响应面法优化鹰嘴豆发酵纳豆工艺
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摘要
鹰嘴豆作为纳豆发酵原料,以纳豆激酶(Nattokinase,NK)活性为指标,在单因素实验的基础上,利用Box-Behnken实验设计以及响应面分析优化纳豆发酵工艺。研究结果表明,鹰嘴豆的最佳发酵工艺条件为:基质含水量50%,接种量6%,发酵温度34℃,发酵时间48h,后熟时间20h;对优化的工艺条件进行验证,纳豆激酶活性为986.324IU/g,与预测值接近。
Using nattokinase activity as an index,the fermentation process of natto is optimized by Box-Behnken experimental design and response surface analysis with chickpea as the raw material based on the single factor test.The results show that the optimum fermentation conditions of chickpea are as follows:the substrate water content is 50%,the inoculation amount is 6%,the fermentation temperature is 34 ℃,the fermentation time is 48 hand the ripening time is 20 h.The optimized process conditions are verified and the NK activity is 986.324 IU/g,which is close to the predicted value.
Using nattokinase activity as an index,the fermentation process of natto is optimized by Box-Behnken experimental design and response surface analysis with chickpea as the raw material based on the single factor test.The results show that the optimum fermentation conditions of chickpea are as follows:the substrate water content is 50%,the inoculation amount is 6%,the fermentation temperature is 34 ℃,the fermentation time is 48 hand the ripening time is 20 h.The optimized process conditions are verified and the NK activity is 986.324 IU/g,which is close to the predicted value.
引文
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[2]聂石辉,彭琳,王仙,等.鹰嘴豆种质资源农艺性状遗传多样性分析[J].植物遗传资源学报,2015,16(1):64-70.
[3]张涛,江波,沐万孟.酶法改性对鹰嘴豆分离蛋白功能性的影响[J].食品与发酵工业,2007,33(4):56-60.
[4]魏玲,李学琴,王莉,等.反胶束体系萃取鹰嘴豆蛋白的工艺研究[J].中国粮油学报,2007,22(3):137-139.
[5]汪建红,张婷,张蔚佼,等.鹰嘴豆多糖抗疲劳生物功效及其机制研究[J].食品工业,2009(5):1-3.
[6]张涛,江波,沐万孟.鹰嘴豆分离蛋白分离纯化[J].食品科学,2008,29(3):158-161.
[7]高瑞萍,刘辉,刘嘉,等.纳豆的研究进展[J].食品与发酵科技,2011,47(1):23-26.
[8]杨亚平.纳豆成分的保健功效[J].大豆科技,2015(6):26-28.
[9]朱诗萌.纳豆的干燥工艺及其微胶囊化的研究[D].吉林:吉林大学,2016.
[10]Sumi H,Hamada H,Tsushima H,et al.A novel fibrinolytic enzyme(nattokinase)in the vegetable cheese natto;a typical and popular soybean food in the Japanese diet[J].Experientia,1987,43(10):1110-1111.
[11]Abdui G,Sri R L.Effect of black soybean natto extract(Glycine soja)on reproduction system of hypercholesterolemia male mice[J].Asian Pac J Reprod,2016(5):387-390.
[12]Hsia C H,Shen M C,Lin J S,et al.Nattokinase decreases plasma levels of fibrinogen,factor VII,and factor VIII in human subjects[J].Nutr Res,2009,29(3):190-196.
[13]齐香君,桂丽.纳豆激酶活性测定的方法学研究[J].中国新药杂志,2007,16(10):769-772.