外源SA对盐碱胁迫下葡萄幼苗生长与光系统的影响
|
摘要
水杨酸(SA)是植物体内的一种重要激素,在多种逆境下都发挥作用。本试验以中等耐盐碱葡萄品种‘Dechaunac(Seibel 9549)’水培幼苗为材料,研究外源水杨酸对葡萄幼苗在盐碱胁迫下的生长和光系统活性的影响。研究表明:葡萄幼苗在0.1mol·L~(-1) NaHCO3盐碱胁迫后,长势变差,株高、鲜质量下降,与对照相比,叶绿素含量、净光合速率、气孔导度、蒸腾速率均显著下降,而0.1mmol·L~(-1)外源SA处理能有效缓解这种下降。对光系统Ⅱ荧光参数的研究发现,盐碱胁迫后指标Wk显著升高,说明放氧复合体(OEC)有明显损伤;有活性反应中心(RC/CSo)数量下降;电子传递活性受到抑制(φEo显著下降),而SA处理能显著缓解对OEC和有活性反应中心的损伤,加快电子传递,同时也减缓盐碱胁迫对光系统Ⅰ的损伤。从以上结果得出结论:0.1mmol·L~(-1)外源SA处理能促进盐碱胁迫下葡萄幼苗的生长,是因为缓解了盐碱胁迫对光系统的损伤作用,促进了电子传递,从而提高光合速率。
Salicylic acid(SA)is an important hormone in plants,which can alleviate the damage of many kinds of adversity.In this study,the hydroponic seedlings of medium saline-tolerant grape variety‘Dechaunac(Seibel 9549)'were used as materials and the effects of exogenous SA on the growth and photosystems of grape seedlings under saline-alkali stress were studied.The results showed that after the saline-alkali stress with 0.1 mol·L-1 NaHCO3 treated,the growth of grape seedlings became worse,plant height and fresh weight,chlorophyll content,net photosynthetic rate,stomatal conductance,transpiration rate were decreased significantly compared with the control.But the treatment with 0.1 mmol·L~(-1) exogenous SA could effectively alleviate this decline.The fluorescence parameters of photosystemⅡunder saline-alkali stress were analyzed.It was found that Wkincreased sharply,indicating the oxygen evolution complex(OEC)was damaged,density of RCs(RC/CSo)decreased clearly and electron transport activity inhibited(φEo decreased evidently).But SA treatment significantly alleviated the damage to OEC and reaction centers,and accelerated electron transfer rate and attenuate the damage of photosystemⅠat the same time.From above results we arrived at a conclusion that the treatment with 0.1 mmol·L~(-1) exogenous SA could promoted the growth of grape seedlings under salt-alkali stress because SA treatment could significantly alleviate the damage to photosystem and increased photosynthetic rate.
Salicylic acid(SA)is an important hormone in plants,which can alleviate the damage of many kinds of adversity.In this study,the hydroponic seedlings of medium saline-tolerant grape variety‘Dechaunac(Seibel 9549)'were used as materials and the effects of exogenous SA on the growth and photosystems of grape seedlings under saline-alkali stress were studied.The results showed that after the saline-alkali stress with 0.1 mol·L-1 NaHCO3 treated,the growth of grape seedlings became worse,plant height and fresh weight,chlorophyll content,net photosynthetic rate,stomatal conductance,transpiration rate were decreased significantly compared with the control.But the treatment with 0.1 mmol·L~(-1) exogenous SA could effectively alleviate this decline.The fluorescence parameters of photosystemⅡunder saline-alkali stress were analyzed.It was found that Wkincreased sharply,indicating the oxygen evolution complex(OEC)was damaged,density of RCs(RC/CSo)decreased clearly and electron transport activity inhibited(φEo decreased evidently).But SA treatment significantly alleviated the damage to OEC and reaction centers,and accelerated electron transfer rate and attenuate the damage of photosystemⅠat the same time.From above results we arrived at a conclusion that the treatment with 0.1 mmol·L~(-1) exogenous SA could promoted the growth of grape seedlings under salt-alkali stress because SA treatment could significantly alleviate the damage to photosystem and increased photosynthetic rate.
引文
[1]Balal RM,Ashraf MY,Khan MM.Influence of salt stress on growth and biochemical parameters of citrus rootstocks[J].Pakistan Journal of Botany,2011,43(4):2135-2141
[2]Yang JY,Zheng W,Tian Y,et al.Effects of various mixed salt-alkaline stresses on growth,photosynthesis,and photosynthetic pigment concentrations of Medicagoruthenica seedlings[J].Photosynthetica,2011,49(2):275-284
[3]Liu J,Shi DC.Photosynthesis,chlorophyll fluorescence,inorganic ion and organic acid accumulations of sunflower in responses to salt and salt-alkaline mixed stress[J].Photosynthetica,2010,48(1):127-134
[4]Munns R,Tester M.Mechanisms of salinity tolerance[J].Annu Rev Plant Biol,2008,59(1):651-681
[5]Yang CW,Chong JN,Li CY,et al.Osmotic adjustment and ion balance traits of an alkali resistant halophyte Kochia sieversiana during adaptation to salt and alkali conditions[J].Plant and Soil,2007,294(1/2):263-276
[6]Aftab T,Khan MMA,da Silva JAT,et al.Role of salicylic acid in promoting salt stress tolerance and enhanced artemisinin production in artemisiaannua L[J].Journal of Plant Growth Regulation,2011,30(4):425-435
[7]Liu W,Zhang Y,Yuan X,et al.Exogenous salicylic acid improves salinity tolerance of Nitrariatangutorum[J].Russian Journal of Plant Physiology,2016,63(1):132-142
[8]Khan W,Prithiviraj B,Smith DL.Photosynthetic responses of corn and soybean to foliar application of salicylates[J].J Plant Physiol,2003,160(5):485-492
[9]Janda K,Hideg E,Szalai G,et al.Salicylic acid may indirectly influence the photosynthetic electron transport[J].J Plant Physiol,2012,169(10):971-978
[10]沙汉景,刘化龙,王敬国,等.水杨酸调控作物耐盐性生理机制[J].东北农业大学学报,2017,48(3):80-88
[11]杨德翠,刘超,盖树鹏,等.牡丹柱枝孢叶斑病(Cylindrocladiumcanadense)对叶片光合系统功能的影响[J].园艺学报,2013,40(3):515-522
[12]李鹏民,高辉远,Reto JStrasser.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566
[13]王振兴,吕海燕,秦红艳,等.盐碱胁迫对山葡萄光合特性及生长发育的影响[J].西北植物学报,2017,37(2):339-345
[14]Farquhar GD,Sharkey TD.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33(1):317-345
[15]王艳杰,薛达元,彭羽.盐碱胁迫对两个葡萄品种光合作用-光响应特性的影响[J].北方园艺,2011(16):30-36
[16]公婷婷,冯金朝,薛达元,等.盐碱胁迫对两个品种葡萄光合特性的影响[J].北方园艺,2013(19):5-9
[17]彭浩,宋文路,王晓强,等.水杨酸与植物抗逆性关系研究进展[J].园艺与种苗,2016,36(2):70-74,78
[18]宋士清,郭世荣,尚庆茂,等.外源SA对盐胁迫下黄瓜幼苗的生理效应[J].园艺学报,2006,33(1):68-72
[19]张志刚,尚庆茂.水杨酸、壳聚糖对盐胁迫下黄瓜叶片光合参数的调节作用[J].西北农业学报,2010,19(3):174-178
[20]Liu S,Dong YJ,Xu LL,et al.Effects of foliar applications of nitric oxide and salicylic acid on salt-induced changes in photosynthesis and antioxidative metabolism of cotton seedlings[J].Plant Growth Regulation,2014,73(1):67-78
[21]李婧男,刘强,李升.水杨酸对盐胁迫下沙冬青幼苗抗氧化酶活性及PSⅡ光化学效率的影响[J].华南农业大学学报,2010,31(1):42-46
[22]Qiu C,Ji W,GuoY.Effects of high temperature and strong light on chlorophyll fluorescence,the D1protein,and Deg1protease in Satsuma mandarin,and the protective role of salicylic acid[J].ActaEcologicaSinica,2011,31(3):3802-3810
[23]Janda T,Gondor OK,Yordanova R,et al.Salicylic acid and photosynthesis:signalling and effects[J].ActaPhysiologiaePlantarum,2014,36(10):2537-2546
[2]Yang JY,Zheng W,Tian Y,et al.Effects of various mixed salt-alkaline stresses on growth,photosynthesis,and photosynthetic pigment concentrations of Medicagoruthenica seedlings[J].Photosynthetica,2011,49(2):275-284
[3]Liu J,Shi DC.Photosynthesis,chlorophyll fluorescence,inorganic ion and organic acid accumulations of sunflower in responses to salt and salt-alkaline mixed stress[J].Photosynthetica,2010,48(1):127-134
[4]Munns R,Tester M.Mechanisms of salinity tolerance[J].Annu Rev Plant Biol,2008,59(1):651-681
[5]Yang CW,Chong JN,Li CY,et al.Osmotic adjustment and ion balance traits of an alkali resistant halophyte Kochia sieversiana during adaptation to salt and alkali conditions[J].Plant and Soil,2007,294(1/2):263-276
[6]Aftab T,Khan MMA,da Silva JAT,et al.Role of salicylic acid in promoting salt stress tolerance and enhanced artemisinin production in artemisiaannua L[J].Journal of Plant Growth Regulation,2011,30(4):425-435
[7]Liu W,Zhang Y,Yuan X,et al.Exogenous salicylic acid improves salinity tolerance of Nitrariatangutorum[J].Russian Journal of Plant Physiology,2016,63(1):132-142
[8]Khan W,Prithiviraj B,Smith DL.Photosynthetic responses of corn and soybean to foliar application of salicylates[J].J Plant Physiol,2003,160(5):485-492
[9]Janda K,Hideg E,Szalai G,et al.Salicylic acid may indirectly influence the photosynthetic electron transport[J].J Plant Physiol,2012,169(10):971-978
[10]沙汉景,刘化龙,王敬国,等.水杨酸调控作物耐盐性生理机制[J].东北农业大学学报,2017,48(3):80-88
[11]杨德翠,刘超,盖树鹏,等.牡丹柱枝孢叶斑病(Cylindrocladiumcanadense)对叶片光合系统功能的影响[J].园艺学报,2013,40(3):515-522
[12]李鹏民,高辉远,Reto JStrasser.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566
[13]王振兴,吕海燕,秦红艳,等.盐碱胁迫对山葡萄光合特性及生长发育的影响[J].西北植物学报,2017,37(2):339-345
[14]Farquhar GD,Sharkey TD.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33(1):317-345
[15]王艳杰,薛达元,彭羽.盐碱胁迫对两个葡萄品种光合作用-光响应特性的影响[J].北方园艺,2011(16):30-36
[16]公婷婷,冯金朝,薛达元,等.盐碱胁迫对两个品种葡萄光合特性的影响[J].北方园艺,2013(19):5-9
[17]彭浩,宋文路,王晓强,等.水杨酸与植物抗逆性关系研究进展[J].园艺与种苗,2016,36(2):70-74,78
[18]宋士清,郭世荣,尚庆茂,等.外源SA对盐胁迫下黄瓜幼苗的生理效应[J].园艺学报,2006,33(1):68-72
[19]张志刚,尚庆茂.水杨酸、壳聚糖对盐胁迫下黄瓜叶片光合参数的调节作用[J].西北农业学报,2010,19(3):174-178
[20]Liu S,Dong YJ,Xu LL,et al.Effects of foliar applications of nitric oxide and salicylic acid on salt-induced changes in photosynthesis and antioxidative metabolism of cotton seedlings[J].Plant Growth Regulation,2014,73(1):67-78
[21]李婧男,刘强,李升.水杨酸对盐胁迫下沙冬青幼苗抗氧化酶活性及PSⅡ光化学效率的影响[J].华南农业大学学报,2010,31(1):42-46
[22]Qiu C,Ji W,GuoY.Effects of high temperature and strong light on chlorophyll fluorescence,the D1protein,and Deg1protease in Satsuma mandarin,and the protective role of salicylic acid[J].ActaEcologicaSinica,2011,31(3):3802-3810
[23]Janda T,Gondor OK,Yordanova R,et al.Salicylic acid and photosynthesis:signalling and effects[J].ActaPhysiologiaePlantarum,2014,36(10):2537-2546