HCT值实时修正全血CRP测定结果的评估
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摘要
目的利用血细胞比容(HCT)值对全血C反应蛋白(CRP)测定结果进行实时修正,以保证结果的准确性。方法收集217例患者的血常规标本,其中37例HCT<35%、128例HCT为35%～45%,52例HCT>45%。评估XN 2800血常规CRP流水线修正前后CRP结果的可比性。结果对于HCT<35%的37例标本,未修正HCT的CRP与BNⅡ特定蛋白分析仪检测结果间平均偏差为11.77 mg/L,偏差大小与HCT值降低呈负相关,修正后CRP检测结果平均偏差为2.75 mg/L。对于HCT为35%～45%的128例标本,未修正HCT的CRP与BNⅡ特定蛋白分析仪检测结果间平均偏差为2.79mg/L,修正后CRP检测结果平均偏差为2.77mg/L。对于HCT>45%的52例标本,未修正HCT的CRP与BNⅡ特定蛋白分析仪检测结果间平均偏差为-11.84 mg/L,偏差大小与HCT值升高呈正相关,修正后CRP检测结果平均偏差为-1.35 mg/L。结论对HCT<35%和>45%的标本,宜采用HCT值实时修正CRP结果,使检测结果更为准确。
Objective To evaluate the real-time correction of whole blood C-reactive protein(CRP)determination using hematocrit(HCT) value in order to ensure the accuracy of determination results.Methods A total of 217 blood samples were collected.HCT<35% was found in 37 cases,HCT 35%-45% in 128 cases,and HCT>45% in 52 cases.The comparability of CRP levels before and after correcting in XN 2800 was evaluated.Results The mean deviation between uncorrected level and target CRP level by BN Ⅱ specific protein analyzer was 11.77 mg/L in 37 cases with HCT<35%,and the deviation was negatively correlated with the decrease of HCT,while the mean deviation between corrected and target CRP levels was 2.75 mg/L.In 128 cases with HCT 35%-45%,the mean deviation between uncorrected and target CRP levels was 2.79 mg/L,while the mean deviation between corrected and target CRP levels was 2.77 mg/L.In 52 cases with HCT>45%,the mean deviation between uncorrected and target CRP levels was-11.84 mg/L,and the deviation was positively correlated with the increase of HCT,while the mean deviation between corrected and target CRP levels was-1.35 mg/L.Conclusions As to blood samples with HCT<35% and HCT>45%,real-time correction of whole CRP value determination using HCT value can make the determination results more accurate.
Objective To evaluate the real-time correction of whole blood C-reactive protein(CRP)determination using hematocrit(HCT) value in order to ensure the accuracy of determination results.Methods A total of 217 blood samples were collected.HCT<35% was found in 37 cases,HCT 35%-45% in 128 cases,and HCT>45% in 52 cases.The comparability of CRP levels before and after correcting in XN 2800 was evaluated.Results The mean deviation between uncorrected level and target CRP level by BN Ⅱ specific protein analyzer was 11.77 mg/L in 37 cases with HCT<35%,and the deviation was negatively correlated with the decrease of HCT,while the mean deviation between corrected and target CRP levels was 2.75 mg/L.In 128 cases with HCT 35%-45%,the mean deviation between uncorrected and target CRP levels was 2.79 mg/L,while the mean deviation between corrected and target CRP levels was 2.77 mg/L.In 52 cases with HCT>45%,the mean deviation between uncorrected and target CRP levels was-11.84 mg/L,and the deviation was positively correlated with the increase of HCT,while the mean deviation between corrected and target CRP levels was-1.35 mg/L.Conclusions As to blood samples with HCT<35% and HCT>45%,real-time correction of whole CRP value determination using HCT value can make the determination results more accurate.
引文
[1]沈逸辛,张剑峰,许中.普门特定蛋白分析仪检测全血C反应蛋白性能验证[J].中国血液流变学杂志,2015,25(4):504-505.
[2]隆维东,李坚,刘万彬.不同红细胞压积对全血CRP测定的影响及校正措施[J].国际检验医学杂志,2012,33(1):107-109.
[2]隆维东,李坚,刘万彬.不同红细胞压积对全血CRP测定的影响及校正措施[J].国际检验医学杂志,2012,33(1):107-109.