生物炭固定菌强化人工湿地对低温污水中氮素去除的模拟研究
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摘要
以从冬季南四湖湿地底泥中分离筛选出的黄假单胞菌(Pseudomonas flava WD-3)为微生物载体,采用水稻秸秆炭化制备的优质生物炭为吸附剂,将生物炭固定化Pseudomonas flava WD-3投加到垂直流人工湿地污水处理系统中,探讨不同菌剂接种量对低温污水中氮素的去除情况;并采用简化的Monod动力学模型对人工湿地污水中污染物的去除过程进行模拟。结果表明,在水力停留时间5 d时,生物炭固定化Pseudomonas flava WD-3对人工湿地低温污水中氮素的去除效率有显著提高,且对人工湿地污水中TN、NH_4~+-N、NO_3~--N和NO_2~--N的降解符合Monod动力学模型。
A kind of psychrophile strain named pseudomonas flava WD-3 was isolated from the sediments of Nansihu Lake in winter,and it was used as the microbial carrier. The carbonized rice straw was prepared to be biocarbon as adsorbent,and the biochar-fixed Pseudomonas flava WD-3 bacteria was added into the vertical flow constructed wetland sewage treatment system to discuss the removal efficiency of nitrogen in wastewater of low temperature and to simulate the kinetics of degradation of pollutants in constructed wetland by a simplified Monod Model. The results demonstrate that the removal efficiency of nitrogen in waste water in constructed wetland was increased significantly,when hydraulic retention time was 5 d. The degradation kinetics of TN,NH_4~+-N,NO_2~--N,NO_3~--N were accorded with the Monod Model by biochar-fixed Pseudomonas flava WD-3.
A kind of psychrophile strain named pseudomonas flava WD-3 was isolated from the sediments of Nansihu Lake in winter,and it was used as the microbial carrier. The carbonized rice straw was prepared to be biocarbon as adsorbent,and the biochar-fixed Pseudomonas flava WD-3 bacteria was added into the vertical flow constructed wetland sewage treatment system to discuss the removal efficiency of nitrogen in wastewater of low temperature and to simulate the kinetics of degradation of pollutants in constructed wetland by a simplified Monod Model. The results demonstrate that the removal efficiency of nitrogen in waste water in constructed wetland was increased significantly,when hydraulic retention time was 5 d. The degradation kinetics of TN,NH_4~+-N,NO_2~--N,NO_3~--N were accorded with the Monod Model by biochar-fixed Pseudomonas flava WD-3.
引文
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[3]LEHMANN J,RILLIG M C,THIES J,et al.Biochar Effects on Soil Biota:A Review[J].Soil Biology and Biochemistry,2011,43(9):1812-1836.
[4]付融冰.强化人工湿地对富营养化水体的修复及作用机理研究[D].上海:同济大学.2007.[FU Rong-bing.Remediation of Eutrophic Water and Mechanism Using Enhanced Constructed Wetlands[D].Shanghai:Tongji University,2007.]
[5]唐美珍,李婷婷,王艳娜,等.人工湿地中一株高效低温菌的分离鉴定与去除特性研究[J].环境科学学报,2013,33(3):708-714.[TANG Mei-zhen,LI Ting-ting,WANG Yan-na,et al.Identification and Characterization of an Efficient Psychrotrophic Bacterial Strain Isolated From Artificial Wetlands[J].Acta Scientiae Circumstantiae,2013,33(3):708-714.]
[6]唐美珍,汪文飞,李如如,等.生物炭对Pseudomonas flava WD-3的固定化及其强化人工湿地污水处理研究[J].环境科学学报,2017,37(9):3441-3448.[TANG Mei-zhen,WANG Wen-fei,LI Ru-ru,et al.Immobilized Pseudomonas flava WD-3 by Biochar for the Sewage Purification in the Artificial Wetland[J].Acta Scientiae Circumstantiae,2017,37(9):3441-3448.]
[7]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002:254-284.
[8]唐美珍,姚淑敏,张凤凤,等.Pseudomonas flava WD-3对人工湿地污染物的去除及Monod模型模拟[J].环境科学学报,2014,34(12):3050-3056.[TANG Mei-zhen,YAO Shu-min,ZHANGFeng-feng,et al.Pollutants Removal by Pseudomonas flava WD-3and Monod Model Simulation in Constructed Wetland[J].Acta Scientiae Circumstantiae,2014,34(12):3050-3056.]
[9]SAEED T,SUN G Z.Kinetic Modelling of Nitrogen and Organics Removal in Vertical and Horizontal Flow Wetlands[J].Water Research,2011,45(10):3137-3152.
[10]郑朔方,杨苏文,金相灿.铜绿微囊藻生长的营养动力学[J].环境科学,2005,26(2):152-156.[ZHENG Shuo-fang,YANG Suwen,JIN Xiang-can.Dynamic Studies on the Effect of Nutrients on the Growth of Microcystis aeruginosa[J].Environmental Science,2005,26(2):152-156.]
[11]WIESMANN U.Biological Nitrogen Removal From Wastewater[J].Advances in Biochemical Engineering/Biotechnology,1994,51:113-154.
[12]祖波,张代钧,阎青,等.微量NO2对厌氧氨氧化甲烷化反硝化耦合影响的动力学分析[J].环境工程学报,2008,2(9):1223-1227.[ZU Bo,ZHANG Dai-jun,YAN Qing,et al.The Kinetics A-nalysis on Effect of Trace NO2on Integrating Anaerobic Ammonium Oxidation,Methanogenesis and Denitrification[J].Chinese Journal of Environmental Engineering,2008,2(9):1223-1227.]