病毒转染技术在骨组织细胞研究中的应用
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摘要
随着病毒转染技术的发展,运用病毒转染骨组织细胞的方法来研究骨组织代谢及其相关的基因治疗已经越来越热门。本文总结了近年来运用病毒在体内外转染骨组织细胞的研究进展,为选择最合适的病毒载体及最佳的转染方式提供有价值的参考,为研究骨组织细胞的调控机制及代谢提供更有效的研究手段。
With the development of viral gene delivery techniques,much attention has been attracted on the therapy of bone metabolism diseases via bone cells transfection. In this review,recent progresses about viral vector transfection of bone cells have been summarized,which provides precious reference for the selection of the most appropriate technique of bone cells transfection. Furthermore,it can provide valuable research means for the illustration of the metabolic and regulatory mechanism behind the bone cells.
With the development of viral gene delivery techniques,much attention has been attracted on the therapy of bone metabolism diseases via bone cells transfection. In this review,recent progresses about viral vector transfection of bone cells have been summarized,which provides precious reference for the selection of the most appropriate technique of bone cells transfection. Furthermore,it can provide valuable research means for the illustration of the metabolic and regulatory mechanism behind the bone cells.
引文
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[2]张海峰,杜子婧,赵丹阳,等.绿色荧光蛋白基因转染示踪比格犬体内骨髓间充质干细胞分化[J].国际骨科学杂志,2016,37(2):117-122.
[3]姚雪雁,杨薇,李海波,等.重组慢病毒GV303 Rat IL-33体外转染原代大鼠骨髓间充质干细胞的研究[J].国际眼科杂志,2017,17(5):841-845.
[4]康健,侯洋,周许辉,等. TGF-β3和BMP-7腺病毒共转染兔骨髓间充质干细胞向类髓核细胞分化的研究[J].中国脊柱脊髓杂志,2014,24(2):157-163.
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[6] Hioki H,Kameda H,Nakamura H,et al. Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters[J]. Gene Ther,2007,14(11):872-882.
[7] Bakota L,Brandt R,Heinisch JJ. Triple mammalian/yeast/bacterial shuttle vectors for single and combined Lentivirus-and Sindbis virus-mediated infections of neurons[J]. Mol Genet Genomics,2012,287(4):313-324.
[8]张创强,孙柯,马珏,等.慢病毒介导的大鼠前强啡肽基因在骨髓间充质干细胞中的表达[J].医学研究生学报,2017,30(2):138-144.
[9]杨国曦,朱庆生,杨重飞. Mac-1在破骨细胞分化中的作用[[J].中国病理生理杂志,2017,33(3):539-542+547.
[10]王伟卓,程一钊,郭雄,等.大骨节病差异表达基因PAPSS2对成骨细胞矿化及碱性磷酸酶活性的影响[J].西安交通大学学报(医学版),2014,35(2):175-181.
[11] Hiltunen MO,Ruuskanen M,Huuskonen J,et al. Adenovirus-mediated VEGF-A gene transfer induces bone formation in vivo[J].FASEB J,2003,17(9):1147-1149.
[12] Guo Z,Hong S,Jin X,et al. Study on the multidrug resistance 1gene transfection efficiency using adenovirus vector enhanced by ultrasonic microbubbles in vitro[J]. Mol Biotechnol,2011,48(2):138-146.
[13]李志勇,刘磊,田卫东,等.绿色荧光蛋白基因转染大鼠骨髓间充质干细胞的实验研究[J].中华口腔医学杂志,2005,40(2):150-153.
[14] Lv C,Yang S,Chen X,et al. MicroRNA-21 promotes bone mesenchymal stem cells migration in vitro by activating PI3K/Akt/MMPs pathway[J]. J Clin Neurosci,2017,46:156-162.
[15] Lee YL,Lin SK,Hou KL,et al. Sirtuin 6 attenuates periapical lesion propagation by modulating hypoxia-induced chemokine(C-C motif)ligand 2 production in osteoblasts[J]. Int Endod J,2018,51:e74-e86.
[16]朱云,王正宇,杨小中,等.小鼠Maf B基因重组腺病毒载体的构建及其对破骨细胞分化的影响[J].吉林大学学报(医学版),2015,41(1):32-38,207-208.
[17] Royo NC,Vandenberghe LH,Ma JY,et al. Specific AAV serotypes stably transduce primary hippocampal and cortical cultures with high efficiency and low toxicity[J]. Brain Res,2008,1190:15-22.
[18]戚孟春,孙红,胡静,等.重组腺病毒-骨形成蛋白7对骨髓间充质干细胞骨向分化影响的实验观察[J].中华口腔医学杂志,2007,42(4):245-248.
[19] Yuan SH,Gao CB,Yin CU,et al. Recombinant adeno-associated virus BMP-4/7 fusion gene confers ossification activity in rabbit bone marrow stromal cells[J]. Genet Mol Res,2012,11(3):3105-3114.
[20]王美华,胡锴勋,李晓兵. KGF和EGFP双基因共表达的重组腺病毒转染小鼠骨髓间充质干细胞的研究[J].中国实验血液学杂志,2012,20(2):427-432.
[21]蒋星,杨昌永,毛棉,等.两种重组腺相关病毒介导增强型绿色荧光蛋白转染大鼠成骨细胞效率的体外研究[J].四川大学学报(医学版),2010,41(2):203-207.
[22]胡硕,熊玲静,余剑英,等.改良腺病毒AdF35-eGFP转染人及大鼠骨髓间充质干细胞的效率对比研究[J].中南大学学报(医学版),2010,35(9):983-989.
[23] Li GB,Lu GX. Gene delivery efficiency in bone marrow-derived dendritic cells:comparison of four methods and optimization for lentivirus transduction[J]. Mol Biotechnol,2009,43(3):250-256.
[24]李诗鹏,李强,石正松,等.基因重组腺病毒载体与慢病毒载体转染兔骨髓间充质干细胞的对比[J].中国组织工程研究,2017,21(09):1340-1345.
[25]赖建明,林建华,林文平,等.猪TGF-β_1基因重组慢病毒载体的构建及其在BMSCs中的表达[J].中国修复重建外科杂志,2012,26(7):849-854.
[26]申星,郭玲玲,熊国林,等. ECSOD体外转染恒河猴骨髓间充质干细胞的实验研究[J].中国实验血液学杂志,2013,21(1):188-192.
[27] Lin JQ,Lin CZ,Lin XZ,et al. Construction of a bicistronic recombinant adenoviral vector for human interleukin-10 and enhanced green fluorescent protein expression in bone marrow mesenchymal stem cells[J]. Chin Med J(Engl),2012,125(1):102-108.
[28]张宁坤,陈宇,高连如,等.腺相关病毒2/1型载体转染大鼠骨髓间充质干细胞的实验研究[J].中国实验血液学杂志,2009,17(3):679-684.
[29] Buning H,Perabo L,Coutelle O,et al. Recent developments in adeno-associated virus vector technology[J]. J Gene Med,2008,10(7):717-733.
[30] Washbourne P,Mcallister AK. Techniques for gene transfer into neurons[J]. Current Opinion in Neurobiology,2002,12(5):566-573.
[31] Flotte TR. Gene therapy progress and prospects:recombinant adeno-associated virus(r AAV)vectors[J]. Gene Ther,2004,11(10):805-810.
[32]邓樑钧,邱飞.降低重组腺相关病毒载体免疫反应的策略[J].病毒学报,2018,34(1):121-127.
[33]黄旋平,谢庆条,江献芳,等.慢病毒载体介导的基因治疗在骨缺损修复中的研究进展[J].中国矫形外科杂志,2014,22(14):1284-1287.
[34] Montini E,Cesana D,Schmidt M,et al. Hematopoietic stem cell gene transfer in a tumor-prone mouse model uncovers low genotoxicity of lentiviral vector integration[J]. Nat Biotechnol,2006,24(6):687-696.
[35] Wazen RM,Moffatt P,Zalzal SF,et al. Local gene transfer to calcified tissue cells using prolonged infusion of a lentiviral vector[J]. Gene Ther,2006,13(22):1595-1602.
[36] Baltzer AW,Whalen JD,Wooley P,et al. Gene therapy for osteoporosis:evaluation in a murine ovariectomy model[J]. Gene Ther,2001,8(23):1770-1776.
[37]杨民,党耕町,马庆军.携带半乳糖苷酶报告基因重组腺相关病毒体内转染骨组织的实验观察[J].中国组织工程研究与临床康复,2007,2007(19):3677-3680.
[38] Peng WX,Wang L. Adenovirus-mediated expression of BMP-2 and BFGF in bone marrow mesenchymal stem cells combined with demineralized bone matrix for repair of femoral head osteonecrosis in beagle dogs[J]. Cell Physiol Biochem,2017,43(4):1648-1662.
[39]王敏,程政,姚天华,等.人骨形态发生蛋白2基因治疗大鼠牙槽骨缺损的实验研究[J].陕西医学杂志,2010,39(2):131-133,139.
[40]王艳娇,马厚勋,李宝善,等.腺相关病毒介导的klotho基因表达对去势大鼠骨Runx2及MMP-13表达的影响[J].基础医学与临床,2012,32(05):487-492.
[41]孟凡彪,刘宝华,刘佐君.尾静脉移植高表达Aqp1基因的骨髓间充质干细胞对股骨骨折大鼠骨愈合的影响[J].山东医药,2017,57(48):12-14.
[42] Mautino MR. Lentiviral vectors for gene therapy of HIV-1 infection[J]. Curr Gene Ther,2002,2(1):23-43.
[43] Naldini L,Blomer U,Gallay P,et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector[J].Science,1996,272(5259):263-267.
[44] Robl JM,Wang Z,Kasinathan P,et al. Transgenic animal production and animal biotechnology[J]. Theriogenology,2007,67(1):127-133.
[45] Zhang D,Yong L,Min S,et al. Autocrine,not paracrine,interferon-gamma gene delivery enhances ex vivo antigen-specific cytotoxic T lymphocyte stimulation and killing[J]. J Biomed Biotechnol,2010,2010(3):270985.
[46] Le Bec C,Douar AM. Gene therapy progress and prospects—vectorology:design and production of expression cassettes in AAV vectors[J]. Gene Therapy,2006,13(10):805-813.
[47] Keeler AM,Elmallah MK,Flotte TR. Gene Therapy 2017:progress and future directions[J]. Clin Transl Sci,2017,10(4):242-248.
[48] Fukuda A,Hikita A,Wakeyama H,et al. Regulation of osteoclast apoptosis and motility by small GTPase binding protein Rac1[J]. J Bone Miner Res,2005,20(12):2245-2253.
[49] Kobayashi Y,Take I,Yamashita T,et al. Prostaglandin E2 receptors EP2 and EP4 are down-regulated during differentiation of mouse osteoclasts from their precursors[J]. J Biol Chem,2005,280(25):24035-24042.
[50] Zhu Y,Li D,Zhang K,et al. Novel synthesized nanofibrous scaffold efficiently delivered hBMP-2 encoded in adenoviral vector to promote bone regeneration[J]. J Biomed Nanotechnol,2017,13(4):437-446.
[2]张海峰,杜子婧,赵丹阳,等.绿色荧光蛋白基因转染示踪比格犬体内骨髓间充质干细胞分化[J].国际骨科学杂志,2016,37(2):117-122.
[3]姚雪雁,杨薇,李海波,等.重组慢病毒GV303 Rat IL-33体外转染原代大鼠骨髓间充质干细胞的研究[J].国际眼科杂志,2017,17(5):841-845.
[4]康健,侯洋,周许辉,等. TGF-β3和BMP-7腺病毒共转染兔骨髓间充质干细胞向类髓核细胞分化的研究[J].中国脊柱脊髓杂志,2014,24(2):157-163.
[5] Hu JJ,Sun C,Lan L,et al. Therapeutic effect of transplanting beta(2)m(-)/Thy1(+)bone marrow-derived hepatocyte stem cells transduced with lentiviral-mediated HGF gene into CCl(4)-injured rats[J]. J Gene Med,2010,12(3):244-254.
[6] Hioki H,Kameda H,Nakamura H,et al. Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters[J]. Gene Ther,2007,14(11):872-882.
[7] Bakota L,Brandt R,Heinisch JJ. Triple mammalian/yeast/bacterial shuttle vectors for single and combined Lentivirus-and Sindbis virus-mediated infections of neurons[J]. Mol Genet Genomics,2012,287(4):313-324.
[8]张创强,孙柯,马珏,等.慢病毒介导的大鼠前强啡肽基因在骨髓间充质干细胞中的表达[J].医学研究生学报,2017,30(2):138-144.
[9]杨国曦,朱庆生,杨重飞. Mac-1在破骨细胞分化中的作用[[J].中国病理生理杂志,2017,33(3):539-542+547.
[10]王伟卓,程一钊,郭雄,等.大骨节病差异表达基因PAPSS2对成骨细胞矿化及碱性磷酸酶活性的影响[J].西安交通大学学报(医学版),2014,35(2):175-181.
[11] Hiltunen MO,Ruuskanen M,Huuskonen J,et al. Adenovirus-mediated VEGF-A gene transfer induces bone formation in vivo[J].FASEB J,2003,17(9):1147-1149.
[12] Guo Z,Hong S,Jin X,et al. Study on the multidrug resistance 1gene transfection efficiency using adenovirus vector enhanced by ultrasonic microbubbles in vitro[J]. Mol Biotechnol,2011,48(2):138-146.
[13]李志勇,刘磊,田卫东,等.绿色荧光蛋白基因转染大鼠骨髓间充质干细胞的实验研究[J].中华口腔医学杂志,2005,40(2):150-153.
[14] Lv C,Yang S,Chen X,et al. MicroRNA-21 promotes bone mesenchymal stem cells migration in vitro by activating PI3K/Akt/MMPs pathway[J]. J Clin Neurosci,2017,46:156-162.
[15] Lee YL,Lin SK,Hou KL,et al. Sirtuin 6 attenuates periapical lesion propagation by modulating hypoxia-induced chemokine(C-C motif)ligand 2 production in osteoblasts[J]. Int Endod J,2018,51:e74-e86.
[16]朱云,王正宇,杨小中,等.小鼠Maf B基因重组腺病毒载体的构建及其对破骨细胞分化的影响[J].吉林大学学报(医学版),2015,41(1):32-38,207-208.
[17] Royo NC,Vandenberghe LH,Ma JY,et al. Specific AAV serotypes stably transduce primary hippocampal and cortical cultures with high efficiency and low toxicity[J]. Brain Res,2008,1190:15-22.
[18]戚孟春,孙红,胡静,等.重组腺病毒-骨形成蛋白7对骨髓间充质干细胞骨向分化影响的实验观察[J].中华口腔医学杂志,2007,42(4):245-248.
[19] Yuan SH,Gao CB,Yin CU,et al. Recombinant adeno-associated virus BMP-4/7 fusion gene confers ossification activity in rabbit bone marrow stromal cells[J]. Genet Mol Res,2012,11(3):3105-3114.
[20]王美华,胡锴勋,李晓兵. KGF和EGFP双基因共表达的重组腺病毒转染小鼠骨髓间充质干细胞的研究[J].中国实验血液学杂志,2012,20(2):427-432.
[21]蒋星,杨昌永,毛棉,等.两种重组腺相关病毒介导增强型绿色荧光蛋白转染大鼠成骨细胞效率的体外研究[J].四川大学学报(医学版),2010,41(2):203-207.
[22]胡硕,熊玲静,余剑英,等.改良腺病毒AdF35-eGFP转染人及大鼠骨髓间充质干细胞的效率对比研究[J].中南大学学报(医学版),2010,35(9):983-989.
[23] Li GB,Lu GX. Gene delivery efficiency in bone marrow-derived dendritic cells:comparison of four methods and optimization for lentivirus transduction[J]. Mol Biotechnol,2009,43(3):250-256.
[24]李诗鹏,李强,石正松,等.基因重组腺病毒载体与慢病毒载体转染兔骨髓间充质干细胞的对比[J].中国组织工程研究,2017,21(09):1340-1345.
[25]赖建明,林建华,林文平,等.猪TGF-β_1基因重组慢病毒载体的构建及其在BMSCs中的表达[J].中国修复重建外科杂志,2012,26(7):849-854.
[26]申星,郭玲玲,熊国林,等. ECSOD体外转染恒河猴骨髓间充质干细胞的实验研究[J].中国实验血液学杂志,2013,21(1):188-192.
[27] Lin JQ,Lin CZ,Lin XZ,et al. Construction of a bicistronic recombinant adenoviral vector for human interleukin-10 and enhanced green fluorescent protein expression in bone marrow mesenchymal stem cells[J]. Chin Med J(Engl),2012,125(1):102-108.
[28]张宁坤,陈宇,高连如,等.腺相关病毒2/1型载体转染大鼠骨髓间充质干细胞的实验研究[J].中国实验血液学杂志,2009,17(3):679-684.
[29] Buning H,Perabo L,Coutelle O,et al. Recent developments in adeno-associated virus vector technology[J]. J Gene Med,2008,10(7):717-733.
[30] Washbourne P,Mcallister AK. Techniques for gene transfer into neurons[J]. Current Opinion in Neurobiology,2002,12(5):566-573.
[31] Flotte TR. Gene therapy progress and prospects:recombinant adeno-associated virus(r AAV)vectors[J]. Gene Ther,2004,11(10):805-810.
[32]邓樑钧,邱飞.降低重组腺相关病毒载体免疫反应的策略[J].病毒学报,2018,34(1):121-127.
[33]黄旋平,谢庆条,江献芳,等.慢病毒载体介导的基因治疗在骨缺损修复中的研究进展[J].中国矫形外科杂志,2014,22(14):1284-1287.
[34] Montini E,Cesana D,Schmidt M,et al. Hematopoietic stem cell gene transfer in a tumor-prone mouse model uncovers low genotoxicity of lentiviral vector integration[J]. Nat Biotechnol,2006,24(6):687-696.
[35] Wazen RM,Moffatt P,Zalzal SF,et al. Local gene transfer to calcified tissue cells using prolonged infusion of a lentiviral vector[J]. Gene Ther,2006,13(22):1595-1602.
[36] Baltzer AW,Whalen JD,Wooley P,et al. Gene therapy for osteoporosis:evaluation in a murine ovariectomy model[J]. Gene Ther,2001,8(23):1770-1776.
[37]杨民,党耕町,马庆军.携带半乳糖苷酶报告基因重组腺相关病毒体内转染骨组织的实验观察[J].中国组织工程研究与临床康复,2007,2007(19):3677-3680.
[38] Peng WX,Wang L. Adenovirus-mediated expression of BMP-2 and BFGF in bone marrow mesenchymal stem cells combined with demineralized bone matrix for repair of femoral head osteonecrosis in beagle dogs[J]. Cell Physiol Biochem,2017,43(4):1648-1662.
[39]王敏,程政,姚天华,等.人骨形态发生蛋白2基因治疗大鼠牙槽骨缺损的实验研究[J].陕西医学杂志,2010,39(2):131-133,139.
[40]王艳娇,马厚勋,李宝善,等.腺相关病毒介导的klotho基因表达对去势大鼠骨Runx2及MMP-13表达的影响[J].基础医学与临床,2012,32(05):487-492.
[41]孟凡彪,刘宝华,刘佐君.尾静脉移植高表达Aqp1基因的骨髓间充质干细胞对股骨骨折大鼠骨愈合的影响[J].山东医药,2017,57(48):12-14.
[42] Mautino MR. Lentiviral vectors for gene therapy of HIV-1 infection[J]. Curr Gene Ther,2002,2(1):23-43.
[43] Naldini L,Blomer U,Gallay P,et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector[J].Science,1996,272(5259):263-267.
[44] Robl JM,Wang Z,Kasinathan P,et al. Transgenic animal production and animal biotechnology[J]. Theriogenology,2007,67(1):127-133.
[45] Zhang D,Yong L,Min S,et al. Autocrine,not paracrine,interferon-gamma gene delivery enhances ex vivo antigen-specific cytotoxic T lymphocyte stimulation and killing[J]. J Biomed Biotechnol,2010,2010(3):270985.
[46] Le Bec C,Douar AM. Gene therapy progress and prospects—vectorology:design and production of expression cassettes in AAV vectors[J]. Gene Therapy,2006,13(10):805-813.
[47] Keeler AM,Elmallah MK,Flotte TR. Gene Therapy 2017:progress and future directions[J]. Clin Transl Sci,2017,10(4):242-248.
[48] Fukuda A,Hikita A,Wakeyama H,et al. Regulation of osteoclast apoptosis and motility by small GTPase binding protein Rac1[J]. J Bone Miner Res,2005,20(12):2245-2253.
[49] Kobayashi Y,Take I,Yamashita T,et al. Prostaglandin E2 receptors EP2 and EP4 are down-regulated during differentiation of mouse osteoclasts from their precursors[J]. J Biol Chem,2005,280(25):24035-24042.
[50] Zhu Y,Li D,Zhang K,et al. Novel synthesized nanofibrous scaffold efficiently delivered hBMP-2 encoded in adenoviral vector to promote bone regeneration[J]. J Biomed Nanotechnol,2017,13(4):437-446.