高效液相色谱法检测三氯蔗糖的方法比较
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摘要
通过比对不同色谱柱在高效液相色谱法(High Performance Liquid Chromatography, HPLC)检测饮料中三氯蔗糖的性能,提供了不同的分析方法。采用配备示差折光检测器的高效液相色谱仪,分别使用C18反相色谱柱和Hi-Plex H色谱柱,对同一个样品进行检测。结果显示,两种方法在20～400 mg/L时,以三氯蔗糖的浓度与相应的峰面积做标准曲线,r=0.999 9,相关性良好,加标回收率为98%～103%,检出限为0.000 5 g/kg。由此得出,两种方法均能够达到国家标准的要求,并且快速、灵敏、环保,提高了高效液相色谱检测饮料中三氯蔗糖的检测效率和安全性。
By comparing the performance of different chromatographic columns in the detection of sucralose in beverages by High Performance Liquid Chromatography(HPLC), different analytical methods are provided. The same sample is detected by high performance liquid chromatography with differential refractive detector, C18 reversed phase column and Hi-Plex H column, respectively. The results show that when the two methods are 20—400 mg/L, the concentration of sucralose and the corresponding peak area are used as the standard curve, r=0.999 9. The correlation between the two methods is good. The recovery of standard addition is 98%—103%, and the detection limit is 0.000 5 g/kg. It is concluded that the two methods can meet the requirements of national standards, and are fast, sensitive and environmentally friendly, which improves the detection efficiency and safety of sucralose in beverages by high performance liquid chromatography.
By comparing the performance of different chromatographic columns in the detection of sucralose in beverages by High Performance Liquid Chromatography(HPLC), different analytical methods are provided. The same sample is detected by high performance liquid chromatography with differential refractive detector, C18 reversed phase column and Hi-Plex H column, respectively. The results show that when the two methods are 20—400 mg/L, the concentration of sucralose and the corresponding peak area are used as the standard curve, r=0.999 9. The correlation between the two methods is good. The recovery of standard addition is 98%—103%, and the detection limit is 0.000 5 g/kg. It is concluded that the two methods can meet the requirements of national standards, and are fast, sensitive and environmentally friendly, which improves the detection efficiency and safety of sucralose in beverages by high performance liquid chromatography.
引文
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[14]熊丽蓓,戴承乒,何倩琼.高效液相色谱法测定食品中三氯蔗糖含量的研究[J].上海预防医学杂志,2005(4):156-158.
[15] Valoran PH, Jeffrey S J. Determination of Sucralose in Splenda and Sugar-Free Beverage Using High-Performance AnionExchange Chromatography with Pulsed Amperometric Detection[J].Food Chem,2004,52(14):4375-4379.
[16]周玉,唐艳荣,余义,等.LC-ESI-MS/MS测定白酒中甜味剂三氯蔗糖的方法研究[J].酿酒科技,2018(12):120-123.
[17]粟晖,潘浩然,姚志湘,等.中红外光谱结合向量夹角直接定量三氯蔗糖[J].光谱学与光谱分析,2019,39(6):1742-1747.
[18] GB 22255—2014食品中三氯蔗糖(蔗糖素)的测定[S].
[19]孙晓娟,李彦博,高广慧.蒸发光散射检测器和示差检测器应用于食品中三氯蔗糖测定的比较[J].食品安全质量检测学报,2018,9(19):5114-5118.
[20]李桑,刘嘉飞,王立亚,等.高效液相色谱测定饮料中三氯蔗糖的含量的优化[J].食品安全质量检测学报,2018,9(12):2998-3003.
[21]余飞.三氯蔗糖的检测方对比研究[J].食品与发酵科技,2009,45(5):69-71.
[22]张莉.高效液相色谱法测定饮料中三氯蔗糖的含量[J].中国卫生检验杂志,2007,10(17):1784-1786.
[23]陈晓霞,游景水,杨祖伟,等.高效液相色谱法测定保健食品中三氯蔗糖的含量[J].食品安全质量检测学报,2015,5(6):1883-1888.
[2]康维民.三氯蔗糖的特性及在食品加工中的应用[J].中国食品添加剂,2002(2):78-80.
[3] Grice HC, Goldsmith LA. Sucralose-an Overview of the Toxicity Data[J].Food Chem Toxicol,2000,38(2):S1-S6.
[4] GB 2760—2014食品添加剂使用标准[S].
[5] Hunter BT. Sucralose[J].Consumer'Research Magazine,1990,73(10):8-9.
[6]陈宁,张培敏,吴述超,等.离子色谱-柱切换技术同步测定饮料中的三氯蔗糖、葡萄糖、果糖和蔗糖[J].色谱,2016(10):956-959.
[7]林立,王琳琳,钱聪,等.安培检测-离子色谱法测定食品中的三氯蔗糖[J].中国食品添加剂,2013(5):214-218.
[8] Paul J, Lobat D. Determination of Acesulfame and Sucralose in Oral Electrolyte Maintenance Solution by Liquid Chromatography[J].J AOAC,2003,86(1):79-85.
[9] Nojiri S, Nakazato M, Kasuya Y, et al. Determination of Sucralase in Foods by HPLC Using Pre-column Derivatization[J].Shokuhin Eiseigaku Zasshi,2002,43(5):289-294.
[10]甘宾宾,汤艳荣,梁川.HPLC法测定食品中三氯蔗糖[J].化工技术与开发,2008,37(8):31-32.
[11]王桂华,郭剑雄,柯华南,等.蔗糖合成甜味剂—三氯蔗糖的测定方法[J].广西轻工业,2006(5):18-19.
[12]周维义,邓小娟.高效液相色谱法分析三氯蔗糖的含量[J].食品科学,2007,28(7):360-361.
[13]粟晖,韦异,张英,等.反相高效液相色谱法分离测定三氯蔗糖[J].食品科技,2002,23(6):66-67.
[14]熊丽蓓,戴承乒,何倩琼.高效液相色谱法测定食品中三氯蔗糖含量的研究[J].上海预防医学杂志,2005(4):156-158.
[15] Valoran PH, Jeffrey S J. Determination of Sucralose in Splenda and Sugar-Free Beverage Using High-Performance AnionExchange Chromatography with Pulsed Amperometric Detection[J].Food Chem,2004,52(14):4375-4379.
[16]周玉,唐艳荣,余义,等.LC-ESI-MS/MS测定白酒中甜味剂三氯蔗糖的方法研究[J].酿酒科技,2018(12):120-123.
[17]粟晖,潘浩然,姚志湘,等.中红外光谱结合向量夹角直接定量三氯蔗糖[J].光谱学与光谱分析,2019,39(6):1742-1747.
[18] GB 22255—2014食品中三氯蔗糖(蔗糖素)的测定[S].
[19]孙晓娟,李彦博,高广慧.蒸发光散射检测器和示差检测器应用于食品中三氯蔗糖测定的比较[J].食品安全质量检测学报,2018,9(19):5114-5118.
[20]李桑,刘嘉飞,王立亚,等.高效液相色谱测定饮料中三氯蔗糖的含量的优化[J].食品安全质量检测学报,2018,9(12):2998-3003.
[21]余飞.三氯蔗糖的检测方对比研究[J].食品与发酵科技,2009,45(5):69-71.
[22]张莉.高效液相色谱法测定饮料中三氯蔗糖的含量[J].中国卫生检验杂志,2007,10(17):1784-1786.
[23]陈晓霞,游景水,杨祖伟,等.高效液相色谱法测定保健食品中三氯蔗糖的含量[J].食品安全质量检测学报,2015,5(6):1883-1888.