3种新型生态净化材料的脱氮效率及机理分析
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摘要
通过野外现场实验,对比生物绳(B-R)、生物毡(B-B)和碳素纤维生态草(B-G) 3种新型生态净化材料在不同温度下的氮素净化能力,并通过高通量测序分析材料表面微生物群落结构,进而探讨其脱氮机理。结果表明:所选择的3种材料在不同温度下均能显著降低水体中氮素浓度,其净化能力由高到低依次为生物绳>生态草>生物毡。生物绳和生态草表面富集的生物量更多,且生态草(夏19. 07%,冬18. 64%)和生物绳(夏18. 87%,冬18. 16%)表面功能属的相对丰度要高于生物毡(夏12. 45%,冬15. 17%)。在材料表面起脱氮作用的功能属主要包括Nitrospira、Flavobacterium、Acidovorax、Pseudomonas,Hydrogenophaga等。
In this study,field experiments were conducted to compare the nitrogen purification ability of three new types of ecological purification materials—biological rope( B-R),biological blanket( B-B) and carbon fiber biological grass( B-G)under high and low temperature. The characteristics of microorganism community on the surface of the material were studied by high throughput sequencing,and the mechanism of nitrogen removal was discussed. The results showed that the three selected materials could significantly reduce the nitrogen in the water,and could maintain good purification performance at different temperature. The purification ability was as follows: B-R > B-G > B-B. B-R and B-G had more abundant biomass,and the relative abundance of B-G( summer 19. 07%,winter 18. 64%) and B-R( summer 18. 87%,winter 18. 16%) was higher than that of B-B( summer 12. 45%,winter 15. 17%). The functions genus for nitrogen removal on the surface of the materials mainly included Nitrospira,Flavobacterium,Acidovorax,Pseudomonas,Hydrogenophaga,et al.
In this study,field experiments were conducted to compare the nitrogen purification ability of three new types of ecological purification materials—biological rope( B-R),biological blanket( B-B) and carbon fiber biological grass( B-G)under high and low temperature. The characteristics of microorganism community on the surface of the material were studied by high throughput sequencing,and the mechanism of nitrogen removal was discussed. The results showed that the three selected materials could significantly reduce the nitrogen in the water,and could maintain good purification performance at different temperature. The purification ability was as follows: B-R > B-G > B-B. B-R and B-G had more abundant biomass,and the relative abundance of B-G( summer 19. 07%,winter 18. 64%) and B-R( summer 18. 87%,winter 18. 16%) was higher than that of B-B( summer 12. 45%,winter 15. 17%). The functions genus for nitrogen removal on the surface of the materials mainly included Nitrospira,Flavobacterium,Acidovorax,Pseudomonas,Hydrogenophaga,et al.
引文
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[20] Liao R H,Shen K,Li A M,et al. High-nitrate wastewater treatment in an expanded granular sludge bed reactor and microbial diversity using 454 pyrosequencing analysis[J]. Bioresource Technology,2013,134:190-197.
[21]孙广垠,安恩方,刘勇,等.不同生物膜载体净化微污染水挂膜及处理效果研究[J].给水排水,2017,43(6):48-53.
[2]吴睿,张晓松,戴江玉,等. 3种人工载体净化富营养化水体能力的比较[J].湖泊科学,2014,26(5):682-690.
[3] Calderon K,Martin-Pascual J,Poyatos J M,et al. Comparative analysis of the bacterial diversity in a lab-scale moving bed biofilm reactor(MBBR)applied to treat urban wastewater under different operational conditions[J]. Bioresource Technology,2012,121:119-126.
[4]袁兴程,李丹.生物绳填料净化河流的效果及微生物群落分析[J].环境工程,2016,34(4):48-53.
[5]吴智仁,彭娇,吴春笃,等.新型生物接触氧化载体及其实验分析[J].水处理技术,2016(1):52-55.
[6]国家环境保护总局《水和废水监测方法》编委会.水和废水监测分析方法[M].北京:中国环境科学出版社,2002.
[7] Ju F,Guo F,Ye L,et al. Metagenomic analysis on seasonal microbial variations of activated sludge from a full-scale wastewater treatment plant over 4 years[J]. Environmental Microbiology Reports,2014,6(1):80-89.
[8] Yuan X C,Qian X,Zhang B,et al. Performance and microbial community analysis of a novel bio-cord carrier during treatment of a polluted river[J]. Bioresource technology,2012,117:33-39.
[9] Zhang R B,Qian X,Li H M,et al. Selection of optimal river water quality improvement programs using QUAL2K:a case study of taihu lake basin,China[J]. Science of the Total Environment,2012,431:278-285.
[10] Roesch L F, Fulthorpe R R,Riva A,et al. Pyrosequencing enumerates and contrasts soil microbial diversity[J]. International Society for Microbial Ecology,2007,1(4):283-290.
[11] Mc Lellan S L,Huse S M,Mueller S R,et al. Diversity and population structure of sewage-derived microorganisms in wastewater treatment plant influent[J]. Environment Microbiological,2010,12(2):378-392.
[12] Du J,Ren H Q,Geng J J,et al. Occurrence and abundance of tetracycline,sulfonamide resistance genes,and class integron in five wastewater treatment plants[J]. Environmental science and pollution research international,2014,21(12):7276-7284.
[13] Limpiyakorn T, Sonthiphand P, Rongsayamanont C, et al.Abundance of amo A genes of ammonia-oxidizing archaea and bacteria in activated sludge of full-scale wastewater treatment plants[J]. Bioresource Technology,2011,102(4):3694-3701.
[14] Hu M,Wang X H, Wen X H, et al. Microbial community structures in different wastewater treatment plants as revealed by454-pyrosequencing analysis[J]. Bioresource Technology,2012,117:72-79.
[15] Lu H J,Chandran K,Stensel D. Microbial ecology of denitrification in biological wastewater treatment[J]. Water Research,2014,64:237-254.
[16] Ma J X,Wang Z W, Yang Y,et al. Correlating microbial community structure and composition with aeration intensity in submerged membrane bioreactors by 454 high-throughput pyrosequencing[J]. Water Research,2013,47(2):859-869.
[17] He G H,Yi F,Zhou S,et al. Microbial activity and community structure in two terrace-type wetlands constructed for the treatment of domestic wastewater[J]. Ecological Enginnering,2014,67:198-205.
[18] Ma Z,Wen X H,Zhao F,et al. Effect of temperature variation on membrane fouling and microbial community structure in membrane bioreactor[J].Bioresource Technology,2013,133:462-481.
[19] Zhang T,Shao M F,Ye L. 454 pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants[J].International Society for Microbial Ecology,2012,6(6):1137-1147.
[20] Liao R H,Shen K,Li A M,et al. High-nitrate wastewater treatment in an expanded granular sludge bed reactor and microbial diversity using 454 pyrosequencing analysis[J]. Bioresource Technology,2013,134:190-197.
[21]孙广垠,安恩方,刘勇,等.不同生物膜载体净化微污染水挂膜及处理效果研究[J].给水排水,2017,43(6):48-53.