温度、光照及PEG胁迫对不同生态型醉马草种子萌发的影响
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摘要
为研究醉马草(Achnatherum inebrians)种群繁殖和环境适应机理,本研究从甘肃榆中(YZ)和内蒙古阿拉善(Alxa)采集的两个醉马草种群的种子作为试验材料,研究不同温度(10℃/20℃、15℃/25℃和20℃/30℃变温12 h/12 h)、光照(完全光照和完全黑暗)以及PEG浓度(0、–0.3、–0.6和–0.9 MPa)共同作用对两种醉马草种子发芽率、发芽指数、胚根和胚芽长的影响。结果表明,在一定的渗透胁迫范围内(–0.6和–0.9 Mpa),温度对醉马草种子萌发有显著影响,15℃/25℃条件下,各水分处理的两种醉马草的发芽率、发芽指数、胚根长和胚芽长均达到最大值,该温度为醉马草的最适萌发温度;光照对两种醉马草种子的发芽率和发芽指数没有显著影响;在同一温度条件下,两种醉马草的发芽率、发芽指数、胚芽长均随着PEG浓度的升高而减小,且均与无菌水对照差异显著(P <0.05);与YZ醉马草种子相比,Alxa醉马草种子萌发的速度和整齐度较好,种子的活力较强。因此,无论是在光照、温度或水分胁迫条件下,Alxa醉马草与YZ醉马草相比更具有较强的抗逆性和适应性,可作为醉马草繁殖扩散和开发利用的优良草种。
The percent seed germination, germination index, radical length, and plumule length of Achnatherum inebrians seeds and seedlings of the Yuzhong and Alax ecotypes were measured in treatments with different temperatures(10 ℃/20 ℃、15 ℃/25 ℃ 和 20 ℃/30 ℃, all under a 12/12 h light/dark cycle), light conditions(24 h light and 24 h dark), and PEG(polyethylene glycol) concentrations(0, –0.3, –0.6, and –0.9 MPa). The results showed that 15 ℃/25 ℃ was the most suitable temperature for the seed germination of both Achnatherum inebrians ecotypes. The generation rate and index, radical length, and plumule length of these two ecotypes significantly decreased under treatments with increasing PEG stress levels.Under the combined action of all three factors, the radical length and plumule length of the Alax ecotype of Achnatherum inebrians were greater than those of the Yuzhong ecotype.
The percent seed germination, germination index, radical length, and plumule length of Achnatherum inebrians seeds and seedlings of the Yuzhong and Alax ecotypes were measured in treatments with different temperatures(10 ℃/20 ℃、15 ℃/25 ℃ 和 20 ℃/30 ℃, all under a 12/12 h light/dark cycle), light conditions(24 h light and 24 h dark), and PEG(polyethylene glycol) concentrations(0, –0.3, –0.6, and –0.9 MPa). The results showed that 15 ℃/25 ℃ was the most suitable temperature for the seed germination of both Achnatherum inebrians ecotypes. The generation rate and index, radical length, and plumule length of these two ecotypes significantly decreased under treatments with increasing PEG stress levels.Under the combined action of all three factors, the radical length and plumule length of the Alax ecotype of Achnatherum inebrians were greater than those of the Yuzhong ecotype.
引文
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[13]万志文,王萍,张兴旭,李春杰.锰、水杨酸与内生真菌互作对醉马草种子萌发的影响.草地学报,2016,24(1):107-113.WAN Z W,WANG P,ZHANG X X,LI C J.Effects of manganese and SA on germination of Achnatherum inebrians seed containing or free of endophyte.Acta Agrestia Sinica,2016,24(1):107-113.
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