HPLC法同时测定没食子提取物中没食子酸、没食子酸甲酯和没食子酸乙酯的含量
|
摘要
目的建立高效液相色谱法测定没食子提取物中三种成分的含量方法。方法色谱柱:Waters Symmetry C18(4.6 mm×250 mm,5μm);流动相:甲醇-水(25∶75)(冰乙酸调p H至4.5);流速为1.0 m L·min-1,柱温:30℃,检测波长:273 nm。结果没食子酸、没食子酸甲酯、没食子酸乙酯的回收率分别为99.79%、99.60%、99.45%;线性范围分别为0.2~3.2μg、0.002~0.16μg、0.010 8~0.54μg。结论结果准确,重复性好,可用于该产品的质量控制。
Objective To establish a method for the simultaneous determination of gallic acid,methyl gallate and ethyl gallate from gallnut extracts. Methods HPLC was used to quantitative analysis. The Waters Symmetry C18(4. 6 mm × 250 mm,5 μm) was used,and mobile phase was composed of methanol- water(25 ∶75)( adjust p H to 4. 5 with acetic acid).The detection wavelength was 273 nm at 30 ℃. The flow rate was 1. 0 m L·min- 1. Results The gallic acid,methyl gallate and ethyl gallate's spotting recovery were 99. 79%,99. 60%,99. 45%,and the linear ranges were 0. 2 ~ 3. 2 μg,0. 002 ~0. 16 μg,0. 010 8 ~ 0. 54 μg,respectively. Conclusion The results were accurate and the reproducibility was good. The method can be used for the quality control of the product.
Objective To establish a method for the simultaneous determination of gallic acid,methyl gallate and ethyl gallate from gallnut extracts. Methods HPLC was used to quantitative analysis. The Waters Symmetry C18(4. 6 mm × 250 mm,5 μm) was used,and mobile phase was composed of methanol- water(25 ∶75)( adjust p H to 4. 5 with acetic acid).The detection wavelength was 273 nm at 30 ℃. The flow rate was 1. 0 m L·min- 1. Results The gallic acid,methyl gallate and ethyl gallate's spotting recovery were 99. 79%,99. 60%,99. 45%,and the linear ranges were 0. 2 ~ 3. 2 μg,0. 002 ~0. 16 μg,0. 010 8 ~ 0. 54 μg,respectively. Conclusion The results were accurate and the reproducibility was good. The method can be used for the quality control of the product.
引文
[1]山东省药品监督管理局.山东省药材标准2002年版[M].济南:山东友谊出版社,2002:109-110.
[2]南京中医药大学.中药大辞典(上册)[M].上海:上海科学技术出版社,2006:1637-1638.
[3]热孜玩姑丽·阿不都热依木,木合塔尔·木明.HPLC法测定维吾尔药消食阿米勒努西颗粒中没食子酸的含量[J].中国民族医药杂志,2013,8(8):59-60.
[4]李松林,杨庆雄,王聪聪,等.反相高效液相色谱法同时测定香椿果中3种酚性化合物[J].安徽农业科学,2011,39(15):100-102.
[5]吴冬梅,陈笳鸿,汪咏梅,等.HPLC法测定没食子酸甲酯含量的研究[J].生物质化学工,2010,44(6):6-9.
[2]南京中医药大学.中药大辞典(上册)[M].上海:上海科学技术出版社,2006:1637-1638.
[3]热孜玩姑丽·阿不都热依木,木合塔尔·木明.HPLC法测定维吾尔药消食阿米勒努西颗粒中没食子酸的含量[J].中国民族医药杂志,2013,8(8):59-60.
[4]李松林,杨庆雄,王聪聪,等.反相高效液相色谱法同时测定香椿果中3种酚性化合物[J].安徽农业科学,2011,39(15):100-102.
[5]吴冬梅,陈笳鸿,汪咏梅,等.HPLC法测定没食子酸甲酯含量的研究[J].生物质化学工,2010,44(6):6-9.