HMGN2、CyclinD1在宫颈癌中的表达及临床意义
摘要
目的探讨高迁移率核小体蛋白N2(HMGN2)、细胞周期蛋白D1(CyclinD1)在宫颈癌中的表达及意义。方法选取四川大学华西第二医院病理科2014年7月～2015年9月临床病理资料完整的石蜡标本。采用免疫组化染色,联合检测HMGN2、CyclinD1在正常宫颈(20例)、宫颈上皮内瘤变(CIN)(30例)、宫颈癌(50例)中的表达,分析两者与宫颈癌临床病理特征的关系。结果 HMGN2在正常宫颈组、CIN组、宫颈癌组的阳性表达率分别为90.00%、70.00%、44.00%,差异有统计学意义(P<0.05);CyclinD1在正常宫颈组、CIN组、宫颈癌组的阳性表达率分别为30.00%、56.67%、64.00%,差异有统计学意义(P<0.05)。HMGN2阳性表达与宫颈癌的分化程度有关,但HMGN2、CyclinD1的表达与宫颈癌的临床分期、病理类型、宫颈间质浸润深度、脉管浸润及淋巴结转移无关;宫颈癌中HMGN2与CyclinD1的表达呈低度相关(Pearson列联系数为0.26)。结论 HMGN2低表达及CyclinD1过表达可能与宫颈癌发生发展有关。
引文
[1] FURUSAWA T,CHERUKURI S.Developmental function of HMGN proteins.Biochim Biophys Acta,2010,1799(1):69-73.
[2] 于承仟,沈帅,王雪,等.高迁移率族蛋白N2在肿瘤中的研究进展.现代肿瘤医学,2016,24(19):3151-3153.
[3] PORKKA K,LAAKKONEN P,HOFFMAN JA,et al.A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo.PNAS,2002,99(11):7444-7449.
[4] ISOAI A,GIGA-HAMA Y,SHINKAI K,et al.Tumor invasion-inhibiting factor 2:primary structure and inhibitory effect on invasion in vitro and pulmonary metastasis of tumor cells.Cancer Res,1992,52(6):1422-1426.
[5] LIANG GJ,XUE J,YANG CQ,et al.Nucleosome-binding protein HMGN2 exhibits antitumor activity in human SaO2 and U2-OS osteosarcoma cell lines.Oncol Rep,2015,33(3):1300-1306.
[6] XIONG WB,HUANG N,FENG Y,et al.Creation and anti-cancer potency in HeLa cells of a novel chimeric toxin,HMGNCIDIN,composed of HMGN2 α-helical domain and PE38 KDEL domain Ⅲ.Chin Med J (Engl),2008,121(1):82-85.
[7] 罗斌,张倩雯,许钰,等.高迁移率族蛋白N2抗裸鼠宫颈癌移植瘤的作用研究.四川大学学报(医学版),2016,47(5):718-721.
[8] GAUTSCHI O,HUGLI B,ZIEGLER A,et al.Cyclin D1 (CCND1) A870G gene polymorphism modulates smoking-induced lung cancer risk and response to platinum-based chemotherapy in non-small cell lung cancer (NSCLC) patients.Lung Cancer,2006,51(3):303-311.
[9] PARKM T,LEES J.Cell cycle and cancer.J Biochem Mol Biol,2003,36(1):60-65.
[2] 于承仟,沈帅,王雪,等.高迁移率族蛋白N2在肿瘤中的研究进展.现代肿瘤医学,2016,24(19):3151-3153.
[3] PORKKA K,LAAKKONEN P,HOFFMAN JA,et al.A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo.PNAS,2002,99(11):7444-7449.
[4] ISOAI A,GIGA-HAMA Y,SHINKAI K,et al.Tumor invasion-inhibiting factor 2:primary structure and inhibitory effect on invasion in vitro and pulmonary metastasis of tumor cells.Cancer Res,1992,52(6):1422-1426.
[5] LIANG GJ,XUE J,YANG CQ,et al.Nucleosome-binding protein HMGN2 exhibits antitumor activity in human SaO2 and U2-OS osteosarcoma cell lines.Oncol Rep,2015,33(3):1300-1306.
[6] XIONG WB,HUANG N,FENG Y,et al.Creation and anti-cancer potency in HeLa cells of a novel chimeric toxin,HMGNCIDIN,composed of HMGN2 α-helical domain and PE38 KDEL domain Ⅲ.Chin Med J (Engl),2008,121(1):82-85.
[7] 罗斌,张倩雯,许钰,等.高迁移率族蛋白N2抗裸鼠宫颈癌移植瘤的作用研究.四川大学学报(医学版),2016,47(5):718-721.
[8] GAUTSCHI O,HUGLI B,ZIEGLER A,et al.Cyclin D1 (CCND1) A870G gene polymorphism modulates smoking-induced lung cancer risk and response to platinum-based chemotherapy in non-small cell lung cancer (NSCLC) patients.Lung Cancer,2006,51(3):303-311.
[9] PARKM T,LEES J.Cell cycle and cancer.J Biochem Mol Biol,2003,36(1):60-65.