不同品种烤烟根际微生物群落结构及多样性研究
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摘要
[目的]研究不同品种烤烟根际微生物群落结构及多样性差异。[方法]以不同烤烟品种云烟87、云烟97、云烟99和K326为材料,在2015和2016年分别进行田间试验,通过Biolog方法比较不同烤烟品种之间根际土壤微生物群落结构及多样性差异。[结果]土壤微生物群落代谢活性表现为K326>Y97>Y87>Y99;云烟99根际微生物对羧酸类碳源利用最多,而云烟87、云烟97、K326根际微生物对碳水化合物碳源利用最多;主成分分析结果显示,碳水化合物、羧酸、氨基酸与第一主成分(PC1)相关性较高,这3类碳源是区分云烟99与其他3个品种间差异的敏感碳源;不同品种的烤烟,其根际微生物种类和优势种群数量并无差异,但微生物种群的分布情况存在显著差异,尤以K326根际微生物群落物种分布最均匀。[结论]该研究为改善烤烟根际土壤微生态提供理论依据。
[Objective] To study microbial community structure and diversity in rhizosphere of different flue-cured tobacco. [Method]Field experiments were conducted in 2015 and 2016 respectively to study the difference of rhizosphere microbial community structure and functional diversity with Biolog technique among Yunyan 87,Yunyan 97,Yunyan 99 and K326 four flue-cured tobacco cultivars. [Result]Metabolic activity of the soil microbial communities followed the order: K326>Y97>Y87>Y99. Carboxylic acids was the most used carbon source by Yunyan99,but carbonhydrates was the most used carbon source by Yunyan 87,Yunyan 97,K326. The result of principal component analysis showed that these 3 types of carbon source were carbonhydrates,carboxylic acids and amino acids,Which had a high relevance of PC1 and that were used to distinguish Yunyan 99 and other. There was no difference for different flue-cured tobaccos between microbial species and dominant population,but there was significant differences in the distribution of microorganism population. The most uniform was K326.[Conclusion]This study provides theoretical basis for improving the soil microecology of flue-cured tobacco rhizosphere.
[Objective] To study microbial community structure and diversity in rhizosphere of different flue-cured tobacco. [Method]Field experiments were conducted in 2015 and 2016 respectively to study the difference of rhizosphere microbial community structure and functional diversity with Biolog technique among Yunyan 87,Yunyan 97,Yunyan 99 and K326 four flue-cured tobacco cultivars. [Result]Metabolic activity of the soil microbial communities followed the order: K326>Y97>Y87>Y99. Carboxylic acids was the most used carbon source by Yunyan99,but carbonhydrates was the most used carbon source by Yunyan 87,Yunyan 97,K326. The result of principal component analysis showed that these 3 types of carbon source were carbonhydrates,carboxylic acids and amino acids,Which had a high relevance of PC1 and that were used to distinguish Yunyan 99 and other. There was no difference for different flue-cured tobaccos between microbial species and dominant population,but there was significant differences in the distribution of microorganism population. The most uniform was K326.[Conclusion]This study provides theoretical basis for improving the soil microecology of flue-cured tobacco rhizosphere.
引文
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[18]毛家伟,张翔,叶红朝,等.土壤改良剂配施氮肥对烤烟SPAD值、根系活力及经济性状的影响[J].江苏农业科学,2014,42(10):112-114.
[19]王戈,杨焕文,赵正雄,等.不同抗性烤烟品种根际微生物数量及多样性差异研究[J].植物营养与肥料学报,2012,18(2):451-458.
[20]蔡秋华,左进香,李忠环,等.抗性烤烟品种根际微生物数量及功能多样性差异[J].应用生态学报,2015,26(12):3766-3772.
[21]许玲.烤烟品种和生长势对节肢动物群落动态的影响[D].杨凌:西北农林科技大学,2010.
[2]康林玉,刘周斌,欧立军,等.土壤微生物促进作物生长发育研究进展[J].湖南农业科学,2017(3):113-116.
[3]孙丹平.稻田水旱复种轮作对作物生长、资源利用及土壤生态环境的影响[D].南昌:江西农业大学,2016.
[4]杨梅.四川省主要植烟区土壤生态化学计量学特征研究[D].雅安:四川农业大学,2015.
[5]李敏,李琴,赵丽娜,等.我国土壤环境保护标准体系优化研究与建议[J].环境科学研究,2016,29(12):1799-1810.
[6]李昊.许昌市襄城县大田烟草病虫害发生与防治情况调查[D].郑州:河南农业大学,2016.
[7]李世金,刘国侠,张国.不同施氮量及种植密度对云烟99农艺性状和经济性状的影响[J].安徽农学通报,2017,23(24):45,52.
[8]杜坚,王珂清,张建强,等.我国不同生态区云烟87烟叶主要化学成分及感官风格差异[J].中国烟草科学,2018,39(2):96-102.
[9]王政,王学杰,王崇玉.不同海拔高度云烟97生长发育差异比较[J].山东农业科学,2016,48(5):35-38.
[10]尤垂淮,江丽芳,席飞虎,等.不同生态区烤烟K326质量特征差异分析[J].福建农林大学学报(自然科学版),2016,45(3):241-246.
[11]GARLAND J L,MILLS A L.Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization[J].Applied&environmental microbiology,1991,57(8):2351-2359.
[12]YOU M Y,YUAN Y R,LI L J,et al.Soil CO2emissions as affected by 20-year continuous cropping in mollisols[J].Journal of integrative agriculture,2014,13(3):615-623.
[13]宋收,陈晓明,肖伟,等.基于BIOLOG指纹解析土壤可培微生物对铀污染的响应[J].核农学报,2016,30(6):1169-1177.
[14]程存刚,赵德英,吕德国,等.植物源有机物料对果园土壤微生物群落多样性的影响[J].植物营养与肥料学报,2014(4):913-922.
[15]杨旭初,龙莉,屠乃美,等.微生物肥料在不同节肥水平下对烟草根际微生物碳代谢指纹的影响[J].核农学报,2017,31(10):2016-2022.
[16]熊定鹏,赵广帅,武建双,等.羌塘高寒草地物种多样性与生态系统多功能关系格局[J].生态学报,2016,36(11):3362-3371.
[17]张仕颖,夏运生,肖炜,等.除草剂丁草胺对高产水稻土微生物群落功能多样性的影响[J].生态环境学报,2013,22(5):815-819.
[18]毛家伟,张翔,叶红朝,等.土壤改良剂配施氮肥对烤烟SPAD值、根系活力及经济性状的影响[J].江苏农业科学,2014,42(10):112-114.
[19]王戈,杨焕文,赵正雄,等.不同抗性烤烟品种根际微生物数量及多样性差异研究[J].植物营养与肥料学报,2012,18(2):451-458.
[20]蔡秋华,左进香,李忠环,等.抗性烤烟品种根际微生物数量及功能多样性差异[J].应用生态学报,2015,26(12):3766-3772.
[21]许玲.烤烟品种和生长势对节肢动物群落动态的影响[D].杨凌:西北农林科技大学,2010.