外源EBR对芥菜下胚轴伸长的影响
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摘要
油菜素内酯(BR)是一种植物激素,具有调控植物生长发育的作用。以芥菜为材料,采用外源2,4-表油菜素内酯(EBR)、乙烯利(Ethephon)、萘乙酸(NAA)等不同生长调节剂进行处理,初步研究了BR在芥菜下胚轴生长中的调控作用。研究发现,外源EBR处理在光照条件下可以促进芥菜下胚轴的伸长,而暗处理下却抑制下胚轴的生长;EBR与NAA协同促进下胚轴的伸长,EBR可以缓解Ethephon对下胚轴伸长的抑制作用,但EBR促进乙烯的合成。综上表明,光信号参与调控BR对下胚轴生长的调控作用,同时BR与乙烯、NAA等信号途径共同参与下胚轴的生长调控。
Brassinolide(BR) is a kind of plant hormones that has a function in regulating plant growth and development.In this paper, exogenous 2, 4-brassinolide(EBR), Ethephon and naphthylacetic acid(NAA) were used to explore the effects of brassinolide on the hypocotyl elongation of mustard seedlings. It was found that EBR could promote the mustard hypocotyl elongation under light conditions, while it had an inhibition effect under dark conditions. EBR and NAA had a synergistic promoting effect on the hypocotyl elongation. EBR could alleviate the inhibition effect of Ethephon on the hypocotyl elongation of mustard seedlings, but it could promoted the synthesis of ethylene. The results indicated that the light signal was involved in the regulation of hypocotyls growth by BR, at the same time, BR, ethylene and NAA signaling pathways were all involved in the regulation of the hypocotyl growth.
Brassinolide(BR) is a kind of plant hormones that has a function in regulating plant growth and development.In this paper, exogenous 2, 4-brassinolide(EBR), Ethephon and naphthylacetic acid(NAA) were used to explore the effects of brassinolide on the hypocotyl elongation of mustard seedlings. It was found that EBR could promote the mustard hypocotyl elongation under light conditions, while it had an inhibition effect under dark conditions. EBR and NAA had a synergistic promoting effect on the hypocotyl elongation. EBR could alleviate the inhibition effect of Ethephon on the hypocotyl elongation of mustard seedlings, but it could promoted the synthesis of ethylene. The results indicated that the light signal was involved in the regulation of hypocotyls growth by BR, at the same time, BR, ethylene and NAA signaling pathways were all involved in the regulation of the hypocotyl growth.
引文
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[13]ZHOU X Y,SONG L,XUE H W.Brassinosteroids regulate the differential growth of arabidopsis hypocotyls through auxin signaling components IAA19 and ARF7[J].Molecular Plant,2013,6(3):887-904.
[14]KIM B,JEONG Y J,CORVALAN C,et al.Darkness and gulliver2/phy B mutation decrease the abundance of phosphorylated BZR1 to activate brassinosteroid signaling in Arabidopsis[J].The Plant Journal,2014,77(5):737-747.
[15]LARSEN P B,CANCEL J D.Enhanced ethylene responsiveness in the Arabidopsis eer1 mutant results from a loss-of-function mutation in the protein phosphatase 2AA regulatory subunit,RCN1[J].The Plant Journal,2003,34(5):709-718.
[16]MCNELLIS T W,DENG XW.Light control of seedling morphogenetic pattern[J].Plant Cell,1995,7(11):1749-1761.
[17]王建华,徐同.油菜素内酯对酸雨伤害的棉花子叶膜脂质过氧化和乙烯产生的影响[J].湖北农学院学报,1991,11(4):1-6.
[18]HANSEN M,CHAE HS,KIEBER J J.Regulation of ACSprotein stability by cytokinin and brassinosteroid[J].Plant Journal for Cell&Molecular Biology,2009,57(4):606-614.
[19]GRAUWE L D E,VANDENBUSSCHE F,TIETZ O,et al.Auxin,ethylene and brassinosteroids:tripartite control of growth in the Arabidopsis thaliana hypocotyl[J].Plant&Cell Physiology,2005,46(6):827-836.
[20]DESLAURIERS S D,LARSEN P B.FERONIA Is a key modulator of brassinosteroid and ethylene responsiveness in Arabidopsis hypocotyls[J].Molecular Plant,2010,3(3):626-640.
[2]KHAN M,ROZHON W,BIGEARD J,et al.Brassinosteroid regulated GSK 3/Shaggy-like kinases phosphorylate mitogen-activated protein(MAP)kinase kinases,which control stomata development in Arabidopsis thaliana[J].Journal of Biological Chemistry,2013,288(11):7519-7527.
[3]HARTWIG T,CHUCK GS,FUJIOKA S,et al.Brassinosteroid contro1 of sex determination in maize[J].Proceedings of the National Academy of Sciences of the United States of America,2011,108(49):19814-19819.
[4]李雅洁,张其安,陆晓民.油菜素内酯对硝酸钙胁迫下黄瓜幼苗抗氧化系统及可溶性蛋白表达的影响[J].安徽科技学院学报,2017,31(1):35-40.
[5]王舒甜,张金池,张亮.油菜素内酯对盐胁迫下香樟叶片光合色素以及叶绿素荧光的影响[J].浙江大学学报(农业与生命科学版),2017,43(1):45-53.
[6]程彦伟,丁贺,韩建明,等.天然油菜素内酯对豆类种子发芽和胚根下胚轴伸长的影响[J].江苏农业科学,2014,42(9):140-142.
[7]刘灵,李海航,杨丽霞,等.油菜素内酯促进绿豆下胚轴不定根发生研究[J].科技导报,2007,5(25):227.
[8]徐如涓,郭一松,刘毓橘.表油菜素内酯对黄瓜下胚轴伸长,内源GA3,ABA及淀粉含量的影响[J].植物生理学报,1990,16(2):125-130.
[9]李元元,曹清河.油菜素内酯参与调控植物生长发育与抗逆性的机制及其育种应用研究[J].中国农业科技导报,2015,17(2):25-32.
[10]李凯荣,韩刚.天然油菜素内酯促进柠条和紫穗槐种子发芽及下胚轴伸长效应[J].西北林学院学报,2003,18(4):17-20.
[11]程彦伟,丁贺,韩建明,等.天然油菜素内酯对豆类种子发芽和胚根下胚轴伸长的影响[J].江苏农业科学,2014,42(9):140-142.
[12]GUDESBLAT G E,RUSSINOVA E.Plants grow on brassinosteroids[J].Current Opinion in Plant Biology,2011,14(5):530.
[13]ZHOU X Y,SONG L,XUE H W.Brassinosteroids regulate the differential growth of arabidopsis hypocotyls through auxin signaling components IAA19 and ARF7[J].Molecular Plant,2013,6(3):887-904.
[14]KIM B,JEONG Y J,CORVALAN C,et al.Darkness and gulliver2/phy B mutation decrease the abundance of phosphorylated BZR1 to activate brassinosteroid signaling in Arabidopsis[J].The Plant Journal,2014,77(5):737-747.
[15]LARSEN P B,CANCEL J D.Enhanced ethylene responsiveness in the Arabidopsis eer1 mutant results from a loss-of-function mutation in the protein phosphatase 2AA regulatory subunit,RCN1[J].The Plant Journal,2003,34(5):709-718.
[16]MCNELLIS T W,DENG XW.Light control of seedling morphogenetic pattern[J].Plant Cell,1995,7(11):1749-1761.
[17]王建华,徐同.油菜素内酯对酸雨伤害的棉花子叶膜脂质过氧化和乙烯产生的影响[J].湖北农学院学报,1991,11(4):1-6.
[18]HANSEN M,CHAE HS,KIEBER J J.Regulation of ACSprotein stability by cytokinin and brassinosteroid[J].Plant Journal for Cell&Molecular Biology,2009,57(4):606-614.
[19]GRAUWE L D E,VANDENBUSSCHE F,TIETZ O,et al.Auxin,ethylene and brassinosteroids:tripartite control of growth in the Arabidopsis thaliana hypocotyl[J].Plant&Cell Physiology,2005,46(6):827-836.
[20]DESLAURIERS S D,LARSEN P B.FERONIA Is a key modulator of brassinosteroid and ethylene responsiveness in Arabidopsis hypocotyls[J].Molecular Plant,2010,3(3):626-640.