石墨炉原子吸收光谱法测定食品中铬
|
摘要
目的研究食品安全风险监测中铬分析的特点,建立有效和准确的石墨炉原子吸收光谱法测定食品中铬的检测方法。方法采用微波消解、湿法消解、高压消解和干灰化法4种前处理方式消解样品,加入磷酸铵、磷酸二氢铵和钯盐等基体改进剂消除样品干扰、优化仪器条件,进行石墨炉原子吸收光谱法测定食品中铬。结果测定铬的最佳基体改进剂为磷酸二氢铵。4种前处理方式的加标回收率差异无统计学意义(F=1. 026,P> 0. 05),精密度差异无统计学意义(F=0. 998,P> 0. 05)。测定铬的定量限为1. 5μg/L,线性范围0～16. 0μg/L,加标回收试验的回收率在90%～104%之间。结论石墨炉原子吸收光谱法准确、可靠、灵敏度和精密度高,可满足食品中铬的风险监测要求。
Objective To study the characteristics of chromium in food safety risk monitoring,and establish an effective and accurate method for the determination of chromium in food by graphite furnace atomic absorption spectrometry. Methods The samples were digested by microwave digestion,wet digestion,high pressure digestion and dry ashing. The matrix modifiers such as ammonium phosphate,ammonium dihydrogen phosphate and palladium salt were added to eliminate sample interference and optimize the instrument conditions,and determine chromium in food by graphite furnace atomic absorption spectrometry. Results The best matrix modifier for the determination of chromium is ammonium dihydrogen phosphate. There was no significant difference in the recovery rate of the four pretreatment methods(F = 1. 026,P > 0. 05). There was no significant difference in precision(F = 0. 998,P > 0. 05). The limit of quantification of chromium was 1. 5 μg/L,the linear range was 0 ～ 16. 0 μg/L,and the recovery of the spiked recovery test was between 90% and 104%. Conclusion Graphite furnace atomic absorption spectrometry is accurate,reliable,sensitive and precise,and can meet the risk monitoring requirements of chromium in food.
Objective To study the characteristics of chromium in food safety risk monitoring,and establish an effective and accurate method for the determination of chromium in food by graphite furnace atomic absorption spectrometry. Methods The samples were digested by microwave digestion,wet digestion,high pressure digestion and dry ashing. The matrix modifiers such as ammonium phosphate,ammonium dihydrogen phosphate and palladium salt were added to eliminate sample interference and optimize the instrument conditions,and determine chromium in food by graphite furnace atomic absorption spectrometry. Results The best matrix modifier for the determination of chromium is ammonium dihydrogen phosphate. There was no significant difference in the recovery rate of the four pretreatment methods(F = 1. 026,P > 0. 05). There was no significant difference in precision(F = 0. 998,P > 0. 05). The limit of quantification of chromium was 1. 5 μg/L,the linear range was 0 ～ 16. 0 μg/L,and the recovery of the spiked recovery test was between 90% and 104%. Conclusion Graphite furnace atomic absorption spectrometry is accurate,reliable,sensitive and precise,and can meet the risk monitoring requirements of chromium in food.
引文
[1] REILY C.食品的金属污染[M].北京:轻工业出版社,1986:90-130.
[2]中华人民共和国卫生部. GB 2762-2017食品安全国家标准食品中污染物限量标准[S].北京:中国标准出版社,2013.
[3]胡曙光,梁春穗,鲁林,等.石墨炉原子吸收法测定食品安全风险监测中痕量铝的测定[J].中国卫生检验杂志,2012,22(3):453-455.
[4]中华人民共和国卫生和计划生育委员会. GB 5009. 123-2014食品安全国家标准食品中铬的测定[S].北京:中国标准出版社,2014.
[5]刘献新,李清昌.石墨炉原子吸收分析中的基体改进技术及应用[J].分析试验室,2008,27(S1):477-480.
[6]陈召桂,成凌,朱灵珠,等.微波消解-原子吸收法测不同食品中铬的含量[J].食品工程,2015,43(1):59-61.
[7]王晓波,李建国,赵春香,等.广州市售食品总铬和六价铬的含量分析[J].食品研究与开发,2014,36(22):86-89.
[8]赵嵩,陈云,吴妙姗.乳浊液直接进样石墨炉原子吸收光谱法测定牛奶中的铬[J].理化检验(化学分册),2014,50(12):1590-1591.
[9]王竹天.食品卫生检验方法(理化部分)注解(上)[M].北京:中国标准出版社,2008.
[10]陈彬,杨蕾,何广新,等.微波消解-电感耦合等离子体质谱法测定保健食品中铬、砷、镉、铅和汞[J].中国卫生检验杂志,2013,23(15):3043-3046.
[11]杨定清,周娅,雷绍荣,等.攀枝花市芒果基地土壤和芒果镉污染调查研究[J].农业环境科学学报,2010,30(S1):104-106.
[12]王瑞芬,高筱玲.食品中铬测定的前处理技术研究[J].化工管理,2013,26(24):111.
[13]彭靖茹,甘志勇.在线标准加入-石墨炉原子吸收光谱法测定食品中铬和镉[J].理化检验(化学分册),2009,45(8):903-904.
[2]中华人民共和国卫生部. GB 2762-2017食品安全国家标准食品中污染物限量标准[S].北京:中国标准出版社,2013.
[3]胡曙光,梁春穗,鲁林,等.石墨炉原子吸收法测定食品安全风险监测中痕量铝的测定[J].中国卫生检验杂志,2012,22(3):453-455.
[4]中华人民共和国卫生和计划生育委员会. GB 5009. 123-2014食品安全国家标准食品中铬的测定[S].北京:中国标准出版社,2014.
[5]刘献新,李清昌.石墨炉原子吸收分析中的基体改进技术及应用[J].分析试验室,2008,27(S1):477-480.
[6]陈召桂,成凌,朱灵珠,等.微波消解-原子吸收法测不同食品中铬的含量[J].食品工程,2015,43(1):59-61.
[7]王晓波,李建国,赵春香,等.广州市售食品总铬和六价铬的含量分析[J].食品研究与开发,2014,36(22):86-89.
[8]赵嵩,陈云,吴妙姗.乳浊液直接进样石墨炉原子吸收光谱法测定牛奶中的铬[J].理化检验(化学分册),2014,50(12):1590-1591.
[9]王竹天.食品卫生检验方法(理化部分)注解(上)[M].北京:中国标准出版社,2008.
[10]陈彬,杨蕾,何广新,等.微波消解-电感耦合等离子体质谱法测定保健食品中铬、砷、镉、铅和汞[J].中国卫生检验杂志,2013,23(15):3043-3046.
[11]杨定清,周娅,雷绍荣,等.攀枝花市芒果基地土壤和芒果镉污染调查研究[J].农业环境科学学报,2010,30(S1):104-106.
[12]王瑞芬,高筱玲.食品中铬测定的前处理技术研究[J].化工管理,2013,26(24):111.
[13]彭靖茹,甘志勇.在线标准加入-石墨炉原子吸收光谱法测定食品中铬和镉[J].理化检验(化学分册),2009,45(8):903-904.