沙棘果渣总黄酮的降血脂及降血糖作用
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摘要
目的:探讨沙棘果渣总黄酮的降血脂及降血糖作用。方法:昆明种雄性小鼠72只适应环境7 d后,随机分成6组(n=12):空白对照组、模型组、辛伐他丁组,总黄酮小、中、高剂量组。除空白对照组外,其余各组喂高脂饲料诱发高血脂症,每天称小鼠体质量1次,按体质量更换给药剂量。试验第40天取血后解剖小鼠,检测TC、TG、HDL-C、LDL-C含量,观测体质量及脏器指数的变化情况。采用小鼠高血糖模型降糖实验方法考察沙棘果渣总黄酮的降血糖活性。结果:沙棘果渣总黄酮各剂量与模型组比较,可以显著降低TC、TG和LDL-C的含量(P<0.05),显著升高HDL-C的含量(P<0.05);沙棘果渣总黄酮组空腹血糖值显著降低(P<0.05)。结论:沙棘果渣总黄酮具有降血脂及降血糖活性。
Objective: To study the hypolipidemic and hypoglycemic effects of total flavonoids from pomace of Fructus Hippophae.Methods: A total of 72 Kunming male mice were randomly divided into six groups( n = 12 in each group) : normal group,model group,simvastatin group,low-dose flavonoid group,middle-dose flavonoid group and high-dose flavonoid group. Except the blank control group,the mice were fed with high fat diets to induce hyperlipidemia. The body weight of mice was measured every day,and the dose was adjusted according to the weight. On the 40 th day,the mice were decapitated and the TC,TG,HDL-C and LDLC were detected. Changes of weight and viscera indexes were observed. The hypoglycemic activity of polysaccharide was investigated by animal experiments. Results: Compared with model group,the TC,TG and LDL-C of serum in the flavonoid groups were deceased significantly( P < 0. 05) and HDL-C was increased significantly( P < 0. 05). The total flavonoids from pomace of Fructus Hippophae group decreased fasting blood glucose values significantly( P < 0. 05). Conclusion: The total flavonoids from pomace of Fructus Hippophae have hypolipidemic and hypoglycemic effects.
Objective: To study the hypolipidemic and hypoglycemic effects of total flavonoids from pomace of Fructus Hippophae.Methods: A total of 72 Kunming male mice were randomly divided into six groups( n = 12 in each group) : normal group,model group,simvastatin group,low-dose flavonoid group,middle-dose flavonoid group and high-dose flavonoid group. Except the blank control group,the mice were fed with high fat diets to induce hyperlipidemia. The body weight of mice was measured every day,and the dose was adjusted according to the weight. On the 40 th day,the mice were decapitated and the TC,TG,HDL-C and LDLC were detected. Changes of weight and viscera indexes were observed. The hypoglycemic activity of polysaccharide was investigated by animal experiments. Results: Compared with model group,the TC,TG and LDL-C of serum in the flavonoid groups were deceased significantly( P < 0. 05) and HDL-C was increased significantly( P < 0. 05). The total flavonoids from pomace of Fructus Hippophae group decreased fasting blood glucose values significantly( P < 0. 05). Conclusion: The total flavonoids from pomace of Fructus Hippophae have hypolipidemic and hypoglycemic effects.
引文
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[12]白生文,汤超,田京,等.沙棘果渣总黄酮提取工艺及抗氧化活性分析[J].食品科学,2015,36(10):59-64.
[13]赵玉珍.沙棘中黄酮类化合物及其药用价值[J].沙棘,1997,10(1):39-41.
[14]段金廒,宿树兰,郭盛,等.中药资源产业化过程废弃物的产生及其利用策略与资源化模式[J],中草药,2013,44(20):2787-2797.
[15]李峰,王娜,张师愚,等.中药药渣的综合利用及其研究进展[J],中国医药工业杂志,2016,47(10):1322-1326.
[2]利毛才让,索有瑞.正交设计优化沙棘果中总酚酸的提取工艺[J].西北药学杂志,2011,26(2):82-84.
[3]Sadik CD,Sies H,Schewe T.Inhibition of 15-lipoxygenases by flavonoids:structure-activity relations and mode of action[J].Biochem Pharmacol,2003,65(5):773-781.
[4]王秉文,李小安,康军,等.沙棘叶中黄酮的抗衰老作用的实验研究[J].沙棘,1998,11(2):26-31.
[5]Woodman OL,Meeker WF,Boujaoude M.Vasorelaxant and antioxidant activity of flavonols and flavones:structure-activity relationships[J].J Cardiovasc Pharmacol,2005,46(3):302-309.
[6]Kim YW,Hackett JC,Brueggemeier RW.Synthesis and aromatase inhibitory activity of novel pyridine-containing isoflavones[J].J Med Chem,2004,47(16):4032-4040.
[7]高锦明,李芸生.沙棘黄酮化学研究的进展[J].沙棘,1998,11(2):34-40.
[8]高锦明,李芸生,张鞍灵,等.沙棘黄酮化学研究的进展[J].沙棘,1998,11(2):34-39.
[9]张氽,阚建全,陈宗道.生物类黄酮抗癌作用研究进展[J].中国食品添加剂,2003,13(3):17-20.
[10]刘莉华,宛晓春,李大祥.黄酮类化合物抗氧化活性构效关系的研究进展(综述)[J].安徽农业大学学报,2002,29(3):265-270.
[11]曹父,陈广仁,王爱国.沙棘黄酮类化合物及其生理功能探究[J].饮料工业,2003,6(6):5-9.
[12]白生文,汤超,田京,等.沙棘果渣总黄酮提取工艺及抗氧化活性分析[J].食品科学,2015,36(10):59-64.
[13]赵玉珍.沙棘中黄酮类化合物及其药用价值[J].沙棘,1997,10(1):39-41.
[14]段金廒,宿树兰,郭盛,等.中药资源产业化过程废弃物的产生及其利用策略与资源化模式[J],中草药,2013,44(20):2787-2797.
[15]李峰,王娜,张师愚,等.中药药渣的综合利用及其研究进展[J],中国医药工业杂志,2016,47(10):1322-1326.