIA Yang,LIU Huan,MA Wei,XU Le.Study on the cross compatibility among 9 apricot cultivars in Xinjiang[J].Journal of Fruit Science,2015,32(2):192-199.
[5]张加延,张钊.中国果树志-杏卷[M].北京:中国林业出版社,2003:93-559.ZHANG Jiayan,ZHANG Zhao.China fruit-plant monograph Apricot flora[M].Beijing:China Forestry Press,2003:93-559.
[6]LIU W H,LIU N,YU X,ZHANG Y,SUN M,XU M.Apricot germplasm resources and their utilization in China[J].Acta Horticulturae,2010,862:45-49.
[7]赵海娟,刘威生,刘宁,张玉萍,章秋平,刘硕.普通杏(Prunus armeniaca)种质资源数量性状的遗传多样性分析[J].果树学报,2014,31(1):20-29.ZHAO Haijuan,LIU Weisheng,LIU Ning,ZHANG Yuping,ZHANG Qiuping,LIU Shuo.Genetic diversity analysis for the quantitative traits of common apricot(Prunus armeniaca)germplasm[J].Journal of Fruit Science,2014,31(1):20-29..
[8]张加延.中国李杏资源及开发利用研究[M].北京:中国林业出版社,1999.ZHANG Jiayan.Development and utilization research of apricot and plum resources in China[M].Beijing:China Forestry Publishing House,1999.
[9]郭长江,徐静,韦京豫,杨继军,吴健全.我国常见水果类黄酮物质的含量[J].营养学报,2008,30(2):130-135.GUO Changjiang,XU Jing,WEI Jingyu,YANG Jijun,WU Jianquan.The contents of flavonoid of common fruits in China[J].Acta Nutrimenta Sinica,2008,30(2):130-135.
[10]TOGASHI H,SHINZAWA H,MATSUO T,TAKAHASHI T,AOYAMA M,OIKAWA K,KAMADA H.Analysis of hepatic oxidative stress status by electron spin resonance spectroscopy and imaging[J].Free Radical Biology and Medicine,2000,28(6):846-853.
[11]UGRAS M Y,KURUS M,ATES B,SOYLEMEZ H,OTLU A,YILMAZ I.Prunus armeniaca L(apricot)protects rat testes from detrimental effects of low-dose x-rays[J].Nutrition Research,2010,30:200-208.
[12]HEIM K E,TAGLIAFERRO A R,BOLIYA D J.Flavonoid antioxidants:chemistry,metabolism and structure-activity relationships[J].Journal of Nutritional Biochemistry,2002,13:572-584.
[13]宋立江,狄莹,石碧.植物多酚研究与利用的意义及发展趋势[J].化学进展,2000,12(2):161-169.SONG Lijiang,DI Ying,SHI Bi.The significance and development trend in research of plant polypehnolics[J].Progress in Chemistry,2000,12(2):161-169.
[14]孙达旺.植物单宁化学[M].北京:中国林业出版社,1992:3.SUN Dawang.Plant Tannin Chemistry[M].Beijing:China Forestry Publishing House,1992:3.
[15]MIDDLETON E,JR,KANDASWAMI C,THEOHARIDES T C.The effects of plant flavonoids on mammalian cells:implications for inflammation,heart disease,and cancer[J].Pharmacol Reviews,2000,52:673-751.
[16]郭长江.食物类黄酮物质的营养学意义[J].中国食物与营养,2004(3):38-40.GUO Changjiang.Nutrition significance of food flavonoids[J].Food and Nutrition in China,2004(3):38-40.
[17]于希志,徐秋萍,金锡凤.杏果实发育的研究[J].果树科学,1990,7(4):227-230.YU Xizhi,XU Qiuping,JIN Xifeng.Research in development of apricot fruits[J].Journal of Fruit Science,1990,7(4):227-230.
[18]王玉柱,胡南,刘奇志,杨丽,石宏.若干杏品种果肉色泽与维生素含量的测定[J].果树科学,1999,16(1):51-54.WANG Yuzhu,HU Nan,LIU Qizhi,YANG Li,SHI Hong.Study on the vitamin content in different flesh color varieties of apricot[J].Journal of Fruit Science,1999,16(1):51-54.
[19]DRAGOVIC-UZELAC V,DELONGA K,LEVAJ B,DJAKOVIC S,POSPISIL J.Pehnolicic profiles of raw apricots,pumpkins and their purees in the evaluation of apricot nectar and jam authenticity[J].Journal of Agricultural and Food Chemistry,2005,53:4836-4842.
[20]DRAGOVIC-UZELAC V,LEVAJ B,MRKIC V,BURSAC D,BORAS M.The content of polypehnolics and carotenoids in three apricot cultivars depending on stage of maturity and geographical region[J].Food Chemistry,2007,102:966-975.
[21]冯立娟,苑兆和,尹燕雷,招雪晴,王金政,李英朋.2个杏品种不同成熟期果实品质变化研究[J].中国农学通报,2010,26(16):206-210.FENG Lijuan,YUAN Zhaohe,YIN Yanlei,ZHAO Xueqing,WANG Jinzheng,LI Yingpeng.Studies on the change of fruit quality target of two apricot cultivar in different maturation period[J].Chinese Agricultural Science Bulletin,2010,26(16):206-210.
[22]DROGOUDI P D,MICHAILIDIS Z,PANTELIDIS G.Peel and flesh antioxidant content and harvest quality characteristics of seven apples cultivars[J].Scientia Horticulturae,2008,115:149-153.
[23]VIEIRA F G K,BORGES G D C,COPETTI C,DI PIETRO P F,NUNES E D,FETT R.Pehnolicic compounds and antioxidant activity of apple flesh and peel of eleven cultivars grown in Brazil[J].Scientia Horticulturae,2011,128:261-266.
[24]TIAN Li,WANG Sibao,PAN Qinhong,ZHENG Yanjun,HUANG Weidong.A novel plastid localization of chalcone synthase in developing grape berry[J].Plant Science,2008,175(3):431-436.
[25]WANG Wei,Zhang Ping,PAN Qiuhong,ZHAN Jicheng,HUANG Weidong.Gene transcript accumulation,tissue and subcellular localization of anthocyanidin synthase(ANS)in developing grape berries[J].Plant Science,2010,179:103-113.
[26]王文祥,顾振纶.比色法测定山楂总三萜酸的含量[J].中国野生植物资源,2001,20(5):41-42.WANG Wenxiang,GU Zhenguan.Determination of total triterpenoidic acids contents of hawthorn fruit with colorimetry[J].Chinese Wild Plant Resources,2001,20(5):41-42.
[27]胡丽娜,张春岭,刘慧,吕真真,陈大磊,焦中高.短波紫外线处理对采后山楂果营养品质及抗氧化活性的影响[J].食品工业科技,http://www.cnki.net/kcms/detail/11.1759.TS.20150723.1310.016.html[2015-07-23]HU Lina,ZHANG Chunling,LIU Hui,LV Zhenzhen,CHEN Dalei,JIAO Zhonggao.Effect of UV-C irradiation on nutritive qualities and antioxidant activity of postharvest hawthorn fruit[J].Science and Technology of Food Industry,http://www.cnki.net/kcms/detail/11.1759.TS.20150723.1310.016.html[2015-07-23].
[28]周丹蓉,方智振,廖汝玉,叶新福,姜翠翠,潘少霖.李果皮花色素苷、类黄酮和类胡萝卜素含量及抗氧化性研究[J].营养学报,2013,35(6):571-575.ZHOU Danrong,FANG Zhizhen,LIAO Ruyu,YE Xinfu,JIANG Cuicui,PAN Shaolin.Contents of anthocy-anin,flavonoids and carotenoids and antioxidant capacity of plum peels[J].Acta Nutrimenta Sinica,2013,35(6):571-575.
[29]张俊环,杨丽,孙浩元,王玉柱.不同品种杏果实发育进程中多酚与类黄酮物质含量的变化[J].北方园艺,2012(24):1-5.ZHANG Junhuan,YANG Li,SUN Haoyuan,WANG Yuzhu.Changes of total pehnolics and flavonoids in apricot peel and pulp of different cultivars during fruit development[J].Northern Horticulture,2012(24):1-5.
[30]唐巧玉,周毅峰,朱玉昌,云鑫.金橘皮中黄酮类物质的提取及其体外抗氧化活性研究[J].农业工程学报,2008,24(6):258-261.TANG Qiaoyu,ZHOU Yifeng,ZHU Yuchang,YUN Xin.Extraction of flavone compounds from Fortunella margarita peel and its antioxidation in vitro[J].Transactions of the CSAE,2008,24(6):258-261.
[31]BONILLA E P,AKOH C C,SELLAPPAN S,KREWER G.Pehnolicic content and antioxidant capacity of Muscadine grapes[J].Journal of Agricultural Food Chemistry,2003,51(18):5497-5503.
[32]周巧霞,张经硕,顾明,顾振纶.测定山碴及提取物总三萜酸的含量[J].中国野生植物资源,2004,23(5):43-44.ZHOU Qiaoxia,ZHANG Jingshuo,GU Ming,GU Zhenguan.Determination total triterpenoidic acids contents of hawthorn fruit[J].Chinese Wild Plant Resources,2004,23(5):43-44.
[33]徐建国,胡青平.决明子水提物体外清除自由基活性的研究[J].食品科学,2006,27(6):73-76.XU Jianguo,HU Qingping.Study on free radical scavenging capacity by cassia seed water extract in vitro[J].Food Science,2006,27(6):73-76.
[34]郭雪峰,岳永德,汤锋,王进,姚曦.用清除超氧阴离子自由基法评价竹叶提取物抗氧化能力[J].光谱学与光谱分析,2008,28(8):1823-1826.GUO Xuefeng,YUE Yongde,TANG Feng,WANG Jin,YAO Xi.Detection of antioxidative capacity of bamboo leaf extract by scavenging superoxide anion free radical[J].Spectroscopy and Spectral Analysis,2008,28(8):1823-1826.
[35]韦献雅,殷丽琴,钟成,章明海,牛应泽.DPPH法评价抗氧化活性研究进展[J].食品科学,2014,35(9):317-322.WEI Xianya,YIN Liqin,ZHONG Cheng,ZHANG Minghai,NIU Yingze.Advances in the DPPH radical scavenging assay for antioxidant activity evaluat[J].Food Science,2014,35(9):317-322.
[36]CHEN J Y,WEN P F,KONG W F,PAN Q H,WAN S B,HUANG W D.Changes and subcellular localizations of the enzymes involved in phenylpropanoid metabolism during grape berry development[J].Journal of Plant Physiology,2006,163(2):115-127.
[37]冯晨静,关军锋,杨建民,张元慧,赵树堂,王玉涛.草莓果实成熟期花青苷、酚类物质和类黄酮含量的变化[J].果树学报,2003,20(3):199-201.FENG Chenjing,GUAN Junfeng,YANG Jianmin,ZHANG Yuanhui,ZHAO Shutang,WANG Yutao.Changes of the content of anthocyanin,pehnolicic and flavonoid compounds in strawberries during the maturation[J].Journal of Fruit Science,2003,20(3):199-201.
[38]CELLI G B,PEREIRA-NETTO A B,BETA T.Comparative analysis of total pehnolicic content,antioxidant activity,and flavonoids profile of fruits from two varieties of Brazilian cherry(Eugenia uniflora L.)throughout the fruit developmental stages[J].Food Research International,2011,44(8):2442-2451.
[39]刘奕琳,王振宇.蓝靛果中花色苷含量的测定及其体外抗氧化性[J].中国林副特产,2011(5):14-17.LIU Liyin,WANG Zhenyu.Determination the anthocyanin content in Lonicera edulis by PH-differential spectrophotometry and antioxidance[J].Forest BY-product and Speciality in China,2011(5):14-17.
[2]赵锋,刘威生,刘宁,郁香荷,孙猛,张玉萍,周晏起.我国杏种质资源及遗传育种研究新进展[J].果树学报,2005,22(6):687-690.ZHAO Feng,LIU Weisheng,LIU Ning,YU Xianghe,SUN Meng,ZHANG Yuping,ZHOU Yanqi.Reviews of the apricot germplasm resources and genetic breeding in China[J].Journal of Fruit Science,2005,22(6):687-690.
[3]王玉柱,孙浩元,杨丽.国内外杏研究最新进展[J].北方果树,2003,2(3):1-2.WANG Yuzhu,SUN Haoyuan,YANG Li.The lastest research progress of apricot in domestic and international[J].Northern Fruits,2003,2(3):1-2.
[4]彭晓莉,廖康,贾杨,刘欢,马微,徐乐.9个新疆杏品种间杂交亲和性研究[J].果树学报,2015,32(2):192-199.PENG Xiaoli,LIAO Kang,JIA Yang,LIU Huan,MA Wei,XU Le.Study on the cross compatibility among 9 apricot cultivars in Xinjiang[J].Journal of Fruit Science,2015,32(2):192-199.
[5]张加延,张钊.中国果树志-杏卷[M].北京:中国林业出版社,2003:93-559.ZHANG Jiayan,ZHANG Zhao.China fruit-plant monograph Apricot flora[M].Beijing:China Forestry Press,2003:93-559.
[6]LIU W H,LIU N,YU X,ZHANG Y,SUN M,XU M.Apricot germplasm resources and their utilization in China[J].Acta Horticulturae,2010,862:45-49.
[7]赵海娟,刘威生,刘宁,张玉萍,章秋平,刘硕.普通杏(Prunus armeniaca)种质资源数量性状的遗传多样性分析[J].果树学报,2014,31(1):20-29.ZHAO Haijuan,LIU Weisheng,LIU Ning,ZHANG Yuping,ZHANG Qiuping,LIU Shuo.Genetic diversity analysis for the quantitative traits of common apricot(Prunus armeniaca)germplasm[J].Journal of Fruit Science,2014,31(1):20-29..
[8]张加延.中国李杏资源及开发利用研究[M].北京:中国林业出版社,1999.ZHANG Jiayan.Development and utilization research of apricot and plum resources in China[M].Beijing:China Forestry Publishing House,1999.
[9]郭长江,徐静,韦京豫,杨继军,吴健全.我国常见水果类黄酮物质的含量[J].营养学报,2008,30(2):130-135.GUO Changjiang,XU Jing,WEI Jingyu,YANG Jijun,WU Jianquan.The contents of flavonoid of common fruits in China[J].Acta Nutrimenta Sinica,2008,30(2):130-135.
[10]TOGASHI H,SHINZAWA H,MATSUO T,TAKAHASHI T,AOYAMA M,OIKAWA K,KAMADA H.Analysis of hepatic oxidative stress status by electron spin resonance spectroscopy and imaging[J].Free Radical Biology and Medicine,2000,28(6):846-853.
[11]UGRAS M Y,KURUS M,ATES B,SOYLEMEZ H,OTLU A,YILMAZ I.Prunus armeniaca L(apricot)protects rat testes from detrimental effects of low-dose x-rays[J].Nutrition Research,2010,30:200-208.
[12]HEIM K E,TAGLIAFERRO A R,BOLIYA D J.Flavonoid antioxidants:chemistry,metabolism and structure-activity relationships[J].Journal of Nutritional Biochemistry,2002,13:572-584.
[13]宋立江,狄莹,石碧.植物多酚研究与利用的意义及发展趋势[J].化学进展,2000,12(2):161-169.SONG Lijiang,DI Ying,SHI Bi.The significance and development trend in research of plant polypehnolics[J].Progress in Chemistry,2000,12(2):161-169.
[14]孙达旺.植物单宁化学[M].北京:中国林业出版社,1992:3.SUN Dawang.Plant Tannin Chemistry[M].Beijing:China Forestry Publishing House,1992:3.
[15]MIDDLETON E,JR,KANDASWAMI C,THEOHARIDES T C.The effects of plant flavonoids on mammalian cells:implications for inflammation,heart disease,and cancer[J].Pharmacol Reviews,2000,52:673-751.
[16]郭长江.食物类黄酮物质的营养学意义[J].中国食物与营养,2004(3):38-40.GUO Changjiang.Nutrition significance of food flavonoids[J].Food and Nutrition in China,2004(3):38-40.
[17]于希志,徐秋萍,金锡凤.杏果实发育的研究[J].果树科学,1990,7(4):227-230.YU Xizhi,XU Qiuping,JIN Xifeng.Research in development of apricot fruits[J].Journal of Fruit Science,1990,7(4):227-230.
[18]王玉柱,胡南,刘奇志,杨丽,石宏.若干杏品种果肉色泽与维生素含量的测定[J].果树科学,1999,16(1):51-54.WANG Yuzhu,HU Nan,LIU Qizhi,YANG Li,SHI Hong.Study on the vitamin content in different flesh color varieties of apricot[J].Journal of Fruit Science,1999,16(1):51-54.
[19]DRAGOVIC-UZELAC V,DELONGA K,LEVAJ B,DJAKOVIC S,POSPISIL J.Pehnolicic profiles of raw apricots,pumpkins and their purees in the evaluation of apricot nectar and jam authenticity[J].Journal of Agricultural and Food Chemistry,2005,53:4836-4842.
[20]DRAGOVIC-UZELAC V,LEVAJ B,MRKIC V,BURSAC D,BORAS M.The content of polypehnolics and carotenoids in three apricot cultivars depending on stage of maturity and geographical region[J].Food Chemistry,2007,102:966-975.
[21]冯立娟,苑兆和,尹燕雷,招雪晴,王金政,李英朋.2个杏品种不同成熟期果实品质变化研究[J].中国农学通报,2010,26(16):206-210.FENG Lijuan,YUAN Zhaohe,YIN Yanlei,ZHAO Xueqing,WANG Jinzheng,LI Yingpeng.Studies on the change of fruit quality target of two apricot cultivar in different maturation period[J].Chinese Agricultural Science Bulletin,2010,26(16):206-210.
[22]DROGOUDI P D,MICHAILIDIS Z,PANTELIDIS G.Peel and flesh antioxidant content and harvest quality characteristics of seven apples cultivars[J].Scientia Horticulturae,2008,115:149-153.
[23]VIEIRA F G K,BORGES G D C,COPETTI C,DI PIETRO P F,NUNES E D,FETT R.Pehnolicic compounds and antioxidant activity of apple flesh and peel of eleven cultivars grown in Brazil[J].Scientia Horticulturae,2011,128:261-266.
[24]TIAN Li,WANG Sibao,PAN Qinhong,ZHENG Yanjun,HUANG Weidong.A novel plastid localization of chalcone synthase in developing grape berry[J].Plant Science,2008,175(3):431-436.
[25]WANG Wei,Zhang Ping,PAN Qiuhong,ZHAN Jicheng,HUANG Weidong.Gene transcript accumulation,tissue and subcellular localization of anthocyanidin synthase(ANS)in developing grape berries[J].Plant Science,2010,179:103-113.
[26]王文祥,顾振纶.比色法测定山楂总三萜酸的含量[J].中国野生植物资源,2001,20(5):41-42.WANG Wenxiang,GU Zhenguan.Determination of total triterpenoidic acids contents of hawthorn fruit with colorimetry[J].Chinese Wild Plant Resources,2001,20(5):41-42.
[27]胡丽娜,张春岭,刘慧,吕真真,陈大磊,焦中高.短波紫外线处理对采后山楂果营养品质及抗氧化活性的影响[J].食品工业科技,http://www.cnki.net/kcms/detail/11.1759.TS.20150723.1310.016.html[2015-07-23]HU Lina,ZHANG Chunling,LIU Hui,LV Zhenzhen,CHEN Dalei,JIAO Zhonggao.Effect of UV-C irradiation on nutritive qualities and antioxidant activity of postharvest hawthorn fruit[J].Science and Technology of Food Industry,http://www.cnki.net/kcms/detail/11.1759.TS.20150723.1310.016.html[2015-07-23].
[28]周丹蓉,方智振,廖汝玉,叶新福,姜翠翠,潘少霖.李果皮花色素苷、类黄酮和类胡萝卜素含量及抗氧化性研究[J].营养学报,2013,35(6):571-575.ZHOU Danrong,FANG Zhizhen,LIAO Ruyu,YE Xinfu,JIANG Cuicui,PAN Shaolin.Contents of anthocy-anin,flavonoids and carotenoids and antioxidant capacity of plum peels[J].Acta Nutrimenta Sinica,2013,35(6):571-575.
[29]张俊环,杨丽,孙浩元,王玉柱.不同品种杏果实发育进程中多酚与类黄酮物质含量的变化[J].北方园艺,2012(24):1-5.ZHANG Junhuan,YANG Li,SUN Haoyuan,WANG Yuzhu.Changes of total pehnolics and flavonoids in apricot peel and pulp of different cultivars during fruit development[J].Northern Horticulture,2012(24):1-5.
[30]唐巧玉,周毅峰,朱玉昌,云鑫.金橘皮中黄酮类物质的提取及其体外抗氧化活性研究[J].农业工程学报,2008,24(6):258-261.TANG Qiaoyu,ZHOU Yifeng,ZHU Yuchang,YUN Xin.Extraction of flavone compounds from Fortunella margarita peel and its antioxidation in vitro[J].Transactions of the CSAE,2008,24(6):258-261.
[31]BONILLA E P,AKOH C C,SELLAPPAN S,KREWER G.Pehnolicic content and antioxidant capacity of Muscadine grapes[J].Journal of Agricultural Food Chemistry,2003,51(18):5497-5503.
[32]周巧霞,张经硕,顾明,顾振纶.测定山碴及提取物总三萜酸的含量[J].中国野生植物资源,2004,23(5):43-44.ZHOU Qiaoxia,ZHANG Jingshuo,GU Ming,GU Zhenguan.Determination total triterpenoidic acids contents of hawthorn fruit[J].Chinese Wild Plant Resources,2004,23(5):43-44.
[33]徐建国,胡青平.决明子水提物体外清除自由基活性的研究[J].食品科学,2006,27(6):73-76.XU Jianguo,HU Qingping.Study on free radical scavenging capacity by cassia seed water extract in vitro[J].Food Science,2006,27(6):73-76.
[34]郭雪峰,岳永德,汤锋,王进,姚曦.用清除超氧阴离子自由基法评价竹叶提取物抗氧化能力[J].光谱学与光谱分析,2008,28(8):1823-1826.GUO Xuefeng,YUE Yongde,TANG Feng,WANG Jin,YAO Xi.Detection of antioxidative capacity of bamboo leaf extract by scavenging superoxide anion free radical[J].Spectroscopy and Spectral Analysis,2008,28(8):1823-1826.
[35]韦献雅,殷丽琴,钟成,章明海,牛应泽.DPPH法评价抗氧化活性研究进展[J].食品科学,2014,35(9):317-322.WEI Xianya,YIN Liqin,ZHONG Cheng,ZHANG Minghai,NIU Yingze.Advances in the DPPH radical scavenging assay for antioxidant activity evaluat[J].Food Science,2014,35(9):317-322.
[36]CHEN J Y,WEN P F,KONG W F,PAN Q H,WAN S B,HUANG W D.Changes and subcellular localizations of the enzymes involved in phenylpropanoid metabolism during grape berry development[J].Journal of Plant Physiology,2006,163(2):115-127.
[37]冯晨静,关军锋,杨建民,张元慧,赵树堂,王玉涛.草莓果实成熟期花青苷、酚类物质和类黄酮含量的变化[J].果树学报,2003,20(3):199-201.FENG Chenjing,GUAN Junfeng,YANG Jianmin,ZHANG Yuanhui,ZHAO Shutang,WANG Yutao.Changes of the content of anthocyanin,pehnolicic and flavonoid compounds in strawberries during the maturation[J].Journal of Fruit Science,2003,20(3):199-201.
[38]CELLI G B,PEREIRA-NETTO A B,BETA T.Comparative analysis of total pehnolicic content,antioxidant activity,and flavonoids profile of fruits from two varieties of Brazilian cherry(Eugenia uniflora L.)throughout the fruit developmental stages[J].Food Research International,2011,44(8):2442-2451.
[39]刘奕琳,王振宇.蓝靛果中花色苷含量的测定及其体外抗氧化性[J].中国林副特产,2011(5):14-17.LIU Liyin,WANG Zhenyu.Determination the anthocyanin content in Lonicera edulis by PH-differential spectrophotometry and antioxidance[J].Forest BY-product and Speciality in China,2011(5):14-17.