辣椒疫病病原菌的分离与鉴定
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摘要
[目的]明确发生在邯郸地区大名县辣椒大棚中的疫病病原菌。[方法]从辣椒大棚里采集辣椒疫病病样,对病原菌进行分离与纯化,并通过形态学观察、ITS序列分析及致病力测定,对病原菌进行鉴定。[结果]辣椒疫病病原菌为辣椒疫霉(Phytophthora capsici)。[结论]该研究为当地辣椒疫病防治提供科学的理论依据。
[Objective] The research aimed to identify the pathogen of pepper blight from Daming County of Handan area.[Method] Pepper disease samples were collected from greenhouses and the pathogen was isolated and purified.The pathogen of pepper blight was identified by comprehensive morphological characteristics,ITS DNA sequences analysis and pathogenicity test.[Result] The pathogen of pepper blight in Handan area was Phytophthora capsici.[Conclusion] The study provides a scientific theoretical basis for the prevention and control of local pepper blight.
[Objective] The research aimed to identify the pathogen of pepper blight from Daming County of Handan area.[Method] Pepper disease samples were collected from greenhouses and the pathogen was isolated and purified.The pathogen of pepper blight was identified by comprehensive morphological characteristics,ITS DNA sequences analysis and pathogenicity test.[Result] The pathogen of pepper blight in Handan area was Phytophthora capsici.[Conclusion] The study provides a scientific theoretical basis for the prevention and control of local pepper blight.
引文
[1] 姜国庆,丁和明.辣椒疫病的发生及防治技术[J].湖北植保,2014(1):26,28.
[2] KAMOUN S,FURZER O,JONES J D,et al.The top 10 oomycete pathogens in molecular plant pathology [J].Molecular plant pathology,2015,16(4):413-434.
[3] 曲丹丹,马莉莉,周海洋.辣椒疫病发病原因及综合防治措施[J].现代农业科技,2017(18):90,92.
[4] 武玉环,章彦俊,张红杰,等.辣椒疫霉菌的分离纯化及室内药剂筛选[J].北方园艺,2013(8):138-140.
[5] 郑小波.疫霉菌及其研究技术[M].北京:中国农业出版社,1997.
[6] 邱小燕,汤智鹏,张敏,等.一种适用于多数植物病原真菌的单孢分离方法[J].安徽农业科学,2011,39(9):5263-5264.
[7] 杨叔青,胡栓红,杨志刚,等.不同地区辣椒疫霉菌生理小种的鉴定和生物学特性的研究[J].华业农学报,2015,30(2):104-109.
[8] SAITOU N,NEI M.The neighbor-joining method:A new method for reconstructing phylogenetic trees[J].Molecular biology and evolution,1987,4(4):406-425.
[9] 李萍,江涛,高智谋,等.辣椒疫霉 (Phytophthora capsici) 对辣椒的致病力分化研究[J].植物病理学报,2012,42(4):431-435.
[10] 徐作珽,李林,魏道君,等.大棚辣椒疫病菌的分离培养及药剂防治[J].植物保护,1999,25(2):29-31.
[11] 张世才,熊艳,黄任中,等.重庆地区辣椒疫霉菌的分离培养及生理小种鉴定[J].植物保护,2015,41(3):183-187.
[12] 程亮,温国泉,吴永官,等.广西南瓜疫病的病原分离与鉴定[J].西南农业学报,2014,27(6):2398-2401.
[2] KAMOUN S,FURZER O,JONES J D,et al.The top 10 oomycete pathogens in molecular plant pathology [J].Molecular plant pathology,2015,16(4):413-434.
[3] 曲丹丹,马莉莉,周海洋.辣椒疫病发病原因及综合防治措施[J].现代农业科技,2017(18):90,92.
[4] 武玉环,章彦俊,张红杰,等.辣椒疫霉菌的分离纯化及室内药剂筛选[J].北方园艺,2013(8):138-140.
[5] 郑小波.疫霉菌及其研究技术[M].北京:中国农业出版社,1997.
[6] 邱小燕,汤智鹏,张敏,等.一种适用于多数植物病原真菌的单孢分离方法[J].安徽农业科学,2011,39(9):5263-5264.
[7] 杨叔青,胡栓红,杨志刚,等.不同地区辣椒疫霉菌生理小种的鉴定和生物学特性的研究[J].华业农学报,2015,30(2):104-109.
[8] SAITOU N,NEI M.The neighbor-joining method:A new method for reconstructing phylogenetic trees[J].Molecular biology and evolution,1987,4(4):406-425.
[9] 李萍,江涛,高智谋,等.辣椒疫霉 (Phytophthora capsici) 对辣椒的致病力分化研究[J].植物病理学报,2012,42(4):431-435.
[10] 徐作珽,李林,魏道君,等.大棚辣椒疫病菌的分离培养及药剂防治[J].植物保护,1999,25(2):29-31.
[11] 张世才,熊艳,黄任中,等.重庆地区辣椒疫霉菌的分离培养及生理小种鉴定[J].植物保护,2015,41(3):183-187.
[12] 程亮,温国泉,吴永官,等.广西南瓜疫病的病原分离与鉴定[J].西南农业学报,2014,27(6):2398-2401.