骨碎补总黄酮对大鼠胫骨牵张末期BMP-2与Smad-1表达的影响
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摘要
目的:探讨骨碎补总黄酮干预下BMP-2与Smad-1在大鼠胫骨牵张成骨末期中的表达。方法:40只大鼠建立大鼠胫骨牵张成骨模型,随机分成4组,分别为对照组、中药组、阻遏剂组及阻遏剂加中药组,每组10只。所有大鼠于牵张第20天时给予拍片评估,处死,取标本,HE染色评价成骨质量,用免疫组化分析各组BMP-2与Smad-1的表达。结果:中药组BMP-2与Smad-1的表达最高(P<0.05),阻遏剂加中药组明显高于阻遏剂组(P<0.05)。结论:BMP-2与Smad-1的表达在牵张成骨期间对成骨有重要促进作用,补肾法可以促进大鼠胫骨牵张末期BMP-2与Smad-1的表达。
Objective: To explore the expressions of BMP-2 and Smad-1 in rat tibia which were treated by drynaria rhizome during distraction osteogenesis. Methods: Forty rat tibia distraction osteogenesis models were constructed,then were randomly assigned into four groups,which were control group,Chinese medicines group,Suppressor group and Suppressor adding Chinese medicine group,there were ten animals in each group. All animals were examined with X-Ray on 20 th day since distraction,then were sacrificed for tissues sampling and trimming. HE stanning was conducted to evaluate osteogenesis quality,and Immunohistochemistry was used to estimate the expressions of BMP-2 and Smad-1. Results: The expression levels of BMP-2 and Smad-1 were the highest in Chinese medicine group(P < 0. 05),and which were higher in Suppressor adding Chinese medicine group than that in Suppressor group. Conclusion: The expressions of BMP-2 and Smad-1 have important facilitation on osteogenesis during distraction osteogenesis,and the expressions of BMP-2 and Smad-1 during the last stage of distraction osteogenesis could be promoted by tonifying kidney method.
Objective: To explore the expressions of BMP-2 and Smad-1 in rat tibia which were treated by drynaria rhizome during distraction osteogenesis. Methods: Forty rat tibia distraction osteogenesis models were constructed,then were randomly assigned into four groups,which were control group,Chinese medicines group,Suppressor group and Suppressor adding Chinese medicine group,there were ten animals in each group. All animals were examined with X-Ray on 20 th day since distraction,then were sacrificed for tissues sampling and trimming. HE stanning was conducted to evaluate osteogenesis quality,and Immunohistochemistry was used to estimate the expressions of BMP-2 and Smad-1. Results: The expression levels of BMP-2 and Smad-1 were the highest in Chinese medicine group(P < 0. 05),and which were higher in Suppressor adding Chinese medicine group than that in Suppressor group. Conclusion: The expressions of BMP-2 and Smad-1 have important facilitation on osteogenesis during distraction osteogenesis,and the expressions of BMP-2 and Smad-1 during the last stage of distraction osteogenesis could be promoted by tonifying kidney method.
引文
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[16]张军,李浩鹏,杨平林,等.骨碎补总黄酮含药血清对成骨细胞增殖、分化、周期及凋亡的影响[J].中药材,2009,32(7):1090-1093.
[2]曾景奇,黄枫,姜自伟,等.一种牵张成骨大鼠实验模型的建立[J].中国实验动物学报,2016,24(1):43-36.
[3]孙丙银.骨碎补总黄酮促进股骨缺损牵张成骨新骨形成的实验研究[D].广州:广州中医药大学,2013:43-44.
[4]孙溪饶.牵引速率及频率对牵张成骨的影响[J].中国组织工程研究与临床康复,2010,14(41),7727-7730.
[5]秦泗河,李刚.Ilizarov理论与技术的起源、发展与传播史[J].中国骨与关节外科,2010,3(5):417-423.
[6]Ashman O,Phillips AM.Treatment of non-unions with bone defects:which option andwhy?[J].Injury,2013,44(Suppl1):43-45.
[7]Abe E.Function of BMPs and BMP antagonists in adult bone[J].Ann N Y Acad Sci,2006,1068(1):41-53.
[8]Rosen V.BMP and BMP inhibitors in bone[J].Ann N Y Acad Sci,2006,1068(1):19-25.
[9]Rengachary SS.Bone morphogenetic proteins:basic concepts[J].Neurosurg Focus,2002,13(6):1-6.
[10]Haque T,Hamade F,Alam N,et al.Characterizing the BMP pathway in a wild type mouse model of distraction osteogenesis[J].Bone,2008,42(6):1144-1153.
[11]Ashinoff R L,Cetrulo C L Jr,Galiano R D,et al.Bone Morphogentic protein-2 gene therapy for mandibular distraction osteogenesis J[J].Ann Plast Surg,2004,52(6):585.
[12]黄旋平,周诺,江献芳,等.人骨形态发生蛋白2基因修饰自体骨髓间充质干细胞移植促进兔下颌骨牵张成骨实验的组织形态学观察[J].中国组织工程研究,2012,16(23):4242.
[13]MASSAGUE J.TGF-beta signal transduction[J].Anna Rev Biochem,1998,67(1):753-791.
[14]Yoshimura Y,Nomura S,Kawasaki S,et al.Colocalization of noggin and bone morphogenetic protein-4 during fracture healing[J].J Bone Miner Res,2001,16(5):876-884.
[15]彭双,韩立峰,王涛,等.骨碎补中的化学成分及药理作用研究进展[J].天津中医药大学学报,2012,31(2):122-125.
[16]张军,李浩鹏,杨平林,等.骨碎补总黄酮含药血清对成骨细胞增殖、分化、周期及凋亡的影响[J].中药材,2009,32(7):1090-1093.