UHPLC法测定八宝惊风散中沉香四醇的含量
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摘要
目的建立超高效液相色谱(UHPLC)法测定八宝惊风散中沉香四醇含量的方法。方法采用Agilent Poroshell 120 SBC18(100 mm×4.6 mm,2.7μm)为色谱柱固定相,以乙腈(A)-0.1%甲酸水溶液(B)为流动相,梯度洗脱,流速为0.8 mL·min~(-1);柱温为40℃;检测波长为252 nm。结果沉香四醇实现较好地分离,在4.02~40.2μg·mL~(-1)范围内线性良好(r=0.999 9);平均回收率为98.90%(n=6),RSD为1.64%。结论该方法测定结果准确可靠,重现性好,可以作为八宝惊风散质量控制的参考依据。
OBJECTIVE To establish a method for the determination of Agarotetrol in Babaojingfeng powder. METHODS The Agarotetrol was performed on a Agilent Poroshell 120 SB-C18( 100 mm × 4. 6 mm,2. 7 μm) column using a mobile phase of acetonitrile-0.1% formic acid in water; and the detective wavelength was 252 nm. RESULTS The calibration curve of agarotetrol were linear with the concentration range of 4.02-40.2 μg·mL~(-1)( r = 0.999 9).The accuracy was good with average recovery was 98.90%with RSD 1.64%,respectively. CONCLUSION This method is accurate,reliable and reproducible,it can be used for the quality control of Babao jingfeng powder.
OBJECTIVE To establish a method for the determination of Agarotetrol in Babaojingfeng powder. METHODS The Agarotetrol was performed on a Agilent Poroshell 120 SB-C18( 100 mm × 4. 6 mm,2. 7 μm) column using a mobile phase of acetonitrile-0.1% formic acid in water; and the detective wavelength was 252 nm. RESULTS The calibration curve of agarotetrol were linear with the concentration range of 4.02-40.2 μg·mL~(-1)( r = 0.999 9).The accuracy was good with average recovery was 98.90%with RSD 1.64%,respectively. CONCLUSION This method is accurate,reliable and reproducible,it can be used for the quality control of Babao jingfeng powder.
引文
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[11]顾宇凡,张倩,霍会霞,等.HPLC-DAD测定沉香药材中沉香四醇的含量[J].世界科学技术-中医药现代化,2014,16(12):2643-2646.
[12]李远彬,王羚郦,邓幸运,等.沉香的醇浸出物和沉香四醇含量调查及品质分类初探[J].中国实验方剂学杂志,2017,23(13):67-72.
[2]陈繁华,曾玉梅.八宝惊风散中天麻素的鉴别和含量测定[J].解放军药学学报,2013,29(1):63-65.
[3]隆颖,栗建明,孙丽丽.八宝惊风散质量标准研究[J].中国当代医药,2015,22(12):17-21.
[4]唐丽丽,何绍萍,陈华龙.八宝惊风散的定性定量方法[J].中国实验方剂学杂志,2013,19(19):146-148.
[5]杨宝,刘佳,陈繁华.气相色谱法测定八宝惊风散中的薄荷脑、龙脑、丁香酚和麝香酮的含量[J].中南药学,2016,14(10):1113-1116.
[6]陈繁华,曾玉梅,姚海燕.原子吸收光谱法测定八宝惊风散中重金属元素[J].中成药,2013,35(9):1937-1939.
[7]林恬聪,赵东海,蔡秋林.原子荧光光谱法测定八宝惊风散中的砷含量[J].海峡药学,2012,24(12):80-81.
[8]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:185.
[9]马龙婧.中药市场上名贵药材的现状探讨[J].北方药学,2015,12(4):133.
[10]梅全喜,林焕泽,李红念.沉香的药用历史、品种、产地研究应用浅述[J].中国中医药现代远程教育,2013,11(8):85-88.
[11]顾宇凡,张倩,霍会霞,等.HPLC-DAD测定沉香药材中沉香四醇的含量[J].世界科学技术-中医药现代化,2014,16(12):2643-2646.
[12]李远彬,王羚郦,邓幸运,等.沉香的醇浸出物和沉香四醇含量调查及品质分类初探[J].中国实验方剂学杂志,2017,23(13):67-72.