贵州地方粳稻‘黎平杂边禾’矮秆小粒突变体dss1鉴定与基因定位
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摘要
本研究从贵州地方粳稻(Oryza sativa ssp.japonica)品种‘黎平杂边禾’甲基磺酸乙酯(EMS)突变体库中筛选获得一份能稳定遗传的矮秆小粒突变体,暂命名为dss1(dwarf and small seed 1)。与野生型相比,dss1表现为植株矮化、株型直立、叶色深绿、籽粒变小、第二节间严重缩短、穗长增加等典型油菜素内酯(BR)缺陷突变体的性状。显微观察结果表明dss1叶鞘表皮细胞的长度变短,可能是突变体第二叶鞘长度比野生型短的原因。对突变体的暗形态建成与BR敏感性研究表明,黑暗条件下突变体表现为去黄化表型,对外源BR敏感。遗传特性分析证明dss1突变体由一个隐性单基因位点控制。利用Mut Map技术将dss1基因定位于3号染色体上,筛选获得一个候选基因,测序结果表明,dss1候选基因为BR合成途径关键酶基因Os DWARF,dss1是由于该基因第5个外显子上第335位的氨基酸由苏氨酸(ACT)突变为异亮氨酸(ATT)所引起的。定位得到的矮化小粒基因DSS1为一个新的Os DWARF等位基因。
A stably inherited dwarf and small seed mutant dss1 was identified from ethyl methane sulfonate(EMS) mutant progeny of ‘Lipingzabianhe', a rice(Oryza sativa ssp. japonica) landrace in Guizhou. Compared to wild type(WT), dss1 mutant showed the typical brassinosteroid(BR)-deficient phenotype, with erect and dark green leaves, small seeds, un-elongated second internodes and longer panicles. The leaf sheath epidermal cells were shorter than those of the wild types. To determine whether the dwarf phenotype of mutant plants was caused by BR deficiency or insensitivity, we analyzed the response of dss1 to exogenous BR treatment and skotomorphogenesis. The results suggest that the dss1 plant showed a deetiolated phenotype in the darkness, and was sensitive to exogenous BR. Genetic analysis and F2 progeny segregation results reveal dss1 was controlled by a pair of recessive genes. By employing the Mut Map method, we revealed that dss1 contains a mutation in the Os DWARF gene on chromosome 3. The dss1 mutation occured in the 5 th exon of Os DWARF, which caused a nonsense mutation of a threonine(ACT) codon change to an isoleucine(ATT) at residue 335. We confirmed that DSS1 mapped in this study is a new allelic gene of Os DWARF.
A stably inherited dwarf and small seed mutant dss1 was identified from ethyl methane sulfonate(EMS) mutant progeny of ‘Lipingzabianhe', a rice(Oryza sativa ssp. japonica) landrace in Guizhou. Compared to wild type(WT), dss1 mutant showed the typical brassinosteroid(BR)-deficient phenotype, with erect and dark green leaves, small seeds, un-elongated second internodes and longer panicles. The leaf sheath epidermal cells were shorter than those of the wild types. To determine whether the dwarf phenotype of mutant plants was caused by BR deficiency or insensitivity, we analyzed the response of dss1 to exogenous BR treatment and skotomorphogenesis. The results suggest that the dss1 plant showed a deetiolated phenotype in the darkness, and was sensitive to exogenous BR. Genetic analysis and F2 progeny segregation results reveal dss1 was controlled by a pair of recessive genes. By employing the Mut Map method, we revealed that dss1 contains a mutation in the Os DWARF gene on chromosome 3. The dss1 mutation occured in the 5 th exon of Os DWARF, which caused a nonsense mutation of a threonine(ACT) codon change to an isoleucine(ATT) at residue 335. We confirmed that DSS1 mapped in this study is a new allelic gene of Os DWARF.
引文
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Arite T,Iwata H,Ohshima K,et al(2007).DWARF10,an RMS1/MAX4/DAD1 ortholog,controls lateral bud outgrowth in rice.Plant J,51(6):1019-1029
Chen X,Zeng X,Zhao D(2016).The photosynthetic characteristics and protect enzyme activity of a small grain and dwarf mutant dss-1 of Lipingzabianhei Guizhou local rice cultivar.Mol Plant Breed,14(6):1602-1608(in Chinese with English abstract)[陈锡,曾晓芳,赵德刚(2016).贵州黎平杂边禾突变体dss-1光合生理特性与保护酶活性研究.分子植物育种,14(6):1602-1608]
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Guo X,Hou X,Fang J,et al(2013).The rice GERMINATIONDEFECTIVE 1,encoding a B3 domain transcriptional repressor,regulates seed germination and seedling development by integrating GA and carbohydrate metabolism.Plant J,75(3):403-416
Hong Z,Ueguchi-Tanaka M,Fujioka S,et al(2005).The Rice brassinosteroid-deficient dwarf2 mutant,defective in the rice homolog of Arabidopsis DIMINUTO/DWARF1,is rescued by the endogenously accumulated alternative bioactive brassinosteroid,dolichosterone.Plant Cell,17(8):2243-2254
Hong Z,Ueguchi-Tanaka M,Shimizu-Sato S,et al(2002).Loss-of-function of a rice brassinosteroid biosynthetic enzyme,C-6 oxidase,prevents the organized arrangement and polar elongation of cells in the leaves and stem.Plant J,32(4):495-508
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Jiang Y,Bao L,Jeong SY,et al(2012).XIAO is involved in the control of organ size by contributing to the regulation of signaling and homeostasis of brassinosteroids and cell cycling in rice.Plant J,70(3):398-408
Kitagawa K,Kurinami S,Oki K,et al(2010).A novel kinesin13 protein regulating rice seed length.Plant Cell Physiol,51(8):1315-1329
Komorisono M,Ueguchi-Tanaka M,Aichi I,et al(2005).Analysis of the rice mutant dwarf and gladius leaf 1.Aberrant katanin-mediated microtubule organization causes up-regulation of gibberellin biosynthetic genes independently of gibberellin signaling.Plant Physiol,138(4):1982-1993
Li H,Zuo QY,Tu SB(2015).Advances in brassinosteroid biosynthesis and metabolism.Plant Physiol J,51(11):1787-1798(in Chinese with English abstract)[李辉,左钦月,涂升斌(2015).油菜素内酯生物合成和代谢研究进展.植物生理学报,51(11):1787-1798]
Lin Z,Li X,Shannon LM,et al(2012).Parallel domestication of the Shattering1 genes in cereals.Nat Genet,44(6):720-724
Liu X,Feng ZM,Zhou CL,et al(2016).Brassinosteroid(BR)biosynthetic gene lhdd10 controls late heading and plant height in rice(Oryza sativa L.).Plant Cell Rep,35(2):357-368
Mori M,Nomura T,Ooka H,et al(2002).Isolation and characterization of a rice dwarf mutant with a defect in brassinosteroid biosynthesis.Plant Physiol,130(3):1152-1161
Nakamura A,Fujioka S,Sunohara H,et al(2006).The role of Os BRI1 and its homologous genes,Os BRL1 and Os BRL3,in rice.Plant Physiol,140(2):580-590
Nguyen VNT,Moon S,Jung KH(2014).Genome-wide expression analysis of rice ABC transporter family across spatio-temporal samples and in response to abiotic stresses.J Plant Physiol,171(14):1276-1288
Qian Q,Guo L,Smith SM,et al(2016).Breeding high-yield superior quality hybrid super rice by rational design.Natl Sci Rev,3:283-294
Sakamoto T,Miura K,Itoh H,et al(2004).An overview of gibberellin metabolism enzyme genes and their related mutants in rice.Plant Physiol,134(4):1642-1653
Saleh A,Lumbreras V,Lopez C,et al(2006).Maize DBF1-interactor protein 1 containing an R3H domain is a potential regulator of DBF1 activity in stress responses.Plant J,46(5):747-757
Shi Z,Rao Y,Xu J,et al(2015).Characterization and cloning of SMALL GRAIN 4,a novel DWARF11 allele that affects brassinosteroid biosynthesis in rice.Sci Bull,60(10):905-915
Sun C,Li J(2017).Biosynthesis,catabolism,and signal transduction of brassinosteroids.Plant Physiol J,53(3):291-307(in Chinese with English abstract)[孙超,黎家(2017).油菜素甾醇类激素的生物合成、代谢及信号转导.植物生理学报,53(3):291-307]
Tanabe S,Ashikari M,Fujioka S,et al(2005).A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant,dwarf11,with reduced seed length.Plant Cell,17(3):776-790
Tong H,Liu L,Jin Y,et al(2012).DWARF AND LOW-TIL-LERING acts as a direct downstream target of a GSK3/SHAGGY-like kinase to mediate brassinosteroid responses in rice.Plant Cell,24(6):2562-2577
Wang N,Long T,Yao W,et al(2013).Mutant resources for the functional analysis of the rice genome.Mol Plant,6(3):596-604
Wang Y,Li J(2008).Molecular basis of plant architecture.Annu Rev Plant Biol,59:253-279
Wang Y,Li J(2011).Branching in rice.Curr Opin Plant Biol,14:94-99
Wei Z,Li J(2016).Brassinosteroids regulate root growth,development,and symbiosis.Mol Plant,9:86-100
Wu Y,Fu Y,Zhao S,et al(2016).CLUSTERED PRIMARYBRANCH 1,a new allele of DWARF11,controls panicle architecture and seed size in rice.Plant Biotechnol J,14:377-386
Xiao JF,Wu CY,Yuan M,et al(2015).The progress and perspective of rice functional genomics research in China.Chin Sci Bull,60(18):1711-1723(in Chinese with English abstract)[肖景华,吴昌银,袁猛等(2015).中国水稻功能基因组研究进展与展望.科学通报,60(18):1711-1723]
Xie X,Yoneyama K,Yoneyama K(2010).The strigolactone story.Annu Rev Phytopathol,48:93-117
Xue W,Xing Y,Weng X,et al(2008).Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice.Nat Genet,40(6):761-767
Yamamuro C,Ihara Y,Wu X,et al(2000).Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint.Plant Cell,12(9):1591-1605
Yan WH,Wang P,Chen HX,et al(2011).A major QTL,Ghd8,plays pleiotropic roles in regulating grain productivity,plant height,and heading date in rice.Mol Plant,4(2):319-330
Yang XC,Hwa CM(2008).Genetic modification of plant architecture and variety improvement in rice.Heredity,101(5):396-404
Zeng X,Liao X,Zhao D(2014).Identification and phenotypic expression of a semi-dwarf mutant bgsd-2 of the Guizhou local black glutinous rice cultivar.Guizhou Agr Sci,42(5):1-6(in Chinese with English abstract)[曾晓芳,廖宣峰,赵德刚(2014).贵州黑糯稻半矮秆突变体bgsd-2的鉴选及性状表现.贵州农业科学,42(5):1-6]
Arite T,Iwata H,Ohshima K,et al(2007).DWARF10,an RMS1/MAX4/DAD1 ortholog,controls lateral bud outgrowth in rice.Plant J,51(6):1019-1029
Chen X,Zeng X,Zhao D(2016).The photosynthetic characteristics and protect enzyme activity of a small grain and dwarf mutant dss-1 of Lipingzabianhei Guizhou local rice cultivar.Mol Plant Breed,14(6):1602-1608(in Chinese with English abstract)[陈锡,曾晓芳,赵德刚(2016).贵州黎平杂边禾突变体dss-1光合生理特性与保护酶活性研究.分子植物育种,14(6):1602-1608]
Clouse SD(2011).Brassinosteroid signal transduction:from receptor kinase activation to transcriptional networks regulating plant development.Plant Cell,23(4):1219-1230
Guo X,Hou X,Fang J,et al(2013).The rice GERMINATIONDEFECTIVE 1,encoding a B3 domain transcriptional repressor,regulates seed germination and seedling development by integrating GA and carbohydrate metabolism.Plant J,75(3):403-416
Hong Z,Ueguchi-Tanaka M,Fujioka S,et al(2005).The Rice brassinosteroid-deficient dwarf2 mutant,defective in the rice homolog of Arabidopsis DIMINUTO/DWARF1,is rescued by the endogenously accumulated alternative bioactive brassinosteroid,dolichosterone.Plant Cell,17(8):2243-2254
Hong Z,Ueguchi-Tanaka M,Shimizu-Sato S,et al(2002).Loss-of-function of a rice brassinosteroid biosynthetic enzyme,C-6 oxidase,prevents the organized arrangement and polar elongation of cells in the leaves and stem.Plant J,32(4):495-508
Hong Z,Ueguchi-Tanaka M,Umemura K,et al(2003).Arice brassinosteroid-deficient mutant,ebisu dwarf(d2),is caused by a loss of function of a new member of cytochrome P450.Plant Cell,15(12):2900-2910
Jiang Y,Bao L,Jeong SY,et al(2012).XIAO is involved in the control of organ size by contributing to the regulation of signaling and homeostasis of brassinosteroids and cell cycling in rice.Plant J,70(3):398-408
Kitagawa K,Kurinami S,Oki K,et al(2010).A novel kinesin13 protein regulating rice seed length.Plant Cell Physiol,51(8):1315-1329
Komorisono M,Ueguchi-Tanaka M,Aichi I,et al(2005).Analysis of the rice mutant dwarf and gladius leaf 1.Aberrant katanin-mediated microtubule organization causes up-regulation of gibberellin biosynthetic genes independently of gibberellin signaling.Plant Physiol,138(4):1982-1993
Li H,Zuo QY,Tu SB(2015).Advances in brassinosteroid biosynthesis and metabolism.Plant Physiol J,51(11):1787-1798(in Chinese with English abstract)[李辉,左钦月,涂升斌(2015).油菜素内酯生物合成和代谢研究进展.植物生理学报,51(11):1787-1798]
Lin Z,Li X,Shannon LM,et al(2012).Parallel domestication of the Shattering1 genes in cereals.Nat Genet,44(6):720-724
Liu X,Feng ZM,Zhou CL,et al(2016).Brassinosteroid(BR)biosynthetic gene lhdd10 controls late heading and plant height in rice(Oryza sativa L.).Plant Cell Rep,35(2):357-368
Mori M,Nomura T,Ooka H,et al(2002).Isolation and characterization of a rice dwarf mutant with a defect in brassinosteroid biosynthesis.Plant Physiol,130(3):1152-1161
Nakamura A,Fujioka S,Sunohara H,et al(2006).The role of Os BRI1 and its homologous genes,Os BRL1 and Os BRL3,in rice.Plant Physiol,140(2):580-590
Nguyen VNT,Moon S,Jung KH(2014).Genome-wide expression analysis of rice ABC transporter family across spatio-temporal samples and in response to abiotic stresses.J Plant Physiol,171(14):1276-1288
Qian Q,Guo L,Smith SM,et al(2016).Breeding high-yield superior quality hybrid super rice by rational design.Natl Sci Rev,3:283-294
Sakamoto T,Miura K,Itoh H,et al(2004).An overview of gibberellin metabolism enzyme genes and their related mutants in rice.Plant Physiol,134(4):1642-1653
Saleh A,Lumbreras V,Lopez C,et al(2006).Maize DBF1-interactor protein 1 containing an R3H domain is a potential regulator of DBF1 activity in stress responses.Plant J,46(5):747-757
Shi Z,Rao Y,Xu J,et al(2015).Characterization and cloning of SMALL GRAIN 4,a novel DWARF11 allele that affects brassinosteroid biosynthesis in rice.Sci Bull,60(10):905-915
Sun C,Li J(2017).Biosynthesis,catabolism,and signal transduction of brassinosteroids.Plant Physiol J,53(3):291-307(in Chinese with English abstract)[孙超,黎家(2017).油菜素甾醇类激素的生物合成、代谢及信号转导.植物生理学报,53(3):291-307]
Tanabe S,Ashikari M,Fujioka S,et al(2005).A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant,dwarf11,with reduced seed length.Plant Cell,17(3):776-790
Tong H,Liu L,Jin Y,et al(2012).DWARF AND LOW-TIL-LERING acts as a direct downstream target of a GSK3/SHAGGY-like kinase to mediate brassinosteroid responses in rice.Plant Cell,24(6):2562-2577
Wang N,Long T,Yao W,et al(2013).Mutant resources for the functional analysis of the rice genome.Mol Plant,6(3):596-604
Wang Y,Li J(2008).Molecular basis of plant architecture.Annu Rev Plant Biol,59:253-279
Wang Y,Li J(2011).Branching in rice.Curr Opin Plant Biol,14:94-99
Wei Z,Li J(2016).Brassinosteroids regulate root growth,development,and symbiosis.Mol Plant,9:86-100
Wu Y,Fu Y,Zhao S,et al(2016).CLUSTERED PRIMARYBRANCH 1,a new allele of DWARF11,controls panicle architecture and seed size in rice.Plant Biotechnol J,14:377-386
Xiao JF,Wu CY,Yuan M,et al(2015).The progress and perspective of rice functional genomics research in China.Chin Sci Bull,60(18):1711-1723(in Chinese with English abstract)[肖景华,吴昌银,袁猛等(2015).中国水稻功能基因组研究进展与展望.科学通报,60(18):1711-1723]
Xie X,Yoneyama K,Yoneyama K(2010).The strigolactone story.Annu Rev Phytopathol,48:93-117
Xue W,Xing Y,Weng X,et al(2008).Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice.Nat Genet,40(6):761-767
Yamamuro C,Ihara Y,Wu X,et al(2000).Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint.Plant Cell,12(9):1591-1605
Yan WH,Wang P,Chen HX,et al(2011).A major QTL,Ghd8,plays pleiotropic roles in regulating grain productivity,plant height,and heading date in rice.Mol Plant,4(2):319-330
Yang XC,Hwa CM(2008).Genetic modification of plant architecture and variety improvement in rice.Heredity,101(5):396-404
Zeng X,Liao X,Zhao D(2014).Identification and phenotypic expression of a semi-dwarf mutant bgsd-2 of the Guizhou local black glutinous rice cultivar.Guizhou Agr Sci,42(5):1-6(in Chinese with English abstract)[曾晓芳,廖宣峰,赵德刚(2014).贵州黑糯稻半矮秆突变体bgsd-2的鉴选及性状表现.贵州农业科学,42(5):1-6]