氯虫苯甲酰胺拌种对玉米种子活力及幼苗生长的影响
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摘要
为研究氯虫苯甲酰胺拌种对玉米种子萌发、幼苗生长及生理生化指标的影响,采用平皿法和盆栽法研究氯虫苯甲酰胺不同剂量(0.5、1、2、3、4 g/kg)拌种处理对玉米种子萌发、幼苗生长量(株高、根长、根冠比等)以及生理生化指标(保护酶、根系活力、叶绿素含量等)的影响。结果表明:氯虫苯甲酰胺在0.5～3 g/kg剂量下拌种处理能明显提高玉米种子发芽势、发芽率、发芽指数和活力指数;增加幼苗期生长量;诱导幼苗叶片中CAT、POD、SOD、PAL、PPO活性升高;提高根系活力、叶绿素含量、还原糖含量及可溶性蛋白含量。氯虫苯甲酰胺在4 mg/kg剂量时对玉米植株的生长有一定的抑制作用,但各项指标仍高于空白对照。结果表明:合适剂量的氯虫苯甲酰胺拌种可以显著促进玉米种子萌发和幼苗生长。
To study the effects of maize seed dressing with chlorantraniliprole on seed germination, seedling growth and physiological and biochemical indexes, plate experiments in incubator and indoor pot experiments were conducted to study the seed germination, seedling growth(plant height, root length, root shoot ratio, etc.),physiological and biochemical indexes(protective enzyme, root activity and chlorophyll content, etc.) of maize by seed dressing with different dosage(0.5, 1, 2, 3, 4 g/kg) of chlorantraniliprole. The results showed that chlorantraniliprole at 0.5-3 g/kg significantly increased the germination potential, germination rate,germination index, vigor index of maize and increment in seedling stage, induced the activity of CAT, POD,SOD, PAL and PPO in maize seedling leaves, and increased root activity, chlorophyll content, sugar content and soluble protein content. Chlorantraniliprole inhibited the growth of maize after treated with 4 mg/kg, but the indicators were still higher than that of the control. In conclusion, chlorantraniliprole at suitable dosage could significantly promote seed germination and seedling growth of maize.
To study the effects of maize seed dressing with chlorantraniliprole on seed germination, seedling growth and physiological and biochemical indexes, plate experiments in incubator and indoor pot experiments were conducted to study the seed germination, seedling growth(plant height, root length, root shoot ratio, etc.),physiological and biochemical indexes(protective enzyme, root activity and chlorophyll content, etc.) of maize by seed dressing with different dosage(0.5, 1, 2, 3, 4 g/kg) of chlorantraniliprole. The results showed that chlorantraniliprole at 0.5-3 g/kg significantly increased the germination potential, germination rate,germination index, vigor index of maize and increment in seedling stage, induced the activity of CAT, POD,SOD, PAL and PPO in maize seedling leaves, and increased root activity, chlorophyll content, sugar content and soluble protein content. Chlorantraniliprole inhibited the growth of maize after treated with 4 mg/kg, but the indicators were still higher than that of the control. In conclusion, chlorantraniliprole at suitable dosage could significantly promote seed germination and seedling growth of maize.
引文
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[7]李刚,张风文,姚晨涛,等.干旱胁迫下S-诱抗素对玉米幼苗生长及生理作用的影响[J].植物生理学报,2017(9):1711-1716.
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[10]宋雪慧,姜兴印,邢则森,等.噻虫啉拌种对玉米幼苗生理生化指标的影响[J].中国农学通报,2018,34(4).
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[14]房锋,姜兴印,纪春涛,等.种衣剂对山农饲玉7号玉米幼苗生长和相关酶活性的影响[J].农药学学报,009,11(1):98-103.
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[17]张悦,王涛,刘修堂,等.基于叶绿素评价茚虫威和氯虫苯甲酰胺对烟草幼苗生态安全性[J]农药科学与管理,2013,34(1):49-51.
[18]季正文.氯虫苯甲酰胺对稻纵卷叶螟防效评价及其对水稻生长发育的影响[D].扬州:扬州大学,2010.
[19]赵阳,杨进,季正文,等.氯虫苯甲酰胺和氟虫腈对稻纵卷叶螟的防效及对水稻生长与生理指标的影响[J].江苏农业科学,2010(6):190-192.
[20]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:169-172.
[21]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:214-217.
[22]于鸣凤,田文琳.葡萄叶片中多酚氧化酶的研究[J].黑龙江八一农垦大学学报,1999,11(1):32-34.
[23]汤章城.现代植物生理学实验指南[M].北京:高等教育出版社,2000:213-214.
[24]明华,胡春胜,张玉铭,等.浸提法测定玉米叶绿素含量的改进[J].玉米科学,2007,15(4):93-95.
[25]邹琦.植物生理生化实验指导[M].北京:中国农业出版社,1995.
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[27]张晓龙,王世光.小麦种子活力与产量的关系[J].四川农业大学学报,1989(01):11-14.
[28]卜成成,姜兴印,芦勇,等.噻虫啉拌种对小麦种子及幼苗生长的生理效应[J].中国农学通报,2017,33(21):1-6.
[29] Huang G B, Chai Q, Feng F X, et al. Effects of different tillage systems on soil properties, root growth, grain yield, and water use efficiency of winter wheat(Triticum aestivum L.)in arid northwest China[J].Journal of Integrative Agriculture,2012,11(8):1286-1296.
[30] Abdel L A A, Tran L P. Impacts of priming with silicon on the growth and tolerance of maize plants to alkaline stress[J].Frontiers in Plant Science,2016,7(14078).
[31] Amrita K, Brian E E, The phenylalanine ammonia-lyase gene family in raspberry. Tructure, expression, and evolution[J].Plant Physiology,2001,127(1):230-239.
[32]刘喜存,刘红彦,倪云霞,等.不同化学诱抗剂对金银花叶片防御酶系的影响[J].植物保护,2009(2):75-77.
[33] Hart E H, Onime L A, Davies T E, et al. The effects of PPO activity on the proteome of ingested red clover and implications for improving the nutrition of grazing cattle[J].Journal of Proteomics,2016,141:67-76.
[34]王雅玲,孙丽娜,杨代斌,等.戊唑醇和苯醚甲环唑种子处理对玉米幼苗生长的影响比较[J].作物杂志,2009(04):60-63
[35]郭相平,郭枫,刘展鹏,等.水分胁迫及复水对玉米光合速率及可溶性糖的影响[J].玉米科学,2008,16(06):68-70.
[36]柳光富,张志东,李春,等.氯虫苯甲酰胺200SC对高粱螟虫的控制及对高粱生长和产量的影响[J].西南师范大学学报,2011,36(03):156-160.
[37]何发林,姜兴印,尚佃龙,等.氯虫苯甲酰胺种子处理对花生地上及地下害虫的防治效果[J].植物保护学报,2019,46(1):239-248.
[2]杨桂秋,童怡春,杨辉斌,等.新型杀虫剂氯虫苯甲酰胺研究概述[J].世界农药,2012,34(1):31-34.
[3] Zhang R M, Dong J F, Chen J H, et al. The Sublethal effects of chlorantraniliprole on Helicoverpa armigera(Lepidoptera:Noctuidae)[J].Journal of Integrative Agriculture,2013,12(3):457-466.
[4] Li Y, Mao M, Li Y, et al. Modulations of high-voltage activated Ca2+channels in the central neurones of Spodoptera exigua by chlorantraniliprole[J].Physiological Entomology,2011,36(3):230-234.
[5] Teixeira L A, Andaloro J T. Diamide insecticides:Global efforts to address insect resistance stewardship challenges[J].Pesticide Biochemistry and Physiology,2013,106(3):76-78.
[6]宫庆涛,张坤鹏,武海斌,等.6种杀虫剂对铜绿丽金龟防治效果评价[J].果树学报,2016(12):1542-1549.
[7]李刚,张风文,姚晨涛,等.干旱胁迫下S-诱抗素对玉米幼苗生长及生理作用的影响[J].植物生理学报,2017(9):1711-1716.
[8]邢则森,姜兴印,孙石昂,等.低温胁迫下S-诱抗素拌种对玉米生理指标的影响[J].中国农学通报,2018,34(16).
[9]袁传卫,姜兴印,季守民,等.吡唑醚菌酯拌种对玉米种子及幼苗的生理效应[J].农药,2014,53(12):881-884.
[10]宋雪慧,姜兴印,邢则森,等.噻虫啉拌种对玉米幼苗生理生化指标的影响[J].中国农学通报,2018,34(4).
[11]陈士林,高山松,鲍恩付,等.种衣剂对玉米种子活力及苗期几个生理指标的影响[J].中国农学通报,2004,20(4):160-160.
[12]段强,赵国玲,姜兴印,等.吡虫啉拌种对玉米种子活力及其幼苗生长的影响[J].玉米科学,2012,20(6):63-69.
[13]郑铁军.玉米种衣剂对种子萌发和生长的影响[J].玉米科学,1997(2):50-52.
[14]房锋,姜兴印,纪春涛,等.种衣剂对山农饲玉7号玉米幼苗生长和相关酶活性的影响[J].农药学学报,009,11(1):98-103.
[15] Francis M I, Redondo A, Burns J K, et al. Soil application of imidacloprid and related SAR-inducing compounds produces effective and persistent control of citrus canker[J].European Journal of Plant Pathology,2009,124(2):283-292.
[16] Ford K A, Wildermuth M C. Neonicotinoid insecticides induce salicylate-associated plant defense responses[J]. Proc Natl Acad Sci USA,2010,107(41):17527-17532.
[17]张悦,王涛,刘修堂,等.基于叶绿素评价茚虫威和氯虫苯甲酰胺对烟草幼苗生态安全性[J]农药科学与管理,2013,34(1):49-51.
[18]季正文.氯虫苯甲酰胺对稻纵卷叶螟防效评价及其对水稻生长发育的影响[D].扬州:扬州大学,2010.
[19]赵阳,杨进,季正文,等.氯虫苯甲酰胺和氟虫腈对稻纵卷叶螟的防效及对水稻生长与生理指标的影响[J].江苏农业科学,2010(6):190-192.
[20]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:169-172.
[21]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:214-217.
[22]于鸣凤,田文琳.葡萄叶片中多酚氧化酶的研究[J].黑龙江八一农垦大学学报,1999,11(1):32-34.
[23]汤章城.现代植物生理学实验指南[M].北京:高等教育出版社,2000:213-214.
[24]明华,胡春胜,张玉铭,等.浸提法测定玉米叶绿素含量的改进[J].玉米科学,2007,15(4):93-95.
[25]邹琦.植物生理生化实验指导[M].北京:中国农业出版社,1995.
[26]段强,姜兴印,鲍静,等.吡虫啉拌种对高产夏玉米幼苗生长及其保护酶活性的影响[J].应用生态学报,2011,22(09):2482-2486.
[27]张晓龙,王世光.小麦种子活力与产量的关系[J].四川农业大学学报,1989(01):11-14.
[28]卜成成,姜兴印,芦勇,等.噻虫啉拌种对小麦种子及幼苗生长的生理效应[J].中国农学通报,2017,33(21):1-6.
[29] Huang G B, Chai Q, Feng F X, et al. Effects of different tillage systems on soil properties, root growth, grain yield, and water use efficiency of winter wheat(Triticum aestivum L.)in arid northwest China[J].Journal of Integrative Agriculture,2012,11(8):1286-1296.
[30] Abdel L A A, Tran L P. Impacts of priming with silicon on the growth and tolerance of maize plants to alkaline stress[J].Frontiers in Plant Science,2016,7(14078).
[31] Amrita K, Brian E E, The phenylalanine ammonia-lyase gene family in raspberry. Tructure, expression, and evolution[J].Plant Physiology,2001,127(1):230-239.
[32]刘喜存,刘红彦,倪云霞,等.不同化学诱抗剂对金银花叶片防御酶系的影响[J].植物保护,2009(2):75-77.
[33] Hart E H, Onime L A, Davies T E, et al. The effects of PPO activity on the proteome of ingested red clover and implications for improving the nutrition of grazing cattle[J].Journal of Proteomics,2016,141:67-76.
[34]王雅玲,孙丽娜,杨代斌,等.戊唑醇和苯醚甲环唑种子处理对玉米幼苗生长的影响比较[J].作物杂志,2009(04):60-63
[35]郭相平,郭枫,刘展鹏,等.水分胁迫及复水对玉米光合速率及可溶性糖的影响[J].玉米科学,2008,16(06):68-70.
[36]柳光富,张志东,李春,等.氯虫苯甲酰胺200SC对高粱螟虫的控制及对高粱生长和产量的影响[J].西南师范大学学报,2011,36(03):156-160.
[37]何发林,姜兴印,尚佃龙,等.氯虫苯甲酰胺种子处理对花生地上及地下害虫的防治效果[J].植物保护学报,2019,46(1):239-248.