油菜素内酯和吲哚乙酸对黄芩生长及品质的影响
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摘要
为黄芩生产和品质改善提供参考,以商洛黄芩幼苗为试验材料,采用盆栽试验研究叶面喷施和浸根2种方式施用不同浓度油菜素内酯(BR)和吲哚乙酸(IAA)植物生长激素对黄芩苗生长及其黄芩苷和黄芩素含量的影响。结果表明:采用叶面喷施和浸根2种方式施用不同浓度BR和IAA溶液对黄芩苗生长及品质的影响明显,并以施用BR和IAA浓度分别为0.1mg/L和50mg/L时的黄芩苗生长及品质最优。其中,叶面喷施黄芩苗的根冠比、株高和侧根数分别为0.75、27.2cm和17条,比叶面喷施清水(CK)黄芩苗分别增加257.14%、32.68%和88.89%;叶面积指数和光合速率分别为8.03和20.58mg/cm2·h,比CK分别提高139.0%和118.24%;黄芩苷和黄芩素含量分别为3.25%和1.05%,比CK分别增加0.66百分点和0.35百分点。浸根黄芩苗的根冠比、株高、侧根数分别为0.78、26.7cm和20条,比清水浸根(CK)黄芩苗分别增加143.75%、31.53%和81.82%;叶面积指数和光合速率分别为7.18和18.65mg/cm2·h,比CK分别提高246.86%和151.69%;黄芩苷和黄芩素含量分别为3.12%和1.03%,比CK分别增加0.55百分点和0.34百分点。采用叶面喷施和浸根2种方式施用适宜浓度BR和IAA均可显著促进黄芩苗的生长、品质改善和药用价值提高,且2种施药方式的黄芩苗生长及品质相当。
Scutellaria baicalensis seedlings were treated with BR and IAA solution with different concentrations by using foliage spray and root dipping patterns to study the effects of BR and IAA on growth,baicalin content and baicalein content of S.baicalensis seedlings and to provide a reference for production and quality improvement of S.baicalensis.Result:The treatment of BR and IAA solution with different concentrations under foliage spray and root dipping patterns has the obvious effect on growth and quality of S.baicalensis seedlings.And growth and quality of S.baicalensis seedlings treated with0.1 mg/L BR and 50 mg/L IAA is the best.The root-shoot ratio,plant height,lateral root number,leaf area index,photosynthetic rate,baicalin content and baicalein content of S.baicalensis seedlings treated with 0.1 mg/L BR and 50 mg/L IAA by the foliage spray pattern are 0.75,27.2 cm,17,8.03,20.58 mg/cm2·h,3.25% and 1.05%,257.14%,32.68%,88.89%,139.0%,118.24%,0.66% and0.35% higher than CK(water foliage spray treatment)respectively.The root-shoot ratio,plant height,lateral root number,leaf area index,photosynthetic rate,baicalin content and baicalein content of S.baicalensis seedlings treated with BR and IAA by the root dipping pattern are 0.78,26.7 cm,20,7.18,18.65 mg/cm2· h,3.12%and 1.03%,143.75%,31.53%,81.82%,246.86%,151.69%,0.55%and0.34% higher than CK(water root dipping treatment)separately.The suitable BR and IAA concentration under foliage spray and root dipping patterns can promote growth of S.baicalensis seedlings and improve quality and medicinal value of S.baicalensis significantly and there are no differences in growth and quality of S.baicalensis between two application patterns.
Scutellaria baicalensis seedlings were treated with BR and IAA solution with different concentrations by using foliage spray and root dipping patterns to study the effects of BR and IAA on growth,baicalin content and baicalein content of S.baicalensis seedlings and to provide a reference for production and quality improvement of S.baicalensis.Result:The treatment of BR and IAA solution with different concentrations under foliage spray and root dipping patterns has the obvious effect on growth and quality of S.baicalensis seedlings.And growth and quality of S.baicalensis seedlings treated with0.1 mg/L BR and 50 mg/L IAA is the best.The root-shoot ratio,plant height,lateral root number,leaf area index,photosynthetic rate,baicalin content and baicalein content of S.baicalensis seedlings treated with 0.1 mg/L BR and 50 mg/L IAA by the foliage spray pattern are 0.75,27.2 cm,17,8.03,20.58 mg/cm2·h,3.25% and 1.05%,257.14%,32.68%,88.89%,139.0%,118.24%,0.66% and0.35% higher than CK(water foliage spray treatment)respectively.The root-shoot ratio,plant height,lateral root number,leaf area index,photosynthetic rate,baicalin content and baicalein content of S.baicalensis seedlings treated with BR and IAA by the root dipping pattern are 0.78,26.7 cm,20,7.18,18.65 mg/cm2· h,3.12%and 1.03%,143.75%,31.53%,81.82%,246.86%,151.69%,0.55%and0.34% higher than CK(water root dipping treatment)separately.The suitable BR and IAA concentration under foliage spray and root dipping patterns can promote growth of S.baicalensis seedlings and improve quality and medicinal value of S.baicalensis significantly and there are no differences in growth and quality of S.baicalensis between two application patterns.
引文
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[5]张瑜,武斌.黄芩药理作用的研究进展[J].医学综述,2013,19(6):1091-1093.
[6]辛文妤,宋俊科,何国荣,等.黄芩素和黄芩苷的药理作用及机制研究进展[J].中国新药杂志,2013,22(6):647-653.
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[10]张琳.油菜素内酯的生理效应及发展前景[J].北方园艺,2011(20):188-191.
[11]VERT G,WALCHER C L,CHORY J,et al.Integration of auxin and brassinosteroid pathways by Auxin Response Factor[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(28):9829-9834.
[12]於维维,赵普庆,汪俏梅.油菜素甾醇类与生长素的相互作用[J].中国细胞生物学学报,2005,27(2):165-167.
[13]周晓艺,薛红卫.生长素与油菜素甾醇相互作用机制的研究进展[J].中国科学:生命科学,2013,43(12):1047-1053.
[14]赵普庆,於维维,汪俏梅.生长素与其他信号之间的相互作用[J].植物生理学报,2004,40(2):246-250.
[15]储昭庆,李李,宋丽,等.油菜素内酯生物合成与功能的研究进展[J].植物学报,2006,23(5):543-555.
[16]祁漫宇,朱维斌.叶面积指数主要测定方法和设备[J].安徽农业科学,2012(31):15097-15099.
[17]张志良,瞿伟菁.植物生理学实验指导[M].3版.北京:高等教育出版社,2003:185-189.
[18]潘鹏飞,孙菲,李延雪.HPLC法同时测定黄芩中黄芩苷、黄芩素、汉黄芩素的含量[J].中国药师,2012,15(1):75-77.
[19]李元元,曹清河.油菜素内酯参与调控植物生长发育与抗逆性的机制及其育种应用研究[J].中国农业科技导报,2015(2):25-32.
[20]罗琎,孙长忠,王琦,等.根系的发育及其激素调控研究[J].安徽农业科学,2008,36(26):11219-11222.
[21]周倩耘,丁家宜,刘峻,等.油菜素内酯对西洋参毛状根的生长和皂甙含量的影响[J].植物生理学报,2003,39(3):193-196.
[22]MEDFORD J I,ELMER J S,KLEE H J.Molecular cloning and characterization of genes expressed in shoot apical meristems[J].Plant Cell,2003,3(4):359-370.
[23]张志勇,王素芳,田晓莉,等.生长素调节植物侧根发育过程的机制[J].作物杂志,2009(1):11-13.
[24]维言,冯斗,徐建云.苗期喷施芸苔素对花生生长的影响及其生理效应[J].花生学报,2001(2):1-3.
[2]胡国强,张学文,李旻辉,等.植物生长调节剂缩节胺对黄芩活性成分含量的影响[J].中国中药杂志,2012,37(21):3215-3218.
[3]吴莹,金叶智,吴珺,等.黄芩主要成分体外抗甲型流感病毒作用的研究[J].北京中医药大学学报,2010,33(8):541-545.
[4]蔡葛平,郭燕红,姚辉,等.矮壮素和赤霉素对黄芩生物量及根中黄酮类成分产量的影响[J].中国农学通报,2008,24(7):213-217.
[5]张瑜,武斌.黄芩药理作用的研究进展[J].医学综述,2013,19(6):1091-1093.
[6]辛文妤,宋俊科,何国荣,等.黄芩素和黄芩苷的药理作用及机制研究进展[J].中国新药杂志,2013,22(6):647-653.
[7]尹昌喜,汪献芳,曾汉来,等.生长素对植物茎伸长的调控作用[J].植物生理学报,2009,45(5):503-508.
[8]吕习栋,袁留斌.油菜素内酯研究进展[J].农业灾害研究,2013(1):56-57.
[9]郑洁,王磊.油菜素内酯在植物生长发育中的作用机制研究进展[J].中国农业科技导报,2014,16(1):52-58.
[10]张琳.油菜素内酯的生理效应及发展前景[J].北方园艺,2011(20):188-191.
[11]VERT G,WALCHER C L,CHORY J,et al.Integration of auxin and brassinosteroid pathways by Auxin Response Factor[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(28):9829-9834.
[12]於维维,赵普庆,汪俏梅.油菜素甾醇类与生长素的相互作用[J].中国细胞生物学学报,2005,27(2):165-167.
[13]周晓艺,薛红卫.生长素与油菜素甾醇相互作用机制的研究进展[J].中国科学:生命科学,2013,43(12):1047-1053.
[14]赵普庆,於维维,汪俏梅.生长素与其他信号之间的相互作用[J].植物生理学报,2004,40(2):246-250.
[15]储昭庆,李李,宋丽,等.油菜素内酯生物合成与功能的研究进展[J].植物学报,2006,23(5):543-555.
[16]祁漫宇,朱维斌.叶面积指数主要测定方法和设备[J].安徽农业科学,2012(31):15097-15099.
[17]张志良,瞿伟菁.植物生理学实验指导[M].3版.北京:高等教育出版社,2003:185-189.
[18]潘鹏飞,孙菲,李延雪.HPLC法同时测定黄芩中黄芩苷、黄芩素、汉黄芩素的含量[J].中国药师,2012,15(1):75-77.
[19]李元元,曹清河.油菜素内酯参与调控植物生长发育与抗逆性的机制及其育种应用研究[J].中国农业科技导报,2015(2):25-32.
[20]罗琎,孙长忠,王琦,等.根系的发育及其激素调控研究[J].安徽农业科学,2008,36(26):11219-11222.
[21]周倩耘,丁家宜,刘峻,等.油菜素内酯对西洋参毛状根的生长和皂甙含量的影响[J].植物生理学报,2003,39(3):193-196.
[22]MEDFORD J I,ELMER J S,KLEE H J.Molecular cloning and characterization of genes expressed in shoot apical meristems[J].Plant Cell,2003,3(4):359-370.
[23]张志勇,王素芳,田晓莉,等.生长素调节植物侧根发育过程的机制[J].作物杂志,2009(1):11-13.
[24]维言,冯斗,徐建云.苗期喷施芸苔素对花生生长的影响及其生理效应[J].花生学报,2001(2):1-3.