沙参麦冬汤对胃癌小鼠K-ras基因表达的影响
摘要
目的:观察RT-PCR检测沙参麦冬汤对胃癌小鼠模型癌基因K-ras的影响。方法:建立MFC胃癌小鼠模型,予沙参麦冬汤灌胃治疗4周后,采用荧光定量RT-PCR检测K-ras基因的表达水平。结果:干预组经沙参麦冬汤处理4周后,K-ras mRNA的表达显著低于未用沙参麦冬汤处理的模型组(t=2.724.,P=0.018)。结论:沙参麦冬汤可以下调MFC胃癌小鼠K-ras基因的表达,其机制可能为抑制瘤体组织中K-ras基因的过表达,干预K-ras的突变或稳定Kras基因的表达来调控癌变的进展。
引文
[1]Jemal A,Siegel R,Ward E,et al.Cancer statistics,2008[J].CA Cancer J Clin,2008,58(2):71-96.
[2]金懋林.胃癌药物治疗研究进展[J].临床药物治疗杂志,2006,4(2):13-15.
[3]孙大志,许玲,姜林娣,等.中医药治疗胃癌临床研究文献质量的调查评价[J].中国临床医学,2003,10:833-835.
[4]Yao J C,Shimada K,Ajani JA.Adjuvant therapy for gastric carcinoma:closing out the century[J].Oncology(Huntingt)1999,13:1485-1494.
[5]朱静,陈小飞.食管癌分子靶向治疗[J].东南大学学报:医学版,2011,39(5):623-626.
[6]张瑞定.沙参麦冬汤加减治疗慢性胃炎30例临床观察[J].内蒙古中医药,2012,10:14.
[7]黄兴彬.参麦冬汤合平胃散化裁治疗萎缩性胃炎40例[J].中国民族民间医药,2010,6:153.
[8]中广志.沙参麦冬汤加味治疗放射性肺炎的临床观察[J].实用中医内科杂志,1996,10(4):37-381.
[9]蓝祚均.沙参麦冬汤减轻鼻咽癌放疗损伤的临床观察[J].福建中医药,1997,28(1):141.
[10]徐叔云,卞如濂,陈修.药理实验方法学[M].北京:人民卫生出版社,2002.1121-1124,1757-1784.
[11]王克穷,张振兴,张天瑶,等.四君子汤对胃癌细胞植入小鼠术后复发细胞内VEGF及生存期的影响[J].福建中医药,2012,43(1):43-44.
[12]Maehara Y,Kabashima A,Koga T,et al.Vascular invasion and potentialfor tumor angiogenesis and metastasizing gastric carcinoma[J].Surgery,2000,128(3):408.
[13]Giulia A,Erlet S,Mike M,et al.FLAG assay as a novel method for real-time signal generation during PCR:application to detection and genotyping of k-ras colon 12 mutations[J].Nucleic Acids Res,2007,35(19):131-139.
[14]Vatan O,Bilaloglu R,Tunca B,et al.Low frequency of p53 and k-ras colon 12 mutations in non-small cell lung carcinoma(NSCLC)tumors and surgical margins[J].Tumor Res,2007,93(5):473-477.
[15]Schubbert S,Bollag G,Lyubynska N,et al.Biochemical and functional characterization of germ line KRAS mutations[J].Mol Cell Biol,2007,27(22):7765-7770.
[16]Margaret P,Steven E,Mark R,et al.Activated Kras,but not Hras or Nras,may initiate tumors of endodermal originvia stem cell expansion[J].Mol Cell Biol,2008,28(8):2659-2674.
[17]Fotiadou P,Takahashi C,Rajabi H,et al.Wild-type NRas and KRas perform distinct func-tions during transformation[J].Mol Cell Biol.2007,27(19):6742-6755.
[18]辛晓明,张倩,王浩,等.南沙参的化学成分及药效学研究进展[J].中国实用医药,2008,28(3):188-189.
[19]许秋菊,侯莉莉,胡国强,等.麦冬皂苷B诱导人宫颈癌Hela细胞自噬的机制[J].药学学报,2013,48(6):855-859.
[2]金懋林.胃癌药物治疗研究进展[J].临床药物治疗杂志,2006,4(2):13-15.
[3]孙大志,许玲,姜林娣,等.中医药治疗胃癌临床研究文献质量的调查评价[J].中国临床医学,2003,10:833-835.
[4]Yao J C,Shimada K,Ajani JA.Adjuvant therapy for gastric carcinoma:closing out the century[J].Oncology(Huntingt)1999,13:1485-1494.
[5]朱静,陈小飞.食管癌分子靶向治疗[J].东南大学学报:医学版,2011,39(5):623-626.
[6]张瑞定.沙参麦冬汤加减治疗慢性胃炎30例临床观察[J].内蒙古中医药,2012,10:14.
[7]黄兴彬.参麦冬汤合平胃散化裁治疗萎缩性胃炎40例[J].中国民族民间医药,2010,6:153.
[8]中广志.沙参麦冬汤加味治疗放射性肺炎的临床观察[J].实用中医内科杂志,1996,10(4):37-381.
[9]蓝祚均.沙参麦冬汤减轻鼻咽癌放疗损伤的临床观察[J].福建中医药,1997,28(1):141.
[10]徐叔云,卞如濂,陈修.药理实验方法学[M].北京:人民卫生出版社,2002.1121-1124,1757-1784.
[11]王克穷,张振兴,张天瑶,等.四君子汤对胃癌细胞植入小鼠术后复发细胞内VEGF及生存期的影响[J].福建中医药,2012,43(1):43-44.
[12]Maehara Y,Kabashima A,Koga T,et al.Vascular invasion and potentialfor tumor angiogenesis and metastasizing gastric carcinoma[J].Surgery,2000,128(3):408.
[13]Giulia A,Erlet S,Mike M,et al.FLAG assay as a novel method for real-time signal generation during PCR:application to detection and genotyping of k-ras colon 12 mutations[J].Nucleic Acids Res,2007,35(19):131-139.
[14]Vatan O,Bilaloglu R,Tunca B,et al.Low frequency of p53 and k-ras colon 12 mutations in non-small cell lung carcinoma(NSCLC)tumors and surgical margins[J].Tumor Res,2007,93(5):473-477.
[15]Schubbert S,Bollag G,Lyubynska N,et al.Biochemical and functional characterization of germ line KRAS mutations[J].Mol Cell Biol,2007,27(22):7765-7770.
[16]Margaret P,Steven E,Mark R,et al.Activated Kras,but not Hras or Nras,may initiate tumors of endodermal originvia stem cell expansion[J].Mol Cell Biol,2008,28(8):2659-2674.
[17]Fotiadou P,Takahashi C,Rajabi H,et al.Wild-type NRas and KRas perform distinct func-tions during transformation[J].Mol Cell Biol.2007,27(19):6742-6755.
[18]辛晓明,张倩,王浩,等.南沙参的化学成分及药效学研究进展[J].中国实用医药,2008,28(3):188-189.
[19]许秋菊,侯莉莉,胡国强,等.麦冬皂苷B诱导人宫颈癌Hela细胞自噬的机制[J].药学学报,2013,48(6):855-859.