N末端改造提高GH11家族木聚糖酶热稳定性的研究进展
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摘要
木聚糖酶具有重要的工业应用价值,在食品、饲料、造纸等领域有着良好的发展前景。不同应用领域对木聚糖酶的酶学性质具有不同要求,耐热性是木聚糖酶在实际生产过程中最有应用价值的性质之一;因此,许多研究者致力于木聚糖酶耐热性机制的研究。研究发现GH11家族木聚糖酶的热稳定性与其N末端区域密切相关,可以通过N末端改造的策略有效提升GH11家族木聚糖酶的热稳定性。本文主要综述了影响GH11家族木聚糖酶热稳定性的因素、N末端改造提升木聚糖酶热稳定性的技术和方法,并对耐热木聚糖酶的应用前景进行展望,以期为GH11家族木聚糖酶的耐热性研究提供一定的参考。
Xylanases have great potential industrial applications and are promising in the food, feed and paper-making industries. The application of xlanases in different fields is due to their versatile enzymatic properties, especially the outstanding thermostability. Therefore, the heat resistance mechanism of xylanases attracts much research interest.The thermostability of GH11 xylanases has been closely related to their N-terminal region, and many researchers have successfully constructed thermophilic xylanases by N-terminal modi?cation. This paper reviews the factors in?uencing the thermostability of GH11 xylanases and the different techniques and methods used for N-terminal modi?cation of xylanase for improving heat resistance, and prospects for possible future applications are discussed. This review is expected to provide valuable information for improving the thermostability of GH11 family xylanases.
Xylanases have great potential industrial applications and are promising in the food, feed and paper-making industries. The application of xlanases in different fields is due to their versatile enzymatic properties, especially the outstanding thermostability. Therefore, the heat resistance mechanism of xylanases attracts much research interest.The thermostability of GH11 xylanases has been closely related to their N-terminal region, and many researchers have successfully constructed thermophilic xylanases by N-terminal modi?cation. This paper reviews the factors in?uencing the thermostability of GH11 xylanases and the different techniques and methods used for N-terminal modi?cation of xylanase for improving heat resistance, and prospects for possible future applications are discussed. This review is expected to provide valuable information for improving the thermostability of GH11 family xylanases.
引文
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