固相萃取-反相高效液相色谱法对泼尼松龙片剂有效含量的测定

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英文篇名：Determination of Prednisolone Tablets by Solid Phase Extraction and Reversed Phase High Performance Liquid Chromatography 作者：张慧 英文作者：Zhang hui;zhejiang xianju pharmaceutical Co.,Ltd; 关键词：泼尼松龙 ; 固相萃取 ; 液相色谱 英文关键词：prednisolone;;solid phase extraction (SPE);;high-performance liquid chromatography(HPLC) 中文刊名：YNHG 英文刊名：Yunnan Chemical Technology 机构：浙江仙琚制药股份有限公司; 出版日期：2019-05-17 17:40 出版单位：云南化工 年：2019 期：v.46;No.238 语种：中文; 页：YNHG201903029 页数：2 CN：03 ISSN：53-1087/TQ 分类号：85-86

摘要

采用固相萃取-反相高效液相色谱法测定泼尼松龙含量。以甲醇-乙酸缓冲液作为提取液,经Oasis HLB固相萃取柱净化,氮吹仪浓缩定容,经液相C18色谱柱分离,在247nm波长下检测。结果表明泼尼松龙线性相关系数为0.9992,变异系数为1.23%,回收率为87.4%～93.7%。
        A method was developed for the deter mination of prednisolone by solid phase extraction and reversed phase high performance liquid chromatography(SPE-RPHPLC).The samples were extracted with a methol-acetate buffer solution,purified by an Oasis HLB SPE column,concentrated and analysised by HPLC on C18 column.The correlation coefficients were of 0.9992 with relative standard deviations of1.23%,and the recoveries of prednisolone were in the range of 87.4%～93.7%.

引文

[1]付春梅,刘三康,李章万.反相高效液相色谱法测定泼尼松龙磷酸钠原料药及其注射液色谱2006,24(1).
    [2]黄延盛,栾心怡,王艺霏,等. LC-MS/MS法测定Beagle犬血浆中泼尼松的浓度药物分析杂志2007,5.
    [3]SN/T 2160-2008动物源食品中氢化泼尼松残留量检测方法气相色谱-质谱/质谱法.
    [4]GB/T 22986-2008牛奶和奶粉中氢化泼尼松残留量的测定液相色谱-串联质谱法.
    [5]国家药典委员会,中国药典二部,2015,707.