白介素17受体mRNA在恒河猴中的分布
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摘要
目的:阐述白介素17受体(IL-17R)mRNA在恒河猴不同组织中的分布与丰度。方法:建立实时荧光定量PCR方法,检测免疫、消化和生殖等系统的各组织中IL-17RA mRNA的水平,并检测了肠道IL-17RC mRNA的水平及恒河猴、非洲绿猴和食蟹猴PBMC中IL-17RA和IL-17RC mRNA的表达水平。结果:IL-17RA mRNA在所有检测过的组织中均有表达,但不同器官系统的表达水平存在显著差异(P<0.05);IL-17RC mRNA在大肠中的表达水平高于小肠;恒河猴PBMC中IL-17RA mRNA水平显著高于非洲绿猴(5倍)和食蟹猴(3倍),但PBMC中IL-17RC mRNA水平在3种动物中均低于检测下限。结论:IL-17RA广泛分布于恒河猴各种组织中,表达水平存在明显的组织差异和种属差异。
Objective: To delineate the distribution and abundance of interleukin 17 receptor mRNA in normal tissues of rhesus macaques. Methods: Tissue samples were collected from different systems including lymphoid system,digestive system and genital system and the mRNA levels of IL-17 RA and IL-17 RC were examined by real time RT-PCR. Meanwhile the levels of IL-17 RA and IL-17 RC mRNA in the PBMCs of rhesus macaques,African green monkeys and cynomolgus monkeys were examined. Results: IL-17 RA mRNA was expressed in all the tested tissues and the levels were significantly different among these systems( P<0. 05). IL-17 RC mRNA level in the large intestine was higher than that in the small intestine. IL-17 RA mRNA level in rhesus macaque PBMCs was 3 and 5 times higher than that of cynomolgus monkeys and African green monkeys respectively,while IL-17 RC mRNA in the PBMCs was below the limit of detection. Conclusion: IL-17 RA is widely distributed in rhesus macaques; the expression level is different among different tissues and species.
Objective: To delineate the distribution and abundance of interleukin 17 receptor mRNA in normal tissues of rhesus macaques. Methods: Tissue samples were collected from different systems including lymphoid system,digestive system and genital system and the mRNA levels of IL-17 RA and IL-17 RC were examined by real time RT-PCR. Meanwhile the levels of IL-17 RA and IL-17 RC mRNA in the PBMCs of rhesus macaques,African green monkeys and cynomolgus monkeys were examined. Results: IL-17 RA mRNA was expressed in all the tested tissues and the levels were significantly different among these systems( P<0. 05). IL-17 RC mRNA level in the large intestine was higher than that in the small intestine. IL-17 RA mRNA level in rhesus macaque PBMCs was 3 and 5 times higher than that of cynomolgus monkeys and African green monkeys respectively,while IL-17 RC mRNA in the PBMCs was below the limit of detection. Conclusion: IL-17 RA is widely distributed in rhesus macaques; the expression level is different among different tissues and species.
引文
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[19]Giles DA,Moreno-Fernandez ME,Stankiewicz TE,et al.Regulation of inflammation by IL-17A and IL-17F modulates nonalcoholic fatty liver disease pathogenesis[J].PLo S One,2016,11(2):e0149783.
[20]Norley S,Kurth R.The role of the immune response during SIVagm infection of the African green monkey natural host[J].Front Biosci,2004,9(71):550-564.
[21]Taylor PR,Roy S,Leal SM Jr,et al.Activation of neutrophils by autocrine IL-17A-IL-17RC interactions during fungal infection is regulated by IL-6,IL-23,RORγt and dectin-2[J].Nat Immunol,2014,15(2):143-151.
[2]Hymowitz SG,Filvaroff EH,Yin JP,et al.IL-17s adopt a cystine knot fold:structure and activity of a novel cytokine,IL-17F,and implications for receptor binding[J].Embo J,2001,20(19):5332-5341.
[3]Gaffen SL.Structure and signalling in the IL-17 receptor family[J].Nat Rev Immunol,2009,9(11):556-567.
[4]Cortez DM,Feldman MD,Mummidi S,et al.IL-17 stimulates MMP-1 expression in primary human cardiac fibroblasts via p38 MAPK-and ERK1/2-dependent C/EBP-beta,NF-kappa B,and AP-1 activation[J].Am J Physiol Heart Circ Physiol,2007,293(6):H3356-3365.
[5]Kao CY,Huang F,Chen Y,et al.Up-regulation of CC chemokine ligand 20 expression in human airway epithelium by IL-17 through a JAK-independent but MEK/NF-kappa B-dependent signaling pathway[J].J Immunol,2005,175(10):6676-6685.
[6]Tsai HC,Velichko S,Hung LY,et al.IL-17A and Th17 cells in lung inflammation:an update on the role of Th17 cell differentiation and IL-17R signaling in host defense against infection[J].Clin Developmental Immunol,2013,2013(5):267971.
[7]Mcaleer JP,Kolls JK.Directing traffic:IL-17 and IL-22 coordinate pulmonary immune defense[J].Immunol Rev,2014,260(1):129-144.
[8]Yu JJ,Gaffen SL.Interleukin-17:a novel inflammatory cytokine that bridges innate and adaptive immunity[J].Front Biosci,2008,13(13):170-177.
[9]Yao Z,Fanslow WC,Seldin MF,et al.Herpesvirus Saimiri encodes a new cytokine,IL-17,which binds to a novel cytokine receptor[J].Immunity,2011,187(9):4392-4402.
[10]Ishigame H,Kakuta S,Nagai T,et al.Differential roles of interleukin-17A and-17F in host defense against mucoepithelial bacterial infection and allergic responses[J].Immunity,2009,30(1):108-119.
[11]Crowe CR,Chen K,Pociask DA.Critical role of IL-17RA in immunopathology of influenza infection[J].J Immunol,2009,183(8):5301-5310.
[12]Rafa H,Saoula H,Belkhelfa M,et al.IL-23/IL-17A axis correlates with the nitric oxide pathway in inflammatory bowel disease:immunomodulatory effect of retinoic acid[J].J Interferon Cytokine Res,2013,33(7):355-368.
[13]Jiang YX,Li PA,Yang SW,et al.Increased chemokine receptor IL-17RA expression is associated with poor survival in gastric cancer patients[J].Intern J Clin Exp Pathol,2015,8(6):7002-7008.
[14]Zhang WJ,Wang Y,Yu K,et al.Associated changes in the transcription levels of IL-17A and tight junction-associated genes in the duodenal mucosa of rhesus macaques repeatedly exposed to simian/human immunodeficiency virus[J].Exp Mole Pathol,2014,97(2):225-233.
[15]邱趁丽,赵辉,杨贵波.中国恒河猴(Macaca mulatta)外周血Th17细胞及其在SHIV感染后的变化[J].细胞与分子免疫学杂志,2008,24(3):217-220.Qiu CL,Zhao H,Yang GB.Th17 lymphocytes in the peripheral bolld of Chinese rhesus macaques(Macaca mulatta)with or without SHIV infection[J].J Cell Mole Immunol,2008,24(3):217-220.
[16]Lee JS,Tato CM,Joyce-shaikh B,et al.Interleukin-23-independent IL-17 production regulates intestinal epithelial permeability[J].Immunity,2015,43(4):727-738.
[17]Beagley KW,Fujihashi K,Lagoo AS,et al.Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine[J].J Immunol,1995,154(11):5611-5619.
[18]Lombard R,Doz E,Carreras F,et al.IL-17RA in nonhematopoietic cells controls CXCL-1 and 5 critical to recruit neutrophils to the lung of mycobacteria-infected mice during the adaptive immune response[J].PLo S One,2016,11(2):e0149455.
[19]Giles DA,Moreno-Fernandez ME,Stankiewicz TE,et al.Regulation of inflammation by IL-17A and IL-17F modulates nonalcoholic fatty liver disease pathogenesis[J].PLo S One,2016,11(2):e0149783.
[20]Norley S,Kurth R.The role of the immune response during SIVagm infection of the African green monkey natural host[J].Front Biosci,2004,9(71):550-564.
[21]Taylor PR,Roy S,Leal SM Jr,et al.Activation of neutrophils by autocrine IL-17A-IL-17RC interactions during fungal infection is regulated by IL-6,IL-23,RORγt and dectin-2[J].Nat Immunol,2014,15(2):143-151.