高温胁迫对不同耐性大豆品种生理生化的影响
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摘要
为了探索高温胁迫对不同耐性大豆品种的影响,在不同生育时期对不同耐热性大豆品种进行高温胁迫处理,研究其对大豆品种生理特性的影响以及各生理指标与单株粒重的相关性。结果表明,高温胁迫下大豆叶片中可溶性糖含量、叶绿素a/b值呈下降趋势,MDA含量和SOD酶活性呈上升趋势。苗期、开花期和鼓粒期40±2℃高温胁迫使长农17可溶性糖含量分别降低43. 34%、28. 09%和34. 25%,使锦州4-1分别降低59. 03%、58. 66%和45. 68%。苗期40±2℃高温胁迫下叶绿素a/b值下降最为明显,锦州4-1叶绿素a/b值下降幅度(40. 55%)高于长农17叶绿素a/b值下降幅度(34. 40%)。开花期MDA含量变化幅度最大,40±2℃高温胁迫下,锦州4-1的MDA含量增加幅度(135. 94%)高于长农17的MDA含量增加幅度(114. 86%)。在开花期和鼓粒期高温胁迫下叶片脯氨酸含量呈增加趋势,开花期两品种差异最为明显,40±2℃高温胁迫使长农17脯氨酸含量增加了113. 84%,而使锦州4-1脯氨酸含量增加19. 61%。在苗期和开花期随胁迫温度的增加ASA含量、POD酶活性呈下降趋势,而在鼓粒期高温胁迫下则呈增加趋势。单株粒重与大豆叶片可溶性糖含量和叶绿素a/b值呈极显著正相关,与MDA含量呈显著负相关。综上可得,高温胁迫下耐热品种长农17的可溶性糖含量、叶绿素a/b值及SOD酶活性均高于热敏感品种锦州4-1,MDA含量低于热敏感品种锦州4-1。35±2℃、40±2℃高温胁迫下,耐热性品种长农17的脯氨酸含量、ASA含量、SOD以及POD酶活性水平高于热敏感品种锦州4-1,耐热品种长农17在高温胁迫下仍能保持较高的单株粒重,且品种间差异显著。
In order to explore the effects of high temperature stress on different heat-resistant soybean,in this study,differentheat-resistant soybean varieties were treated under high temperature stress at different growth stages,and the physiological andbiochemical traits of soybean varieties and the correlation between physiological characters and seed weight per plant werestudied. The results showed that soluble sugar content,Chl a/b value decreased,MDA content and SOD activity increasedwith the increase of stress temperature at different growth stages. Under the treatment of 40 ± 2℃ in seedling stage,floweringstage and seed-filling stage,the content of soluble sugar of Changnong 17 decreased by 43. 34%,28. 09% and 34. 25% re-spectively,and the content of soluble sugar of of Jinzhou 4-1 decreased by 59. 03%,58. 66% and 45. 68% respectively. TheChl a/b value under the treatment of 40 ± 2℃ at seeding stage decreased most obviously. The decrease of Chl a/b value ofJinzhou 4-1(40. 55%) exceeded the decrease of Chl a/b value of Changnong 17(34. 40%). The increase of MDA contentunder the treatment of 40 ± 2℃ at the flowering stage was the highest. The increase of MDA content of Jinzhou 4-1(135. 94%) exceeded the increase of MDA content of Changnong 17(114. 86%). The content of proline in leaves increasedwith the increase of stress temperature at flowering stage and seed-filling stage,and the most obvious difference between thetwo varieties under the treatment of 40 ± 2℃ was that the proline content increased by 113. 84% for Changnong 17 and by19. 61% for Jinzhou 4-1. The activity of POD and ASA content decreased with the increase of stress temperature at seedlingstage and flowering stage,and increased at seed filling stage. Grain weight per plant was extremely a significant positive corre-lation with soluble sugar content and Chl a/b value,and significant negative correlation with MDA content. In conclusion,thesoluble sugar content,Chl a/b value and SOD activity of heat-resistant variety Changnong 17 were all higher than those ofheat-sensitive variety Jinzhou 4-1,and the MDA content of heat-resistant variety Changnong 17 was lower than that of heat-sensitive variety Jinzhou 4-1. Proline content,ASA content,SOD activity and POD activity of Changnong 17 were still higherthan those of Jinzhou 4-1 under the treatment of 35 ± 2℃,40 ± 2℃. Seed weight per plant of heat-resistant variety Changnong17 still kept a higher level,and there was a significant difference between the varieties.
In order to explore the effects of high temperature stress on different heat-resistant soybean,in this study,differentheat-resistant soybean varieties were treated under high temperature stress at different growth stages,and the physiological andbiochemical traits of soybean varieties and the correlation between physiological characters and seed weight per plant werestudied. The results showed that soluble sugar content,Chl a/b value decreased,MDA content and SOD activity increasedwith the increase of stress temperature at different growth stages. Under the treatment of 40 ± 2℃ in seedling stage,floweringstage and seed-filling stage,the content of soluble sugar of Changnong 17 decreased by 43. 34%,28. 09% and 34. 25% re-spectively,and the content of soluble sugar of of Jinzhou 4-1 decreased by 59. 03%,58. 66% and 45. 68% respectively. TheChl a/b value under the treatment of 40 ± 2℃ at seeding stage decreased most obviously. The decrease of Chl a/b value ofJinzhou 4-1(40. 55%) exceeded the decrease of Chl a/b value of Changnong 17(34. 40%). The increase of MDA contentunder the treatment of 40 ± 2℃ at the flowering stage was the highest. The increase of MDA content of Jinzhou 4-1(135. 94%) exceeded the increase of MDA content of Changnong 17(114. 86%). The content of proline in leaves increasedwith the increase of stress temperature at flowering stage and seed-filling stage,and the most obvious difference between thetwo varieties under the treatment of 40 ± 2℃ was that the proline content increased by 113. 84% for Changnong 17 and by19. 61% for Jinzhou 4-1. The activity of POD and ASA content decreased with the increase of stress temperature at seedlingstage and flowering stage,and increased at seed filling stage. Grain weight per plant was extremely a significant positive corre-lation with soluble sugar content and Chl a/b value,and significant negative correlation with MDA content. In conclusion,thesoluble sugar content,Chl a/b value and SOD activity of heat-resistant variety Changnong 17 were all higher than those ofheat-sensitive variety Jinzhou 4-1,and the MDA content of heat-resistant variety Changnong 17 was lower than that of heat-sensitive variety Jinzhou 4-1. Proline content,ASA content,SOD activity and POD activity of Changnong 17 were still higherthan those of Jinzhou 4-1 under the treatment of 35 ± 2℃,40 ± 2℃. Seed weight per plant of heat-resistant variety Changnong17 still kept a higher level,and there was a significant difference between the varieties.
引文
[1]秦大河,罗勇.全球气候变化的原因和未来变化趋势[J].科学对社会的影响,2008(2):16-21.(Qin D H,Luo Y. Thecauses and trends of global climate change[J]. Impact of Scienceon Society,2008(2):16-21.)
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[5]包刚,覃志豪,周义,等.气候变化对中国农业生产影响的模拟评价进展[J].中国农学通报,2012,28(2):303-307.(BaoG,Qin Z H,Zhou Y,et al. Advance of evaluation of climate im-pact on crop yield[J]. Chinese Agriculture Science Bulletin,2012,28(2):303-307.)
[6]张桂莲,陈立云,张顺堂,等.抽穗开花期高温对水稻剑叶理化特性的影响[J].中国农业科学,2007,40(7):1345-1352.(Zhang G L,Chen L Y,Zhang S T,et al. Effects of high temper-ature on physiological and biochemical characteristics in flag leaf ofrice during heading and flowering period[J]. Scientia AgriculturaSinica,2007,40(7):1345-1352.)
[7]段骅.高温与干旱对水稻产量和品质的影响及其生理机制[D].扬州:扬州大学,2013.(Duan H. Effect of high tempera-ture and soil drying on the yield quality and quantity of rice and itsphysiological mechanism[D]. Yangzhou:Yangzhou University,2013.)
[8]夏天舒,卞景阳,谭贺,等.垦丰11大豆品种高温胁迫响应研究[J].黑龙江农业科学,2010(12):23-24.(Xia T S,BianJ Y,Tan H,et al. Response to high temperature stress of Kenfeng11 soybean varieties[J]. Heilongjiang Agricultural Sciences,2010(12):23-24.)
[9] Amjad H,Madiha G,Nayyer I. Heat stress-induced cell death,changes in antioxidants,lipid peroxidation,and protease activityin wheat leaves[J]. Plant Growth Regulation,2012,31(3):283-291.
[10]陈秀晨,王士梅,朱启升,等.水稻品种耐热性与相关生化指标的关联分析[J].农业环境科学学报,2010,29(9):1633-1639.(Chen X C,Wang S M,Zhu Q S,et al. Correlation analy-sis between high temperature resistance and biochemical index a-mong different rice varieties[J]. Journal of Agro-Environment Sci-ence,2010,29(9):1633-1639.)
[11]靳路真,王洋,张伟,等.大豆品种(系)耐热性鉴定及分级评鉴[J].中国油料作物学报,2016,38(1):77-87.(Jin L Z,Wang Y,Zhang W,et al. Grading evaluation on heat-tolerance insoybean and identification of heat-tolerant cultivars[J]. ChineseJournal Oil Crop Sciences,2016,38(1):77-87.)
[12]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.(Li H C. Principles and techniques of plant physi-ological and biochemical experiments[M]. Beijing:AdvancedEducation Press,2000.)
[13]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.(Zou Q. Experimental instruction in plant physiology[M].Beijing:China Agricultural Press,2000.)
[14]李玲.植物生理学模块实验指导[M].北京:科学出版社,2009.(Li L. Experimental guidance on plant physiology module[M]. Beijing:Science Press,2009.)
[15]王忠.植物生理学[M].北京:中国农业出版社,2000.(Wang Z.Plant physiology[M]. Beijing:China Agricultural Press,2000.)
[16]王孝官,李树德.冷胁迫对番茄中ABA、可溶性糖和呼吸强度的影响[J].园艺学报,1998,25(1):56-60.(Wang X G,Li SD. Effects of cold stress on ABA,soluble sugar and respiratory in-tensity in tomato[J]. Acta Horticulturae Sinica,1998,25(1):56-60.)
[17]刘媛媛,滕中华,王三根,等.高温胁迫对水稻可溶性糖及膜保护酶的影响研究[J].西南大学学报(自然科学版),2008,30(2):59-63.(Liu Y Y,Teng Z H,Wang S G,et al. Effect ofhigh temperature stress on soluble sugar and membrane protectiveenzyme in rice[J]. Journal of Southwest University(Natural Sci-ence Edition),2008,30(2):59-63.)
[18]赵森,于江辉,肖国樱.高温胁迫对爪哇稻剑叶光合特性和渗透调节物质的影响[J].生态环境学报,2013,22(1):110-115.(Zhao S,Yu J H,Xiao G Y. Effects of high temperaturestress on the photosynthesis and osmoregulation substance of flagleaves in Oryza stavia L. ssp. Javanica[J]. Eclolhy and Environ-ment Sciences,2013,22(1):110-115.)
[19]全先庆,张渝洁,单雷,等.高等植物脯氨酸代谢研究进展[J].生物技术通讯,2007(1):14-18.(Quan X Q,Zhang Y J,Shan L,et al. Advances in proline metabolism research of higherpants[J]. Biotechnology Bulletin,2007(1):14-18.)
[20]陈因,方大惟.外源脯氨酸对受Na Cl胁迫的蓝藻固氮活性的影响[J].植物生理学通讯,1992,28(4):254-258.(Chen Y,Fang D W. Effects of exogenous proline on nitrogen fixation activi-ty of cyanobacteria subjected to Na Cl stress[J]. Plant PhysiologyCommunications,1992,28(4):254-258.)
[21]沙汉景.外源脯氨酸对盐胁迫下水稻耐盐性的影响[D].哈尔滨:东北农业大学,2013.(Sha H J. Effect of exogenous pro-line on the salt-tolerance of rice[D]. Harbin:Northeast Agricul-tural University,2013.)
[22]李珍珍,韩阳.抗坏血酸对小麦种子老化及幼苗脂质过氧化的影响[J].辽宁大学学报(自然科学版),2000,23(5):56-61.(Li Z Z,Han Y. Effects of ascorbic acid on aging of wheatseeds and lipid peroxidation of seedlings[J]. Journal of LiaoningUniversity(Natural Science Edition),2000,23(5):56-61.)
[23]姜春明,尹燕枰,刘霞,等.不同耐热性小麦品种旗叶膜脂过氧化和保护酶活性对花后高温胁迫的响应[J].作物学报,2007,33(11):143-148.(Jiang C M,Yin Y P,Liu X,et al.Response of flag leaf lipid peroxidation and protective enzyme ac-tivity of wheat cultivars with different heat tolerance to high tem-perature stress after anthesis[J]. Acta Agronomica Sinica,2007,33(11):143-148.)
[24]谢晓金,李秉柏,申双和,等.高温胁迫对扬稻6号剑叶生理特性的影响[J].中国农业气象,2009,30(1):84-87.(Xie XJ,Lin B B,Shen S H,et al. Influence of high temperature stresson some physiological characteristics of flag leaves of rice varietyYangdao 6[J]. Chinese Journal of Agrometeorology,2009,30(1):84-87.)
[25]卢琼琼,宋新山,严登华.高温胁迫对大豆幼苗生理特性的影响[J].河南师范大学学报(自然科学版),2012,40(1):112-124.(Lu Q Q,Song X S,Yan D H. Effects of high tempera-ture stress on physiological characteristics of soybean seedlings[J]. Journal of Henan Normal University(Natural Science Edi-tion),2012,40(1):112-124.)
[26] Liu X,Huang B. Heat stress injury in relation to membrane lipidperoxidation in creeping bent grass[J]. Crop Science,2000,40(2):503-510.
[27]李万成,朱启升,王云生,等.高温胁迫条件下水稻生理生化指标与产量性状的相关性研究[J].中国农学通报,2013,29(9):5-10.(Li W C,Zhu Q S,Wang Y S,et al. The relation-ship between physiological and biochemical indexes and the yieldcharacteristics of rice under high temperature stress[J]. ChineseAgricultural Science Bulletin,2013,29(9):5-10.)
[28]马德华,庞金安,霍振荣,等.黄瓜对不同温度逆境的抗性研究[J].中国农业科学,1999,32(5):28-35.(Ma D H. Pang JA,Huo Z R,et al. Research on cucumber resistance to differenttemperature adversity[J]. Scientia Agricultura Sinica,1999,32(5):28-35.)
[2]王馥堂,赵宗慈,王石力.气候变化对农业生态的影响[M].北京:气象出版社,2003.(Wang F T,Zhao Z C,Wang S L.The impact of climate change on agricultural ecology[M]. Bei-jing:China Meteorological Press,2003.)
[3] Martineau J R,Specht J E. Temperature tolerance in soybeans[J]. Crop Science,1979,19(1):75-81.
[4]艾青,牟同敏.水稻耐热性研究进展[J].湖北农业科学,2008,47(1):107-111.(Ai Q,Mu T M. Research progress ofrice heat tolerance[J]. Hubei Agricultural Sciences,2008,47(1):107-111.)
[5]包刚,覃志豪,周义,等.气候变化对中国农业生产影响的模拟评价进展[J].中国农学通报,2012,28(2):303-307.(BaoG,Qin Z H,Zhou Y,et al. Advance of evaluation of climate im-pact on crop yield[J]. Chinese Agriculture Science Bulletin,2012,28(2):303-307.)
[6]张桂莲,陈立云,张顺堂,等.抽穗开花期高温对水稻剑叶理化特性的影响[J].中国农业科学,2007,40(7):1345-1352.(Zhang G L,Chen L Y,Zhang S T,et al. Effects of high temper-ature on physiological and biochemical characteristics in flag leaf ofrice during heading and flowering period[J]. Scientia AgriculturaSinica,2007,40(7):1345-1352.)
[7]段骅.高温与干旱对水稻产量和品质的影响及其生理机制[D].扬州:扬州大学,2013.(Duan H. Effect of high tempera-ture and soil drying on the yield quality and quantity of rice and itsphysiological mechanism[D]. Yangzhou:Yangzhou University,2013.)
[8]夏天舒,卞景阳,谭贺,等.垦丰11大豆品种高温胁迫响应研究[J].黑龙江农业科学,2010(12):23-24.(Xia T S,BianJ Y,Tan H,et al. Response to high temperature stress of Kenfeng11 soybean varieties[J]. Heilongjiang Agricultural Sciences,2010(12):23-24.)
[9] Amjad H,Madiha G,Nayyer I. Heat stress-induced cell death,changes in antioxidants,lipid peroxidation,and protease activityin wheat leaves[J]. Plant Growth Regulation,2012,31(3):283-291.
[10]陈秀晨,王士梅,朱启升,等.水稻品种耐热性与相关生化指标的关联分析[J].农业环境科学学报,2010,29(9):1633-1639.(Chen X C,Wang S M,Zhu Q S,et al. Correlation analy-sis between high temperature resistance and biochemical index a-mong different rice varieties[J]. Journal of Agro-Environment Sci-ence,2010,29(9):1633-1639.)
[11]靳路真,王洋,张伟,等.大豆品种(系)耐热性鉴定及分级评鉴[J].中国油料作物学报,2016,38(1):77-87.(Jin L Z,Wang Y,Zhang W,et al. Grading evaluation on heat-tolerance insoybean and identification of heat-tolerant cultivars[J]. ChineseJournal Oil Crop Sciences,2016,38(1):77-87.)
[12]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.(Li H C. Principles and techniques of plant physi-ological and biochemical experiments[M]. Beijing:AdvancedEducation Press,2000.)
[13]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.(Zou Q. Experimental instruction in plant physiology[M].Beijing:China Agricultural Press,2000.)
[14]李玲.植物生理学模块实验指导[M].北京:科学出版社,2009.(Li L. Experimental guidance on plant physiology module[M]. Beijing:Science Press,2009.)
[15]王忠.植物生理学[M].北京:中国农业出版社,2000.(Wang Z.Plant physiology[M]. Beijing:China Agricultural Press,2000.)
[16]王孝官,李树德.冷胁迫对番茄中ABA、可溶性糖和呼吸强度的影响[J].园艺学报,1998,25(1):56-60.(Wang X G,Li SD. Effects of cold stress on ABA,soluble sugar and respiratory in-tensity in tomato[J]. Acta Horticulturae Sinica,1998,25(1):56-60.)
[17]刘媛媛,滕中华,王三根,等.高温胁迫对水稻可溶性糖及膜保护酶的影响研究[J].西南大学学报(自然科学版),2008,30(2):59-63.(Liu Y Y,Teng Z H,Wang S G,et al. Effect ofhigh temperature stress on soluble sugar and membrane protectiveenzyme in rice[J]. Journal of Southwest University(Natural Sci-ence Edition),2008,30(2):59-63.)
[18]赵森,于江辉,肖国樱.高温胁迫对爪哇稻剑叶光合特性和渗透调节物质的影响[J].生态环境学报,2013,22(1):110-115.(Zhao S,Yu J H,Xiao G Y. Effects of high temperaturestress on the photosynthesis and osmoregulation substance of flagleaves in Oryza stavia L. ssp. Javanica[J]. Eclolhy and Environ-ment Sciences,2013,22(1):110-115.)
[19]全先庆,张渝洁,单雷,等.高等植物脯氨酸代谢研究进展[J].生物技术通讯,2007(1):14-18.(Quan X Q,Zhang Y J,Shan L,et al. Advances in proline metabolism research of higherpants[J]. Biotechnology Bulletin,2007(1):14-18.)
[20]陈因,方大惟.外源脯氨酸对受Na Cl胁迫的蓝藻固氮活性的影响[J].植物生理学通讯,1992,28(4):254-258.(Chen Y,Fang D W. Effects of exogenous proline on nitrogen fixation activi-ty of cyanobacteria subjected to Na Cl stress[J]. Plant PhysiologyCommunications,1992,28(4):254-258.)
[21]沙汉景.外源脯氨酸对盐胁迫下水稻耐盐性的影响[D].哈尔滨:东北农业大学,2013.(Sha H J. Effect of exogenous pro-line on the salt-tolerance of rice[D]. Harbin:Northeast Agricul-tural University,2013.)
[22]李珍珍,韩阳.抗坏血酸对小麦种子老化及幼苗脂质过氧化的影响[J].辽宁大学学报(自然科学版),2000,23(5):56-61.(Li Z Z,Han Y. Effects of ascorbic acid on aging of wheatseeds and lipid peroxidation of seedlings[J]. Journal of LiaoningUniversity(Natural Science Edition),2000,23(5):56-61.)
[23]姜春明,尹燕枰,刘霞,等.不同耐热性小麦品种旗叶膜脂过氧化和保护酶活性对花后高温胁迫的响应[J].作物学报,2007,33(11):143-148.(Jiang C M,Yin Y P,Liu X,et al.Response of flag leaf lipid peroxidation and protective enzyme ac-tivity of wheat cultivars with different heat tolerance to high tem-perature stress after anthesis[J]. Acta Agronomica Sinica,2007,33(11):143-148.)
[24]谢晓金,李秉柏,申双和,等.高温胁迫对扬稻6号剑叶生理特性的影响[J].中国农业气象,2009,30(1):84-87.(Xie XJ,Lin B B,Shen S H,et al. Influence of high temperature stresson some physiological characteristics of flag leaves of rice varietyYangdao 6[J]. Chinese Journal of Agrometeorology,2009,30(1):84-87.)
[25]卢琼琼,宋新山,严登华.高温胁迫对大豆幼苗生理特性的影响[J].河南师范大学学报(自然科学版),2012,40(1):112-124.(Lu Q Q,Song X S,Yan D H. Effects of high tempera-ture stress on physiological characteristics of soybean seedlings[J]. Journal of Henan Normal University(Natural Science Edi-tion),2012,40(1):112-124.)
[26] Liu X,Huang B. Heat stress injury in relation to membrane lipidperoxidation in creeping bent grass[J]. Crop Science,2000,40(2):503-510.
[27]李万成,朱启升,王云生,等.高温胁迫条件下水稻生理生化指标与产量性状的相关性研究[J].中国农学通报,2013,29(9):5-10.(Li W C,Zhu Q S,Wang Y S,et al. The relation-ship between physiological and biochemical indexes and the yieldcharacteristics of rice under high temperature stress[J]. ChineseAgricultural Science Bulletin,2013,29(9):5-10.)
[28]马德华,庞金安,霍振荣,等.黄瓜对不同温度逆境的抗性研究[J].中国农业科学,1999,32(5):28-35.(Ma D H. Pang JA,Huo Z R,et al. Research on cucumber resistance to differenttemperature adversity[J]. Scientia Agricultura Sinica,1999,32(5):28-35.)