乳鸽早期生长发育及氨基酸营养调控研究
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摘要
乳鸽出壳后依赖于亲鸽鸽乳的哺育,严重限制母鸽产雏数,制约鸽业发展。文中基于前人对乳鸽生长发育特点的研究,通过测定鸽乳中的氨基酸组成和含量,分析出壳后乳鸽早期小肠氨基酸转运载体的时空表达规律,揭示乳鸽肠道氨基酸转运的分子机制。在此基础上,采用胚蛋给养的技术方法,探讨碱性氨基酸对乳鸽早期生长发育的营养调控作用。
during the immediately post-hatch period,young pigeons( squabs) grow depend on the crop milk from parental pigeon,which severely limits the production of the female pigeon,and restricts the development of pigeon industry. Based on the previous studies on the growth development characteristics of squab,this article revealed the molecular mechanism of amino acid transport in the intestine by determining the amino acid composition and content in crop milk and analyzing the temporal and spatial expression of amino acid transporters in small intestine of squabs during the early post-hatch period. Furthermore,in ovo injection was adopted to explore the nutritional manipulation of basic amino acids on early growth development of squab.
during the immediately post-hatch period,young pigeons( squabs) grow depend on the crop milk from parental pigeon,which severely limits the production of the female pigeon,and restricts the development of pigeon industry. Based on the previous studies on the growth development characteristics of squab,this article revealed the molecular mechanism of amino acid transport in the intestine by determining the amino acid composition and content in crop milk and analyzing the temporal and spatial expression of amino acid transporters in small intestine of squabs during the early post-hatch period. Furthermore,in ovo injection was adopted to explore the nutritional manipulation of basic amino acids on early growth development of squab.
引文
[1]陈益填.我国肉鸽业现状、投资效益与趋势[J].猪业观察,2012(8):8-9.
[2] Sales J,Janssens G P J.Nutrition of the domestic pigeon(Columba livia domestica)[J]. Worlds Poultry Science Journal,2003,59(2):221-232.
[3]杜正智,沈禹颐,李军祖,等.乳鸽生长发育特点研究[J].甘肃畜牧兽医,1993(1):14-15.
[4] Darwati S,Martojo H,Sumantri C,et al. Productivity,repeatability of productive and reproductive traits of local pigeon[J].Public Administration Quarterly,2010,70(1):268-274.
[5] Gao C Q,Yang J X,Chen M X,et al.Growth curves and agerelated changes in carcass characteristics,organs,serum parameters,and intestinal transporter gene expression in domestic pigeon(Columba livia)[J]. Poultry Science,2016,95(4):867-877.
[6] Khargharia G,Goswami R N,Das D.Carcass characteristics of domesticated pigeon squabs of Assam[J]. Indian Veterinary Journal,2002,79(4):400-401.
[7] Chen M X,Li X G,Yang J X,et al.The growth of embryo and gene expression of nutrient transporters in the small intestine of domestic pigeon(Columba livia)[J]. Journal of Zhejiang University-Science B,2015,16(6):511-523.
[8] Gillespie M J,Haring V R,Mc Coll K A,et al.Histological and global gene expression analysis of the ‘lactating’pigeon crop[J].BMC Genomics,2011,12(1):452.
[9] Gillespie M J,Stanley D,Chen H L,et al.Functional similarities between pigeon'milk'and mammalian milk:induction of immune gene expression and modification of the microbiota[J].Plos One,2012,7(10):e48363.
[10] Vandeputte-Poma J.Feeding,growth and metabolism of the pigeon,Columba livia domestica:duration and role of crop milk feeding[J]. Journal of Comparative Physiology,1980,135(2):97-99.
[11]王萨仁图雅,宝华,徐桂杰.鸽乳成分分析及鸽乳人工哺育技术的研究进展[J].当代畜禽养殖业,2013(4):7-9.
[12]曾秋凤,张彬,张在伟.自然鸽乳的常规营养分析[J].中国家禽,2003,25(21):12-14.
[13]何志华,丁家桐,沈曼曼,等.乳鸽生长及嗉囊乳营养水平分析[J].国外畜牧学:猪与禽,2012,32(2):48-50.
[14] Miska K B,Fetterer R H,Wong E A.The mRNA expression of amino acid transporters,aminopeptidase N,and the di-and tripeptide transporter PepT1 in the embryo of the domestic chicken(Gallus gallus)shows developmental regulation[J].Poultry Science,2014,93(9):2262-2270.
[15] Wu G Y. Amino acids:Metabolism,functions,and nutrition[J].Amino Acids,2009,37(1):1-17.
[16] Zhang X Y,Zhang N N,Wan X P,et al.Gene expression of amino acid transporter in pigeon(Columbia livia)intestine during post-hatch development and its correlation with amino acid in pigeon milk[J].Poultry Science,2016.DOI:10.3382/ps/pew320.
[17] Mondloch C J,Timberlake W. The effect of parental food supply on parental feeding and squab growth in pigeons Columbia livia[J].Ethology,1991,88(3):236-248.
[18] Uni Z,Noy Y,Sklan D.Posthatch development of small intestinal function in the poult[J]. Poultry Science,1999,78(2):215-222.
[19] Dong X Y,Wang Y M,Dai L,et al.Posthatch development of intestinal morphology and digestive enzyme activities in domestic pigeons(Columba livia)[J].Poultry Science,2012,91(8):1886-1892.
[20] Dong X Y,Wang Y M,Yuan C,et al.The ontogeny of nutrient transporter and digestive enzyme gene expression in domestic pigeon(Columba livia)intestine and yolk sac membrane during pre-and posthatch development[J]. Poultry Science,2012,91(8):1974-1982.
[21] Xie P,Zhang A T,Wang C,et al.Molecular cloning,characterization,and expression analysis of fatty acid translocase(FAT/CD36)in the pigeon(Columba livia domestica)[J].Poultry Science,2012,91(7):1670-1679.
[22]贾良梁,严从天,丁家桐,等.三种神经递质类氨基酸对乳鸽生长性能的影响[J].国外畜牧学(猪与禽),2015(1):73-75.
[23] Zhang X Y,Li L L,Miao L P,et al.Effects of in ovo feeding of cationic amino acids on hatchability,hatch weights,and organ developments in domestic pigeon squabs(Columba livia)[J].Poult Sci,2017,97(1):110-117.
[2] Sales J,Janssens G P J.Nutrition of the domestic pigeon(Columba livia domestica)[J]. Worlds Poultry Science Journal,2003,59(2):221-232.
[3]杜正智,沈禹颐,李军祖,等.乳鸽生长发育特点研究[J].甘肃畜牧兽医,1993(1):14-15.
[4] Darwati S,Martojo H,Sumantri C,et al. Productivity,repeatability of productive and reproductive traits of local pigeon[J].Public Administration Quarterly,2010,70(1):268-274.
[5] Gao C Q,Yang J X,Chen M X,et al.Growth curves and agerelated changes in carcass characteristics,organs,serum parameters,and intestinal transporter gene expression in domestic pigeon(Columba livia)[J]. Poultry Science,2016,95(4):867-877.
[6] Khargharia G,Goswami R N,Das D.Carcass characteristics of domesticated pigeon squabs of Assam[J]. Indian Veterinary Journal,2002,79(4):400-401.
[7] Chen M X,Li X G,Yang J X,et al.The growth of embryo and gene expression of nutrient transporters in the small intestine of domestic pigeon(Columba livia)[J]. Journal of Zhejiang University-Science B,2015,16(6):511-523.
[8] Gillespie M J,Haring V R,Mc Coll K A,et al.Histological and global gene expression analysis of the ‘lactating’pigeon crop[J].BMC Genomics,2011,12(1):452.
[9] Gillespie M J,Stanley D,Chen H L,et al.Functional similarities between pigeon'milk'and mammalian milk:induction of immune gene expression and modification of the microbiota[J].Plos One,2012,7(10):e48363.
[10] Vandeputte-Poma J.Feeding,growth and metabolism of the pigeon,Columba livia domestica:duration and role of crop milk feeding[J]. Journal of Comparative Physiology,1980,135(2):97-99.
[11]王萨仁图雅,宝华,徐桂杰.鸽乳成分分析及鸽乳人工哺育技术的研究进展[J].当代畜禽养殖业,2013(4):7-9.
[12]曾秋凤,张彬,张在伟.自然鸽乳的常规营养分析[J].中国家禽,2003,25(21):12-14.
[13]何志华,丁家桐,沈曼曼,等.乳鸽生长及嗉囊乳营养水平分析[J].国外畜牧学:猪与禽,2012,32(2):48-50.
[14] Miska K B,Fetterer R H,Wong E A.The mRNA expression of amino acid transporters,aminopeptidase N,and the di-and tripeptide transporter PepT1 in the embryo of the domestic chicken(Gallus gallus)shows developmental regulation[J].Poultry Science,2014,93(9):2262-2270.
[15] Wu G Y. Amino acids:Metabolism,functions,and nutrition[J].Amino Acids,2009,37(1):1-17.
[16] Zhang X Y,Zhang N N,Wan X P,et al.Gene expression of amino acid transporter in pigeon(Columbia livia)intestine during post-hatch development and its correlation with amino acid in pigeon milk[J].Poultry Science,2016.DOI:10.3382/ps/pew320.
[17] Mondloch C J,Timberlake W. The effect of parental food supply on parental feeding and squab growth in pigeons Columbia livia[J].Ethology,1991,88(3):236-248.
[18] Uni Z,Noy Y,Sklan D.Posthatch development of small intestinal function in the poult[J]. Poultry Science,1999,78(2):215-222.
[19] Dong X Y,Wang Y M,Dai L,et al.Posthatch development of intestinal morphology and digestive enzyme activities in domestic pigeons(Columba livia)[J].Poultry Science,2012,91(8):1886-1892.
[20] Dong X Y,Wang Y M,Yuan C,et al.The ontogeny of nutrient transporter and digestive enzyme gene expression in domestic pigeon(Columba livia)intestine and yolk sac membrane during pre-and posthatch development[J]. Poultry Science,2012,91(8):1974-1982.
[21] Xie P,Zhang A T,Wang C,et al.Molecular cloning,characterization,and expression analysis of fatty acid translocase(FAT/CD36)in the pigeon(Columba livia domestica)[J].Poultry Science,2012,91(7):1670-1679.
[22]贾良梁,严从天,丁家桐,等.三种神经递质类氨基酸对乳鸽生长性能的影响[J].国外畜牧学(猪与禽),2015(1):73-75.
[23] Zhang X Y,Li L L,Miao L P,et al.Effects of in ovo feeding of cationic amino acids on hatchability,hatch weights,and organ developments in domestic pigeon squabs(Columba livia)[J].Poult Sci,2017,97(1):110-117.