芸薹属蔬菜再生体系研究进展
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摘要
再生体系的建立对芸薹属蔬菜重要功能基因验证和重要遗传材料保存扩繁等具有重要意义。该文综述了影响芸薹属蔬菜(白菜类、甘蓝类和芥菜类)再生体系的主要因素:基因型、植物激素(NAA、BAP等)和外源添加物(硝酸银、抗坏血酸等),比较了不同影响因素对芸薹属蔬菜再生体系建立的影响差异。基因型方面表现为白菜类(AA基因组)较甘蓝类(CC基因组)和芥菜类(BB基因组)难再生。植物激素方面表现为适于芸薹属蔬菜预培养的激素一般为2,4-D和NAA+6-BA,分化培养一般为NAA+6-BA,且即使为同一类蔬菜,不同品种所需的激素浓度也不一致。外源添加物方面,硝酸银为白菜类蔬菜再生体系所必需,其也能促进甘蓝类和芥菜类的不定芽分化,但并不为两者所必需;低浓度的抗坏血酸可促进白菜类不定芽的分化和甘蓝类愈伤组织的形成。芸薹属蔬菜再生体系诱导的分子机理研究需进一步加强,同时不同芸薹属蔬菜再生条件差异产生的机理也需要深入研究。
The establishment of regeneration system is important for functional gene analysis, preservation and propagation of important genetic material in Brassica vegetables. This paper summarized the factors that affected regeneration system of Brassica vegetables, such as genotype, phytohormone, Ag NO3 and ascrobic acid, analyzed the differences of regeneration character under different factors among Brassica vegetables. In terms of genotype, Brassica rapa were more difficult regenerate than Brassica oleracea and Brassica juncea. For phytohormone, 2,4-D and NAA+6-BA were suitable for Brassica vegetables preculture and NAA+6-BA was fit for differentiation culture generally. Especially, different cultivar should be at different levels of hormones even at the same classification relationship. Ag NO3 was the necessary exogenous additive for Brassica rapa and also could promote adventitious bud differentiation of Brassica oleracea and Brassica juncea. Low concentration of ascorbic aicd could promote the adventitious buds differentiation of Brassica rapa and the callus formation of Brassica oleracea. For future research, the molecular mechanism of induced regeneration and the difference of regeneration system in different kinds of Brassica vegetables should be emphasized.
The establishment of regeneration system is important for functional gene analysis, preservation and propagation of important genetic material in Brassica vegetables. This paper summarized the factors that affected regeneration system of Brassica vegetables, such as genotype, phytohormone, Ag NO3 and ascrobic acid, analyzed the differences of regeneration character under different factors among Brassica vegetables. In terms of genotype, Brassica rapa were more difficult regenerate than Brassica oleracea and Brassica juncea. For phytohormone, 2,4-D and NAA+6-BA were suitable for Brassica vegetables preculture and NAA+6-BA was fit for differentiation culture generally. Especially, different cultivar should be at different levels of hormones even at the same classification relationship. Ag NO3 was the necessary exogenous additive for Brassica rapa and also could promote adventitious bud differentiation of Brassica oleracea and Brassica juncea. Low concentration of ascorbic aicd could promote the adventitious buds differentiation of Brassica rapa and the callus formation of Brassica oleracea. For future research, the molecular mechanism of induced regeneration and the difference of regeneration system in different kinds of Brassica vegetables should be emphasized.
引文
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