黄酒对健康大鼠肥胖相关指标的影响
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摘要
研究黄酒对健康大鼠肥胖指标体质量、血脂及肠道菌群的影响。24只健康雄性SD大鼠,随机分为黄酒组、酒基对照组和空白对照组,分别给予黄酒、15%酒基和蒸馏水,连续灌胃35 d后,测定体质量增量、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量及肠道菌群多样性。结果表明,黄酒对大鼠体质量增加及血脂指标没有显著影响(P≥0.05)。高通量测序结果显示,黄酒能够上调拟杆菌门并下调厚壁菌门组成比例;能够上调双歧杆菌属(Bifidobacterium)、嗜粘蛋白-艾克曼菌属(Akkermansia muciphila)和拟杆菌属(Bacteroides)组成比例。物种α-多样性分析结果显示,黄酒对大鼠肠道菌群多样性没有显著影响(P≥0.05)。在实验周期内,黄酒有抑制大鼠体质量增加的趋势,可能与能够调节肥胖有关的肠道菌群比例有关。
The effects of Huangjiu(Chinese rice wine) on body mass, blood lipid and gut microbiota of healthy rats were studied. 24 healthy male SD rats were randomly divided into Huangjiu group, alcohol control group and blank control group. They were given Huangjiu, 15% alcohol and distilled water, respectively. After continuous gavage for 35 d, the body mass increment, total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C) contents and gut microbiota diversity were measured. The results showed that there was no significant difference on body mass and blood lipid comparing with the blank control group(P≥0.05). High-throughput sequencing of gut microbiota results showed that Huangjiu could up-regulate the composition ratio of Bacteroidetes, down-regulate the composition ratio of Firmicutes, and up-regulate the composition ratio of Bifidobacterium, Akkermansia muciphila and Bacteroides. The species alpha diversity analysis results showed that Huangjiu had no significant effect on gut microbiota diversity of rats(P≥0.05). During the experiment, Huangjiu had a tendency to inhibit the increase of body mass in rats, which may be related to the regulation of the proportion of gut microbiota related to obesity.
The effects of Huangjiu(Chinese rice wine) on body mass, blood lipid and gut microbiota of healthy rats were studied. 24 healthy male SD rats were randomly divided into Huangjiu group, alcohol control group and blank control group. They were given Huangjiu, 15% alcohol and distilled water, respectively. After continuous gavage for 35 d, the body mass increment, total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C) contents and gut microbiota diversity were measured. The results showed that there was no significant difference on body mass and blood lipid comparing with the blank control group(P≥0.05). High-throughput sequencing of gut microbiota results showed that Huangjiu could up-regulate the composition ratio of Bacteroidetes, down-regulate the composition ratio of Firmicutes, and up-regulate the composition ratio of Bifidobacterium, Akkermansia muciphila and Bacteroides. The species alpha diversity analysis results showed that Huangjiu had no significant effect on gut microbiota diversity of rats(P≥0.05). During the experiment, Huangjiu had a tendency to inhibit the increase of body mass in rats, which may be related to the regulation of the proportion of gut microbiota related to obesity.
引文
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[16]LIU G R,PAN W H,CHANG X Y,et al.In vitro adherence properties of Bifidobacterium strains and their antagonistic activity against enteropathogens[J].Microbiol China,2009,36(5):716-721.
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[20]DERRIEN M,BELZER C,VOS W M D.Akkermansia muciniphila,and its role in regulating host functions[J].Microb Pathog,2017,106:171-181.
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[2]全国食品工业标准化技术委员会酿酒分技术委员会.GB/T 13662-2008黄酒[S].北京:中国标准出版社,2009.
[3]徐岳正.黄酒健康功能的研究现状与发展[J].中国酒,2018(4):52-57.
[4]刘月,祁国栋,张炳文.黄酒功能成分的研究进展[J].中国酿造,2015,34(11):27-30.
[5]谢广发,朱成刚,胡志明,等.黄酒对大鼠体重影响的初步研究[J].酿酒,2011(38):47-48.
[6]王丽媛,秦文,李岩,等.黄酒对便秘模型小鼠通便及肠道菌群的影响[J].中国酿造,2018,37(11):36-39.
[7]HILDEBRAND F,NGUYEN T L A,BRINKMAN B,et al.Inflammationassociated enterotypes,host geno-type,cage and inter-individual effects drive gut microbiota variation in common laboratory mice[J].Genome Biol,2013,14(1):R4.
[8]SCHLOSS P D,WESTCOTT S L,RYABIN T,et al.Introducing mother:open-source,platform-independent,community-supported software for describing and comparing microbial communities[J].Appl Environ Microbiol,2009,75(23):7537-7541.
[9]高洁,孙静,黄建,等.开菲尔对高脂金黄地鼠肠道菌群的调节作用研究[J].中国酿造,2017,36(4):15-19.
[10]LEY R E,BACKHED F,TURNBAUGH P,et al.Obesity alters gut microbial ecology[J].P Natl Acad Sci USA,2005,102(31):11070-11075.
[11]TURNBAUGH P J,LEY R E,MAHOWALD M A,et al.The gut microbiome as a biomarker and therapeutic target for treating obesity or an obesity related disorder:US,EP2102350[P].2009-09-23.
[12]SERINO M,LUCHE E,GRES S,et al.Metabolic adaptation to a highfat diet is associated with a change in the gut microbiota[J].Gut,2012,61(4):543-553.
[13]XIE W,GU D,LI J,et al.Effects and action mechanisms of berberine and rhizoma coptidis on gut microbes and obesity in high-fat diet-fed C57BL/6J mice[J].PLoS One,2011,6(9):e24520.
[14]LEY R E,TURNBAUGH P J,KLEIN S,et al.Human gut microbes associated with obesity[J].Nature,2006,444(21):1022-1023.
[15]郭婉.清爽型银耳黄酒酿造关键工艺及其体外抗氧化研究[D].福州:福建农林大学,2014.
[16]LIU G R,PAN W H,CHANG X Y,et al.In vitro adherence properties of Bifidobacterium strains and their antagonistic activity against enteropathogens[J].Microbiol China,2009,36(5):716-721.
[17]YUSOF R M,HAQUE F,ISMAIL M,et al.Isolation of Bifidobacteria infantis and its antagonistic activity against ETEC 0157 and Salmonella typhimurium S-285 in weaning foods[J].Asia Pac J Clin Nutr,2000,9(2):130-135.
[18]DAO M C,EVERARD A,ARONWISNEWSKY J,et al.Akkermansia muciniphila and improved metabolic health during a dietary intervention in obesity:relationship with gut microbiome richness and ecology[J].Gut,2016,65(3):426-436.
[19]REMELY M,HIPPE B,ZANNER J,et al.Gut microbiota of obese,type2 diabetic individuals is enriched in Faecalibacterium prausnitzii,Akkermansia muciniphila and Peptostreptococcus anacrobius after weight loss[J].Endocr Metab Immune Disord Drug Targets,2015,15(1):1-8.
[20]DERRIEN M,BELZER C,VOS W M D.Akkermansia muciniphila,and its role in regulating host functions[J].Microb Pathog,2017,106:171-181.
[21]冯淑贞,张和平.脆弱拟杆菌的研究进展[J].微生物学通报,2015,42(7):1366-1371.
[22]曾艳华,陈玛宁,张宁,等.大蒜多糖对人体肠道中拟杆菌生长的影响[J].现代食品科技,2010,26(5):445-447.