自制柴金解郁颗粒剂抗抑郁作用的药效学研究
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摘要
目的:研究柴金解郁颗粒剂的抗抑郁作用。方法:建立药物相互作用和应激行为作用诱导的两种小鼠抑郁模型和慢性应激大鼠抑郁模型,将动物随机分为空白组、模型组、阳性对照(盐酸氟西汀)组(0.06 g·kg~(-1))、柴金解郁颗粒剂低(0.13 g·kg~(-1))、中(0.26 g·kg~(-1))、高(0.52 g·kg~(-1))剂量组。检测小鼠体温、眼睑下垂次数、运动不能动物数、甩头次数、悬尾不动时间、强迫游泳不动时间;检测大鼠糖水偏嗜度、活动次数、学习记忆时间。结果:与模型组比较,柴金解郁颗粒各剂量对小鼠给药体温、眼睑下垂次数、运动不能、甩头次数、游泳不动时间(除柴金解郁颗粒低剂量组)和悬尾不动时间的影响差异有统计学意义(P<0.05或P<0.01),以上作用均呈剂量相关性,且部分剂量组与阳性对照组差异有统计学意义(P<0.05或P<0.01)。与模型组比较,柴金解郁颗粒各剂量对大鼠的糖水偏嗜度、总活动数、爬上平台时间和进入目标象限时间影响差异均有统计学意义(P<0.05或P<0.01),以上作用均呈剂量相关性,且高剂量组与阳性对照组效果相似。结论:柴金解郁颗粒剂对小鼠和大鼠的抑郁症状有明显的改善作用。
Objective: To study the antidepressant effect of Chaijin Jieyu granules. Methods: Two kinds of mouse depression models induced by drug interaction and stress behavior were established,and a depression model of chronic stress was also established in rats. The animals were randomly divided into the blank group,the model group,the positive control group( fluoxetine hydrochloride,0. 06 g·kg~(-1)),and Chaijin Jieyu granules low( 0. 13 g·kg~(-1)),medium( 0. 26 g·kg~(-1)) and high( 0. 52 g·kg~(-1)) dose groups. The body temperature,eyelids drooping times,akinetic number,head shaking times,tail suspension time and immobility time in forced swimming were detected,and the sugar preference,activity frequency and learning and memory time were detected in rats.Results: Compared with the model group,different doses of Chaijin Jieyu granules showed significantly different effects on the body temperature,eyelid drooping times,akinetic number,head shaking times,swimming immobility time( except for Chaijin Jieyu granules low-dose group) and tail suspension time in mice( P < 0. 05 or P < 0. 01),the above effects were dose-related,and there was significant difference between the partial dose group and the positive control group( P < 0. 05 or P < 0. 01). Compared with the model group,different doses of Chaijin Jieyu granules showed statistically significant effects on the sugar preference,total activity,climbing platform time and entering target quadrant time in rats( P < 0. 05 or P < 0. 01),the above effects were dose-dependent,and the effects of high dose group were similar to those of the positive control group. Conclusion: Chaijin Jieyu granules can obviously improve the depressive symptoms of mice and rats.
Objective: To study the antidepressant effect of Chaijin Jieyu granules. Methods: Two kinds of mouse depression models induced by drug interaction and stress behavior were established,and a depression model of chronic stress was also established in rats. The animals were randomly divided into the blank group,the model group,the positive control group( fluoxetine hydrochloride,0. 06 g·kg~(-1)),and Chaijin Jieyu granules low( 0. 13 g·kg~(-1)),medium( 0. 26 g·kg~(-1)) and high( 0. 52 g·kg~(-1)) dose groups. The body temperature,eyelids drooping times,akinetic number,head shaking times,tail suspension time and immobility time in forced swimming were detected,and the sugar preference,activity frequency and learning and memory time were detected in rats.Results: Compared with the model group,different doses of Chaijin Jieyu granules showed significantly different effects on the body temperature,eyelid drooping times,akinetic number,head shaking times,swimming immobility time( except for Chaijin Jieyu granules low-dose group) and tail suspension time in mice( P < 0. 05 or P < 0. 01),the above effects were dose-related,and there was significant difference between the partial dose group and the positive control group( P < 0. 05 or P < 0. 01). Compared with the model group,different doses of Chaijin Jieyu granules showed statistically significant effects on the sugar preference,total activity,climbing platform time and entering target quadrant time in rats( P < 0. 05 or P < 0. 01),the above effects were dose-dependent,and the effects of high dose group were similar to those of the positive control group. Conclusion: Chaijin Jieyu granules can obviously improve the depressive symptoms of mice and rats.
引文
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5 韩远山,蔺晓源,邵乐,等.复方柴金解郁片抗抑郁作用的实验研究[J].湖南中医药大学学报,2017,37(1):18-21
6 韩珍,贺弋,杨俊,等.解郁方抗抑郁作用的实验研究[J].陕西中医,2008,29(9):1238-1239
7 朴颖,杨帆,周金田,等.六郁汤对行为绝望模型小鼠的抗抑郁作用[J].延边大学医学学报,2014,37(2):105-107
8 王宇阳,任晓暄,郑瑀,等.不同电针频率对慢性应激抑郁刺激模型大鼠行为学和下丘脑5-羟色胺水平的影响[J].长春中医药大学学报,2018,34(1):19-22
9 包伍叶,包哈申,哈斯额尔敦,等.灸“赫依穴”对慢性应激抑郁模型大鼠行为学及海马5-羟色胺的影响[J].中医药导报,2017,23(23):41-43
10 李旻.合欢花改善抑郁大鼠学习记忆能力的实验研究[J].中医临床研究,2017,9(22):27-28
2 祈雪丹,屠树滋,王秋娟.治疗抑郁症药物的研究进展[J].中国新药杂志,2003,12(10):810-816
3 陈建荣.半夏厚朴汤加味对行为绝望模型小鼠脾脏结构及体液免疫的影响[D].沈阳:辽宁中医药大学硕士学位论文,2010
4 畅洪昇,梁吉春,孙建宁,等.四逆散有效部位对小鼠行为绝望和药物抑郁模型的作用[J].北京中医药大学学报,2006,29(7):451-453
5 韩远山,蔺晓源,邵乐,等.复方柴金解郁片抗抑郁作用的实验研究[J].湖南中医药大学学报,2017,37(1):18-21
6 韩珍,贺弋,杨俊,等.解郁方抗抑郁作用的实验研究[J].陕西中医,2008,29(9):1238-1239
7 朴颖,杨帆,周金田,等.六郁汤对行为绝望模型小鼠的抗抑郁作用[J].延边大学医学学报,2014,37(2):105-107
8 王宇阳,任晓暄,郑瑀,等.不同电针频率对慢性应激抑郁刺激模型大鼠行为学和下丘脑5-羟色胺水平的影响[J].长春中医药大学学报,2018,34(1):19-22
9 包伍叶,包哈申,哈斯额尔敦,等.灸“赫依穴”对慢性应激抑郁模型大鼠行为学及海马5-羟色胺的影响[J].中医药导报,2017,23(23):41-43
10 李旻.合欢花改善抑郁大鼠学习记忆能力的实验研究[J].中医临床研究,2017,9(22):27-28