沼液中添加某些活性成分对交链孢菌的抑制影响
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摘要
为了探究在沼液中添加某些活性成分对沼液抑制交链孢菌效果的影响,该实验采用抑菌效果相对较好的青蒿作为发酵原料经中温厌氧消化产生沼液,然后在沼液中添加不同浓度的腐殖酸,赤霉素,VB_1,VB_2,VB_6及(NH_4)_2SO_4,观察添加不同浓度的活性成分对抑制交链孢菌的影响。结果表明:在沼液中添加不同浓度的腐殖酸,赤霉素,VB_1,VB_2及(NH_4)_2SO_4都不同程度地增加了对交链孢菌的抑制效果,各种活性成分的最适抑制效果的浓度依次是3%,0.4%,0.1%,0.4%,1%,但是低浓度的VB_6对沼液抑菌的效果并无任何影响,高浓度的VB_6反而减弱了青蒿沼液对交链孢菌的抑制效果。
In order to explore the effect of adding some active components on the inhibition of Alternaria spp in biogas slurry,a anaerobic digestion experiment carried Artemisia apiacea as a fermentation raw material,which had relatively good bacteriostatic effect,and the mesophilic temperature was adopted. The produced biogas slurry was then added by different concentration of humic acid,gibberellin,VB_1,VB_2,VB_6 and( NH_4)_2SO_4. The effects of different concentration of active ingredients on inhibition of Alternaria spp. were observed. The results showed that adding humic acid,gibberellin,VB_1,VB_2 and( NH_4)_2SO_4 in biogas slurry increased the inhibitory effect on Alternaria spp in different degrees,their optimum inhibitory effect was 3%,0. 4%,0. 1%,0. 4% and 1% respectively. However,low concentration of VB_6 did not have any effect on the bacteria inhibition in biogas slurry,and high concentration of VB_6 actually weakened the alternaria spp inhibition effect of Artemisia apiacea.
In order to explore the effect of adding some active components on the inhibition of Alternaria spp in biogas slurry,a anaerobic digestion experiment carried Artemisia apiacea as a fermentation raw material,which had relatively good bacteriostatic effect,and the mesophilic temperature was adopted. The produced biogas slurry was then added by different concentration of humic acid,gibberellin,VB_1,VB_2,VB_6 and( NH_4)_2SO_4. The effects of different concentration of active ingredients on inhibition of Alternaria spp. were observed. The results showed that adding humic acid,gibberellin,VB_1,VB_2 and( NH_4)_2SO_4 in biogas slurry increased the inhibitory effect on Alternaria spp in different degrees,their optimum inhibitory effect was 3%,0. 4%,0. 1%,0. 4% and 1% respectively. However,low concentration of VB_6 did not have any effect on the bacteria inhibition in biogas slurry,and high concentration of VB_6 actually weakened the alternaria spp inhibition effect of Artemisia apiacea.
引文
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[2]刘万德,苏建荣,王梦君,等.滇西北云南红豆杉种群空间分布格局[J].西北林学院学报,2012,27(6):131-138.
[3]苏建荣,臧传富,刘万德,等.光质对云南红豆杉量影响的研究[J].林业科学研究,2012,25(4):419-424.
[4]赵永生,赵俊雄,张静华,等.云南红豆杉资源保护与开发利用前景[J].中国民族民间医药,2011,(13):28,31.
[5]谢美华,李霖,李雪玲,等.云南红豆杉叶斑病病原菌的分离鉴定和生物学特性[J].江苏农业科学,2014,42(11):146-149.
[6]张无敌.沼气发酵残留物利用基础[M].昆明:云南科技出版社,2002.
[7]刘继红,胡涵,张加稳,等.不同发酵原料沼液对交链孢菌抑制作用的研究[J].云南师范大学学报(自然科学版),2017,37(3):15-19.
[8]张无敌,刘士清,赖建华,等.厌氧消化残留物在防治农作物病虫害中的作用[J].中国沼气,1996,14(1):6-9.
[9]Mengying F,Lichun L,Fang Y et al.The Experimental Study on the inhibition Effect of Fusarium oxyspomm with Bio-slurry based on Material Properties[J].Advanced Materials Research,2013,675:317-321.
[10]尹芳,张无敌,刘士清,等.沼液抑制三七镰刀菌的影响因素研究[J].中国沼气,2006,24(2):51-52,62.
[11]陈丽琼,尹芳,刘士清,等.沼液与8种农药对烟草赤星病抑制效果的对比研究[J].中国沼气,2006,24(3):16-18,49.
[12]赵恭文,徐延熙,朱长华,等.牛粪沼液对6种植物病原真菌的抑制作用[J].中国沼气,2012,30(5):35-37.
[13]纪钧麟,李忠华,张加稳,等.沼液与10种农药对云南红豆杉叶斑病菌的抑制效果[J].云南师范大学学报(自然科学版),2016,36(6):12-16.
[14]方梦萦,张无敌,尹芳,等.不同来源沼液对根霉抑制作用的研究[J].云南师范大学学报(自然科学版),2014,34(3):29-32.
[15]方梦萦,张无敌,尹芳,等.沼气发酵液对烟草赤星菌抑菌作用的研究[J].云南师范大学学报(自然科学版),2011,31(增刊):115-118.
[16]曹云,常志州,马艳,等,猪粪沼液防治辣椒疫病机理研究_沼液中铵与腐殖酸的作用[J].中国生态农业学报,2013,21(9):1119-1126.
[17]Valdrighi M M,Pera A,Agnolucci M,et al.Effects of compost-derived humic acids on vegetable biomass production and microbial growth within a plant(Cichorium intybus)-soil system:A comparative study[J].Agriculture Ecosystems Environment,1996,58(2/3):133-144.