果汁泥中苯甲酸含量的不确定度评定
|
摘要
目的评定高效液相色谱法(high performance liquid chromatography,HPLC)测定果汁泥中苯甲酸的不确定度。方法根据GB 5009.28-2016《食品安全国家标准食品中苯甲酸、山梨酸和糖精钠的测定》和JJF1059.1-2012《测量不确定度评定与表示》,建立分析HPLC测定果汁泥中苯甲酸的数学模型,通过对整个测定过程中的不确定度来源进行分析和合成,运用最小二乘法对苯甲酸标准曲线拟合的不确定度进行评定。结果在95%置信区间下, k=2,当取样量为2.0630 g,测得果汁泥中苯甲酸含量为(5.997±0.746)μg/kg。结论该实验的主要不确定度来源为标准溶液配制、样品稀释以及标准曲线拟合。
Objective To evaluate the uncertainty of determination of benzoic acid content in juice mud by high performance liquid chromatography(HPLC). Methods According to GB 5009.28-2016 National food safety standards-Determination of benzoic acid, sorbic acid and saccharin sodium in food and JJF 1059.1-2012 Measurement and expression of measurement uncertainty, the mathematical model was established for the determination of the uncertainty of benzoic acid content in juice mud by HPLC. The least squares method was used to assess the uncertainty of the fitting of the standard curve by analyzing and synthesizing the sources of uncertainty throughout the assay. Results When the sample was 2.0630 g, the result of determination of the residual amount of benzoic acid content in juice mud was(5.997±0.746) μg/kg at the 95% confidence interval(k=2). Conclusion The main sources of uncertainty are standard solution preparation, sample dilution and standard curve fitting.
Objective To evaluate the uncertainty of determination of benzoic acid content in juice mud by high performance liquid chromatography(HPLC). Methods According to GB 5009.28-2016 National food safety standards-Determination of benzoic acid, sorbic acid and saccharin sodium in food and JJF 1059.1-2012 Measurement and expression of measurement uncertainty, the mathematical model was established for the determination of the uncertainty of benzoic acid content in juice mud by HPLC. The least squares method was used to assess the uncertainty of the fitting of the standard curve by analyzing and synthesizing the sources of uncertainty throughout the assay. Results When the sample was 2.0630 g, the result of determination of the residual amount of benzoic acid content in juice mud was(5.997±0.746) μg/kg at the 95% confidence interval(k=2). Conclusion The main sources of uncertainty are standard solution preparation, sample dilution and standard curve fitting.
引文
[1]曾正渝.苯甲酸在食品和药品中的应用[J].中国药业,2007,16(6):64.Zeng ZY.Application of benzoic acid in food and drugs[J].Chin Pharm,2007,16(6):64
[2]吕娜,沈明浩.食品防腐剂苯甲酸钠的蓄积毒性及精子毒性研究[J].毒理学杂志,2011,25(3):241-242.Lv N,Shen MH.Accumulation toxicity and sperm toxicity of sodium benzoate as food preservative[J].J Toxicol,2011,25(3):241-242.
[3]吕娜.食品防腐剂苯甲酸钠的急性毒性及致畸性试验[J].毒理学杂志,2006,20(5):326-327.Lv N.Acute toxicity and teratogenicity test of sodium benzoate as food preservative[J].J Toxicol,2006,20(5):326-327.
[4]胡明铅,王炯坤,蔡继业,等.应用原子力显微镜分析苯甲酸钠生物毒性[J].生物工程学报,2008,24(8):1428-1432.Hu ML,Wang JK,Cai JY,et al.Biotoxicity analysis of sodium benzoate by atomic force microscopy[J].Chin J Biotechnol,2008,24(8):1428-1432.
[5]GB 5009.28-2016食品安全国家标准食品中苯甲酸、山梨酸和糖精钠的测定[S].GB 5009.28-2016 Food safety national standards determination of benzoic acid-Sorbic acid and saccharin sodium[S].
[6]JJF 1059.1-2012测量不确定度评定与表示[S].JJF 1059.1-2012 Measurement uncertainty evaluation and representation[S].
[7]孙婧,刘晓海.高效液相色谱法测定牛奶中甲砜霉素残留量的不确定度评定[J].食品安全质量检测学报,2018,9(19):5228-5234.Sun J,Liu XH.Uncertainty evaluation of determination of thiamphenicol residues in milk by high performance liquid chromatography[J].J Food Saf Qual,2018,9(19):5228-5234.
[8]付登洲,杨雪娇,黄伟.HPLC法测定乳制品中三聚氰胺含量的不确定度评定[J].食品研究与开发,2011,32(2):108-112.Fu DZ,Yang XJ,Huang W.Uncertainty evaluation of determination of melamine in dairy products by high performance liquid chromatography[J].Food Res Dev,2011,32(2):108-112.
[9]JJG 196-2006常用玻璃量器检定规程[S].JJG 196-2006 Verification rules for common glass meters[S]
[10]CNAS-GL 06:2006化学分析中不确定度的评估指南[S].CNAS-GL 06:2006 Guidelines for the evaluation of uncertainty in chemical analysis[S].
[11]物竞数据库乙醇[EB/OL].[2019-01-12].http://www.basechem.org/chemical/1182.Database ethanol[EB/OL].[2019-01-12].http://www.basechem.org/chemical/1182.
[12]物竞数据库水[EB/OL].[2019-01-01].http://www.basechem.org/chemical/12690.Competition database water[EB/OL].[2019-01-01].http://www.basechem.org/chemical/12690.
[13]林华,许蓉蓉,闭秋华,等.高效液相色谱法测定鱼油软胶囊中维生素D3含量的不确定度评定[J].食品安全质量检测学报,2018,9(12):3177-3182.Lin H,Xu RR,Bi QH,et al.Uncertainty evaluation of determination of vitamin D3 in fish oil soft capsules by high performance liquid chromatography[J].J Food Saf Qual,2018,9(12):3177-3182.
[2]吕娜,沈明浩.食品防腐剂苯甲酸钠的蓄积毒性及精子毒性研究[J].毒理学杂志,2011,25(3):241-242.Lv N,Shen MH.Accumulation toxicity and sperm toxicity of sodium benzoate as food preservative[J].J Toxicol,2011,25(3):241-242.
[3]吕娜.食品防腐剂苯甲酸钠的急性毒性及致畸性试验[J].毒理学杂志,2006,20(5):326-327.Lv N.Acute toxicity and teratogenicity test of sodium benzoate as food preservative[J].J Toxicol,2006,20(5):326-327.
[4]胡明铅,王炯坤,蔡继业,等.应用原子力显微镜分析苯甲酸钠生物毒性[J].生物工程学报,2008,24(8):1428-1432.Hu ML,Wang JK,Cai JY,et al.Biotoxicity analysis of sodium benzoate by atomic force microscopy[J].Chin J Biotechnol,2008,24(8):1428-1432.
[5]GB 5009.28-2016食品安全国家标准食品中苯甲酸、山梨酸和糖精钠的测定[S].GB 5009.28-2016 Food safety national standards determination of benzoic acid-Sorbic acid and saccharin sodium[S].
[6]JJF 1059.1-2012测量不确定度评定与表示[S].JJF 1059.1-2012 Measurement uncertainty evaluation and representation[S].
[7]孙婧,刘晓海.高效液相色谱法测定牛奶中甲砜霉素残留量的不确定度评定[J].食品安全质量检测学报,2018,9(19):5228-5234.Sun J,Liu XH.Uncertainty evaluation of determination of thiamphenicol residues in milk by high performance liquid chromatography[J].J Food Saf Qual,2018,9(19):5228-5234.
[8]付登洲,杨雪娇,黄伟.HPLC法测定乳制品中三聚氰胺含量的不确定度评定[J].食品研究与开发,2011,32(2):108-112.Fu DZ,Yang XJ,Huang W.Uncertainty evaluation of determination of melamine in dairy products by high performance liquid chromatography[J].Food Res Dev,2011,32(2):108-112.
[9]JJG 196-2006常用玻璃量器检定规程[S].JJG 196-2006 Verification rules for common glass meters[S]
[10]CNAS-GL 06:2006化学分析中不确定度的评估指南[S].CNAS-GL 06:2006 Guidelines for the evaluation of uncertainty in chemical analysis[S].
[11]物竞数据库乙醇[EB/OL].[2019-01-12].http://www.basechem.org/chemical/1182.Database ethanol[EB/OL].[2019-01-12].http://www.basechem.org/chemical/1182.
[12]物竞数据库水[EB/OL].[2019-01-01].http://www.basechem.org/chemical/12690.Competition database water[EB/OL].[2019-01-01].http://www.basechem.org/chemical/12690.
[13]林华,许蓉蓉,闭秋华,等.高效液相色谱法测定鱼油软胶囊中维生素D3含量的不确定度评定[J].食品安全质量检测学报,2018,9(12):3177-3182.Lin H,Xu RR,Bi QH,et al.Uncertainty evaluation of determination of vitamin D3 in fish oil soft capsules by high performance liquid chromatography[J].J Food Saf Qual,2018,9(12):3177-3182.