闽南地区薏米叶斑病菌对吡唑醚菌酯的敏感性及吡唑醚菌酯的盆栽防治效果
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摘要
采用菌丝生长速率法测定了闽南地区4个薏米产区72株薏米叶斑病菌对吡唑醚菌酯的敏感性,并通过室内盆栽试验测试了吡唑醚菌酯对薏米叶斑病的防治效果。结果表明:吡唑醚菌酯对供试菌株的EC50值范围为1.11～5.06μg/mL,平均值为(3.44±0.98)μg/mL,且其EC50值频率分布为连续单峰曲线,符合正态分布,因此可将该EC50平均值作为闽南地区薏米叶斑病菌对吡唑醚菌酯的敏感基线。盆栽防效结果表明,喷施有效剂量为250μg/mL的25%吡唑醚菌酯乳油对薏米叶斑病的防治效果(78.2%～79.7%)较好,与喷施有效剂量为500μg/mL的50%异菌脲悬浮剂的防治效果相当,显著高于喷施有效剂量为800μg/mL的80%代森锰锌可湿性粉剂的防治效果。本研究结果为薏米叶斑病化学防治有效药剂的选择与合理使用提供了依据。
Seventy-two Curvularia coicis isolates from 4 regions in South Fujian Province were tested for their sensitivity to pyraclostrobin using mycelial growth method. And the efficacy of pyraclostrobin for the control of Coix leaf blight(CLB) was evaluated under greenhouse conditions. The results indicated that the tested isolates were sensitive to pyraclostrobin. And the EC_(50) values of these isolates to pyraclostrobin ranged from 1.11 μg/mL to 5.06 μg/mL, with the mean value of(3.44 ± 0.98) μg/mL.The frequency curve of pyraclostrobin was continuous and unimodal, and following the normal distribution. Hence, the mean EC_(50) value of(3.44 ± 0.98) μg/mL can be recognized as the baseline sensitivity of C. coicis to pyraclostrobin. Results of pot experiments showed that the spray of 25%pyraclostrobin EC at 250 μg a.i./mL exhibited high efficacy for the control of CLB, with the control efficacy ranging from 78.2% to 79.7%. This efficacy was as good as that resulted from the spray of 50% iprodione SC at 500 μg a.i./mL, and significantly higher than the efficacy of reference fungicide(80%mancozeb WP at 800 μg a.i./mL). This study will guide the reasonable selection of fungicides for effective management of CLB in the future.
Seventy-two Curvularia coicis isolates from 4 regions in South Fujian Province were tested for their sensitivity to pyraclostrobin using mycelial growth method. And the efficacy of pyraclostrobin for the control of Coix leaf blight(CLB) was evaluated under greenhouse conditions. The results indicated that the tested isolates were sensitive to pyraclostrobin. And the EC_(50) values of these isolates to pyraclostrobin ranged from 1.11 μg/mL to 5.06 μg/mL, with the mean value of(3.44 ± 0.98) μg/mL.The frequency curve of pyraclostrobin was continuous and unimodal, and following the normal distribution. Hence, the mean EC_(50) value of(3.44 ± 0.98) μg/mL can be recognized as the baseline sensitivity of C. coicis to pyraclostrobin. Results of pot experiments showed that the spray of 25%pyraclostrobin EC at 250 μg a.i./mL exhibited high efficacy for the control of CLB, with the control efficacy ranging from 78.2% to 79.7%. This efficacy was as good as that resulted from the spray of 50% iprodione SC at 500 μg a.i./mL, and significantly higher than the efficacy of reference fungicide(80%mancozeb WP at 800 μg a.i./mL). This study will guide the reasonable selection of fungicides for effective management of CLB in the future.
引文
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[11]FERNáNDEZ-ORTU?O D,CHEN F,SCHNABEL G.Resistance to pyraclostrobin and boscalid in Botrytis cinerea isolates from strawberry fields in the Carolinas[J].Plant Dis,2012,96(8):1198-1203.
[12]WISE K A,BRADLEY C A,PASCHE J S,et al.Resistance to QoIfungicides in Ascochyta rabiei from chickpea in the Northern Great Plains[J].Plant Dis,2009,93(5):528-536.
[13]AVENOT H,MORGAN D P,MICHAILIDES T J.Resistance to pyraclostrobin,boscalid and multiple resistance to Pristine?(pyraclostrobin+boscalid)fungicide in Alternaria alternate causing alternaria late blight of pistachios in California[J].Plant Pathol,2008,57(1):135-140.
[14]FERNáNDEZ-ORTU?O D,PéREZ-GARCíA A,LóPEZ-RUIZ F,et al.Occurrence and distribution of resistance to QoI fungicides in populations of Podosphaera fusca in south central Spain[J].Eur JPlant Pathol,2006,115(2):215-222.
[15]FURUYA S,MOCHIZUKI M,SAITO S,et al.Monitoring of QoIfungicide resistance in Plasmopara viticola populations in Japan[J].Pest Manag Sci,2010,66(11):1268-1272.
[16]BOLTON M D,RIVERA V,SECOR G.Identification of the G143Amutation associated with QoI resistance in Cercospora beticola field isolates from Michigan,United States[J].Pest Manag Sci,2013,69(1):35-39.
[17]PATEL J S,GUDMESTAD N C,MEINHARDT S,et al.Pyraclostrobin sensitivity of baseline and fungicide exposed isolates of Pyrenophora tritici-repentis[J].Crop Prot,2012,34:37-41.
[18]高杨杨,禾丽菲,李北兴,等.山东省辣椒上尖孢炭疽复合种对吡唑醚菌酯的敏感基线及吡唑醚菌酯增效配方筛选[J].农药学学报,2017,19(6):701-707.GAO Y Y,HE L F,LI B X,et al.Baseline sensitivity to pyraclostrobin of Colletotrichum acutatum species complex collected from chili in Shandong Province and the screening of synergistic formula[J].Chin J Pestic Sci,2017,19(6):701-707.
[19]毕秋艳,马志强,韩秀英,等.不同机制杀菌剂对小麦白粉病的敏感性及与三唑酮的交互抗性[J].植物保护学报,2017,44(2):331-336.BI Q Y,MA Z Q,HAN X Y,et al.Sensitivity of diverse fungicides on powdery mildew of wheat and cross resistance with triadimefon[J].J Plant Prot,2017,44(2):331-336.
[20]代玉立,甘林,阮宏椿,等.福建省玉米小斑病菌对异菌脲和吡唑醚菌酯的敏感性及其田间防效[J].农药学学报,2017,19(4):434-440.DAI Y L,GAN L,RUAN H C,et al.Sensitivity of Bipolaris maydis to iprodione and pyraclostrobin and their control efficacy against southern corn leaf blight in Fujian province[J].Chin J Pestic Sci,2017,19(4):434-440.
[21]DAI Y L,GAN L,RUAN H C,et al.Sensitivity of Cochliobolus heterostrophus to three demethylation inhibitor fungicides,propiconazole,diniconazole and prochloraz,and their efficacy against southern corn leaf blight in Fujian Province,China[J].Eur J Plant Pathol,2018,152(2):447-459.
[22]马志强,李红霞,袁章虎,等.苹果轮纹病菌对多菌灵抗药性监测初报[J].农药学学报,2000,2(3):94-96.MA Z Q,LI H X,YUAN Z H,et al.Preliminary research on detection of resistance of apple ring rot(Macrophoma kawatsukai)to carbendazim[J].Chin J Pestic Sci,2000,2(3):94-96.
[23]甘林,代玉立,滕振勇,等.福建省玉米小斑病菌对丙环唑、烯唑醇和咪鲜胺的敏感性[J].农药学学报,2016,18(2):194-200.GAN L,DAI Y L,TENG Z Y,et al.Sensitivity of Bipolaris maydis to propiconazole,diniconazole and prochloraz in Fujian Province[J].Chin J Pestic Sci,2016,18(2):194-200.
[24]林婷,时浩杰,戴德江,等.草莓炭疽病菌对吡唑醚菌酯的敏感性及旁路氧化在胶孢炭疽菌对QoIs敏感性中的作用[J].农药学学报,2015,17(5):530-537.LIN T,SHI H J,DAI D J,et al.Characterization of sensitivity of Colletotrichum gloeosporioides from strawberry to pyraclostrobin and involvement of alternative respiration in the regulation of sensitivity[J].Chin J Pestic Sci,2015,17(5):530-537.
[2]李祥栋,潘虹,陆秀娟,等.薏苡种质的主要营养组分特征及综合评价[J].中国农业科学,2018,51(5):835-842.LI X D,PAN H,LU X J,et al.Characteristics and comprehensive assessment of principal nutritional components in adlay landraces[J].Scientia Agricultura Sinica,2018,51(5):835-842.
[3]DAI Y L,GAN L,CHEN F R,et al.Leaf blight caused by Curvularia coicis on Chinese pearl barley(Coix chinensis)in Fujian Province,China[J/OL].Can J Plant Pathol,2019,41(2)[2019-01-09].https://doi.org/10.1080/07060661.2019.1567591.
[4]徐春金.薏苡主要病虫害的发生与防治[J].福建农业科技,2014(5):37-38.XU C J.Prevention and control for main pests and diseases in coix[J].Fujian Agric Sci Technol,2014(5):37-38.
[5]章霜红.薏苡叶枯病和叶斑病调查与病原鉴定[J].中国植保导刊,2012,32(6):5-7.ZHANG S H.Occurrence and identification of coix leaf blotch and coix leaf spot[J].China Plant Prot,2012,32(6):5-7.
[6]Fungicide Resistance Action Committee(FRAC).Pathogen risk list[EB/OL].[2017-03-15].http://www.frac.info/publications/downloads.
[7]FISHER N,MEUNIER B.Molecular basis of resistance to cytochrome bc1 inhibitors[J].FEMS Yeast Res,2008,8(2):183-192.
[8]BIRLA K,RIVERA-VARAS V,SECOR G A,et al.Characterization of cytochrome b from European field isolates of Cercospora beticola with quinone outside inhibitor resistance[J].Eur J Plant Pathol,2012,134(3):475-488.
[9]甘林,代玉立,杨秀娟,等.13种杀菌剂对玉米大斑病菌和弯孢霉叶斑病菌的毒力测定[J].武夷科学,2017,33:88-93.GAN L,DAI Y L,YANG X J,et al.Toxicity determination of 13fungicides to Exserohilum turcicum and Curvularia lunata[J].Wuyi Sci J,2017,33:88-93.
[10]甘林,王志纯,代玉立,等.不同杀菌剂对玉米小斑病菌的抑制作用及其防治效果[J].福建农业学报,2015,30(12):1160-1165.GAN L,WANG Z C,DAI Y L,et al.Inhibition on Bipolaria maydis and disease control on southern corn leaf blight of various fungicides[J].Fujian J Agric Sci,2015,30(12):1160-1165.
[11]FERNáNDEZ-ORTU?O D,CHEN F,SCHNABEL G.Resistance to pyraclostrobin and boscalid in Botrytis cinerea isolates from strawberry fields in the Carolinas[J].Plant Dis,2012,96(8):1198-1203.
[12]WISE K A,BRADLEY C A,PASCHE J S,et al.Resistance to QoIfungicides in Ascochyta rabiei from chickpea in the Northern Great Plains[J].Plant Dis,2009,93(5):528-536.
[13]AVENOT H,MORGAN D P,MICHAILIDES T J.Resistance to pyraclostrobin,boscalid and multiple resistance to Pristine?(pyraclostrobin+boscalid)fungicide in Alternaria alternate causing alternaria late blight of pistachios in California[J].Plant Pathol,2008,57(1):135-140.
[14]FERNáNDEZ-ORTU?O D,PéREZ-GARCíA A,LóPEZ-RUIZ F,et al.Occurrence and distribution of resistance to QoI fungicides in populations of Podosphaera fusca in south central Spain[J].Eur JPlant Pathol,2006,115(2):215-222.
[15]FURUYA S,MOCHIZUKI M,SAITO S,et al.Monitoring of QoIfungicide resistance in Plasmopara viticola populations in Japan[J].Pest Manag Sci,2010,66(11):1268-1272.
[16]BOLTON M D,RIVERA V,SECOR G.Identification of the G143Amutation associated with QoI resistance in Cercospora beticola field isolates from Michigan,United States[J].Pest Manag Sci,2013,69(1):35-39.
[17]PATEL J S,GUDMESTAD N C,MEINHARDT S,et al.Pyraclostrobin sensitivity of baseline and fungicide exposed isolates of Pyrenophora tritici-repentis[J].Crop Prot,2012,34:37-41.
[18]高杨杨,禾丽菲,李北兴,等.山东省辣椒上尖孢炭疽复合种对吡唑醚菌酯的敏感基线及吡唑醚菌酯增效配方筛选[J].农药学学报,2017,19(6):701-707.GAO Y Y,HE L F,LI B X,et al.Baseline sensitivity to pyraclostrobin of Colletotrichum acutatum species complex collected from chili in Shandong Province and the screening of synergistic formula[J].Chin J Pestic Sci,2017,19(6):701-707.
[19]毕秋艳,马志强,韩秀英,等.不同机制杀菌剂对小麦白粉病的敏感性及与三唑酮的交互抗性[J].植物保护学报,2017,44(2):331-336.BI Q Y,MA Z Q,HAN X Y,et al.Sensitivity of diverse fungicides on powdery mildew of wheat and cross resistance with triadimefon[J].J Plant Prot,2017,44(2):331-336.
[20]代玉立,甘林,阮宏椿,等.福建省玉米小斑病菌对异菌脲和吡唑醚菌酯的敏感性及其田间防效[J].农药学学报,2017,19(4):434-440.DAI Y L,GAN L,RUAN H C,et al.Sensitivity of Bipolaris maydis to iprodione and pyraclostrobin and their control efficacy against southern corn leaf blight in Fujian province[J].Chin J Pestic Sci,2017,19(4):434-440.
[21]DAI Y L,GAN L,RUAN H C,et al.Sensitivity of Cochliobolus heterostrophus to three demethylation inhibitor fungicides,propiconazole,diniconazole and prochloraz,and their efficacy against southern corn leaf blight in Fujian Province,China[J].Eur J Plant Pathol,2018,152(2):447-459.
[22]马志强,李红霞,袁章虎,等.苹果轮纹病菌对多菌灵抗药性监测初报[J].农药学学报,2000,2(3):94-96.MA Z Q,LI H X,YUAN Z H,et al.Preliminary research on detection of resistance of apple ring rot(Macrophoma kawatsukai)to carbendazim[J].Chin J Pestic Sci,2000,2(3):94-96.
[23]甘林,代玉立,滕振勇,等.福建省玉米小斑病菌对丙环唑、烯唑醇和咪鲜胺的敏感性[J].农药学学报,2016,18(2):194-200.GAN L,DAI Y L,TENG Z Y,et al.Sensitivity of Bipolaris maydis to propiconazole,diniconazole and prochloraz in Fujian Province[J].Chin J Pestic Sci,2016,18(2):194-200.
[24]林婷,时浩杰,戴德江,等.草莓炭疽病菌对吡唑醚菌酯的敏感性及旁路氧化在胶孢炭疽菌对QoIs敏感性中的作用[J].农药学学报,2015,17(5):530-537.LIN T,SHI H J,DAI D J,et al.Characterization of sensitivity of Colletotrichum gloeosporioides from strawberry to pyraclostrobin and involvement of alternative respiration in the regulation of sensitivity[J].Chin J Pestic Sci,2015,17(5):530-537.