银杏叶多酚物质不同提取方法的比较研究
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摘要
【目的】观察不同提取方法对银杏叶中多酚成分含量的影响,为其进一步开发提供依据。【方法】采用乙醇提取法、酶解—乙醇提取法、超声波—乙醇提取法、微波—乙醇提取法制备银杏叶多酚成分,以称质量法测定提取物得率,福林—酚比色法测定多酚含量。【结果】应用不同提取方法的银杏叶多酚提取物得率及含量分别为:乙醇提取法(11.78±0.13)%、(5.42±0.06)%,酶解—乙醇提取法(12.71±0.09)%、(6.89±1.04)%,超声波—乙醇提取法(12.45±0.11)%、(6.71±0.06)%,微波—乙醇提取法(11.39±0.10)%、(5.97±1.00)%。经统计学分析,与乙醇提取法相比,酶解—乙醇提取法和超声波—乙醇提取法多酚提取物含量更高(P <0.05或P <0.01)。【结论】以上4种提取方法所得银杏叶多酚提取物的得率和含量不一,酶解—乙醇提取法和超声波—乙醇提取法更优。
Objective To observe the effects of different extraction methods on the contents of polyphenols from Folium Ginkgo, thus to provide evidence for further development. Methods The ethanol extraction method,cellulose enzyme-assisted extraction technology, ultrasonic-assisted extraction technology, and microwaveassisted extraction technology were adopted to prepare the polyphenols from Folium Ginkgo. The yield of polyphenos was determined by weighing method,and the content of polyphenols was measured by Folin-Ciocalteu colorimetry. Results The yields and contents of polyphenols extracted from Folium Ginkgo by different extraction methods were as follows respectively:the ethanol extraction method,(11.78 ± 0.13)%,(5.42 ± 0.06)%;the cellulose enzyme-assisted extraction technology,(12.71 ± 0.09)%,(6.89 ± 1.04)%;the ultrasonic-assisted extraction technology,(12.45 ± 0.11)%,(6.71 ± 0.06)%; the microwave-assisted extraction technology,(11.39 ± 0.10)%,(5.97±1.00)%. The results of statistical analysis showed that compared with the ethanol extraction method,the content of polyphenols extracted by the cellulose enzyme-assisted extraction technology and the ultrasonic-assisted extraction technology were higher(P < 0.05 or P < 0.01). Conclusion The yields and contents of polyphenols extracted from Folium Ginkgo by the above 4 extraction methods are different,and the cellulose enzyme-assisted extraction technology and the ultrasonic-assisted extraction technology can achieve higher yields and contents.
Objective To observe the effects of different extraction methods on the contents of polyphenols from Folium Ginkgo, thus to provide evidence for further development. Methods The ethanol extraction method,cellulose enzyme-assisted extraction technology, ultrasonic-assisted extraction technology, and microwaveassisted extraction technology were adopted to prepare the polyphenols from Folium Ginkgo. The yield of polyphenos was determined by weighing method,and the content of polyphenols was measured by Folin-Ciocalteu colorimetry. Results The yields and contents of polyphenols extracted from Folium Ginkgo by different extraction methods were as follows respectively:the ethanol extraction method,(11.78 ± 0.13)%,(5.42 ± 0.06)%;the cellulose enzyme-assisted extraction technology,(12.71 ± 0.09)%,(6.89 ± 1.04)%;the ultrasonic-assisted extraction technology,(12.45 ± 0.11)%,(6.71 ± 0.06)%; the microwave-assisted extraction technology,(11.39 ± 0.10)%,(5.97±1.00)%. The results of statistical analysis showed that compared with the ethanol extraction method,the content of polyphenols extracted by the cellulose enzyme-assisted extraction technology and the ultrasonic-assisted extraction technology were higher(P < 0.05 or P < 0.01). Conclusion The yields and contents of polyphenols extracted from Folium Ginkgo by the above 4 extraction methods are different,and the cellulose enzyme-assisted extraction technology and the ultrasonic-assisted extraction technology can achieve higher yields and contents.
引文
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[2]Duchnowicz P,Bors M,Podsedek A,et al.Effect of polyphenols extracts from brassica vegetables on erythrocyte membranes(in vitro study)[J].Environ Toxicol Phar,2012,34(3):783.
[3]Qin X Y,Cheng Y,Yu L C,et al.Potential protection of green tea polyphenols against ntracellular amyloid beta-induced toxicity on primary cultured prefrontal cortical neurons of rats[J].Neurosci Lett,2012,513(2):170.
[4]曹春艳.响应面法优化银杏叶黄酮提取工艺[J].中国食品学报,2014,14(4):78.
[5]涂宝军,李勇,董玉玮,等.银杏外种皮多酚提取工艺及抗氧化性能研究[J].食品工业科技,2014,35(20):340.
[6]吴梅林,周春山,陈龙胜,等.酶法提取银杏黄酮类化合物研究[J].天然产物研究与开发,2004,16(6):557.
[7]王蕙,付燕,郎久义,等.酶法及半仿生法提取银杏黄酮的工艺研究[J].辽宁中医药大学学报,2009,11(2):146.
[8]王桃云,邱业先,汪金莲,等.响应面法优化银杏叶多酚提取工艺及对脲酶抑制活性的研究[J].化学研究与应用,2013,25(7):999.
[9]海洪,王新雯,金文英,等.响应面法优化超声波提取银杏叶黄酮的工艺研究[J].辽宁中医杂志,2010,37(8):1559.
[10]曾里,刘世贵.微波技术应用于银杏黄酮浸提的研究[J].四川大学学报(自然科学版),2004,41(4):833.
[11]黄莉莉,汪良峰,崔政伟.介尺度下微波和超声波辅助提取银杏黄酮的比较研究[J].食品科技,2017,42(5):188.
[12]张科卫,马彩霞,缪六舒.Folin-Ciocaileu比色法测定不同产地生姜中总酚[J].中成药,2012,34(12):2450.
[13]孔登,武希江,李万忠,等.不同产地、生长年限银杏叶总多酚含量比较[J].中成药,2017,39(6):1236.
[14]王赛君,伍振锋,杨明,等.中药提取新技术及其在国内的转化应用研究[J].中国中药杂志,2014,39(8):1360.