花生种子及花生植株各部位白藜芦醇含量的测定
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摘要
为准确测定白藜芦醇在花生种子及花生植株各部位中的含量,通过超声萃取和高效液相色谱法测定花生种子及花生植株各部位的白藜芦醇含量。结果表明,花生植株根部中白藜芦醇含量最高,达到629.487μg/g,花生种子中含量极低,不超过2μg/g;种子发芽处理后,白藜芦醇含量最高,达到28.041μg/g,满足工业提取对白藜芦醇含量的要求,说明花生种子处理后及花生植株对白藜芦醇来源有较大的经济价值。
In order to accurately determine the content of resveratrol in peanut seeds and parts of peanut plants, ultrasonic extraction and high performance liquid chromatography were applied in the experiment.The results showed that the content of resveratrol in the root of peanut plant was the highest, reaching629.487 μg/g, and the content of resveratrol in peanut seed was very low, not exceeding 2 μg/g. After seed germination treatment, the highest content of resveratrol was 28.041 μg/g, which met the requirements of industrial extraction for resveratrol content. As a result, the treatment of peanut seeds and peanut plants have great economic value for resveratrol sources.
In order to accurately determine the content of resveratrol in peanut seeds and parts of peanut plants, ultrasonic extraction and high performance liquid chromatography were applied in the experiment.The results showed that the content of resveratrol in the root of peanut plant was the highest, reaching629.487 μg/g, and the content of resveratrol in peanut seed was very low, not exceeding 2 μg/g. After seed germination treatment, the highest content of resveratrol was 28.041 μg/g, which met the requirements of industrial extraction for resveratrol content. As a result, the treatment of peanut seeds and peanut plants have great economic value for resveratrol sources.
引文
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[9]华芳,施伶俐,唐晓敏.葡萄皮籽提取物中白藜芦醇的含量测定及其凝胶剂的制备工艺[J].盐城工学院学报(自然科学版),2017,30(4):54-57.
[10]刘一,李媛,赵立波,等.4种白藜芦醇类化合物的电喷雾多级质谱裂解规律研究[J].中国药房,2013,24(01):33-36.
[11]张煜,胡君萍,韩玫,等.HPLC法测定花生不同部位中白藜芦醇的含量[J].新疆医科大学学报,2003,(05):440-441.
[12]周晓明,郭春苗,樊丁宇,等.HPLC法测定不同品种葡萄及葡萄干白藜芦醇含量[J].新疆农业科学,2013,50(12):2223-2229.
[13]刘丹,汤海峰,张三奇,等.虎杖中有效成分提取方法的研究[J].中成药,2007,29(4):516-521.
[2]吕春晖,高原,王玉姝.白藜芦醇的生理作用及其在制剂中应用[J].生物技术世界,2016,(5):203-203.
[3]Siemann E H,Creasy L L.Concentration of the Phytoalexin Resveratrol in Wine[J].Am j enol vitic,1992,43(2):401-405.
[4]Vidavalur R,Otani H,Singal P K,et al.Significance of wine and resveratrol in cardiovascular disease:French paradox revisited[J].Experimental&Clinical Cardiology,2006,11(3):217.
[5]夏晨,金晶,苟晓莉,等.白藜芦醇四甲氧基衍生物提高耐药人乳腺癌细胞MCF-7/DOX对阿霉素的敏感性[J].中药药理与临床,2014,(6):45-48.
[6]Garvin S,Llinger K,Dabrosin C.Resveratrol induces apoptosis and inhibits angiogenesis in human breast cancer xenografts in vivo[J].Cancer Letters,2006,231(1):0-122.
[7]KUNDU J K,SURH Y J.Cancer chemopreventive and therapeutic potential of resveratrol:Mechanistic perspectives[J].Cancer Letters,2008,269(2,SI):243-261.
[8]SIGNORELLI P,GHIDONI R.Resveratrol as an anticancer nutrient:molecular basis,open questions and promises[J].Journal of Nutritional Biochemistry,2005,16(8):449-466.
[9]华芳,施伶俐,唐晓敏.葡萄皮籽提取物中白藜芦醇的含量测定及其凝胶剂的制备工艺[J].盐城工学院学报(自然科学版),2017,30(4):54-57.
[10]刘一,李媛,赵立波,等.4种白藜芦醇类化合物的电喷雾多级质谱裂解规律研究[J].中国药房,2013,24(01):33-36.
[11]张煜,胡君萍,韩玫,等.HPLC法测定花生不同部位中白藜芦醇的含量[J].新疆医科大学学报,2003,(05):440-441.
[12]周晓明,郭春苗,樊丁宇,等.HPLC法测定不同品种葡萄及葡萄干白藜芦醇含量[J].新疆农业科学,2013,50(12):2223-2229.
[13]刘丹,汤海峰,张三奇,等.虎杖中有效成分提取方法的研究[J].中成药,2007,29(4):516-521.