基于SNP和SSR标记的小麦品质性状的QTL定位
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摘要
为给小麦品质性状的标记辅助选择提供备选分子标记,并进一步定位和克隆小麦部分品质相关性状的QTL,本研究利用普通小麦Heyne×Lakin杂交F2代单粒传获得的145个F8代重组自交系(Recombinant inbred line, RIL)群体,构建了含有2 082对标记(1 980对SNP标记和102对SSR标记)、总长度为2 120.13 cM的遗传连锁图谱,并利用该图谱对小麦连续两年的品质性状(蛋白质含量,湿面筋含量,淀粉含量和Zeleny沉降值)进行了QTL分析。结果表明,共检测出11个QTL,其中,蛋白质含量有2个QTL,位于2A和5A染色体上,可解释的表型变异率6.94%～12.55%;湿面筋含量QTL有1个,位于5A染色体上,可解释的表型变异率8.40%～12.96%;淀粉含量QTL有3个,位于2A和5A染色体上,可解释的表型变异率9.78%～11.23%;Zeleny沉降值QTL有5个,位于2A、5A和7D染色体上,可解释的表型变异率4.75%～20.15%。其中5A染色体上存在这些品质性状QTL富集区,同时具有"一因多效"QTL位点。这个区段的发现,为小麦品质育种提供了参考依据。
In order to provide candidate molecular markers for marker-assisted selection of wheat quality traits and further locate and clone QTLs for some quality-related traits in wheat, 145 F8-generation recombinant selfcrosses obtained from single wheat granules of common wheat Heyne ×Lakin hybrid F2 generation were used in this study. Recombinant inbred line(RIL) population, constructed a genetic linkage map containing 2 082 pairs of markers(1 980 pairs of SNP markers and 102 pairs of SSR markers) with a total length of 2 120.13 cM, and using this map for wheat for two consecutive years QTL analysis was performed on quality traits(protein content, wet gluten content, starch content and Zeleny sedimentation value). The results showed that eleven additive QTLs for quality related traits were detected, including two of them for protein content were mapped on chromosomes 2 A and 5 A, explaining 6.94%～12.55% of the phenotypic variances; one QTL for wet gluten content were located on chro-mosomes 5 A, accounting for 8.40%～12.96% of the phenotypic variances; three QTLs for starch content were mapped on chromosomes 2 A and 5 A, explaining the phenotypic variation of 9.78%～11.23%; five QTLs for Zeleny sedimentation value were located on chromosomes 2 A, 5 A and 7 D, accounting for 4.75%～20.15% of the phenotypic variances. Among them, there are QTL enrichment regions of these quality traits on chromosome 5 A, and there is a "multiple effect" QTL locus. The discovery provide reference for wheat quality breeding.
In order to provide candidate molecular markers for marker-assisted selection of wheat quality traits and further locate and clone QTLs for some quality-related traits in wheat, 145 F8-generation recombinant selfcrosses obtained from single wheat granules of common wheat Heyne ×Lakin hybrid F2 generation were used in this study. Recombinant inbred line(RIL) population, constructed a genetic linkage map containing 2 082 pairs of markers(1 980 pairs of SNP markers and 102 pairs of SSR markers) with a total length of 2 120.13 cM, and using this map for wheat for two consecutive years QTL analysis was performed on quality traits(protein content, wet gluten content, starch content and Zeleny sedimentation value). The results showed that eleven additive QTLs for quality related traits were detected, including two of them for protein content were mapped on chromosomes 2 A and 5 A, explaining 6.94%～12.55% of the phenotypic variances; one QTL for wet gluten content were located on chro-mosomes 5 A, accounting for 8.40%～12.96% of the phenotypic variances; three QTLs for starch content were mapped on chromosomes 2 A and 5 A, explaining the phenotypic variation of 9.78%～11.23%; five QTLs for Zeleny sedimentation value were located on chromosomes 2 A, 5 A and 7 D, accounting for 4.75%～20.15% of the phenotypic variances. Among them, there are QTL enrichment regions of these quality traits on chromosome 5 A, and there is a "multiple effect" QTL locus. The discovery provide reference for wheat quality breeding.
引文
Blanco A.,De Giovanni C.,L addomada B.,Sciancalepore A.,Simeone R.,Devos K.M.,and Gale M.D.,1996,Quantitative trait loci influencing grain protein content in tetraploid wheats,Plant Breeding,115(5):310-316
Blanco A.,Pasqualone A.,Troccoli A.,Di F.N.,and Simeone R.,2002,Detection of grain protein content QTLs across environments in tetraploid wheats,Plant Mol.Biol.,48(5-6):615-623
Guo L.J.,2016,The QTL mapping and effects analysis of wheat grain yield and quality traits based on SRAP and SSR,Thesis for M.S.,Northwest Agricultural and Forestry University,Supervisor:Zhao H.X.,pp.31-34(郭利建,2016,基于SRAP和SSR标记的小麦产量和品质相关性状的QTL定位及效应分析,硕士学位论文,西北农林科技大学,导师:赵惠贤,pp.31-34)
He Z.H.,Yan Y.M.,Zhang Q.S.,Zhang Y.,Xia X.C.,Zhang Y.,Wang D.S.,Xia L.Q.,Hu Y.K.,Cai M.H.,Chen X.M.,and Yan J.,and Zhou Y.,2006,Establishment of quality evaluation system and utilization of molecular methods for the improvement of Chinese wheat quality,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),39(6):1091-1101(何中虎,晏月明,庄巧生,张艳,夏先春,张勇,王德森,夏兰芹,胡英考,蔡民华,陈新民,阎俊,周阳,2006,中国小麦品种品质评价体系建立与分子改良技术研究,中国农业科学,39(6):1091-1101)
Huang X.Q.,Cloutier S.,Lycar L.,Radovanovic N.,Humphreys D.G.,Noll J.S.,Somers D.J.,and Brown P.D.,2006,Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats(Triticum aestivum L.),Theor.Appl.Genet.,113(4):753-766
Ku L.X.,Zhang J.,Zhang J.C.,Guo S.L.,Liu H.Y.,Zhao R.F.,Yan Q.X.,and Chen Y.H.,2012,Genetic dissection of leaf area by jointing two F2:3populations in maize(Zea mays L.),Plant Breeding,131(5):591-599
Li J.,Cui F.,Ding A.M.,Zhao C.H.,Wang X.Q.,Wang L.,Bao Y.G.,Qi X.L.,Li X.F.,Gao J.R.,Feng D.S.,and Wang H.G.,2012,QTL detection of seven quality traits in wheat using two related recombinant inbred line populations,Euphytica,183(2):207-226
Li W.H.,Liu W.,Liu L.,and Cao L.P.,2005,A review of QTLresearch for wheat quality traits,Shihezi Daxue Xuebao(Journal of Shihezi University(Natural Science)),23(3):389-392(李卫华,刘伟,刘丽,曹连蒲,2005,小麦品质性状的QTL定位研究进展,石河子大学学报(自然科学版),23(3):389-392)
Ma J.,Zhang C.Y.,Yan G.J.,and Liu C.J.,2012,Identification of QTLs conferring agronomic and quality traits in hexaploid wheat,J.Integr.Agr.,11(9):1399-1408
Mccartney C.A.,Somers D.J.,Humphreys D.G.,Lukow O.,Ames N.,Noll J.,Cloutier S.,and Mccallum B.D.,2005,Mapping quantitative traits loci controlling agronomic traits in the spring wheat cross RL4452×'AC Domain',Genome,48(5):870-883
Mccartney C.A.,Somers D.J.,Lukow O.,Ames N.,Noll J.,Cloutier S.,Humphreys D.G.,and McCallum B.D.,2006,QTL analysis of quality traits in the spring wheat cross RL4452×'AC Domain',Plant Breeding,125(6):565-575
Stuber C.W.,Edwards M.D.,and Wendel J.F.,1987,Molecular maker-facilitated investigations of quantitative traits loci in maize.Ⅱ.Factors influencing yield and its component traits,Crop Sci.,27(4):639-648
Sun Z.P.,Liu T.X.,Zuo X.Y,Zhao J.C.,Wang Z.H.,and Li C.L,2017,QTL mapping of spike-related traits in common wheat,Mailei Zuowu Xuebao(Journal of Triticeae Crops),37(4):452-457(孙中沛,刘天相,左希亚,赵璟琛,王中华,李春莲,2017,普通小麦穗部性状QTL分析,麦类作物学报,37(4):452-457)
Tang H.,Yan J.B.,Huang Y.Q.,Zheng Y.L.,and Li J.S.,2005,QTL mapping of five agronomic traits in maize,Yichuan Xuebao(Acta Genetica Sinica),32(2):203-209(汤华,严建兵,黄益勤,郑用琏,李建生,2005,玉米5个农艺性状的QTL定位,遗传学报,32(2):203-209)
Wu Y.P.,Zhang Y.L.,Xiao Y.G.,Yan J.,Zhang Y.,Zhang X.K.,Zhang L.M.,Xia X.C.,and He Z.H.,2008,QTL mapping for important quality traits in common wheat,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),41(2):331-339(吴云鹏,张业伦,肖永贵,阎俊,张勇,张晓科,张利民,夏先春,何中虎,2008,小麦重要品质性状的QTL定位,中国农业科学,41(2):331-339)
Yang L.,Wu Q.X.,Shao H.,Ran C.F.,Yu J.,Li L.Q.,and Li X.J.,2013,QTL mapping for grain quality traits in wheat,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),33(8):1574-1583(杨林,吴青霞,邵慧,冉从福,余静,李立群,李学军,2013,小麦籽粒品质性状的QTL分析,西北植物学报,33(8):1574-1583)
Zanetti S.,Winzeler M.,Feuillet C.,Keller B.,and Messmer M.,2010,Genetic analysis of bread-making quality in wheat and spelt,Plant Breeding,120(1):13-19
Blanco A.,Pasqualone A.,Troccoli A.,Di F.N.,and Simeone R.,2002,Detection of grain protein content QTLs across environments in tetraploid wheats,Plant Mol.Biol.,48(5-6):615-623
Guo L.J.,2016,The QTL mapping and effects analysis of wheat grain yield and quality traits based on SRAP and SSR,Thesis for M.S.,Northwest Agricultural and Forestry University,Supervisor:Zhao H.X.,pp.31-34(郭利建,2016,基于SRAP和SSR标记的小麦产量和品质相关性状的QTL定位及效应分析,硕士学位论文,西北农林科技大学,导师:赵惠贤,pp.31-34)
He Z.H.,Yan Y.M.,Zhang Q.S.,Zhang Y.,Xia X.C.,Zhang Y.,Wang D.S.,Xia L.Q.,Hu Y.K.,Cai M.H.,Chen X.M.,and Yan J.,and Zhou Y.,2006,Establishment of quality evaluation system and utilization of molecular methods for the improvement of Chinese wheat quality,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),39(6):1091-1101(何中虎,晏月明,庄巧生,张艳,夏先春,张勇,王德森,夏兰芹,胡英考,蔡民华,陈新民,阎俊,周阳,2006,中国小麦品种品质评价体系建立与分子改良技术研究,中国农业科学,39(6):1091-1101)
Huang X.Q.,Cloutier S.,Lycar L.,Radovanovic N.,Humphreys D.G.,Noll J.S.,Somers D.J.,and Brown P.D.,2006,Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats(Triticum aestivum L.),Theor.Appl.Genet.,113(4):753-766
Ku L.X.,Zhang J.,Zhang J.C.,Guo S.L.,Liu H.Y.,Zhao R.F.,Yan Q.X.,and Chen Y.H.,2012,Genetic dissection of leaf area by jointing two F2:3populations in maize(Zea mays L.),Plant Breeding,131(5):591-599
Li J.,Cui F.,Ding A.M.,Zhao C.H.,Wang X.Q.,Wang L.,Bao Y.G.,Qi X.L.,Li X.F.,Gao J.R.,Feng D.S.,and Wang H.G.,2012,QTL detection of seven quality traits in wheat using two related recombinant inbred line populations,Euphytica,183(2):207-226
Li W.H.,Liu W.,Liu L.,and Cao L.P.,2005,A review of QTLresearch for wheat quality traits,Shihezi Daxue Xuebao(Journal of Shihezi University(Natural Science)),23(3):389-392(李卫华,刘伟,刘丽,曹连蒲,2005,小麦品质性状的QTL定位研究进展,石河子大学学报(自然科学版),23(3):389-392)
Ma J.,Zhang C.Y.,Yan G.J.,and Liu C.J.,2012,Identification of QTLs conferring agronomic and quality traits in hexaploid wheat,J.Integr.Agr.,11(9):1399-1408
Mccartney C.A.,Somers D.J.,Humphreys D.G.,Lukow O.,Ames N.,Noll J.,Cloutier S.,and Mccallum B.D.,2005,Mapping quantitative traits loci controlling agronomic traits in the spring wheat cross RL4452×'AC Domain',Genome,48(5):870-883
Mccartney C.A.,Somers D.J.,Lukow O.,Ames N.,Noll J.,Cloutier S.,Humphreys D.G.,and McCallum B.D.,2006,QTL analysis of quality traits in the spring wheat cross RL4452×'AC Domain',Plant Breeding,125(6):565-575
Stuber C.W.,Edwards M.D.,and Wendel J.F.,1987,Molecular maker-facilitated investigations of quantitative traits loci in maize.Ⅱ.Factors influencing yield and its component traits,Crop Sci.,27(4):639-648
Sun Z.P.,Liu T.X.,Zuo X.Y,Zhao J.C.,Wang Z.H.,and Li C.L,2017,QTL mapping of spike-related traits in common wheat,Mailei Zuowu Xuebao(Journal of Triticeae Crops),37(4):452-457(孙中沛,刘天相,左希亚,赵璟琛,王中华,李春莲,2017,普通小麦穗部性状QTL分析,麦类作物学报,37(4):452-457)
Tang H.,Yan J.B.,Huang Y.Q.,Zheng Y.L.,and Li J.S.,2005,QTL mapping of five agronomic traits in maize,Yichuan Xuebao(Acta Genetica Sinica),32(2):203-209(汤华,严建兵,黄益勤,郑用琏,李建生,2005,玉米5个农艺性状的QTL定位,遗传学报,32(2):203-209)
Wu Y.P.,Zhang Y.L.,Xiao Y.G.,Yan J.,Zhang Y.,Zhang X.K.,Zhang L.M.,Xia X.C.,and He Z.H.,2008,QTL mapping for important quality traits in common wheat,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),41(2):331-339(吴云鹏,张业伦,肖永贵,阎俊,张勇,张晓科,张利民,夏先春,何中虎,2008,小麦重要品质性状的QTL定位,中国农业科学,41(2):331-339)
Yang L.,Wu Q.X.,Shao H.,Ran C.F.,Yu J.,Li L.Q.,and Li X.J.,2013,QTL mapping for grain quality traits in wheat,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),33(8):1574-1583(杨林,吴青霞,邵慧,冉从福,余静,李立群,李学军,2013,小麦籽粒品质性状的QTL分析,西北植物学报,33(8):1574-1583)
Zanetti S.,Winzeler M.,Feuillet C.,Keller B.,and Messmer M.,2010,Genetic analysis of bread-making quality in wheat and spelt,Plant Breeding,120(1):13-19