宏基因组学技术在水产动物病毒鉴定中的应用
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摘要
近年来水产养殖生产中病毒性疾病的发生越来越严重,所以对水产动物病毒的深入研究成为水产研究者的一个重要课题。随着宏基因组学技术的不断发展,以及测序成本的快速下降,基于宏基因组学技术的水产动物病毒鉴定技术逐渐成为水产动物病毒鉴定的主要技术之一。本文总结了基于宏基因组学的水产动物病毒鉴定技术的主要方法,详细综述了鉴定技术的实现流程,评述了几种基于宏基因组学的水产动物病毒鉴定技术的优缺点,并对基于宏基因组学的水产动物病毒鉴定技术的未来发展方向进行了展望。
In recent years,the occurrence of viral diseases in the production of aquaculture has become more and more serious. Therefore,in-depth research on aquatic virus has become an important issue for aquatic researchers. With the continuous development of metagenomics technology and the rapid decline in sequencing costs,aquatic animal virus identification technology based on metagenomics technology has gradually become one of the main techniques for identification of aquatic animal viruses. This article summarized the main methods for the identification of aquatic animal viruses based on metagenomics,reviews the identification process in detail,and discussed the advantages and disadvantages of the identification techniques. The development of the identification technology of aquatic animal viruses has been forecasted.
In recent years,the occurrence of viral diseases in the production of aquaculture has become more and more serious. Therefore,in-depth research on aquatic virus has become an important issue for aquatic researchers. With the continuous development of metagenomics technology and the rapid decline in sequencing costs,aquatic animal virus identification technology based on metagenomics technology has gradually become one of the main techniques for identification of aquatic animal viruses. This article summarized the main methods for the identification of aquatic animal viruses based on metagenomics,reviews the identification process in detail,and discussed the advantages and disadvantages of the identification techniques. The development of the identification technology of aquatic animal viruses has been forecasted.
引文
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[17]何彪,涂长春.病毒宏基因组学的研究现状及应用[J].畜牧兽医学报,2012,43(12):1865-1870.
[18] SHENDURE J,JI H. Next-generation DNA sequencing[J]. Na-ture Biotechnology,2008,26(10):1135-1145.
[19] DAY J M,BALLARD L L,DUKE M V,et al. Metagenomic anal-ysis of the turkey gut RNA virus community[J]. Virology Jour-nal,2010,7:313.
[20] EMMENEGGER E J,GLENN J A,WINTON J R,et al. Molecu-lar identification of erythrocytic necrosis virus(ENV)from theblood of Pacific herring(Clupea pallasii)[J]. Veterinary Micro-biology,2014,174(1/2):16-26.
[21] HONKAVUORI K S,BRIESE T,KRAUSS S,et al. Novel coro-navirus and astrovirus in delaware bay shorebirds[J]. PLoSONE,2014,9(4):e93395.
[22] PHAN T G,VO N P,ARONEN M,et al. Novel human gamma-papillomavirus species in a nasal swab[J]. Genome Announc,2013,1(2):e0002213.
[23] QUAN P-L,FIRTH C,CONTE J M,et al. Bats are a major natu-ral reservoir for hepaciviruses and pegiviruses[J]. Proceedings ofthe National Academy of Sciences of the United States of America,2013,110(20):8194-8199.
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