比格犬深静脉穿刺置管模型的建立及应用
|
摘要
目的建立比格犬深静脉穿刺置管模型,探讨其应用范围。方法将8只成年比格犬随机分成股静脉穿刺置管组(股静脉组)和颈内静脉穿刺置管组(颈内静脉组),每组4只。对比格犬行1.5%~3%异氟烷和氧气混合吸入麻醉后,取仰卧位固定于手术台上,定位股静脉或颈内静脉,行经皮深静脉穿刺置管术并固定导管。结果行深静脉穿刺置管术的8只比格犬全部置管成功,术中麻醉效果良好,股静脉穿刺置管较颈内静脉穿刺置管操作方便,手术时间短,术后便于护理,并发症少。结论比格犬深静脉穿刺置管模型的建立,实现了血液灌流及血液净化等技术在实验动物身上的应用,为开展相关临床基础研究提供方便,也可应用于兽医对中毒犬行血液灌流或血液净化,对休克犬进行快速大量补液等。
Objective To establish an animal model through DVPC in beagles and to discuss its field of application. Method Eight adult beagles were randomly divided into the group of femoral vein catheterization(FVC) and the group of jugular vein catheterization(JVC),each group with four beagles. After anesthesia with a mixture of 1. 5%-3%(v/v) isoflurane and oxygen,the beagles were fixed with a supine position in the operating table. The femoral vein and jugular were positioned to carry out DVPC and the catheters were fastened. Result DVPC for eight beagles was successful and the anesthetic effect was good. Compared with JVC,FVC operates more conveniently with shorter operating time,easier nursing and fewer complications. Conclusion The establishment of DVPC in beagles makes it possible for the application of blood perfusion and purification to laboratory animals.Meanwhile,it facilitates relevant clinical basic research. It can also be applied by vets to poisoned dogs for blood perfusion or purification and also to shock dogs for quick and mass fluid infusion.
Objective To establish an animal model through DVPC in beagles and to discuss its field of application. Method Eight adult beagles were randomly divided into the group of femoral vein catheterization(FVC) and the group of jugular vein catheterization(JVC),each group with four beagles. After anesthesia with a mixture of 1. 5%-3%(v/v) isoflurane and oxygen,the beagles were fixed with a supine position in the operating table. The femoral vein and jugular were positioned to carry out DVPC and the catheters were fastened. Result DVPC for eight beagles was successful and the anesthetic effect was good. Compared with JVC,FVC operates more conveniently with shorter operating time,easier nursing and fewer complications. Conclusion The establishment of DVPC in beagles makes it possible for the application of blood perfusion and purification to laboratory animals.Meanwhile,it facilitates relevant clinical basic research. It can also be applied by vets to poisoned dogs for blood perfusion or purification and also to shock dogs for quick and mass fluid infusion.
引文
[1]Byles P H,Dobkin A B,Jones D B.Comparative effects of prolonged anaesthesia on mature beagle dogs and young rhesus monkeys[J].Canadian Anaesthetists’Society Journal,1971,18(4):397-407.
[2]Bert B,Hartje I,Voigt J P,et al.Repeated anaesthesia with isoflurane and xylazine/levomethadone/fenpipramide premedication in female Beagle dogs:influence on general health and wellbeing[J].Laboratory Animals,2008,42(4):473-482.
[3]邓家波,牛李丽,赵波,等.吸入麻醉在动物园动物上的应用[J].动物医学进展,2006,27(S1):103-104.
[4]于布为,薛庆生,罗艳.吸入麻醉药物的研究进展[J].继续医学教育,2006,20(15):26-36.
[5]刘晶星,温娜,王海莲,等.深静脉置管的临床应用及护理进展[J].护理研究,2007,21(3):661-663.
[6]方喜业.医学实验动物学[M].北京:人民卫生出版社,1995:138.
[7]孙靖.实验动物学基础[M].北京:北京科学技术出版社,2005:65.
[8]杨幼明,庄云发,林远夫,等.Beagle犬繁育性能及血液学生化学测定[J].上海实验动物科学,1994,14(3,4):144-147.
[9]刘启德,许庆文,欧卫平,等.Beagle犬的生物学特性及在药理毒理试验中的应用[J].中药新药与临床药理,2000,11(3):91-94.
[10]Hanton G,Rabemam Pianina Y.The electro cardiogram of the Beagle dog:reference values and effect of sex,genetic strain,body position and heart rate[J].Lab Anim,2006,40(2):123-136.
[11]袁咏梅,宋文新.深静脉穿刺置管术临床护理体会[J].河南外科学杂志,2015,21(4):147.
[12]周友惠,舒勤.深静脉置管的常见并发症及护理措施[J].全科护理,2010,8(6):1474-1474.
[2]Bert B,Hartje I,Voigt J P,et al.Repeated anaesthesia with isoflurane and xylazine/levomethadone/fenpipramide premedication in female Beagle dogs:influence on general health and wellbeing[J].Laboratory Animals,2008,42(4):473-482.
[3]邓家波,牛李丽,赵波,等.吸入麻醉在动物园动物上的应用[J].动物医学进展,2006,27(S1):103-104.
[4]于布为,薛庆生,罗艳.吸入麻醉药物的研究进展[J].继续医学教育,2006,20(15):26-36.
[5]刘晶星,温娜,王海莲,等.深静脉置管的临床应用及护理进展[J].护理研究,2007,21(3):661-663.
[6]方喜业.医学实验动物学[M].北京:人民卫生出版社,1995:138.
[7]孙靖.实验动物学基础[M].北京:北京科学技术出版社,2005:65.
[8]杨幼明,庄云发,林远夫,等.Beagle犬繁育性能及血液学生化学测定[J].上海实验动物科学,1994,14(3,4):144-147.
[9]刘启德,许庆文,欧卫平,等.Beagle犬的生物学特性及在药理毒理试验中的应用[J].中药新药与临床药理,2000,11(3):91-94.
[10]Hanton G,Rabemam Pianina Y.The electro cardiogram of the Beagle dog:reference values and effect of sex,genetic strain,body position and heart rate[J].Lab Anim,2006,40(2):123-136.
[11]袁咏梅,宋文新.深静脉穿刺置管术临床护理体会[J].河南外科学杂志,2015,21(4):147.
[12]周友惠,舒勤.深静脉置管的常见并发症及护理措施[J].全科护理,2010,8(6):1474-1474.