牧草盲蝽对七种寄主植物不同挥发物的EAG和行为反应
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摘要
【目的】研究牧草盲蝽Lygus pmtensis对寄主植物挥发物的EAG及趋向行为反应,为研发牧草盲蝽引诱剂或趋避剂策略提供科学依据。【方法】利用固相微萃取方法收集7种寄主植物包括木地肤Kochia prostrata (L.) Schrad.、灰绿藜Chenopodium glaucum、花椰菜Brassica oleracea L.var.botrytis L.、小白菜Brassica campestris L.ssp.chinensis Makino、田旋花Convolvulus arvensis L.、小藜Chenopodium serotinum L.、番茄Lycopersicon esculentum Mill.的挥发物,通过室内气相-质谱联用系统(GC-MS)进行挥发物的定性及定量分析,最终测定了9种候选特征挥发物的触角电位(EAG)反应和嗅觉趋向行为反应。【结果】7种寄主植物共鉴定到的化合物有31种,且种类和含量有较大差异,差异显著。9种候选特征挥发物均能引起牧草盲蝽雌、雄成虫的EAG反应。其中牧草盲蝽雌、雄成虫对壬醛的EAG反应绝对值最大,雌、雄虫对不同植物挥发物质EAG反应相对值均有显著差异,且对同种化合物雌虫的反应相对值均大于雄虫的反应,乙酸顺式-3-己烯酯和壬醛引发的EAG反应值差异显著;趋向行为测定中,牧草盲蝽雌虫对壬醛、金合欢烯、α-蒎烯、1-石竹烯、乙酸顺式-3-己烯酯、2-甲基-1-醇的大部分浓度的行为反应差异显著,雄虫仅对α-蒎烯、壬醛的部分浓度的行为反应差异显著。【结论】7种寄主植物的挥发物对牧草盲蝽雌、雄成虫具有不同EAG和趋向行为反应影响,候选气味化合物可以作为设计牧草盲蝽引诱剂组分进行田间害虫防控实践。
[Objectives] To identify potential attractants, or repellents, for use in the IPM of Lygus pmtensis. [Methods]The EAG and behavioral responses of L. pmtensis to volatiles from seven different host plants(Kochia prostrata(L.) Schrad.,Chenopodium glaucum, Brassica oleracea L. var. botrytis L., Convolvulus arvensis L., Brassica campestris L. ssp. chinensis Makino, Chenopodium serotinum L., Lycopersicon esculentum Mill.) were measured and compared. Volatile organic compounds from the different host plants were sampled using SPEM and then identified and quantified with GC-MS. EAG and behavioral responses to 9 candidate volatiles from different host plants were then measured and compared. [Results]Thirty-one distinct volatile compounds were isolated from the seven host plants. There was a significant difference in volatiles between species. All 9 of the candidate volatiles tested elicited EAG responses; the strongest EAG response was elicited by Nonanal. There were significant differences in the EAG responses of males and females to the volatile compounds tested;females responded to 3-Hexen-1-ol, acetate,(Z)-and Nonanal more strongly than males. Females also had significantly different behavioral responses to Nonanal, 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-,(E)-, α-Pinene, Caryophyllene,3-Hexen-1-ol, acetate,(Z)-, 1-Hexanol, 2-ethyl. [Conclusion] Male and female L. pmtensis have different EAG and behavioral responses to volatiles from different host plants. Some potential attractants for the control of L. pmtensis were identified.
[Objectives] To identify potential attractants, or repellents, for use in the IPM of Lygus pmtensis. [Methods]The EAG and behavioral responses of L. pmtensis to volatiles from seven different host plants(Kochia prostrata(L.) Schrad.,Chenopodium glaucum, Brassica oleracea L. var. botrytis L., Convolvulus arvensis L., Brassica campestris L. ssp. chinensis Makino, Chenopodium serotinum L., Lycopersicon esculentum Mill.) were measured and compared. Volatile organic compounds from the different host plants were sampled using SPEM and then identified and quantified with GC-MS. EAG and behavioral responses to 9 candidate volatiles from different host plants were then measured and compared. [Results]Thirty-one distinct volatile compounds were isolated from the seven host plants. There was a significant difference in volatiles between species. All 9 of the candidate volatiles tested elicited EAG responses; the strongest EAG response was elicited by Nonanal. There were significant differences in the EAG responses of males and females to the volatile compounds tested;females responded to 3-Hexen-1-ol, acetate,(Z)-and Nonanal more strongly than males. Females also had significantly different behavioral responses to Nonanal, 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-,(E)-, α-Pinene, Caryophyllene,3-Hexen-1-ol, acetate,(Z)-, 1-Hexanol, 2-ethyl. [Conclusion] Male and female L. pmtensis have different EAG and behavioral responses to volatiles from different host plants. Some potential attractants for the control of L. pmtensis were identified.
引文
Cai XM,Sun XL,Gao Y,Chen ZM,2008.Herbivore-induced plant volatiles:from induction to ecological functions.Chinese Journal of Applied Entomology,28(8):3969?3980.[蔡晓明,孙晓玲,董文霞,陈宗懋,2008.虫害诱导植物挥发物(HIPVs):从诱导到生态功能.生态学报,28(8):3969?3980.]
Cao N,Leng LY,Liu DC,Feng HZ,2017.Host plants and diet selection of Lygus pratenszs.China Cotton,44(3):27?29,38.[曹娜,冷凌云,刘端春,冯宏祖,2017.牧草盲蝽的寄主种类及取食选择性研究.中国棉花,44(3):27?29,38.]
Chen ZC,Su Li,Ge Feng,Su JW,2010.Electroantennogram responses of the green leaf bug Lygus lucorum Meyer-Dur to sex pheromone analogs and plant volatiles.Acta Entomologica Sinica,53(1):47?54.[陈展册,苏丽,戈峰,苏建伟,2010.绿盲蝽对性信息素类似物和植物挥发物的触角电位反应.昆虫学报,53(1):47?54.]
Dai JQ,Han SS,Du JW,2010.Progress in studies on behavioural effect of semio chemicals of host plant to insects.Journal of Environmental Entomology,32(3):407?414.[戴建青,韩诗畴,杜家纬,2010.植物挥发性信息化学物质在昆虫寄主选择行为中的作用.环境昆虫学报,32(3):407?414.]
Dai JQ,Li ZY,Chen HY,Feng Xia,Han SC,2011.Isolation and identification of plant volatiles from 3 kind of cruciferous vegetables.Guangdong Agricultural Sciences,38(22):106?108.[戴建青,李振宇,陈焕瑜,冯夏,韩诗畴,2011.3种十字花科蔬菜植物挥发性物质的分离鉴定.广东农业科学,38(22):106?108.]
Ding HJ,Wu CH,Guo YY,1995.The behavioral responses of adult cotton bollworm to volatiles of its host plants//Advances in the Biological Research of Plant Diseases and Insect Pests.Beijing:China Agricultural Science and Technology Press.193?200.[丁红建,吴才宏,郭予元,1995.棉铃虫成虫对寄主植物挥发性它感信息物的嗅觉行为研究//植物病虫害生物学研究进展.北京:中国农业科技出版社.193?200.]
Du JW,2001.Plant-insect chemical communication and its behavior control.Acta Phytophysiologica Sinica,27(3):193?200.[杜家纬,2001.植物-昆虫间的化学通讯及其行为控制.植物生理学报,27(3):193?200.]
Du YJ,Yan FS,1994.The role of plant volatiles in tritrophic interactions among phytophagous insects,their host plants and natural enemies.Acta Entomologica Sinica,37(2):233?250.[杜永均,严福顺,1994.植物挥发性次生物质在植食性昆虫、寄生植物和昆虫天敌关系中的作用机理.昆虫学报,37(2):233?250.]
Dudareva N,Negre F,Nagegowda DA,Orlova I,2006.Plant volatiles:recent advances and future perspectives.Critical Reviews in Plant Sciences,25:417?440.
Fan Hui,Jin YJ,Li JQ,Chen HJ,2004.Advances on plant volatile semiochemicals attracting herbivorous insects.Journal of Beijing Forestry University,26(3):76?81.[樊慧,金幼菊,李继泉,陈华君,2004.引诱植食性昆虫的植物挥发性信息化合物的研究进展.北京林业大学学报,26(3):76?81.]
Geng HH,2012.The behavior and its mechanisms of host preference of Apolygus lucorum(Meyer-Dur)to mungbean plants.Master thesis.Yangzhou:Yangzhou University.[耿辉辉,2012.绿盲蝽偏好绿豆的选择行为及其机制.硕士学位论文.扬州:扬州大学.]
Hu HQ,Wei XX,Cai ZJ,Wu RJ,2007.Study and application of host selectivity of Bactrocera dorsalis Hendel.Jiangxi Agricultural Sciences,19(2):68?71.[胡菡青,韦晓霞,蔡子坚,吴如健,2007.桔小实蝇寄主选择性的研究与应用.江西农业学报,19(2):68?71.]
Li HB,Wu KM,Xu Y,Yang XR,Yao J,Wang F,2007.Population dynamics of pest mirids in cotton filed in southern Xinjiang.Chinese Bulletin of Entomology,44(2):219?222.[李号宾,吴孔明,徐遥,杨秀荣,姚举,汪飞,2007.南疆棉田盲蝽类害虫种群数量动态.昆虫知识,44(2):219?222.]
Lou YG,Cheng JA,2000.Herbivore-induced plant volatiles primary characteristics,ecological functions and its release mechanism.Acta Ecologica Sinica,20(6):1097?1106.[娄永根,程家安,2000.虫害诱导的植物挥发物:基本特性、生态学功能及释放机制.生态学报,20(6):1097?1106.]
Lu YH,Qiu F,Feng HQ,Li HB,Yang ZC,Wyckhuys KAG,Wu KM,2008.Species composition and seasonal abundance of pestiferous plant bugs(Hemiptera:Miridae)on Bt cotton in China.Crop Protection,27(3/5):465?472.
Lu YH,Wu KM,Cai XM,Liu YQ,2008b.A rearing method for mirids using the green bean,Phaseolus vulgaris in the laboratory.Acta Phytophylacica Sinica,35(3):215?219.[陆宴辉,吴孔明,蔡晓明,刘仰青,2008b.利用四季豆饲养盲蝽的方法.植物保护学报,35(3):215?219.]
Lu YH,Zhang YJ,Wu KM,2008a.Host plant selection mechanisms and behavioural manipulation strategies of phytophagous insects.Acta Ecologica Sinica,28(10):5113?5122.[陆宴辉,张永军,吴孔明,2008a.植食性昆虫的寄主选择机理及行为调控策略.生态学报,28(10):5113?5122.]
Mo SS,Zhao DX,Chen Qing,2006.Advances on relationships between plant volatiles and insect behavior.Chinese Journal of Tropical Agriculture,26(6):84?89,93.[莫圣书,赵冬香,陈青,2006.植物挥发物与昆虫行为关系研究进展.热带农业科学,26(6):84?89,93.]
Pan HS,2013.Chemical communication mechanism between Apolygus lucorum(Meyer-Dur)and its fall host plants of Artemisia spp.Doctoral dissertation.Beijing:Chinese Academy of Agricultural Sciences.[潘洪生,2013.绿盲蝽与秋季蒿类寄主的化学通讯机制.博士学位论文.北京:中国农业科学院.]
Schoonhoven LM,Jermy T,Van Loon JJA,1998.Insect-Plant Biology:From Physiology to Evolution.London:Chapman and Hall.136?138.
Snodgrass GL,Gore J,Abel CA,Jackson R,2009.Acephate resistance in populations of the tarnished plant bug(Heteroptera:Miridae)from the Mississippi River Delta.Journal of Economic Entomology,102(2):699?707.
Sun P,Yang Q,Liu DC,Gou CQ,Feng HZ,2017.Taxis responses of Lygus pmtensis to 7 different host plants.XinJiang Agricultural Sciences,54(5):925?930.[孙鹏,杨琪,刘端春,苟长青,冯宏祖,2017.牧草盲蝽对七种寄主植物的趋性行为反应研究.新疆农业科学,54(5):925?930.]
Wee SL,El-Sayed AM,Gibb AR,Mitcholl V,Suckling DM,2008.Behavioural and electrophysiologieal responses of Pantotnorus cervinus(Boheman)(Coleoptera:Curculionidae)to host plant volatiles.Austral Entomology,47(1):24?31.
Williams III L,Blackmer JL,Rodriguez-Saona C,Zhu S,2010.Plant volatiles influence electrophysiological and behavioral responses of Lygus hesperus.Journal of Chemical Ecology,36(5):467?478.
Wu ZY,2006.Study on interaction between beet armyworm,Spodoptera exigua and chenopodiaceous plant.Doctoral dissertation.Hangzhou:Zhejiang University.[吴志毅,2006.甜菜夜蛾与藜科植物互作关系研究.博士学位论文.杭州:浙江大学.]
Yan FM,2011.Chemical Ecology(Second).Beijing:Science Press.237?399.[闫凤鸣,2011.化学生态学(第二版).北京:科学出版社.237?399.]
Yan SC,Zhang DD,Chi DF,2003.Advances of studies on the effects of plant volatiles on insect behavior.Chinese Journal of Applied Ecology,14(2):310?313.[严善春,张丹丹,迟德富,2003.植物挥发性物质对昆虫作用的研究进展.应用生态学报,14(2):310?313.]
Zhang JY,Lin KJ,Huang XZ,Geng T,Wei HY,Zhang YJ,2016.EAG responses and taxis selection of green plant bug Apolygus lucorum to volatiles from seven species of kinds of malvaceae plants.Chinese Journal of Biological Control,32(2):135?141.[张俊宇,林克剑,黄欣蒸,耿亭,魏洪义,张永军,2016.绿盲蝽对七种锦葵科植物挥发物的EAG和趋向行为反应.中国生物防治学报,32(2):135?141.]
Zhang LJ,Cui JZ,Li JQ,Liu CQ,Pan WL,Gao ZL,2010.Taxis responses of Lygus lucorum Meyer-Dur to volatiles of Ziziphus jujuba flowering branches with different treatments.Journal of Agricultural University of Hebei,33(4):81?84.[张立娟,崔建州,李继泉,刘春琴,潘文亮,高占林,2010.绿盲蝽对不同处理具花枣枝挥发物的趋性反应.河北农业大学学报,33(4):81?84.]
Cao N,Leng LY,Liu DC,Feng HZ,2017.Host plants and diet selection of Lygus pratenszs.China Cotton,44(3):27?29,38.[曹娜,冷凌云,刘端春,冯宏祖,2017.牧草盲蝽的寄主种类及取食选择性研究.中国棉花,44(3):27?29,38.]
Chen ZC,Su Li,Ge Feng,Su JW,2010.Electroantennogram responses of the green leaf bug Lygus lucorum Meyer-Dur to sex pheromone analogs and plant volatiles.Acta Entomologica Sinica,53(1):47?54.[陈展册,苏丽,戈峰,苏建伟,2010.绿盲蝽对性信息素类似物和植物挥发物的触角电位反应.昆虫学报,53(1):47?54.]
Dai JQ,Han SS,Du JW,2010.Progress in studies on behavioural effect of semio chemicals of host plant to insects.Journal of Environmental Entomology,32(3):407?414.[戴建青,韩诗畴,杜家纬,2010.植物挥发性信息化学物质在昆虫寄主选择行为中的作用.环境昆虫学报,32(3):407?414.]
Dai JQ,Li ZY,Chen HY,Feng Xia,Han SC,2011.Isolation and identification of plant volatiles from 3 kind of cruciferous vegetables.Guangdong Agricultural Sciences,38(22):106?108.[戴建青,李振宇,陈焕瑜,冯夏,韩诗畴,2011.3种十字花科蔬菜植物挥发性物质的分离鉴定.广东农业科学,38(22):106?108.]
Ding HJ,Wu CH,Guo YY,1995.The behavioral responses of adult cotton bollworm to volatiles of its host plants//Advances in the Biological Research of Plant Diseases and Insect Pests.Beijing:China Agricultural Science and Technology Press.193?200.[丁红建,吴才宏,郭予元,1995.棉铃虫成虫对寄主植物挥发性它感信息物的嗅觉行为研究//植物病虫害生物学研究进展.北京:中国农业科技出版社.193?200.]
Du JW,2001.Plant-insect chemical communication and its behavior control.Acta Phytophysiologica Sinica,27(3):193?200.[杜家纬,2001.植物-昆虫间的化学通讯及其行为控制.植物生理学报,27(3):193?200.]
Du YJ,Yan FS,1994.The role of plant volatiles in tritrophic interactions among phytophagous insects,their host plants and natural enemies.Acta Entomologica Sinica,37(2):233?250.[杜永均,严福顺,1994.植物挥发性次生物质在植食性昆虫、寄生植物和昆虫天敌关系中的作用机理.昆虫学报,37(2):233?250.]
Dudareva N,Negre F,Nagegowda DA,Orlova I,2006.Plant volatiles:recent advances and future perspectives.Critical Reviews in Plant Sciences,25:417?440.
Fan Hui,Jin YJ,Li JQ,Chen HJ,2004.Advances on plant volatile semiochemicals attracting herbivorous insects.Journal of Beijing Forestry University,26(3):76?81.[樊慧,金幼菊,李继泉,陈华君,2004.引诱植食性昆虫的植物挥发性信息化合物的研究进展.北京林业大学学报,26(3):76?81.]
Geng HH,2012.The behavior and its mechanisms of host preference of Apolygus lucorum(Meyer-Dur)to mungbean plants.Master thesis.Yangzhou:Yangzhou University.[耿辉辉,2012.绿盲蝽偏好绿豆的选择行为及其机制.硕士学位论文.扬州:扬州大学.]
Hu HQ,Wei XX,Cai ZJ,Wu RJ,2007.Study and application of host selectivity of Bactrocera dorsalis Hendel.Jiangxi Agricultural Sciences,19(2):68?71.[胡菡青,韦晓霞,蔡子坚,吴如健,2007.桔小实蝇寄主选择性的研究与应用.江西农业学报,19(2):68?71.]
Li HB,Wu KM,Xu Y,Yang XR,Yao J,Wang F,2007.Population dynamics of pest mirids in cotton filed in southern Xinjiang.Chinese Bulletin of Entomology,44(2):219?222.[李号宾,吴孔明,徐遥,杨秀荣,姚举,汪飞,2007.南疆棉田盲蝽类害虫种群数量动态.昆虫知识,44(2):219?222.]
Lou YG,Cheng JA,2000.Herbivore-induced plant volatiles primary characteristics,ecological functions and its release mechanism.Acta Ecologica Sinica,20(6):1097?1106.[娄永根,程家安,2000.虫害诱导的植物挥发物:基本特性、生态学功能及释放机制.生态学报,20(6):1097?1106.]
Lu YH,Qiu F,Feng HQ,Li HB,Yang ZC,Wyckhuys KAG,Wu KM,2008.Species composition and seasonal abundance of pestiferous plant bugs(Hemiptera:Miridae)on Bt cotton in China.Crop Protection,27(3/5):465?472.
Lu YH,Wu KM,Cai XM,Liu YQ,2008b.A rearing method for mirids using the green bean,Phaseolus vulgaris in the laboratory.Acta Phytophylacica Sinica,35(3):215?219.[陆宴辉,吴孔明,蔡晓明,刘仰青,2008b.利用四季豆饲养盲蝽的方法.植物保护学报,35(3):215?219.]
Lu YH,Zhang YJ,Wu KM,2008a.Host plant selection mechanisms and behavioural manipulation strategies of phytophagous insects.Acta Ecologica Sinica,28(10):5113?5122.[陆宴辉,张永军,吴孔明,2008a.植食性昆虫的寄主选择机理及行为调控策略.生态学报,28(10):5113?5122.]
Mo SS,Zhao DX,Chen Qing,2006.Advances on relationships between plant volatiles and insect behavior.Chinese Journal of Tropical Agriculture,26(6):84?89,93.[莫圣书,赵冬香,陈青,2006.植物挥发物与昆虫行为关系研究进展.热带农业科学,26(6):84?89,93.]
Pan HS,2013.Chemical communication mechanism between Apolygus lucorum(Meyer-Dur)and its fall host plants of Artemisia spp.Doctoral dissertation.Beijing:Chinese Academy of Agricultural Sciences.[潘洪生,2013.绿盲蝽与秋季蒿类寄主的化学通讯机制.博士学位论文.北京:中国农业科学院.]
Schoonhoven LM,Jermy T,Van Loon JJA,1998.Insect-Plant Biology:From Physiology to Evolution.London:Chapman and Hall.136?138.
Snodgrass GL,Gore J,Abel CA,Jackson R,2009.Acephate resistance in populations of the tarnished plant bug(Heteroptera:Miridae)from the Mississippi River Delta.Journal of Economic Entomology,102(2):699?707.
Sun P,Yang Q,Liu DC,Gou CQ,Feng HZ,2017.Taxis responses of Lygus pmtensis to 7 different host plants.XinJiang Agricultural Sciences,54(5):925?930.[孙鹏,杨琪,刘端春,苟长青,冯宏祖,2017.牧草盲蝽对七种寄主植物的趋性行为反应研究.新疆农业科学,54(5):925?930.]
Wee SL,El-Sayed AM,Gibb AR,Mitcholl V,Suckling DM,2008.Behavioural and electrophysiologieal responses of Pantotnorus cervinus(Boheman)(Coleoptera:Curculionidae)to host plant volatiles.Austral Entomology,47(1):24?31.
Williams III L,Blackmer JL,Rodriguez-Saona C,Zhu S,2010.Plant volatiles influence electrophysiological and behavioral responses of Lygus hesperus.Journal of Chemical Ecology,36(5):467?478.
Wu ZY,2006.Study on interaction between beet armyworm,Spodoptera exigua and chenopodiaceous plant.Doctoral dissertation.Hangzhou:Zhejiang University.[吴志毅,2006.甜菜夜蛾与藜科植物互作关系研究.博士学位论文.杭州:浙江大学.]
Yan FM,2011.Chemical Ecology(Second).Beijing:Science Press.237?399.[闫凤鸣,2011.化学生态学(第二版).北京:科学出版社.237?399.]
Yan SC,Zhang DD,Chi DF,2003.Advances of studies on the effects of plant volatiles on insect behavior.Chinese Journal of Applied Ecology,14(2):310?313.[严善春,张丹丹,迟德富,2003.植物挥发性物质对昆虫作用的研究进展.应用生态学报,14(2):310?313.]
Zhang JY,Lin KJ,Huang XZ,Geng T,Wei HY,Zhang YJ,2016.EAG responses and taxis selection of green plant bug Apolygus lucorum to volatiles from seven species of kinds of malvaceae plants.Chinese Journal of Biological Control,32(2):135?141.[张俊宇,林克剑,黄欣蒸,耿亭,魏洪义,张永军,2016.绿盲蝽对七种锦葵科植物挥发物的EAG和趋向行为反应.中国生物防治学报,32(2):135?141.]
Zhang LJ,Cui JZ,Li JQ,Liu CQ,Pan WL,Gao ZL,2010.Taxis responses of Lygus lucorum Meyer-Dur to volatiles of Ziziphus jujuba flowering branches with different treatments.Journal of Agricultural University of Hebei,33(4):81?84.[张立娟,崔建州,李继泉,刘春琴,潘文亮,高占林,2010.绿盲蝽对不同处理具花枣枝挥发物的趋性反应.河北农业大学学报,33(4):81?84.]