芥菜遗传多样性SRAP分析
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摘要
以44个芥菜品种为材料,利用SRAP分子标记对其进行遗传多样性研究。从100个不同的引物组合中筛选出了31对多态性较高,条带清晰且稳定的引物,对44个芥菜材料DNA进行PCR扩增。结果表明,这31对引物共产生了286条条带,其中多态性条带173条,多态率达60.49%。平均每对引物扩增条带9.23条。用NTSYS统计软件进行聚类分析,44个芥菜品种的遗传相似系数为0.61~0.89,平均系数为0.75。在相似系数为0.67的水平,44个芥菜品种可分为4大类,与形态学分类结果基本一致。
An SRAP marker was applied to analyze the genetic diversity of 44 accessions of Brassica juncea(L.).Thirty-one pairs of SRAP primer combinations,which amplified stable bands with clear and higher polymorphism,were selected by apreliminary screening.A total of 286 DNA bands were amplified from the 44 accessions of B.junceaby PCR amplification.Among them,60.49%or 173 DNA bands were polymorphic.The average number of DNA bands amplified by each pair of primer combination was 9.23.The clustering analysis by NTSYS statistical software showed that the genetic similarity coefficients were in the range of 0.61-0.89 averaging 0.75.At the coefficient of 0.67,the 44 accessions were divided into 4 groups which were identical with the result obtained by the morphological classification.
An SRAP marker was applied to analyze the genetic diversity of 44 accessions of Brassica juncea(L.).Thirty-one pairs of SRAP primer combinations,which amplified stable bands with clear and higher polymorphism,were selected by apreliminary screening.A total of 286 DNA bands were amplified from the 44 accessions of B.junceaby PCR amplification.Among them,60.49%or 173 DNA bands were polymorphic.The average number of DNA bands amplified by each pair of primer combination was 9.23.The clustering analysis by NTSYS statistical software showed that the genetic similarity coefficients were in the range of 0.61-0.89 averaging 0.75.At the coefficient of 0.67,the 44 accessions were divided into 4 groups which were identical with the result obtained by the morphological classification.
引文
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[10]乔爱民,刘佩瑛,雷建军,芥菜16个变种的RAPD研究[J].植物学报,1998,40(10):915-921.
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[12]刘佩瑛.中国芥菜[M].北京:中国农业出版社,1995:56-59.
[13]付杰.中国芥菜遗传多样性与亲缘关系的研究[D].杭州:浙江大学,2005.
[14]齐晓花.中国芥菜系统进化研究及重要数量性状的遗传分析[D].杭州:浙江大学,2009.
[15]宋明,刘婷,汤青林,等.芥菜种质资源的RAPD和ISSR分析.园艺学报,2009,36(6):835-842.
[2]范永红,沈进娟,董代文.芥菜类蔬菜产业发展现状及研究前景思考[J].农学学报,2016,6(2):65-71.
[3]刘佩瑛.中国芥菜[M].北京:中国农业出版社,1996.
[4]刘淑艳,刘忠松,官春云.芥菜型油菜种质资源研究进展[J].植物遗传资源学报,2007,8(3):351-358.
[5]刘忠松,官春云,李木旬,等.甘蓝型油菜与芥菜型油菜种间杂交研究[J].中国油料作物学报,2001,23(2):82-87.
[6]顾祥昆,李菲,张淑江,等.芥菜游离小孢子培养技术研究[J].中国蔬菜,2013(12):23-30.
[7]蒋先军,骆永明,赵其国.重金属污染土壤的植物修复研究Ⅲ.金属富集植物Brassica juncea对锌镉的吸收和积累[J].土壤学报,2002,39(5)71-74.
[8]张雁,黄丽慧,陈于陇,等.不同品种芥菜发酵过程中亚硝酸盐变化规律的研究[J].现代食品科技,2013,29(9):2152-2157.
[9]孙涛,张玉秀,柴团耀.印度芥菜(Brassica juncea L.)重金属耐性机理研究进展[J].中国生态农业学报,2011,19(1):226-234.
[10]乔爱民,刘佩瑛,雷建军,芥菜16个变种的RAPD研究[J].植物学报,1998,40(10):915-921.
[11]李宁,司军,任雪松,等.芥菜种质资源遗传多样性和亲缘关系的SRAP分析[J].中国蔬菜,2014(5):26-33.
[12]刘佩瑛.中国芥菜[M].北京:中国农业出版社,1995:56-59.
[13]付杰.中国芥菜遗传多样性与亲缘关系的研究[D].杭州:浙江大学,2005.
[14]齐晓花.中国芥菜系统进化研究及重要数量性状的遗传分析[D].杭州:浙江大学,2009.
[15]宋明,刘婷,汤青林,等.芥菜种质资源的RAPD和ISSR分析.园艺学报,2009,36(6):835-842.