水中病毒的浓缩检测与处理技术研究进展
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摘要
水环境中的病毒需要先进行浓缩富集才可以进行检测,介绍了目前常见的病毒浓集方法,包括滤膜过滤法、吸附-洗脱法、絮凝沉淀法、超速离心法、免疫磁珠分离法、超滤法。综述了4种病毒检测方法:细胞培养法、免疫学检测法、PCR检测法、生物芯片技术;处理实际水环境中的病毒可通过两个途径:分离和灭活;最后对水环境病毒的研究现状进行了展望。
Before detecting,the virus in the aquatic environment needs to be concentrated,the article introduces the current common virus concentration methods,including membrane filtration method,adsorption-elution method,flocculent precipitation,ultracentrifugation,immunomagnetic bead,ultrafiltration;This paper summarizes the four viral detection methods:cell culture method,immunological detection method,PCR detection method,biochip technology;The virus in the actual water environment can be treated through two ways:separation and inactivation;Furthermore,the research status of the virus in aquatic environment is discussed.
Before detecting,the virus in the aquatic environment needs to be concentrated,the article introduces the current common virus concentration methods,including membrane filtration method,adsorption-elution method,flocculent precipitation,ultracentrifugation,immunomagnetic bead,ultrafiltration;This paper summarizes the four viral detection methods:cell culture method,immunological detection method,PCR detection method,biochip technology;The virus in the actual water environment can be treated through two ways:separation and inactivation;Furthermore,the research status of the virus in aquatic environment is discussed.
引文
[1] Pedley S,Pond K.Emerging Issues in Water and Infectious Disease[M].Switzerland:WHO,2003.
[2] Units T.Guidelines for Drinking-Water Quality[M].Fourth Edition.Switzerland:WHO,2011.
[3] Gall A M,Marias B J,Lu Y,et al.Waterborne viruses:A barrier to safe drinking water[J].PLoS Pathog,2015,11(6):e1004867.
[4] Kim S H,Cheon D S,Kim J H,et al.Outbreaks of gastroenteritis that occurred during school excursions in Korea were associated with several waterborne strains of norovirus[J].Journal of Clinical Microbiology,2005,43(9):4836-4839.
[5] Ye X Y,Ming X,Zhang Y L,et al.Real-time PCR detection of enteric viruses in source water and treated drinking water in Wuhan,China[J].Current Microbiology,2012,65(3):244-253.
[6] 秀云.水环境中的病毒[J].环境保护,1982(3):20-22.
[7] Wommack K E,Hill R T,Colwell R R.A simple method for the concentration of viruses from natural water samples[J].Journal of Microbiological Methods,1995,22(1):57-67.
[8] 张崇淼,刘永军,王晓昌,等.环境水体中肠道病毒的膜吸附-洗脱浓缩方法研究[J].环境科学,2007,28(7):1543-1547.
[9] Karim M R,Rhodes E R,Brinkman N,et al.New electropositive filter for concentrating enteroviruses and noroviruses from large volumes of water[J].Applied and Environmental Microbiology,2009,75(8):2393-2399.
[10] Jothikumar N,Khanna P,Paulmurugan R,et al.A simple device for the concentration and detection of enterovirus,hepatitis E virus and rotavirus from water samples by reverse transcription-polymerase chain reaction[J].Journal of Virological Methods,1995,55(3):401-415.
[11] Gantzer C,Senouci S,Maul A,et al.Enterovirus genomes in wastewater:concentration on glass wool and glass powder and detection by RT-PCR[J].Journal of Virological Methods,1997,65(2):265-271.
[12] 李丹.水中病毒分析方法及水处理过程病毒去除特性研究[D].北京:清华大学,2010.
[13] Calgua B,Rodriguez-Manzano J,Hundesa A,et al.New methods for the concentration of viruses from urban sewage using quantitative PCR[J].Journal of Virological Methods,2013,187(2):215-221.
[14] 潘銮凤.离心技术及其应用[C]//2008华东生物物理与分子生物学及其在医药、农畜、食品科学中应用学术会议论文集.上海:上海生物物理学会,2008.
[15] Murphy A M,Grohmann G S,Sexton M F H.Infectious gastroenteritis in Norfolk Island and recovery of viruses from drinking water[J].Journal of Hygiene,1983,91(1):139-146.
[16] Gilpatrick S G,Schwab K J,Estes M K,et al.Development of an immunomagnetic capture reverse transcription-PCR assay for the detection of Norwalk virus[J].Journal of Virological Methods,2000,90(1):69-78.
[17] 杨万,何苗.免疫磁珠分离技术在环境病原微生物检测中的应用[J].安全与环境学报,2008,8(6):18-22.
[18] Haramoto E,Katayama H,Oguma K,et al.One-year monthly monitoring of Torque teno virus (TTV) in wastewater treatment plants in Japan[J].Water Research,2005,39(10):2008-2013.
[19] Haramoto E,Katayama H,Ohgaki S.Detection of noroviruses in tap water in Japan by means of a new method for concentrating enteric viruses in large volumes of freshwater[J].Appl Environ Microbiol,2004,70(4):2154-2160.
[20] 张文福.病毒灭活检验技术[J].中国消毒学杂志,2004,21(3):260-261.
[21] Fong T T,Lipp E K.Enteric viruses of humans and animals in aquatic envimnments:health risks,detection,and potential water quality assessment tools[J].Microbiol M of Biol Rev,2005,69(2):357-377.
[22] Espinoza J Z,Kuznar J.Rapid simultaneous detection and quantitation of infectious pancreatic necrosis virus (IPNV)[J].Journal of Virological Methods,2002,105(1):81-85.
[23] 文凯良,唐爱国.SARS病毒检测的研究进展[J].医学临床研究,2003,20(7):518-520.
[24] Castignolles N,Petit F,Mendel I,et al.Detection of adenovirus in the waters of the Seine River estuary by nested-PCR[J].Molecular & Cellular Probes,1998,12(3):175-180.
[25] Schvoerer E,Bonnet F,Dubois V,et al.PCR detection of human enteric viruses in bathing areas,waste waters and human stools in Southwestern France[J].Research in Microbiology,2000,151(8):693-701.
[26] Taylor M B,Cox N,Vrey M A,et al.The occurrence of hepatitis A and astroviruses in selected river and dam waters in South Africa[J].Water Research,2001,35(11):2653-2660.
[27] Soule H,Genoulaz O,Gratacap-Cavallier B,et al.Ultrafiltration and reverse transcription-polymerase chain reaction:an efficient process for poliovirus,rotavirus and hepatitis A virus detection in water[J].Water Research,2000,34(3):1063-1067.
[28] 美国环保局组织.污水再生利用指南[M].胡洪营,魏东斌,王丽莎,等,译.北京:化学工业出版社,2008.
[29] 陈军红.空气和水环境中病毒的富集、检测与净化[D].杭州:浙江大学,2005.
[30] 吉铮.城市污水及再生水中典型病毒的赋存及分布特性研究[D].西安:西安建筑科技大学,2014.
[31] Hijnen W A,Beerendonk E F,Medema G J.Inactivation credit of UV radiation for viruses,bacteria and protozoan (oo)cysts in water:a review[J].Water Research,2006,40(2):3-22.
[32] Nuanualsuwan S,Cliver D O.Infectivity of RNA from inactivated poliovirus[J].Applied and Environmental Microbiology,2003,69:1629-1632.
[33] Churn C C,Bates R C,Boardman G D.Mechanism of chlorine inactivation of DNA-containing Parvovirus-H-1[J].Applied and Environmental Microbiology,1983,46:1394-1402.
[34] 赵祖国.污水中病毒浓集、消毒规律及灭活机理研究[D].天津:中国人民解放军军事医学科学院,2008.
[35] Tsai C T,Lin S T.Disinfection of hospital waste sludge using hydrochlorite and chlorine dioxide[J].Journal of Applied Microbiology,1999,86:827-839.
[36] 顾霓涛,龚天翼,乔宇祥,等.纳米TiO2-光催化灭活污水厂出水病毒的研究[J].中国给水排水,2018,34(9):96-100.
[2] Units T.Guidelines for Drinking-Water Quality[M].Fourth Edition.Switzerland:WHO,2011.
[3] Gall A M,Marias B J,Lu Y,et al.Waterborne viruses:A barrier to safe drinking water[J].PLoS Pathog,2015,11(6):e1004867.
[4] Kim S H,Cheon D S,Kim J H,et al.Outbreaks of gastroenteritis that occurred during school excursions in Korea were associated with several waterborne strains of norovirus[J].Journal of Clinical Microbiology,2005,43(9):4836-4839.
[5] Ye X Y,Ming X,Zhang Y L,et al.Real-time PCR detection of enteric viruses in source water and treated drinking water in Wuhan,China[J].Current Microbiology,2012,65(3):244-253.
[6] 秀云.水环境中的病毒[J].环境保护,1982(3):20-22.
[7] Wommack K E,Hill R T,Colwell R R.A simple method for the concentration of viruses from natural water samples[J].Journal of Microbiological Methods,1995,22(1):57-67.
[8] 张崇淼,刘永军,王晓昌,等.环境水体中肠道病毒的膜吸附-洗脱浓缩方法研究[J].环境科学,2007,28(7):1543-1547.
[9] Karim M R,Rhodes E R,Brinkman N,et al.New electropositive filter for concentrating enteroviruses and noroviruses from large volumes of water[J].Applied and Environmental Microbiology,2009,75(8):2393-2399.
[10] Jothikumar N,Khanna P,Paulmurugan R,et al.A simple device for the concentration and detection of enterovirus,hepatitis E virus and rotavirus from water samples by reverse transcription-polymerase chain reaction[J].Journal of Virological Methods,1995,55(3):401-415.
[11] Gantzer C,Senouci S,Maul A,et al.Enterovirus genomes in wastewater:concentration on glass wool and glass powder and detection by RT-PCR[J].Journal of Virological Methods,1997,65(2):265-271.
[12] 李丹.水中病毒分析方法及水处理过程病毒去除特性研究[D].北京:清华大学,2010.
[13] Calgua B,Rodriguez-Manzano J,Hundesa A,et al.New methods for the concentration of viruses from urban sewage using quantitative PCR[J].Journal of Virological Methods,2013,187(2):215-221.
[14] 潘銮凤.离心技术及其应用[C]//2008华东生物物理与分子生物学及其在医药、农畜、食品科学中应用学术会议论文集.上海:上海生物物理学会,2008.
[15] Murphy A M,Grohmann G S,Sexton M F H.Infectious gastroenteritis in Norfolk Island and recovery of viruses from drinking water[J].Journal of Hygiene,1983,91(1):139-146.
[16] Gilpatrick S G,Schwab K J,Estes M K,et al.Development of an immunomagnetic capture reverse transcription-PCR assay for the detection of Norwalk virus[J].Journal of Virological Methods,2000,90(1):69-78.
[17] 杨万,何苗.免疫磁珠分离技术在环境病原微生物检测中的应用[J].安全与环境学报,2008,8(6):18-22.
[18] Haramoto E,Katayama H,Oguma K,et al.One-year monthly monitoring of Torque teno virus (TTV) in wastewater treatment plants in Japan[J].Water Research,2005,39(10):2008-2013.
[19] Haramoto E,Katayama H,Ohgaki S.Detection of noroviruses in tap water in Japan by means of a new method for concentrating enteric viruses in large volumes of freshwater[J].Appl Environ Microbiol,2004,70(4):2154-2160.
[20] 张文福.病毒灭活检验技术[J].中国消毒学杂志,2004,21(3):260-261.
[21] Fong T T,Lipp E K.Enteric viruses of humans and animals in aquatic envimnments:health risks,detection,and potential water quality assessment tools[J].Microbiol M of Biol Rev,2005,69(2):357-377.
[22] Espinoza J Z,Kuznar J.Rapid simultaneous detection and quantitation of infectious pancreatic necrosis virus (IPNV)[J].Journal of Virological Methods,2002,105(1):81-85.
[23] 文凯良,唐爱国.SARS病毒检测的研究进展[J].医学临床研究,2003,20(7):518-520.
[24] Castignolles N,Petit F,Mendel I,et al.Detection of adenovirus in the waters of the Seine River estuary by nested-PCR[J].Molecular & Cellular Probes,1998,12(3):175-180.
[25] Schvoerer E,Bonnet F,Dubois V,et al.PCR detection of human enteric viruses in bathing areas,waste waters and human stools in Southwestern France[J].Research in Microbiology,2000,151(8):693-701.
[26] Taylor M B,Cox N,Vrey M A,et al.The occurrence of hepatitis A and astroviruses in selected river and dam waters in South Africa[J].Water Research,2001,35(11):2653-2660.
[27] Soule H,Genoulaz O,Gratacap-Cavallier B,et al.Ultrafiltration and reverse transcription-polymerase chain reaction:an efficient process for poliovirus,rotavirus and hepatitis A virus detection in water[J].Water Research,2000,34(3):1063-1067.
[28] 美国环保局组织.污水再生利用指南[M].胡洪营,魏东斌,王丽莎,等,译.北京:化学工业出版社,2008.
[29] 陈军红.空气和水环境中病毒的富集、检测与净化[D].杭州:浙江大学,2005.
[30] 吉铮.城市污水及再生水中典型病毒的赋存及分布特性研究[D].西安:西安建筑科技大学,2014.
[31] Hijnen W A,Beerendonk E F,Medema G J.Inactivation credit of UV radiation for viruses,bacteria and protozoan (oo)cysts in water:a review[J].Water Research,2006,40(2):3-22.
[32] Nuanualsuwan S,Cliver D O.Infectivity of RNA from inactivated poliovirus[J].Applied and Environmental Microbiology,2003,69:1629-1632.
[33] Churn C C,Bates R C,Boardman G D.Mechanism of chlorine inactivation of DNA-containing Parvovirus-H-1[J].Applied and Environmental Microbiology,1983,46:1394-1402.
[34] 赵祖国.污水中病毒浓集、消毒规律及灭活机理研究[D].天津:中国人民解放军军事医学科学院,2008.
[35] Tsai C T,Lin S T.Disinfection of hospital waste sludge using hydrochlorite and chlorine dioxide[J].Journal of Applied Microbiology,1999,86:827-839.
[36] 顾霓涛,龚天翼,乔宇祥,等.纳米TiO2-光催化灭活污水厂出水病毒的研究[J].中国给水排水,2018,34(9):96-100.