补肾活血法治疗不孕症评述
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摘要
补肾活血法可用于治疗卵巢、输卵管、子宫、免疫等因素所致不孕症患者。其作用机理包括(1)调节卵巢生殖内分泌功能;(2)调节卵巢血流供应;(3)调节卵巢局部微环境;(4)改善子宫内膜形态及发育;(5)改善子宫着床微环境。但由于中药组方复杂、辨证灵活,研究人员各自为营、研究零散、重复性差,难以形成多中心、大样本、系统化的研究和标准化的遣方用药。故今后应根据有关证型的基础方药以及随证加减,形成标准化及量化治疗方案;进一步研究中药的具体有效成分,并分析具体的分子机制。
Bushen Huoxue method can be used to treat infertility patients caused by factors such as ovary,fallopian tube,uterus and immunity. Its mechanism of action includes(1)regulation of ovarian reproductive endocrine function;(2)regulation of ovarian blood flow supply;(3)regulation of ovarian local microenvironment;(4)improvement of endometrial morphology and development;(5)improvement of uterine implantation microenvironment. However,due to the complexity of the traditional Chinese medicine prescription and the flexibility of dialectical differentiation,the researchers are each battalted,with scattered research and poor reproducibility. It is difficult to form multi-center,large-sample,systematic research and standardization of the use of drugs. Therefore,in the future,standardized and quantified treatment schemes should be formed according to the basic prescriptions of relevant syndromes and their additions and subtractions along with syndromes,and specific effective components of traditional Chinese medicine should be further studied,and specific molecular mechanisms should be analyzed.
Bushen Huoxue method can be used to treat infertility patients caused by factors such as ovary,fallopian tube,uterus and immunity. Its mechanism of action includes(1)regulation of ovarian reproductive endocrine function;(2)regulation of ovarian blood flow supply;(3)regulation of ovarian local microenvironment;(4)improvement of endometrial morphology and development;(5)improvement of uterine implantation microenvironment. However,due to the complexity of the traditional Chinese medicine prescription and the flexibility of dialectical differentiation,the researchers are each battalted,with scattered research and poor reproducibility. It is difficult to form multi-center,large-sample,systematic research and standardization of the use of drugs. Therefore,in the future,standardized and quantified treatment schemes should be formed according to the basic prescriptions of relevant syndromes and their additions and subtractions along with syndromes,and specific effective components of traditional Chinese medicine should be further studied,and specific molecular mechanisms should be analyzed.
引文
[1]MENG Q,REN A,ZHANG L,et al. Incidence of infertility and risk factors of impaired fecundity among newly married couples in a Chinese population[J]. Reprod Biomed Online,2015,30(1):92-100.
[2]周猷,庞成,何电,等.我国不孕症研究进展[J].中国计划生育学杂志,2011,19(7):445-446.
[3]马丽亚,李宾玲,张大伟.《傅青主女科》从肾论治不孕症探析[J].中医学报,2015,30(12):1801-1803.
[4]马堃,佟雅婧.从肾虚血瘀论治排卵障碍性不孕[J].中国中药杂志,2017,42(23):4451-4454.
[5]丁键红,叶平.浅析古代医家对不孕症病因病机的认识[J].吉林中医药,2010,30(8):645-646.
[6]郝庆秀,王继峰,牛建昭,等.四物汤及组方中药植物雌激素活性的实验研究[J].中华中医药学刊,2009,27(4):738-741.
[7]秦达念,佘白蓉,佘运初.菟丝子黄酮对实验动物及人绒毛组织生殖功能的影响[J].中药新药与临床药理,2000,11(6):349-351.
[8]韦妍妍,张紫佳,徐颖,等.补骨脂对去卵巢大鼠雌激素样作用研究[J].中国实验方剂学杂志,2011,17(13):158-161.
[9]李艳,于涛,苗明三.淫羊藿的化学、药理与临床应用分析[J].中医学报,2017,32(4):619-622.
[10]高玉桂,王灵芝,唐冀雪.丹参酮的性激素样活性[J].中国医学科学院学报,1980,2(3):189-191.
[11]赵丕文,王大伟,牛建昭,等.红花等10种中药的植物雌激素活性研究[J].中国中药杂志,2007,32(5):436-439.
[12]王艳,周广举,严宗逊,等.川牛膝在去卵巢大鼠体内的雌激素效应及对脂代谢的影响[J].中国妇幼保健,2015,30(29):5063-5066.
[13]马堃,刘雁峰,何军琴,等.补肾活血方案治疗排卵障碍性不孕不育的多中心随机双盲对照临床研究[J].中国中药杂志,2015,40(20):3911-3915.
[14]金炫廷,马堃,单婧.补肾活血中药治疗多囊卵巢综合征导致排卵障碍性不孕的临床研究[J].中国中药杂志,2014,39(1):140-143.
[15]高秀玲,刘微微.补肾调周法治疗黄体功能不全性不孕症的临床观察[J].四川中医,2012,32(12):94-96.
[16]郑纯,余娜,吕德欣.补肾调肝活血方治疗卵巢早衰117例临床观察[J].湖南中医杂志,2012,28(2):17-19.
[17]武志娟,刘瑞芬.刘瑞芬辨治输卵管性不孕经验[J].辽宁中医杂志,2013,40(6):1088-1089.
[18]张琼,周一辰,孔珏莹.补肾活血通络方联合宫腹腔镜通液术治疗血瘀型输卵管性不孕的临床观察[J].上海中医药杂志,2016,50(6):64-66.
[19]王双双,胡静文,孙慧霞.活血通络方配合子宫输卵管通液术治疗输卵管性不孕的临床观察[J].中国实验方剂学杂志,2014,20(4):167-170.
[20]汤伟伟,马小平,朱利,等.补肾活血汤对子宫内膜异位症相关性不孕患者子宫内膜容受性的影响[J].中国医药导报,2016,13(25):122-124.
[21]具春花,陈玲.补肾活血方对内异症伴不孕患者子宫内膜的影响[J].辽宁中医杂志,2008,35(5):722-723.
[22]张玉蓉,徐莉,谢波.补肾活血法对子宫内膜异位症不孕患者EMAB、CA125的影响[J].中华中医药杂志,2004,19(5):319-320.
[23]司徒仪,徐莉.补肾活血法对子宫内膜异位症不孕患者外周血TNF、SOD影响[J].上海中医药杂志,2006,40(3):36-37.
[24]刘慧聪,刘敛,徐莲薇,等.李祥云从肾虚血瘀辨治免疫性不孕经验[J].上海中医药杂志,2013,47(12):13-15.
[25]宋淑华.中药补肾活血法治疗免疫性不孕32例[J].辽宁中医药大学学报,2007,9(4):95-95.
[26]蔡仁燕,詹新林,冯宗文.活血消抗汤治疗抗子宫内膜抗体阳性免疫性不孕40例[J].中医杂志,2010,51(7):627-628.
[27]EISENBERG E. Long-term outcomes in children born after assisted conception[J]. Semin Reprod Med,2012,30(2):123-130.
[28]李东.补肾养血活血法联合体外受精-胚胎移植对妊娠率的影响[J].中医杂志,2008,49(12):1084-1086.
[29]张宁.补肾活血法对IVF周期PCOS患者卵泡膜血流的影响[J].现代中西医结合杂志,2011,20(29):3641-3643.
[30]朱爱萍,张敏,张建平,等.补肾调经方对输卵管性不孕症患者体外受精—胚胎移植超排卵周期GDF-9的影响[J].中国中西医结合杂志,2011,31(6):780-783.
[31]宋俏蔚,陈嫣,孙亦婷,等.补肾活血调周法改善反复体外受精-胚胎移植失败患者卵巢储备功能的机理研究[J].中华中医药学刊,2014,32(2):375-377.
[32]周璐,徐彩华,孙一红.补肾活血调周法改善反复体外受精-胚胎移植失败患者子宫内膜容受性临床观察[J].中华中医药学刊,2013,31(1):154-156.
[33]周小秀,章巧萍,王秀春,等.补肾清肝活血汤对多囊卵巢综合征大鼠血清性激素水平的影响[J].浙江中医药大学学报,2012,36(7):792-794.
[34]胡亮亮,胡平,黄光荣,等.调经活血汤对多囊卵巢大鼠卵巢颗粒细胞凋亡的影响及机制研究[J].中国中医急症,2009,18(2):261-263.
[35]胡兴文,王维鹏,洪媛,等.补肾活血方对小鼠卵泡刺激素及其受体mRNA表达的影响[J].中国免疫学杂志,2010,26(11):1003-1006.
[36]潘宇娜,李晓红,黄小芬.卵巢早衰大鼠应用补肾活血方对其免疫学指标及凋亡细胞的影响分析[J].中国妇幼保健,2015,30(2):299-301.
[37]HUANG C,SONG K,MA W,et al. Immunomodulatory mechanism of BushenHuoxue Recipe alleviates cyclophosphamide-induced diminished ovarian reserve in mouse model[J]. J Ethnopharmacol,2017,208(1):44-56.
[38]杨芳,杨健之.胰岛素、雄激素、胰岛素样生长因子、血管内皮生长因子对多囊卵巢综合征患者卵泡发育的影响[J].生殖与避孕,2013,33(6):413-417.
[39]胡晓玲,周青,彭朝龙,等.多囊卵巢综合征卵巢组织与血清中VEGF、ES及MVD的表达与意义[J].同济大学学报(医学版),2011,32(4):40-42.
[40]郑建淮,艾红,曹缵孙,等.多囊卵巢综合征患者肾素-血管紧张素与子宫卵巢血流动力学的相关性[J].西安交通大学学报(医学版),2004,25(5):478-480.
[41]王瑞杰,李晖,邱方,等.补肾活血化痰汤对多囊卵巢综合征模型大鼠卵巢血供及形态的影响[J].中国药房,2017,28(4):479-482.
[42]谈珍瑜,冯光荣,尤昭玲,等.恒河猴PCO模型卵巢VEGF mRNA的表达及中药补肾活血方对其影响的实验研究[J].中华中医药学刊,2007,25(4):786-788.
[43]BUKULMEZ O,ARICI A. Leukocytes in ovarian function[J]. Hum Reprod Update,2000,6(1):1-15.
[44]陈欣,宋岩,邓永志,等.补肾活血方对多囊卵巢综合征大鼠模型炎症因子的作用[J].中国妇幼保健,2016,31(21):4548-4549.
[45]王玉修.炎症因子IL-18与多囊卵巢综合征相关性研究[J].中国医师杂志,2013,15(10):1401-1403.
[46]WANG C,ROY S K. Expression of growth differentiation factor-9 in the oocytes is essential for the development of primordial follicles in the hamster ovary[J]. Endocrinology,2006,147(4):1725-1734.
[47]李爽,夏阳,夏天.补肾调冲方对半乳糖致POF大鼠始基卵泡募集及卵泡闭锁相关因子GDF-9表达的影响[J].辽宁中医杂志,2011,38(11):2267-2270.
[48]RILEY S C,LEASK R,CHARD T,et al. Secretion of matrix metalloproteinase-2,matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases into the intrauterine compartments during early pregnancy[J]. Mol Hum Reprod,1999,5(4):376-381.
[49]付灵梅,尤昭玲,罗岚.补肾活血方对多囊卵巢大鼠卵巢MMP-2、9的表达干预研究[J].湖南中医药大学学报,2008,28(6):29-31.
[50]王瑶,冯云.胞饮突—极具价值的种植窗预测工具[J].国际生殖健康/计划生育杂志,2006,25(2):77-80.
[51]QUINN C E,CASPER R F. Pinopodes:a questionable role in endometrial receptivity[J]. Hum Reprod Update,2009,15(2):229-236.
[52]AGHAJANOVA L,STAVREUS-EVERS A,NIKAS Y,et al. Coexpression of pinopodes and leukemia inhibitory factor,as well as its receptor,in human endometrium[J]. Fertil Steril,2003,79(S1):808.
[53]黄冬梅,黄光英,陆付耳.补肾益气和血方中药对胚泡着床障碍小鼠子宫内膜表面胞饮突表达的影响[J].中华妇产科杂志,2004,39(4):264.
[54]张琦,张勤华,贾亚楠.温肾活血方改善胚胎着床障碍小鼠子宫内膜容受性的研究[J].生殖与避孕,2016,36(11):875-881.
[55]李海霞,夏正明,张金玉,等.益气血补肝肾方对胚胎着床障碍小鼠子宫内膜整合素β3和胞饮突表达的影响[J].广州中医药大学学报,2016,33(5):688-692.
[56]DAFTARY G S,TROY P J,BAGOT C N. Direct regulation of beta3-integrin subunit gene expression by HOXA10 in endometrial cells[J]. Mol Endocrinol,2002,16(3):571.
[57]王晓慧,姜国华,刘宏,等.补肾活血方对小鼠HOXA10基因表达水平的影响[J].中医药信息,2016,33(4):49-51.
[58]张明敏,黄玉琴,程亮亮,等.补肾安胎方对胚泡着床障碍小鼠子宫内膜HB-EGF及其受体EGFR表达的影响[J].华中科技大学学报(医学版),2008,37(1):85-88.
[59]RASHID N A,LALITKUMAR S,LALITKUMAR P G,et al. Endometrial receptivity and human embryo implantation[J]. Am J Reprod Immunol,2011,66(S1):23-30.
[60]肖承悰,刘雁峰,江媚.“补肾气,通胞脉”改善子宫内膜容受性[J].生殖与避孕,2014,34(7):595-598.
[61]张明敏,何新芳,张锦金.补肾安胎方及其不同组分对胚泡着床障碍小鼠雌、孕激素及其受体的影响[J].中国中西医结合杂志,2010,30(3):291-294.
[62]NIKAS G,DRAKAKIS P,LOUTRADIS D,et al. Uterine pinopodes as markers of the"nidation window"in cycling women receiving exogenous oestradiol and progesterone[J]. Hum Reprod,1995,10(5):1208-1213.
[63]CHEN G,XIN A,LIU Y,et al. Integrinsβ1 andβ3 are biomarkers of uterine condition for embryo transfer[J]. J Transl Med,2016,14(1):303.
[64]余楠,杨菁,徐望明,等.助孕方对着床障碍模型小鼠胞饮突及白血病抑制因子表达的影响[J].中华临床医师杂志(电子版),2013,7(11):4873-4877.
[65]闫文杰,杨菁,尹太郎,等.中药助孕方对促排卵和胚泡着床障碍小鼠子宫内膜容受性的影响[J].中国中西医结合杂志,2012,32(11):1554-1557.
[66]孟艳岑,张明敏,崔丹丹.补肾、活血对超促排卵小鼠着床期间子宫内膜MMP-2、MMP-9、TIMP-3表达的影响[J].华中科技大学学报(医学版),2013,42(6):627-632.
[67]龚萍,黄光英.健胎液对胚胎着床障碍小鼠子宫炎性细胞因子表达的影响[J].中医杂志,2014,55(10):871-874.
[68]HUANG D M,HUANG G Y,LU F E. Effects of Bushenyiqihexue formula on the endometrial gland apoptosis in mice with blastocyst implantation dysfunction[J]. J Tradit Chin Med,2010,30(3):196-200.
[2]周猷,庞成,何电,等.我国不孕症研究进展[J].中国计划生育学杂志,2011,19(7):445-446.
[3]马丽亚,李宾玲,张大伟.《傅青主女科》从肾论治不孕症探析[J].中医学报,2015,30(12):1801-1803.
[4]马堃,佟雅婧.从肾虚血瘀论治排卵障碍性不孕[J].中国中药杂志,2017,42(23):4451-4454.
[5]丁键红,叶平.浅析古代医家对不孕症病因病机的认识[J].吉林中医药,2010,30(8):645-646.
[6]郝庆秀,王继峰,牛建昭,等.四物汤及组方中药植物雌激素活性的实验研究[J].中华中医药学刊,2009,27(4):738-741.
[7]秦达念,佘白蓉,佘运初.菟丝子黄酮对实验动物及人绒毛组织生殖功能的影响[J].中药新药与临床药理,2000,11(6):349-351.
[8]韦妍妍,张紫佳,徐颖,等.补骨脂对去卵巢大鼠雌激素样作用研究[J].中国实验方剂学杂志,2011,17(13):158-161.
[9]李艳,于涛,苗明三.淫羊藿的化学、药理与临床应用分析[J].中医学报,2017,32(4):619-622.
[10]高玉桂,王灵芝,唐冀雪.丹参酮的性激素样活性[J].中国医学科学院学报,1980,2(3):189-191.
[11]赵丕文,王大伟,牛建昭,等.红花等10种中药的植物雌激素活性研究[J].中国中药杂志,2007,32(5):436-439.
[12]王艳,周广举,严宗逊,等.川牛膝在去卵巢大鼠体内的雌激素效应及对脂代谢的影响[J].中国妇幼保健,2015,30(29):5063-5066.
[13]马堃,刘雁峰,何军琴,等.补肾活血方案治疗排卵障碍性不孕不育的多中心随机双盲对照临床研究[J].中国中药杂志,2015,40(20):3911-3915.
[14]金炫廷,马堃,单婧.补肾活血中药治疗多囊卵巢综合征导致排卵障碍性不孕的临床研究[J].中国中药杂志,2014,39(1):140-143.
[15]高秀玲,刘微微.补肾调周法治疗黄体功能不全性不孕症的临床观察[J].四川中医,2012,32(12):94-96.
[16]郑纯,余娜,吕德欣.补肾调肝活血方治疗卵巢早衰117例临床观察[J].湖南中医杂志,2012,28(2):17-19.
[17]武志娟,刘瑞芬.刘瑞芬辨治输卵管性不孕经验[J].辽宁中医杂志,2013,40(6):1088-1089.
[18]张琼,周一辰,孔珏莹.补肾活血通络方联合宫腹腔镜通液术治疗血瘀型输卵管性不孕的临床观察[J].上海中医药杂志,2016,50(6):64-66.
[19]王双双,胡静文,孙慧霞.活血通络方配合子宫输卵管通液术治疗输卵管性不孕的临床观察[J].中国实验方剂学杂志,2014,20(4):167-170.
[20]汤伟伟,马小平,朱利,等.补肾活血汤对子宫内膜异位症相关性不孕患者子宫内膜容受性的影响[J].中国医药导报,2016,13(25):122-124.
[21]具春花,陈玲.补肾活血方对内异症伴不孕患者子宫内膜的影响[J].辽宁中医杂志,2008,35(5):722-723.
[22]张玉蓉,徐莉,谢波.补肾活血法对子宫内膜异位症不孕患者EMAB、CA125的影响[J].中华中医药杂志,2004,19(5):319-320.
[23]司徒仪,徐莉.补肾活血法对子宫内膜异位症不孕患者外周血TNF、SOD影响[J].上海中医药杂志,2006,40(3):36-37.
[24]刘慧聪,刘敛,徐莲薇,等.李祥云从肾虚血瘀辨治免疫性不孕经验[J].上海中医药杂志,2013,47(12):13-15.
[25]宋淑华.中药补肾活血法治疗免疫性不孕32例[J].辽宁中医药大学学报,2007,9(4):95-95.
[26]蔡仁燕,詹新林,冯宗文.活血消抗汤治疗抗子宫内膜抗体阳性免疫性不孕40例[J].中医杂志,2010,51(7):627-628.
[27]EISENBERG E. Long-term outcomes in children born after assisted conception[J]. Semin Reprod Med,2012,30(2):123-130.
[28]李东.补肾养血活血法联合体外受精-胚胎移植对妊娠率的影响[J].中医杂志,2008,49(12):1084-1086.
[29]张宁.补肾活血法对IVF周期PCOS患者卵泡膜血流的影响[J].现代中西医结合杂志,2011,20(29):3641-3643.
[30]朱爱萍,张敏,张建平,等.补肾调经方对输卵管性不孕症患者体外受精—胚胎移植超排卵周期GDF-9的影响[J].中国中西医结合杂志,2011,31(6):780-783.
[31]宋俏蔚,陈嫣,孙亦婷,等.补肾活血调周法改善反复体外受精-胚胎移植失败患者卵巢储备功能的机理研究[J].中华中医药学刊,2014,32(2):375-377.
[32]周璐,徐彩华,孙一红.补肾活血调周法改善反复体外受精-胚胎移植失败患者子宫内膜容受性临床观察[J].中华中医药学刊,2013,31(1):154-156.
[33]周小秀,章巧萍,王秀春,等.补肾清肝活血汤对多囊卵巢综合征大鼠血清性激素水平的影响[J].浙江中医药大学学报,2012,36(7):792-794.
[34]胡亮亮,胡平,黄光荣,等.调经活血汤对多囊卵巢大鼠卵巢颗粒细胞凋亡的影响及机制研究[J].中国中医急症,2009,18(2):261-263.
[35]胡兴文,王维鹏,洪媛,等.补肾活血方对小鼠卵泡刺激素及其受体mRNA表达的影响[J].中国免疫学杂志,2010,26(11):1003-1006.
[36]潘宇娜,李晓红,黄小芬.卵巢早衰大鼠应用补肾活血方对其免疫学指标及凋亡细胞的影响分析[J].中国妇幼保健,2015,30(2):299-301.
[37]HUANG C,SONG K,MA W,et al. Immunomodulatory mechanism of BushenHuoxue Recipe alleviates cyclophosphamide-induced diminished ovarian reserve in mouse model[J]. J Ethnopharmacol,2017,208(1):44-56.
[38]杨芳,杨健之.胰岛素、雄激素、胰岛素样生长因子、血管内皮生长因子对多囊卵巢综合征患者卵泡发育的影响[J].生殖与避孕,2013,33(6):413-417.
[39]胡晓玲,周青,彭朝龙,等.多囊卵巢综合征卵巢组织与血清中VEGF、ES及MVD的表达与意义[J].同济大学学报(医学版),2011,32(4):40-42.
[40]郑建淮,艾红,曹缵孙,等.多囊卵巢综合征患者肾素-血管紧张素与子宫卵巢血流动力学的相关性[J].西安交通大学学报(医学版),2004,25(5):478-480.
[41]王瑞杰,李晖,邱方,等.补肾活血化痰汤对多囊卵巢综合征模型大鼠卵巢血供及形态的影响[J].中国药房,2017,28(4):479-482.
[42]谈珍瑜,冯光荣,尤昭玲,等.恒河猴PCO模型卵巢VEGF mRNA的表达及中药补肾活血方对其影响的实验研究[J].中华中医药学刊,2007,25(4):786-788.
[43]BUKULMEZ O,ARICI A. Leukocytes in ovarian function[J]. Hum Reprod Update,2000,6(1):1-15.
[44]陈欣,宋岩,邓永志,等.补肾活血方对多囊卵巢综合征大鼠模型炎症因子的作用[J].中国妇幼保健,2016,31(21):4548-4549.
[45]王玉修.炎症因子IL-18与多囊卵巢综合征相关性研究[J].中国医师杂志,2013,15(10):1401-1403.
[46]WANG C,ROY S K. Expression of growth differentiation factor-9 in the oocytes is essential for the development of primordial follicles in the hamster ovary[J]. Endocrinology,2006,147(4):1725-1734.
[47]李爽,夏阳,夏天.补肾调冲方对半乳糖致POF大鼠始基卵泡募集及卵泡闭锁相关因子GDF-9表达的影响[J].辽宁中医杂志,2011,38(11):2267-2270.
[48]RILEY S C,LEASK R,CHARD T,et al. Secretion of matrix metalloproteinase-2,matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases into the intrauterine compartments during early pregnancy[J]. Mol Hum Reprod,1999,5(4):376-381.
[49]付灵梅,尤昭玲,罗岚.补肾活血方对多囊卵巢大鼠卵巢MMP-2、9的表达干预研究[J].湖南中医药大学学报,2008,28(6):29-31.
[50]王瑶,冯云.胞饮突—极具价值的种植窗预测工具[J].国际生殖健康/计划生育杂志,2006,25(2):77-80.
[51]QUINN C E,CASPER R F. Pinopodes:a questionable role in endometrial receptivity[J]. Hum Reprod Update,2009,15(2):229-236.
[52]AGHAJANOVA L,STAVREUS-EVERS A,NIKAS Y,et al. Coexpression of pinopodes and leukemia inhibitory factor,as well as its receptor,in human endometrium[J]. Fertil Steril,2003,79(S1):808.
[53]黄冬梅,黄光英,陆付耳.补肾益气和血方中药对胚泡着床障碍小鼠子宫内膜表面胞饮突表达的影响[J].中华妇产科杂志,2004,39(4):264.
[54]张琦,张勤华,贾亚楠.温肾活血方改善胚胎着床障碍小鼠子宫内膜容受性的研究[J].生殖与避孕,2016,36(11):875-881.
[55]李海霞,夏正明,张金玉,等.益气血补肝肾方对胚胎着床障碍小鼠子宫内膜整合素β3和胞饮突表达的影响[J].广州中医药大学学报,2016,33(5):688-692.
[56]DAFTARY G S,TROY P J,BAGOT C N. Direct regulation of beta3-integrin subunit gene expression by HOXA10 in endometrial cells[J]. Mol Endocrinol,2002,16(3):571.
[57]王晓慧,姜国华,刘宏,等.补肾活血方对小鼠HOXA10基因表达水平的影响[J].中医药信息,2016,33(4):49-51.
[58]张明敏,黄玉琴,程亮亮,等.补肾安胎方对胚泡着床障碍小鼠子宫内膜HB-EGF及其受体EGFR表达的影响[J].华中科技大学学报(医学版),2008,37(1):85-88.
[59]RASHID N A,LALITKUMAR S,LALITKUMAR P G,et al. Endometrial receptivity and human embryo implantation[J]. Am J Reprod Immunol,2011,66(S1):23-30.
[60]肖承悰,刘雁峰,江媚.“补肾气,通胞脉”改善子宫内膜容受性[J].生殖与避孕,2014,34(7):595-598.
[61]张明敏,何新芳,张锦金.补肾安胎方及其不同组分对胚泡着床障碍小鼠雌、孕激素及其受体的影响[J].中国中西医结合杂志,2010,30(3):291-294.
[62]NIKAS G,DRAKAKIS P,LOUTRADIS D,et al. Uterine pinopodes as markers of the"nidation window"in cycling women receiving exogenous oestradiol and progesterone[J]. Hum Reprod,1995,10(5):1208-1213.
[63]CHEN G,XIN A,LIU Y,et al. Integrinsβ1 andβ3 are biomarkers of uterine condition for embryo transfer[J]. J Transl Med,2016,14(1):303.
[64]余楠,杨菁,徐望明,等.助孕方对着床障碍模型小鼠胞饮突及白血病抑制因子表达的影响[J].中华临床医师杂志(电子版),2013,7(11):4873-4877.
[65]闫文杰,杨菁,尹太郎,等.中药助孕方对促排卵和胚泡着床障碍小鼠子宫内膜容受性的影响[J].中国中西医结合杂志,2012,32(11):1554-1557.
[66]孟艳岑,张明敏,崔丹丹.补肾、活血对超促排卵小鼠着床期间子宫内膜MMP-2、MMP-9、TIMP-3表达的影响[J].华中科技大学学报(医学版),2013,42(6):627-632.
[67]龚萍,黄光英.健胎液对胚胎着床障碍小鼠子宫炎性细胞因子表达的影响[J].中医杂志,2014,55(10):871-874.
[68]HUANG D M,HUANG G Y,LU F E. Effects of Bushenyiqihexue formula on the endometrial gland apoptosis in mice with blastocyst implantation dysfunction[J]. J Tradit Chin Med,2010,30(3):196-200.