斯鲁菲亚海洋红酵母培养基优化
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摘要
[目的]比较不同的碳源、氮源、无机盐对斯鲁菲亚海洋红酵母(Rhodotorula slooffiae)菌株产量的影响,确定培养基最优组分。[方法]通过葡萄糖、酵母浸粉、硫酸铵、磷酸二氢钾4因素3水平的正交试验,确定斯鲁菲亚海洋红酵母的最优培养基。[结果]斯鲁菲亚海洋红酵母的最优培养基各成分质量分数为:葡萄糖2%,酵母浸粉0.75%,硫酸铵0.5%,磷酸二氢钾1%。接种量6%,500 m L三角锥形瓶,装液量100 m L,培养温度30℃,经过48 h培养,菌种量最大可达8.6×10~8CFU/m L,比优化前的发酵培养基产量(6.6×10~8CFU/m L)提升了30%。[结论]培养条件和工艺的优化可以显著提高海洋红酵母菌量和类胡萝卜素含量。
[Objective]The effects of different carbon sources,nitrogen sources and inorganic salts on fermentation yield of marine red yeast( Rhodotorula slooffiae) were compared to determine the optimal medium components. [Method]The optimum fermentation conditions for Rhodotorula slooffiae were determined by 4 factors and 3 levels orthogonal test.[Result]The optimum fermentation conditions for Rhodotorula slooffiae were determined: glucose 2%,yeast extract powder 0.75%,ammonium sulfate 0.5%,monopotassium phosphate 1%,and inoculation amount 6%,temperature 30 ℃,100 m L medium in 500 m L triangle bottle and fermentation time 48 h. Under above conditions,the cell biomass of marine red yeast can reach 8.6×10~8 cfu/m L,which increased by 30% as compared with the fermentation before optimization. [Conclusion]Optimization of culture conditions and process can significantly increase the amount of marine red yeast and carotenoid content.
[Objective]The effects of different carbon sources,nitrogen sources and inorganic salts on fermentation yield of marine red yeast( Rhodotorula slooffiae) were compared to determine the optimal medium components. [Method]The optimum fermentation conditions for Rhodotorula slooffiae were determined by 4 factors and 3 levels orthogonal test.[Result]The optimum fermentation conditions for Rhodotorula slooffiae were determined: glucose 2%,yeast extract powder 0.75%,ammonium sulfate 0.5%,monopotassium phosphate 1%,and inoculation amount 6%,temperature 30 ℃,100 m L medium in 500 m L triangle bottle and fermentation time 48 h. Under above conditions,the cell biomass of marine red yeast can reach 8.6×10~8 cfu/m L,which increased by 30% as compared with the fermentation before optimization. [Conclusion]Optimization of culture conditions and process can significantly increase the amount of marine red yeast and carotenoid content.
引文
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[2]李臣,阮榕生,林向阳,等.红酵母的性质及其应用研究[J].农产品加工,2006(5):20-22,25.
[3]刘秀莲,王宇光.海洋红酵母的研究进展[J].生物技术通讯,2008,19(2):293-295.
[4]赵昌会,罗静瑶.海洋酵母菌的研究进展[J].湖南科技学院学报,2008,29(8):59-60.
[5]张超,刘军.SCP生产菌海洋红酵母摇瓶培养研究[J].四川轻化工学院学报,2010,13(3):71-74.
[6]王宇光,雷禄旺,刘秀莲,等.海南海域海洋红酵母的分离鉴定和应用性能评价[J].中国农学通报,2009,25(22):324-328.
[7]周德庆.微生物实验手册[M].北京:高等教育出版社,2006.
[8]巴尼特JA,佩恩RW,亚罗D.酵母菌的特征与鉴定手册[M].青岛:青岛海洋大学出版社,1991.
[9]徐海,钱卫,高东,等.红酵母产生类胡萝卜素的研究[J].工业微生物,1997,27(3):24-26,31.
[10]张志军,江晓路,张婷婷,等.海洋红酵母Rhodotorula sp.06产类胡萝卜素的研究[J].食品工业科技,2007,28(11):123-125,131.
[11]仇少君.有机无机氮源配合施用对土壤微生物生物量氮及氮素循环的影响[D].长沙:湖南农业大学,2006.
[12]杨莺莺,陈永青,杨铿,等.海洋红酵母RH1菌株发酵培养条件的研究[J].南方水产科学,2012,8(1):67-74.
[13]张卉,李健,李波,等.高产类胡萝卜素酵母菌株LRY-01发酵条件的优化研究[J].工业微生物,2006,36(1):47-50.
[14]孙建男,谢为天,刘颖,等.海洋红酵母的研究进展[J].安徽农业科学,2015,43(4):84-88.