HPLC法测定清心沉香八味散中沉香四醇的含量
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摘要
目的:测定清心沉香八味散中沉香四醇的含量。方法:采用高效液相色谱法,以十八烷基硅烷键合硅胶为填充剂,以乙腈为流动相A,0.1%甲酸溶液为流动相B,柱温为30℃;流速1.0m L/min;检测波长为252nm。结果:沉香四醇进样量在0.004985~0.074775μg范围之内与两个峰面积之和具有良好的线性关系(r=0.9999);平均加样回收率为98.3%,RSD值0.9%(n=6)。结论:该方法稳定、准确,可用于清心沉香八味散的质量控制。
Obiective The content of agarotetrol in Qingxinchenxiangbawei Powder was determined. Methods The HPLC analysis was performed on a column packed with octadecylsilane bonded silica gel as filler in the temperature of 30℃,eluted with acetonitrile as mobile phase A and 0. 1% formic acid solution as the mobile phase B,with the flow rate of 1. 0 ml/min. The detection wavelength was set at 252 nm. Results There was a good linear relationship between 0. 004985 and 0. 074775μg in the injection volume of agarotetrol( r = 0. 99999),with the average recoveries were 98. 3% and the RSD was 0. 9%( n = 6). Conclusion The method is stable and accurate,and can be used for quality control of Qingxinchenxiangbawei Powder.
Obiective The content of agarotetrol in Qingxinchenxiangbawei Powder was determined. Methods The HPLC analysis was performed on a column packed with octadecylsilane bonded silica gel as filler in the temperature of 30℃,eluted with acetonitrile as mobile phase A and 0. 1% formic acid solution as the mobile phase B,with the flow rate of 1. 0 ml/min. The detection wavelength was set at 252 nm. Results There was a good linear relationship between 0. 004985 and 0. 074775μg in the injection volume of agarotetrol( r = 0. 99999),with the average recoveries were 98. 3% and the RSD was 0. 9%( n = 6). Conclusion The method is stable and accurate,and can be used for quality control of Qingxinchenxiangbawei Powder.
引文
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[2]李红念,梅全喜,林焕泽,等.沉香的化学成分、药理作用和临床应用研究进展[J].中国药房,2011,22(35):3349-3351.
[3]顾宇凡,张倩,霍会霞,等.HPLC-DAD测定沉香药材中沉香四醇的含量[J].世界科学技术-中医药现代化,2014(12):2643-2646.
[4]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2015.
[5]南京中医药大学编.中药大辞典[M].上海:上海科技出版社,2006.
[6]常新全,丁丽霞.中药活性成分分析手册[M].北京:学苑出版社,2002.
[7]吴泽青,熊礼燕,帅欧,等.3种不同方法形成的沉香药材色酮含量测定[J].中药新药与临床药理,2015(3):356-360.
[8]郅景梅.沉香药材中两种活性成分的含量测定研究[J].健康必读(下旬刊),2013(1):301-302.