应力加载系统在细胞力学研究与教学中的应用
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摘要
细胞作为人体结构和功能的基本组成单位,在机体中受到各种形式的力学刺激。体外培养细胞的应力加载技术和装置是细胞力学研究的重要基础和手段。对牵张应力加载系统在心血管疾病、创伤修复、干细胞分化等生物医学工程领域的应用进行了介绍。牵张应力加载系统可以模拟心脏、血管、韧带、肌肉、骨骼、肺等细胞所处的力学环境,采用基底应变加载技术对体外培养细胞实现静态或周期性的力学刺激,从而引起细胞产生生化反应应答。为适应重点实验室开放和高校实验教学改革的需求,将该系统用于实验教学,取得了良好的教学效果,为培养和提升学生的科研实践能力和创新能力提供了重要平台。
As the basic unit of human structure and function,cells are subjected to amplitude mechanical stimulation in the body. Loading techniques and devices based on substrate-tension to in vitro cultured cells are the important basis and method of cellular mechanics research. Applications of mechanical tension loading system in the fields of cardiovascular diseases,trauma care,stem cell differentiation etc. are introduced in this paper. Mechanical tension loading system could simulate the mechanical environment where cells are located,which are from heart,vascular vessels,ligament,bone,lung etc. The system applies static or cyclic deformation to cells,and induce biochemical changes in response to mechanical tension. Besides,in order to meet the needs of the opening of key laboratories and the reform of experimental teaching in colleges and universities,the system was applied in the experimental teaching,which obtained favorable teaching effects and provided an important platform for training and promoting students' scientific research practice ability and innovation ability.
As the basic unit of human structure and function,cells are subjected to amplitude mechanical stimulation in the body. Loading techniques and devices based on substrate-tension to in vitro cultured cells are the important basis and method of cellular mechanics research. Applications of mechanical tension loading system in the fields of cardiovascular diseases,trauma care,stem cell differentiation etc. are introduced in this paper. Mechanical tension loading system could simulate the mechanical environment where cells are located,which are from heart,vascular vessels,ligament,bone,lung etc. The system applies static or cyclic deformation to cells,and induce biochemical changes in response to mechanical tension. Besides,in order to meet the needs of the opening of key laboratories and the reform of experimental teaching in colleges and universities,the system was applied in the experimental teaching,which obtained favorable teaching effects and provided an important platform for training and promoting students' scientific research practice ability and innovation ability.
引文
[1]姜宗来.从生物力学到力学生物学的进展[J].力学进展,2017,47(9):309-332.
[2]李开宾,严荣国,徐军.体外细胞培养的加力装置及压力测量的研究[J].中国医学物理学杂志,2014,31(5):5215-5219.
[3]梁向晖,钟伟强,毛秋平.研究生化学实验课程教学改革探讨[J].实验科学与技术,2017,15(1):120-123.
[4]Chua SK,Wang BW,Lien LM,et al.Mechanical stretch inhibits microRNA499 via p53 to regulate calcineurin-A expression in rat cardiomyocytes[J].PLoS One,2016,11(2):1-19.
[5]Fan D,Takawale A,Shen M,et al.A disintegrin and metalloprotease-17 regulates pressure overload-induced myocardial hypertrophy and dysfunction through proteolytic processing of integrinβ1[J].Hypertension,2016,68(4):937-948.
[6]Huang K,Bao H,Yan ZQ,et al.MicroRNA-33 protects against neointimal hyperplasia induced by arterial mechanical stretch in the grafted vein[J].Cardiovasc Res,2017,113(5):488-497.
[7]Dong R,Zhang K,Wang YL,et al.MiR-551b-5p contributes to pathogenesis of vein graft failure via upregulating early growth response-1 expression[J].Chin Med J(Engl),2017,130(13):1578-1585.
[8]陈文琦.动态共培养下前交叉韧带成纤维细胞活性及MMP-2蛋白表达[A].中国生物物理学会.第一届国际暨第十三次中国生物物理学术大会摘要集---S15细胞-分子的力学-生物学-化学耦合[C].中国生物物理学会:中国生物物理学会,2013:1.
[9]Yan Yan,Singh Gurinder K.,Zhang Feifei et al.Comparative study of normal and rheumatoid arthritis fibroblast-like synoviocytes proliferationunder cyclic mechanical stretch:role of prostaglandin e-2[J].Connective tissue research,2012,53(3):246-254.
[10]Wang Y,Xiong Z,Gong W,et al.Expression of heat shock protein 27 correlates with actin cytoskeletal dynamics and contr actility of cultured human bladder smooth muscle cells[J].Exp Cell Res,2015,338(1):39-44.
[11]欧阳宁鹃,张鹏,傅润卿,等.Fox O1在持续张应力促MC3T3-E1细胞成骨向分化中的表达变化[J].医用生物力学,2015,30(2):154-158.166.
[12]Li Haibin,Lei Mingxing,Luo,Ziwei et al.Mechano-growth factor enhances differentiation of bone marrow-derived mesenchymal stem cells[J].Biotechnology Letters,2015,37(11):2341-2348.
[13]欧阳宁鹃,傅润卿,张鹏,等.间歇张应力对骨质疏松大鼠骨髓基质干细胞骨向分化中细胞骨架的影响[J].中国组织工程研究,2014,18(37):5905-5910.
[14]宁尚龙,马信龙,范东伟.机械应力刺激在胸椎黄韧带骨化成骨中的作用[J].解放军预防医学杂志,2017,35(9):1103-1106,1117.
[15]赵艳,王佳帅,郭冬会,等.静态牵张力对人炎症牙周膜干细胞成骨分化及细胞骨架重组的影响[J].牙体牙髓牙周病学杂志,2017,27(4):183-188.
[16]侯文婕,陈静,李文星,等.BMP-2控释涂层对加力状态下牙周膜干细胞成骨分化的影响[J].同济大学学报(医学版),2015,36(5):29-33.
[17]Zeng Z,Jing D,Zhang X,et al.Cyclic mechanical stretch promotes energy metabolism in osteoblast-like cells through an m TOR signaling-associated mechanism[J].Int J Mol Med,2015,36(4):947-956.
[18]孙腊珍,张增明,叶邦角,等.科研能力培养和研究生实验教学体系的构建[J].学位与研究生教育,2010(6):58-60.
[19]刘娜.依托国家重点实验室培养高水平创新型研究生的探索与实践[J].中国基础科学,2014,16(1):38-41.
[20]赵琴,江启峰,刘小兵.依托重点实验室构建地方高校研究生实验教学体系[J].实验技术与管理,2015,32(12):22-25.
[2]李开宾,严荣国,徐军.体外细胞培养的加力装置及压力测量的研究[J].中国医学物理学杂志,2014,31(5):5215-5219.
[3]梁向晖,钟伟强,毛秋平.研究生化学实验课程教学改革探讨[J].实验科学与技术,2017,15(1):120-123.
[4]Chua SK,Wang BW,Lien LM,et al.Mechanical stretch inhibits microRNA499 via p53 to regulate calcineurin-A expression in rat cardiomyocytes[J].PLoS One,2016,11(2):1-19.
[5]Fan D,Takawale A,Shen M,et al.A disintegrin and metalloprotease-17 regulates pressure overload-induced myocardial hypertrophy and dysfunction through proteolytic processing of integrinβ1[J].Hypertension,2016,68(4):937-948.
[6]Huang K,Bao H,Yan ZQ,et al.MicroRNA-33 protects against neointimal hyperplasia induced by arterial mechanical stretch in the grafted vein[J].Cardiovasc Res,2017,113(5):488-497.
[7]Dong R,Zhang K,Wang YL,et al.MiR-551b-5p contributes to pathogenesis of vein graft failure via upregulating early growth response-1 expression[J].Chin Med J(Engl),2017,130(13):1578-1585.
[8]陈文琦.动态共培养下前交叉韧带成纤维细胞活性及MMP-2蛋白表达[A].中国生物物理学会.第一届国际暨第十三次中国生物物理学术大会摘要集---S15细胞-分子的力学-生物学-化学耦合[C].中国生物物理学会:中国生物物理学会,2013:1.
[9]Yan Yan,Singh Gurinder K.,Zhang Feifei et al.Comparative study of normal and rheumatoid arthritis fibroblast-like synoviocytes proliferationunder cyclic mechanical stretch:role of prostaglandin e-2[J].Connective tissue research,2012,53(3):246-254.
[10]Wang Y,Xiong Z,Gong W,et al.Expression of heat shock protein 27 correlates with actin cytoskeletal dynamics and contr actility of cultured human bladder smooth muscle cells[J].Exp Cell Res,2015,338(1):39-44.
[11]欧阳宁鹃,张鹏,傅润卿,等.Fox O1在持续张应力促MC3T3-E1细胞成骨向分化中的表达变化[J].医用生物力学,2015,30(2):154-158.166.
[12]Li Haibin,Lei Mingxing,Luo,Ziwei et al.Mechano-growth factor enhances differentiation of bone marrow-derived mesenchymal stem cells[J].Biotechnology Letters,2015,37(11):2341-2348.
[13]欧阳宁鹃,傅润卿,张鹏,等.间歇张应力对骨质疏松大鼠骨髓基质干细胞骨向分化中细胞骨架的影响[J].中国组织工程研究,2014,18(37):5905-5910.
[14]宁尚龙,马信龙,范东伟.机械应力刺激在胸椎黄韧带骨化成骨中的作用[J].解放军预防医学杂志,2017,35(9):1103-1106,1117.
[15]赵艳,王佳帅,郭冬会,等.静态牵张力对人炎症牙周膜干细胞成骨分化及细胞骨架重组的影响[J].牙体牙髓牙周病学杂志,2017,27(4):183-188.
[16]侯文婕,陈静,李文星,等.BMP-2控释涂层对加力状态下牙周膜干细胞成骨分化的影响[J].同济大学学报(医学版),2015,36(5):29-33.
[17]Zeng Z,Jing D,Zhang X,et al.Cyclic mechanical stretch promotes energy metabolism in osteoblast-like cells through an m TOR signaling-associated mechanism[J].Int J Mol Med,2015,36(4):947-956.
[18]孙腊珍,张增明,叶邦角,等.科研能力培养和研究生实验教学体系的构建[J].学位与研究生教育,2010(6):58-60.
[19]刘娜.依托国家重点实验室培养高水平创新型研究生的探索与实践[J].中国基础科学,2014,16(1):38-41.
[20]赵琴,江启峰,刘小兵.依托重点实验室构建地方高校研究生实验教学体系[J].实验技术与管理,2015,32(12):22-25.