双酚A对稀有鮈鲫胚胎及仔鱼的急性毒性
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摘要
为探讨双酚A(bisphenol A,BPA)对稀有鮈鲫胚胎和仔鱼的毒性,利用静水暴露染毒法对稀有鮈鲫胚胎及仔鱼进行急性暴露。结果表明,在试验浓度范围内,BPA对稀有鮈鲫胚胎和仔鱼成活率均有显著影响(P<0.05)。胚胎暴露96 h后的半致死浓度(LC_(50))为10.096 mg·L~(-1);仔鱼暴露96 h后LC_(50)为4.237 mg·L~(-1)。BPA对稀有鮈鲫胚胎的毒性效应表现为孵化延迟、死亡率增加、心率下降,对仔鱼的毒性效应表现死亡率升高、心包囊水肿、心率下降、鱼鳔充气率下降。稀有鮈鲫胚胎和仔鱼对BPA毒性的敏感度存在差异,仔鱼对BPA暴露更为敏感。本研究旨在为BPA对鱼类早期发育阶段的毒性效应提供基础资料。
引文
[1] 王佳,詹平.双酚A对机体影响及其机制的研究进展[J].预防医学情报杂志,2005,21(5):541-544.
[2] 李芙蓉,蔡云朗,任慕兰.双酚A对离体人子宫肌瘤细胞增殖的影响[J].环境与健康杂志,2008,25(4):311-313.
[3] 杨丹,李丹丹,刘姗姗,等.双酚A对机体的影响及其作用机制[J].现代预防医学,2008,35(17):3280-3282,3287.
[4] POIMENOVA A,MARKAKI E,RAHIOTIS C,et al.Corticosterone-regulated actions in the rat brain are affected by perinatal exposure to low dose of bisphenol A[J].Neuroscience,2010,167(3):741-749.
[5] BRANISTE V,JOUAULT A,GAULTIER E,et al.Impact of oral bisphenol A at reference doses on intestinal barrier function and sex differences after perinatal exposure in rats[J].Proceedings of the National Academy of Sciences,2010,107(1):448-453.
[6] MORRISSEY R.The developmental toxicity of bisphenol A in rats and mice 1[J].Fundamental and Applied Toxicology,1987,8(4):571-582.
[7] 曹文宣,王剑伟.稀有鮈鲫:一种新的鱼类实验动物[J].实验动物科学与管理,2003,20(1):96-99.
[8] 熊力,马永鹏,毛思予,等.五氯酚对稀有鮈鲫胚胎毒性效应研究[J].中国环境科学,2012,32(2):337-344.
[9] 李楠,范添博,田义军,等.环境雌激素双酚A的研究进展[J].现代生物医学进展,2015,15(7):1353-1356.
[10] 任文娟,汪贞,王蕾,等.双酚A及其类似物对斑马鱼胚胎及幼鱼的毒性效应[J].生态毒理学报,2017,12(1):184-192.
[11] 杨丽华,史奇朋,周炳升.双酚A对斑马鱼幼鱼神经递质和神经行为的影响[J].生态毒理学报,2017,12(3):162-169.
[12] 鲁增辉,王志坚,石蕊.氨氮对稀有鮈鲫胚胎和幼鱼的急性毒性研究[J].西南大学学报(自然科学版),2014,36(1):47-52.
[13] 赵荧,李媛,陈永柏.双酚A和酞酸酯对鱼类内分泌干扰效应及繁殖毒性研究[J].水生态学杂志,2017,38(6):1-10.
[14] 刘慧慧,徐英江,邓旭修,等.莱州湾海域双酚A污染现状调查[J].渔业科学进展,2013,34(4):16-20.
[15] DE GASPAR I,BLANQUEZ M J,FRAILE B,et al.The hatching gland cells of trout embryos:characterisation of N-and O-linked oligosaccharides[J].Journal of Anatomy,1999,194(1):109-118.
[16] MILAN D J,PETERSON T A,RUSKIN J N,et al.Drugs that induce repolarization abnormalities cause bradycardia in zebrafish[J].Circulation,2003,107(10):1355-1358.
[17] 王志坚,鲁增辉,石萍.氨氮对稀有鮈鲫胚胎及卵黄囊期仔鱼的毒性效应研究[J].环境科学,2012,33(4):1323-1330.
[18] BATTAGLENE S C,TALBOT R B.Initial swim bladder inflation in intensively reared Australian bass larvae,Macquaria novemaculeata (Steindachner) (Perciformes:Percichthyidae)[J].Aquaculture,1990,86(4):431-442.
[19] CHAPMAN D C,HUBERT W A,JACKSON U T.Influence of access to air and of salinity on gas bladder inflation in striped bass[J].The Progressive Fish-Culturist,1988,50(1):23-27.
[20] CHATAIN B,OUNAIS-GUSCHEMANN N.Improved rate of initial swim bladder inflation in intensively reared Sparus auratus[J].Aquaculture,1990,84(3/4):345-353.
[21] CORNACCHIA J W.Studies on the development and inflation of the swim bladder in two physoclistous fish,Morone saxatilis and Sarotherodon mossambica[D].Davis:University of California,1981.
[22] BAILEY H C,DOROSHOV S I.The duration of the interval associated with successful inflation of the swimbladder in larval striped bass (Morone saxatilis)[J].Aquaculture,1995,131(1/2):135-143.
[2] 李芙蓉,蔡云朗,任慕兰.双酚A对离体人子宫肌瘤细胞增殖的影响[J].环境与健康杂志,2008,25(4):311-313.
[3] 杨丹,李丹丹,刘姗姗,等.双酚A对机体的影响及其作用机制[J].现代预防医学,2008,35(17):3280-3282,3287.
[4] POIMENOVA A,MARKAKI E,RAHIOTIS C,et al.Corticosterone-regulated actions in the rat brain are affected by perinatal exposure to low dose of bisphenol A[J].Neuroscience,2010,167(3):741-749.
[5] BRANISTE V,JOUAULT A,GAULTIER E,et al.Impact of oral bisphenol A at reference doses on intestinal barrier function and sex differences after perinatal exposure in rats[J].Proceedings of the National Academy of Sciences,2010,107(1):448-453.
[6] MORRISSEY R.The developmental toxicity of bisphenol A in rats and mice 1[J].Fundamental and Applied Toxicology,1987,8(4):571-582.
[7] 曹文宣,王剑伟.稀有鮈鲫:一种新的鱼类实验动物[J].实验动物科学与管理,2003,20(1):96-99.
[8] 熊力,马永鹏,毛思予,等.五氯酚对稀有鮈鲫胚胎毒性效应研究[J].中国环境科学,2012,32(2):337-344.
[9] 李楠,范添博,田义军,等.环境雌激素双酚A的研究进展[J].现代生物医学进展,2015,15(7):1353-1356.
[10] 任文娟,汪贞,王蕾,等.双酚A及其类似物对斑马鱼胚胎及幼鱼的毒性效应[J].生态毒理学报,2017,12(1):184-192.
[11] 杨丽华,史奇朋,周炳升.双酚A对斑马鱼幼鱼神经递质和神经行为的影响[J].生态毒理学报,2017,12(3):162-169.
[12] 鲁增辉,王志坚,石蕊.氨氮对稀有鮈鲫胚胎和幼鱼的急性毒性研究[J].西南大学学报(自然科学版),2014,36(1):47-52.
[13] 赵荧,李媛,陈永柏.双酚A和酞酸酯对鱼类内分泌干扰效应及繁殖毒性研究[J].水生态学杂志,2017,38(6):1-10.
[14] 刘慧慧,徐英江,邓旭修,等.莱州湾海域双酚A污染现状调查[J].渔业科学进展,2013,34(4):16-20.
[15] DE GASPAR I,BLANQUEZ M J,FRAILE B,et al.The hatching gland cells of trout embryos:characterisation of N-and O-linked oligosaccharides[J].Journal of Anatomy,1999,194(1):109-118.
[16] MILAN D J,PETERSON T A,RUSKIN J N,et al.Drugs that induce repolarization abnormalities cause bradycardia in zebrafish[J].Circulation,2003,107(10):1355-1358.
[17] 王志坚,鲁增辉,石萍.氨氮对稀有鮈鲫胚胎及卵黄囊期仔鱼的毒性效应研究[J].环境科学,2012,33(4):1323-1330.
[18] BATTAGLENE S C,TALBOT R B.Initial swim bladder inflation in intensively reared Australian bass larvae,Macquaria novemaculeata (Steindachner) (Perciformes:Percichthyidae)[J].Aquaculture,1990,86(4):431-442.
[19] CHAPMAN D C,HUBERT W A,JACKSON U T.Influence of access to air and of salinity on gas bladder inflation in striped bass[J].The Progressive Fish-Culturist,1988,50(1):23-27.
[20] CHATAIN B,OUNAIS-GUSCHEMANN N.Improved rate of initial swim bladder inflation in intensively reared Sparus auratus[J].Aquaculture,1990,84(3/4):345-353.
[21] CORNACCHIA J W.Studies on the development and inflation of the swim bladder in two physoclistous fish,Morone saxatilis and Sarotherodon mossambica[D].Davis:University of California,1981.
[22] BAILEY H C,DOROSHOV S I.The duration of the interval associated with successful inflation of the swimbladder in larval striped bass (Morone saxatilis)[J].Aquaculture,1995,131(1/2):135-143.