饲喂不同处理的蚯蚓对小白鼠生长及免疫性能的影响
|
摘要
为了研究不同处理的蚯蚓对小白鼠生长及免疫性能的影响,试验选取21日龄的昆明小鼠75只,随机分为5个组,每天分别灌喂0.2 mL未经处理、烘干处理、微酸性电解水处理、高锰酸钾处理的蚯蚓匀浆,灌喂等量生理盐水作为空白对照组。4周后检测小鼠的生长性能指标和免疫机能指标。结果表明:各处理间小鼠体重、日采食量、日增重和料重比均无明显差异(P>0.05),即每天灌喂0.2 mL蚯蚓匀浆对小鼠生长性能没有显著影响;与对照组相比,灌喂未经处理和高锰酸钾处理的蚯蚓匀浆均显著(P<0.05)提高了小鼠胸腺指数、脾脏指数、超氧化物歧化酶(SOD)水平和过氧化氢酶(CAT)水平,饲喂经微酸性电解水处理后的蚯蚓匀浆,提高了小鼠除胸腺指数外的其他免疫指标(P<0.05)。说明每天饲喂蚯蚓匀浆对小鼠的生长性能没有显著影响,但是除烘干处理外,其他处理的蚯蚓匀浆均能使小鼠的免疫性能显著提升。
This experiment was conducted to study the effect of different treated earthworms on growth and immune performance of mice. 75 21-day-old Kunming mice were randomly divided into five groups equally,which were fed 0.2 mL untreated, dry treated, micro-acidic electrolytic water treated and potassium permanganate treated earthworm homogenate every day separately.The blank control group was fed the same amount of saline. The growth performance and immune function of mice were measured after four weeks. There was no significant difference in weight, daily intake, daily gain and weight ratio among each treatment group(P>0.05), that is, daily feeding of 0.2 mL the earthworm homogenate had no significant effect on the growth performance of mice. Compared with the blank control group, feeding untreated and potassium permanganate treated earthworm homogenate significantly( P<0.05) increased thymus index, spleen index, superoxide dismutase(SOD) levels and catalase(CAT) level of mice, feeding micro-acidic electrolytic water treated earthworms homogenate significantly(P<0.05)improved the immune indexes except thymus index of mice. The daily feeding of earthworm homogenate had no significant effect on the growth performance of mice, however, with the exception of drying treatment,other earthworm treatments could significantly improve the immune performance of mice.
This experiment was conducted to study the effect of different treated earthworms on growth and immune performance of mice. 75 21-day-old Kunming mice were randomly divided into five groups equally,which were fed 0.2 mL untreated, dry treated, micro-acidic electrolytic water treated and potassium permanganate treated earthworm homogenate every day separately.The blank control group was fed the same amount of saline. The growth performance and immune function of mice were measured after four weeks. There was no significant difference in weight, daily intake, daily gain and weight ratio among each treatment group(P>0.05), that is, daily feeding of 0.2 mL the earthworm homogenate had no significant effect on the growth performance of mice. Compared with the blank control group, feeding untreated and potassium permanganate treated earthworm homogenate significantly( P<0.05) increased thymus index, spleen index, superoxide dismutase(SOD) levels and catalase(CAT) level of mice, feeding micro-acidic electrolytic water treated earthworms homogenate significantly(P<0.05)improved the immune indexes except thymus index of mice. The daily feeding of earthworm homogenate had no significant effect on the growth performance of mice, however, with the exception of drying treatment,other earthworm treatments could significantly improve the immune performance of mice.
引文
[1]涂清波,林颖,苏鹏亮,等.蚯蚓的化学成分与应用价值研究进展[J].安徽农业科学,2017,45(34):109-111.
[2]范涛,刘毅,明伟,等.蚯蚓粉替代鱼粉对大鳞副泥鳅生长、肌肉成分、血清生化指标及免疫性能的影响[J].中国水产科学,2016,23(6):1320-1331.
[3]白燕,王维新,迟进坤,等.蚯蚓干粉对幼刺参生长、消化和免疫力的影响[J].中国农学通报,2012,28(29):120-124.
[4]李荣.蚯蚓活性肽对小鼠免疫机能的影响[D].长沙:湖南农业大学,2008.
[5]徐炜虹,杨齐衡,路英华,等.蚯蚓过氧化氢酶的纯化及性质[J].华东师范大学学报(自然科学版),1996,42(4):95-101.
[6]丁佩惠,唐琪,陈莉丽,等.中药地龙提取液的促成骨作用及对试验性牙槽骨吸收疗效的研究[J].浙江大学学报(理学版),2008,53(6):684-689.
[7]刘廷强,严泽民,周华峰,等.蚯蚓小分子多肽提取物的制备及抗氧化活性[J].江苏农业科学,2016,44(7):463-466.
[8]廖怡,荣永海,荣龙.从蚯蚓中联合提取抗氧化酶SOD、CAT的方法研究[J].天然产物研究与开发,2012,24(11):1538-1544.
[9]李娟,徐玉玲,王永香,等.地龙体外抗凝血部位的制备工艺研究[J].中国中药杂志,2011,36(7):850-853.
[10]涂清波,苏鹏亮,林颖,等.蚓激酶的提取工艺改进研究[J].天津中医药,2018,35(1):65-67.
[11]蒋桂林.由蚯蚓作为媒介引起的鸡寄生虫病的调查及控制[J].陕西农业科学,2012,58(4):274-275.
[12]张显忠,张欣然,武晓雯,等.蚯蚓体内外分泌液的抗菌活性研究[J].安徽农业科学,2018,46(13):95-97.
[13]Wang C,Sun Z J,Zheng D M.Function of mucilaginous secretions in the antibacterial immunity system of Eisenia fetida[J].Pedobiologia,2011,54(1):7-62
[14]王燕丽,李君荣,吴春金,等.复合发酵蚯蚓蛋白饲料对肉猪生长性能的效果试验[J].饲料与畜牧,2013,27(8):11-13.
[15]张泳桢,吴华东,吴武平,等.蚯蚓粉替代日粮中部分豆粕对生长猪生产性能和免疫指标的影响[J].饲料工业,2016,37(3):22-25.
[16]刁治民,王生财,邓君,等.蚯蚓的经济价值及开发应用前景[J].青海草业,2005,14(3):7-12,15.
[17]朱宇旌,李维,张勇,等.蚯蚓粉对肉鸡生长性能、营养物质代谢及免疫功能的影响[J].沈阳农业大学学报,2010,41(6):695-700.
[18]丁晓,杨在宾,任小杰.饲用抗生素替代品对肉鸡生产性能、抗氧化性能、免疫性能和肠道菌群的影响[J].中国家禽,2018,40(10):21-26.
[19]张笑天,郑晓瑛.氧化自由基清除剂超氧化物歧化酶与疾病[J].中国公共卫生,2014,30(10):1349-1352.
[20]Prakash M,Gunasekaran G,Elumalai K.Effect of earthworm powder on antioxidant enzymes in alcohol induced hepatotoxic rats[J].European Review for Medical and Pharmacological Sciences,2008,12(4):237-243.
[21]汪倬,周骏江,周念波,等.蚯蚓作为新型动物性蛋白饲料的可行性研究[J].安徽农业科学,2008,48(13):5467-5468.
[2]范涛,刘毅,明伟,等.蚯蚓粉替代鱼粉对大鳞副泥鳅生长、肌肉成分、血清生化指标及免疫性能的影响[J].中国水产科学,2016,23(6):1320-1331.
[3]白燕,王维新,迟进坤,等.蚯蚓干粉对幼刺参生长、消化和免疫力的影响[J].中国农学通报,2012,28(29):120-124.
[4]李荣.蚯蚓活性肽对小鼠免疫机能的影响[D].长沙:湖南农业大学,2008.
[5]徐炜虹,杨齐衡,路英华,等.蚯蚓过氧化氢酶的纯化及性质[J].华东师范大学学报(自然科学版),1996,42(4):95-101.
[6]丁佩惠,唐琪,陈莉丽,等.中药地龙提取液的促成骨作用及对试验性牙槽骨吸收疗效的研究[J].浙江大学学报(理学版),2008,53(6):684-689.
[7]刘廷强,严泽民,周华峰,等.蚯蚓小分子多肽提取物的制备及抗氧化活性[J].江苏农业科学,2016,44(7):463-466.
[8]廖怡,荣永海,荣龙.从蚯蚓中联合提取抗氧化酶SOD、CAT的方法研究[J].天然产物研究与开发,2012,24(11):1538-1544.
[9]李娟,徐玉玲,王永香,等.地龙体外抗凝血部位的制备工艺研究[J].中国中药杂志,2011,36(7):850-853.
[10]涂清波,苏鹏亮,林颖,等.蚓激酶的提取工艺改进研究[J].天津中医药,2018,35(1):65-67.
[11]蒋桂林.由蚯蚓作为媒介引起的鸡寄生虫病的调查及控制[J].陕西农业科学,2012,58(4):274-275.
[12]张显忠,张欣然,武晓雯,等.蚯蚓体内外分泌液的抗菌活性研究[J].安徽农业科学,2018,46(13):95-97.
[13]Wang C,Sun Z J,Zheng D M.Function of mucilaginous secretions in the antibacterial immunity system of Eisenia fetida[J].Pedobiologia,2011,54(1):7-62
[14]王燕丽,李君荣,吴春金,等.复合发酵蚯蚓蛋白饲料对肉猪生长性能的效果试验[J].饲料与畜牧,2013,27(8):11-13.
[15]张泳桢,吴华东,吴武平,等.蚯蚓粉替代日粮中部分豆粕对生长猪生产性能和免疫指标的影响[J].饲料工业,2016,37(3):22-25.
[16]刁治民,王生财,邓君,等.蚯蚓的经济价值及开发应用前景[J].青海草业,2005,14(3):7-12,15.
[17]朱宇旌,李维,张勇,等.蚯蚓粉对肉鸡生长性能、营养物质代谢及免疫功能的影响[J].沈阳农业大学学报,2010,41(6):695-700.
[18]丁晓,杨在宾,任小杰.饲用抗生素替代品对肉鸡生产性能、抗氧化性能、免疫性能和肠道菌群的影响[J].中国家禽,2018,40(10):21-26.
[19]张笑天,郑晓瑛.氧化自由基清除剂超氧化物歧化酶与疾病[J].中国公共卫生,2014,30(10):1349-1352.
[20]Prakash M,Gunasekaran G,Elumalai K.Effect of earthworm powder on antioxidant enzymes in alcohol induced hepatotoxic rats[J].European Review for Medical and Pharmacological Sciences,2008,12(4):237-243.
[21]汪倬,周骏江,周念波,等.蚯蚓作为新型动物性蛋白饲料的可行性研究[J].安徽农业科学,2008,48(13):5467-5468.