不同油气储藏区微生物代谢活性及多样性响应
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摘要
【目的】研究土壤微生物群落代谢活性及多样性对地下油气储藏情况的响应。【方法】采用Biolog-ECO微平板技术,研究焉耆境内不同油气储藏地区,地下80～100 cm土壤样品微生物的群落代谢活性及多样性。【结果】微生物代谢活性总体呈现利于藏油气区域微生物活性>不利于或难于评价藏油气区域>无藏油气区域的规律;在利于藏油气和不利于或难评价藏油气区域样品中,氨基酸和多聚物利用微生物在AWCD的贡献上均高于对照区域,而羧酸、胺类均低于对照区域。主成分分析表明,三个不同藏油气区域微生物群落分布特征有着明显差异,PCA 1、PCA 2中权重最大的基质为D-甘露醇和D-木糖,分别为载荷值0.97和0.87。【结论】新疆焉耆三个区域,地下土壤微生物群落明显受油气分布影响。Biolog-ECO微平板技术可能作为油气储藏初步勘测的一种手段。
【Objective】 To study the response of soil microbial community metabolic activity and diversity to underground oil and gas storage.【Method】The metabolic activity and diversity of microbial community in 80-100 cm soil samples from different oil and gas storage areas in Yanqi were studied by using Biolog-ECO microplate technique.【Result】In the three areas of Yanqi, Xinjiang that the study analyzed, soil microbial communities were obviously affected by oil and gas distribution. The general rules of microbial metabolic activity were as follows: the microbial activity of favorable reservoir oil and gas region > unfavorable or difficult to evaluate reservoir oil and gas area > no reservoir oil and gas area; The contribution of amino acid and polymer utilization microbes to AWCD in the samples that were favorable to reservoir oil and gas or difficult to evaluate was higher than that in the control area, while carboxyl and amines were lower than those in the control area. Results of principal component analysis showed that the distribution characteristics of microbial communities in three different reservoirs were obviously different, and the most powerful matrixes was the D-mannitol and D-xylose in PCA 1 and PCA 2, whose respective load values were 0.97 and 0.87.【Conclusion】Biolog-ECO microplate technology can be used as a means for preliminary exploration of oil and gas reservoirs.
【Objective】 To study the response of soil microbial community metabolic activity and diversity to underground oil and gas storage.【Method】The metabolic activity and diversity of microbial community in 80-100 cm soil samples from different oil and gas storage areas in Yanqi were studied by using Biolog-ECO microplate technique.【Result】In the three areas of Yanqi, Xinjiang that the study analyzed, soil microbial communities were obviously affected by oil and gas distribution. The general rules of microbial metabolic activity were as follows: the microbial activity of favorable reservoir oil and gas region > unfavorable or difficult to evaluate reservoir oil and gas area > no reservoir oil and gas area; The contribution of amino acid and polymer utilization microbes to AWCD in the samples that were favorable to reservoir oil and gas or difficult to evaluate was higher than that in the control area, while carboxyl and amines were lower than those in the control area. Results of principal component analysis showed that the distribution characteristics of microbial communities in three different reservoirs were obviously different, and the most powerful matrixes was the D-mannitol and D-xylose in PCA 1 and PCA 2, whose respective load values were 0.97 and 0.87.【Conclusion】Biolog-ECO microplate technology can be used as a means for preliminary exploration of oil and gas reservoirs.
引文
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[4] 张春林,庞雄奇,梅海,等.微生物油气勘探技术的实践与发展[J].新疆石油地质,2010,31(3):320-322.ZHANG Chun-lin, PANG Xiong-qi, MEI Hai, et al. (2010)Application and Progress of Microbial Oil Survey Technique(MOST)[J]. Xinjiang Petroleum Geology, 31(3):320-322. (in Chinese)
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[6] 颜承志,施和生,庞雄,等.微生物地球化学勘探技术在白云凹陷深水区油气勘探中的应用[J].中国海上油气,2014,26(4):15-19.YAN Cheng-zhi, SHI He-sheng, PANG Xiong, et al. (2014). Applying a technique of microbiological geochemistry to hydrocarbon exploration in Baiyun sag deep water area [J]. China Offshore Oil and Gas, 26(4):15-19. (in Chinese)
[7] 林社卿,邱荣华,李永林,等.焉耆盆地侏罗系油气成藏期次分析[J].地球科学, 2003,(1): 71-77.LIN She-qing, QIN Rong-hua, LI Yong-lin, et al. (2003). Analysis of Pool Forming Periods in Jurassic Yanqi Basin [J]. Journal of Earth Science, (1): 71-77.(in Chinese)
[8] 郭召杰,张志诚,钱详麟,等.新疆焉耆盆地构造特征及其石油地质意义[J].石油实验地质, 1998,20(3): 205-209.GUO Zhao jie, ZHANG Zhi-cheng, QIAN Xiang-lin, et al. (1998). The structural styles and their role in hydrocarbon accumulation of the Yanqi basin, Northwest China [J]. Petroleum Geology & Experiment, 20(3): 205-209. (in Chinese)
[9] 柳广弟,张仲培,陈文学,等.焉耆盆地侏罗系油气系统特征与演化[J].石油学报,2002,(6):69-71.LIU Guang-di, ZHANG Zhong-pei, CHEN Wen-xue, et al. (2002). Study on the period and time of oil and gas accumulation in Yanqi basin, Northwest China [J]. Petroleum Exploration and Development, (6):69-71. (in Chinese)
[10] Hubert, C., & Judd, A. (2010). Using Microorganisms as Prospecting Agents in Oil and Gas Exploration. Springer Berlin Heidelberg.
[11] Yuan, Z. H., Zhang, Y. Q., Zhao, Q., Jiang, H. F., Liu, Y. Y., & Luo, Z. H., et al. (2009). New progress of microbial prospecting of oil and gas in china:taking the satellite oilfield in daqing as an example. Science in China, 52(1): 152-158.
[12] Cadilloquiroz, H., Yashiro, E., Yavitt, J. B., & Zinder, S. H. (2008). Characterization of the archaeal community in a minerotrophic fen and terminal restriction fragment length polymorphism-directed isolation of a novel hydrogenotrophic methanogen. Applied & Environmental Microbiology, 74(7): 2,059-2,068.
[13] Alamshahi, S., & Hataf, N. (2009). Bearing capacity of strip footings on sand slopes reinforced with geogrid and grid-anchor. Geotextiles & Geomembranes, 27(3): 217-226.
[14] Kong, X., Wang, C., & Ji, M. (2013). Analysis of microbial metabolic characteristics in mesophilic and thermophilic biofilters using biolog plate technique. Chemical Engineering Journal, 230(16): 415-421.
[15] Selmants, P. C., Hart, S. C., Boyle, S. I., & Stark, J. M. (2005). Red Alder (Almus rubra) Alters Community-Level Soil Microbial Function in Conifer Forests of the Pacific Northwest, USA. Soil Biology & Biochemistry, 37(10): 1,860-1,868.
[16] 王旭辉,晁群芳,徐鑫,等.石油污染土壤的生物修复室内模拟实验研究[J].环境工程学报,2012,6(5):1 663-1 668.WANG Xu-hui, CHAO Qun-fang, XU Xin, et al. (2012). Experimental study on bioremediation of oil-contaminated soil [J]. Chinese Journal of Environmental Engineering, 6(5):1,663-1,668.(in Chinese)
[17] 赵亚光,段魏魏,晁群芳,等.克拉玛依石油污染土壤微生物类群及其代谢活性研究[J].环境科学与技术,2017,40(4):7-12.ZHAO Ya-guang, DUAN Wei-wei, CHAO Qun-fang,et al. (2017). Microbial Groups and Metabolic Activation of Oil-contaminated Soil in Karamay [J]. Environmental Science & Technology,40(4):7-12.(in Chinese)
[18] 宋收,陈晓明,肖伟,等.基于BIOLOG指纹解析土壤可培微生物对铀污染的响应[J].核农学报,2016,30(6):1 169-1 177.SONG Shou, CHEN Xiao-min, XIAO Wei, et al. (2016). Response of Soil Cultivatable Microorganisms to Uranium Contamination Based on BIOLOG Fingerprint Analysis Method [J]. Journal of Nuclear Agricultural Sciences,30(6):1,169-1,177.(in Chinese)
[19] 杨帆,沈忠民,汤玉平,等.准噶尔盆地春光探区油气微生物指示[J].石油学报,2017,38(7):804-812.YANG Fan, SHEN Zhong-min, TANG Yu-ping, et al. (2017). Hydrocarbon microbial prospecting in Chunguang exploration area, Jungar Basin [J]. Acta Petrolei Sinica, 38(7):804-812.(in Chinese)
[20] 赵东风,吴伟林,张云波等.克拉玛依石油污染土壤生物修复的初步研究[J].石油学报(石油加工),2012,28(4):696-704.ZHAO Dong-feng, WU Wei-ling, ZHANG Yun-bo, et al. (2012). Primary study on the Bio-remediation of oil contaminated soil of Karamay oil field [J]. Acta Petrolei Sinica (Petroleum Processing Section) , 28(4):696-704.(in Chinese)
[21] 李春荣,王文科,曹玉清.石油污染土壤的生物修复研究[J].农业环境科学学报,2009, 28(2):234-238.LI Chun-rong, WANG Wen-ke, CAO Yu-qing. (2009). Bioremediation of Petroleum-contaminated Soil [J]. Journal of Agro-Environment Science, 28(2):234-238.(in Chinese)
[22] 丁力, 吴宇兵, 刘芬芬.中拐凸起火山岩油气藏微生物地球化学勘探研究[J].特种油气藏, 2018,(4):1-6.DING Li, WU Yu-bing, LIU Fen-fen. (2018). Microbial geochemical exploration of volcanic reservoirs in the central Guai uplift [J]. Special Oil & Gas Reservoirs, (4):1-6.(in Chinese)
[23] 廖先燕, 张涛, 曾军,等.一种基于BIOLOG代谢指纹分析的油气微生物勘探新方法[J].微生物学报, 2012, 52(4):505-511.LIAO Xian-yan, ZHANG Tao, ZENG Jun, et al. (2012).A new method of microbial prospecting for oil and gas based on BIOLOG metabolic fingerprinting analysis [J]. Acta Microbiologica Sinica, 52(4):505-511. (in Chinese)
[2] 黄希.新疆石油天然气产业的发展现状研究[J].企业导报,2010,(3):101-111. HUANG Xi. (2010). Research on the development status of Xinjiang oil and natural gas industry [J]. Guide to Business, (3):101-111. (In Chinese)
[3] Moqilewskii G A. (1938). Microbiological investigation inconnecting with gas Surveying. Razvedka Nedr, (8):59-68.
[4] 张春林,庞雄奇,梅海,等.微生物油气勘探技术的实践与发展[J].新疆石油地质,2010,31(3):320-322.ZHANG Chun-lin, PANG Xiong-qi, MEI Hai, et al. (2010)Application and Progress of Microbial Oil Survey Technique(MOST)[J]. Xinjiang Petroleum Geology, 31(3):320-322. (in Chinese)
[5] 梅海,林壬子,梅博文,等.油气微生物检测技术:理论、实践和应用前景[J].天然气地球科学,2008,19(6):888-893. MEI Hai, LIN Ren-zi, MEI Bo-wen, et al. (2008). Microbial Oil-gas Detection Technologies: Theory, Practice and Application Prospect [J]. Natural Gas Geoscience, 19(6): 888-893. (in Chinese)
[6] 颜承志,施和生,庞雄,等.微生物地球化学勘探技术在白云凹陷深水区油气勘探中的应用[J].中国海上油气,2014,26(4):15-19.YAN Cheng-zhi, SHI He-sheng, PANG Xiong, et al. (2014). Applying a technique of microbiological geochemistry to hydrocarbon exploration in Baiyun sag deep water area [J]. China Offshore Oil and Gas, 26(4):15-19. (in Chinese)
[7] 林社卿,邱荣华,李永林,等.焉耆盆地侏罗系油气成藏期次分析[J].地球科学, 2003,(1): 71-77.LIN She-qing, QIN Rong-hua, LI Yong-lin, et al. (2003). Analysis of Pool Forming Periods in Jurassic Yanqi Basin [J]. Journal of Earth Science, (1): 71-77.(in Chinese)
[8] 郭召杰,张志诚,钱详麟,等.新疆焉耆盆地构造特征及其石油地质意义[J].石油实验地质, 1998,20(3): 205-209.GUO Zhao jie, ZHANG Zhi-cheng, QIAN Xiang-lin, et al. (1998). The structural styles and their role in hydrocarbon accumulation of the Yanqi basin, Northwest China [J]. Petroleum Geology & Experiment, 20(3): 205-209. (in Chinese)
[9] 柳广弟,张仲培,陈文学,等.焉耆盆地侏罗系油气系统特征与演化[J].石油学报,2002,(6):69-71.LIU Guang-di, ZHANG Zhong-pei, CHEN Wen-xue, et al. (2002). Study on the period and time of oil and gas accumulation in Yanqi basin, Northwest China [J]. Petroleum Exploration and Development, (6):69-71. (in Chinese)
[10] Hubert, C., & Judd, A. (2010). Using Microorganisms as Prospecting Agents in Oil and Gas Exploration. Springer Berlin Heidelberg.
[11] Yuan, Z. H., Zhang, Y. Q., Zhao, Q., Jiang, H. F., Liu, Y. Y., & Luo, Z. H., et al. (2009). New progress of microbial prospecting of oil and gas in china:taking the satellite oilfield in daqing as an example. Science in China, 52(1): 152-158.
[12] Cadilloquiroz, H., Yashiro, E., Yavitt, J. B., & Zinder, S. H. (2008). Characterization of the archaeal community in a minerotrophic fen and terminal restriction fragment length polymorphism-directed isolation of a novel hydrogenotrophic methanogen. Applied & Environmental Microbiology, 74(7): 2,059-2,068.
[13] Alamshahi, S., & Hataf, N. (2009). Bearing capacity of strip footings on sand slopes reinforced with geogrid and grid-anchor. Geotextiles & Geomembranes, 27(3): 217-226.
[14] Kong, X., Wang, C., & Ji, M. (2013). Analysis of microbial metabolic characteristics in mesophilic and thermophilic biofilters using biolog plate technique. Chemical Engineering Journal, 230(16): 415-421.
[15] Selmants, P. C., Hart, S. C., Boyle, S. I., & Stark, J. M. (2005). Red Alder (Almus rubra) Alters Community-Level Soil Microbial Function in Conifer Forests of the Pacific Northwest, USA. Soil Biology & Biochemistry, 37(10): 1,860-1,868.
[16] 王旭辉,晁群芳,徐鑫,等.石油污染土壤的生物修复室内模拟实验研究[J].环境工程学报,2012,6(5):1 663-1 668.WANG Xu-hui, CHAO Qun-fang, XU Xin, et al. (2012). Experimental study on bioremediation of oil-contaminated soil [J]. Chinese Journal of Environmental Engineering, 6(5):1,663-1,668.(in Chinese)
[17] 赵亚光,段魏魏,晁群芳,等.克拉玛依石油污染土壤微生物类群及其代谢活性研究[J].环境科学与技术,2017,40(4):7-12.ZHAO Ya-guang, DUAN Wei-wei, CHAO Qun-fang,et al. (2017). Microbial Groups and Metabolic Activation of Oil-contaminated Soil in Karamay [J]. Environmental Science & Technology,40(4):7-12.(in Chinese)
[18] 宋收,陈晓明,肖伟,等.基于BIOLOG指纹解析土壤可培微生物对铀污染的响应[J].核农学报,2016,30(6):1 169-1 177.SONG Shou, CHEN Xiao-min, XIAO Wei, et al. (2016). Response of Soil Cultivatable Microorganisms to Uranium Contamination Based on BIOLOG Fingerprint Analysis Method [J]. Journal of Nuclear Agricultural Sciences,30(6):1,169-1,177.(in Chinese)
[19] 杨帆,沈忠民,汤玉平,等.准噶尔盆地春光探区油气微生物指示[J].石油学报,2017,38(7):804-812.YANG Fan, SHEN Zhong-min, TANG Yu-ping, et al. (2017). Hydrocarbon microbial prospecting in Chunguang exploration area, Jungar Basin [J]. Acta Petrolei Sinica, 38(7):804-812.(in Chinese)
[20] 赵东风,吴伟林,张云波等.克拉玛依石油污染土壤生物修复的初步研究[J].石油学报(石油加工),2012,28(4):696-704.ZHAO Dong-feng, WU Wei-ling, ZHANG Yun-bo, et al. (2012). Primary study on the Bio-remediation of oil contaminated soil of Karamay oil field [J]. Acta Petrolei Sinica (Petroleum Processing Section) , 28(4):696-704.(in Chinese)
[21] 李春荣,王文科,曹玉清.石油污染土壤的生物修复研究[J].农业环境科学学报,2009, 28(2):234-238.LI Chun-rong, WANG Wen-ke, CAO Yu-qing. (2009). Bioremediation of Petroleum-contaminated Soil [J]. Journal of Agro-Environment Science, 28(2):234-238.(in Chinese)
[22] 丁力, 吴宇兵, 刘芬芬.中拐凸起火山岩油气藏微生物地球化学勘探研究[J].特种油气藏, 2018,(4):1-6.DING Li, WU Yu-bing, LIU Fen-fen. (2018). Microbial geochemical exploration of volcanic reservoirs in the central Guai uplift [J]. Special Oil & Gas Reservoirs, (4):1-6.(in Chinese)
[23] 廖先燕, 张涛, 曾军,等.一种基于BIOLOG代谢指纹分析的油气微生物勘探新方法[J].微生物学报, 2012, 52(4):505-511.LIAO Xian-yan, ZHANG Tao, ZENG Jun, et al. (2012).A new method of microbial prospecting for oil and gas based on BIOLOG metabolic fingerprinting analysis [J]. Acta Microbiologica Sinica, 52(4):505-511. (in Chinese)