儿茶酚、香豆素对豌豆蚜生长发育及繁殖的影响
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摘要
儿茶酚、香豆素是植物的次生代谢物质对桃蚜等有拒食功能,但是对豌豆蚜的作用尚未见报道。为了明确儿茶酚、香豆素对红、绿色型豌豆蚜(Acyrthosiphon pisum)生理的影响。本研究采用人工饲料喂养技术测定了豌豆蚜的生理指标。结果表明:随着两种物质浓度的增加,两种色型蚜虫的存活率、繁殖率都在显著下降,但在相对日均体重增长率之间差异不明显。香豆素对红色型的繁殖率影响比对绿色型的更加明显。红色型在60mg·L~(-1)处理时已经为零,而绿色型在100mg·L~(-1)时依然有繁殖。红色型的存活率、产蚜率、平均产蚜量比绿色型的更容易受到儿茶酚的影响,在儿茶酚浓度最高时红色型的存活率在时间顺序上分别是73.30±1.50%,20.00±0.00%,0.00±0.00%,绿色型的为66.60±0.80%,26.7±2.00%,3.30±0.10%。而在儿茶酚浓度最低时红色型的平均产蚜量、产蚜率为3.70%±0.80%,16.70±0.60%,绿色型为9.00±0.60%,30.0±0.70%。这说明绿色型比红色型对香豆素、儿茶酚的耐受性更好。
Catechol and coumarin are plant secondary metabolites that have antifeedant functions against peach aphid,but the effects on pea mites have not been reported.In order to clarify the effects of in vitro catechol and coumarin on growth and reproduction of red and green peas aphids(Acyrthosiphon pisum).In this study,artificial feed feeding techniques were used to determine the survival rate,average progeny produced,production rate and relative average daily growth rate of pea aphids.The results showed that the survival rate and reproduction rate of two color-type pea aphids decreased significantly with the increase of catechol and coumarin concentrations.However,there is no significant difference between the relative daily average body weight gain rates.The effect of coumarin on the reproduction rate of red type is more obvious than that of green type.The red type was already zero when treated at 60 mg·L~(-1) and the green type was still propagated at 100 mg·L~(-1).The survival rate,calving rate,and average calving yield of red type are more susceptible to catechol than green type.When the catechol concentration is the highest,the reproduction rate of red type on the 6 d,12 d,and 20 dwas 73.3±1.5,20.0±0.0,and 0.0±0.0,and the green type was 66.6±0.8,26.7±2.0,and 3.3±0.1.When the concentration of catechol is the lowestn,the average yield of calyx and calving rate were 3.7±0.8,16.7±0.6,and that of green type were 9.0±0.6,30.0±0.7.The results indicates that the green type is more resistant to coumarin and catechol than the red type.
Catechol and coumarin are plant secondary metabolites that have antifeedant functions against peach aphid,but the effects on pea mites have not been reported.In order to clarify the effects of in vitro catechol and coumarin on growth and reproduction of red and green peas aphids(Acyrthosiphon pisum).In this study,artificial feed feeding techniques were used to determine the survival rate,average progeny produced,production rate and relative average daily growth rate of pea aphids.The results showed that the survival rate and reproduction rate of two color-type pea aphids decreased significantly with the increase of catechol and coumarin concentrations.However,there is no significant difference between the relative daily average body weight gain rates.The effect of coumarin on the reproduction rate of red type is more obvious than that of green type.The red type was already zero when treated at 60 mg·L~(-1) and the green type was still propagated at 100 mg·L~(-1).The survival rate,calving rate,and average calving yield of red type are more susceptible to catechol than green type.When the catechol concentration is the highest,the reproduction rate of red type on the 6 d,12 d,and 20 dwas 73.3±1.5,20.0±0.0,and 0.0±0.0,and the green type was 66.6±0.8,26.7±2.0,and 3.3±0.1.When the concentration of catechol is the lowestn,the average yield of calyx and calving rate were 3.7±0.8,16.7±0.6,and that of green type were 9.0±0.6,30.0±0.7.The results indicates that the green type is more resistant to coumarin and catechol than the red type.
引文
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[16]邵娅,王森山,叶超.单宁酸对红、绿色型豌豆蚜生长发育及繁殖的影响[J].草地学报,2017,25(4):865-870
[17]卢继荣.马桑籽中杀虫活性成分的初步研究[D].杨凌:西北农林科技大学,2004,2-40
[18]陶茸,尹国丽,师尚礼.香豆素和咖啡酸对紫花苜蓿根尖和根缘细胞发育的影响[J].草地学报,2018,26(04):869-876
[19]陈巨莲,倪汉祥等.几种主要次生物质对麦长管蚜几种酶活力的影响[J].昆虫学报,2003,46(2):144-149
[20]武德功.豌豆蚜地理种群遗传多样性及其种群调控机制研究[D].兰州:甘肃农业大学,2011:7-8
[21]武德功,贺春贵,等.不同首蓓品种对豌豆蚜的生化抗性机制[J].草地学报,2011(3):497-501
[2]特木尔布和,乌日图,金小龙,等.蚜虫对苜蓿危害的初步研究[J].内蒙古草业,2005,17(4):56-59
[3]杜军利.两种色型豌豆蚜种群数量变化机制研究[D].兰州:甘肃农业大学,2013,1-2
[4]程璐.苜蓿生理生化抗蚜机制初步研究[D].兰州:甘肃农业大学,2009,3-6
[5]张文辉,刘光杰.植物抗虫性次生物质的研究概况[J].植物学通报,2003(05):522-530
[6]彭少麟,南蓬.高等植物中的萜类化合物及其在生态系统中的作用[J].生态学杂志,02,21(3):33-38
[7]徐正浩,崔绍荣,何勇,等.植物次生代谢物质和害虫防治[J].植物保护,004,30(4):8-11
[8]张韶瑜,孟林,高文远,等.香豆素类化合物生物学活性研究进展[J].中国中药杂志,2005,30(6):41
[9]王超,严清平.蛇床子素防治板蓝根霜霉病、黄瓜瓜绢螟的应用效果[J].江苏农业学报,2003,(增刊):144-146
[10]王志琦,沈寿国.蛇床子素对植物病原菌抑制机制的初步研究[J].农药学学报,2004,6(4):28-32
[11]李延.儿茶酚胁迫下铜绿微囊藻的生理生化响应[J].环境工程学报,2016,10(6):2781-2785
[12]陈巨莲,倪汉祥,孙京瑞.主要次生物质对麦蚜的抗性阈值及交互作用[J].植物保护学报,2002,29(1):7-12
[13]Leszczynski B,Wright L C,Niraz S.The influence of phenolic compounds on the preference of winter wheat cultivars cereal aphid[J].Insect science and Its application,1985,6(2):157-158
[14]寇江涛,师尚礼.紫花苜蓿对牛角花齿蓟马为害的生理响应研究[J].草地学报,2015,23(01):143-150
[15]叶超.豌豆蚜人工饲料及饲养技术研究[D].兰州:甘肃农业大学硕士论文,2016:21-23
[16]邵娅,王森山,叶超.单宁酸对红、绿色型豌豆蚜生长发育及繁殖的影响[J].草地学报,2017,25(4):865-870
[17]卢继荣.马桑籽中杀虫活性成分的初步研究[D].杨凌:西北农林科技大学,2004,2-40
[18]陶茸,尹国丽,师尚礼.香豆素和咖啡酸对紫花苜蓿根尖和根缘细胞发育的影响[J].草地学报,2018,26(04):869-876
[19]陈巨莲,倪汉祥等.几种主要次生物质对麦长管蚜几种酶活力的影响[J].昆虫学报,2003,46(2):144-149
[20]武德功.豌豆蚜地理种群遗传多样性及其种群调控机制研究[D].兰州:甘肃农业大学,2011:7-8
[21]武德功,贺春贵,等.不同首蓓品种对豌豆蚜的生化抗性机制[J].草地学报,2011(3):497-501