基于HPLC波长切换法同时测定复方肉苁蓉合剂中3种成分含量
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摘要
目的:建立同时测定复方肉苁蓉合剂中毛蕊花糖苷、党参炔苷和丹酚酸B的HPLC方法。方法:色谱柱:Inertsil C18柱(250 mm×4.6 mm,5μm),流动相(乙腈-0.1%磷酸水溶液=19∶81);流速1.0 m L/min;柱温35℃;毛蕊花糖苷、党参炔苷和丹酚酸B检测波长分别为330、286、268 nm。结果:毛蕊花糖苷、党参炔苷和丹酚酸B分别在11.2～112、5.06～50.6、15.6～156μg/m L范围内与色谱峰峰面积线性关系较好,相关系数分别为0.9998、0. 9997、0.9998,加样回收率的平均值分别为99.11%、97.81%、98.30%,RSD分别为1.94%、2.03%、1.35%(n=6)。结论:所建立的HPLC含量测定方法快速、准确,可同时测定复方肉苁蓉合剂中毛蕊花糖苷、丹酚酸B和党参炔苷的含量,为复方肉苁蓉合剂的质量分析与控制提供一定的参考。
Objective To establish a HPLC method for simultaneously determining verbascoside,codonopsis and salvianolic acid B in Fufang Roucongrong mixture. Methods HPLC method was performed on a Inertsil C18 column( 250 mm×4.6 mm,5 μm) with mobile phase( acetonitrile-0.1% phosphoric acid solution = 19 ∶ 81),flow rate 1.0 m L/min and column temperature 35 ℃. The detection wavelengths of of verbascoside,codonopsis glycoside and salvianolic acid B were 330,286,268 nm respectively.Results Verbascoside,codonopsis glycoside and salvianolic acid B in the corresponded ranges of 11.2～ 112,5.06 ～ 50.6,15.6 ～ 156 μg/m L demonstrated good linear relationship with chromatographic peak area. The correlation coefficients were 0.9998,0.9997 and 0.9998,the average recoveries were 99.11%,97.81% and 98.30%,and RSDs were 1.94%,2.03% and 1.35%( n = 6),respectively. Conclusion The established HPLC method is rapid and accurate. It can simultaneously determine the content of verbascoside,codonopsis glycoside and salvianolic acid in Fufang Roucongrong mixture and provide a reference for the control and quality analysis of the compound.
Objective To establish a HPLC method for simultaneously determining verbascoside,codonopsis and salvianolic acid B in Fufang Roucongrong mixture. Methods HPLC method was performed on a Inertsil C18 column( 250 mm×4.6 mm,5 μm) with mobile phase( acetonitrile-0.1% phosphoric acid solution = 19 ∶ 81),flow rate 1.0 m L/min and column temperature 35 ℃. The detection wavelengths of of verbascoside,codonopsis glycoside and salvianolic acid B were 330,286,268 nm respectively.Results Verbascoside,codonopsis glycoside and salvianolic acid B in the corresponded ranges of 11.2～ 112,5.06 ～ 50.6,15.6 ～ 156 μg/m L demonstrated good linear relationship with chromatographic peak area. The correlation coefficients were 0.9998,0.9997 and 0.9998,the average recoveries were 99.11%,97.81% and 98.30%,and RSDs were 1.94%,2.03% and 1.35%( n = 6),respectively. Conclusion The established HPLC method is rapid and accurate. It can simultaneously determine the content of verbascoside,codonopsis glycoside and salvianolic acid in Fufang Roucongrong mixture and provide a reference for the control and quality analysis of the compound.
引文
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[2]许爱霞,张振明,葛斌,等.党参多糖抗衰老作用机制的实验研究[J].中国现代应用药学,2006,23(8):729-731.
[3] Du GH,Qiu Y,Zhang JT.Salvianolic acid B protects thememory function against transient cerebral ischemia inmice[J].J Asia Nat Prod Res,2002,2(2):145-152.
[4]王翰华,沈岳光,陆元勋.浅析中药质量标准中建立多指标含量测定体系[J].中医药导报,2010,16(10):95-96.
[5]林建云,易延逵,蔡光先,等.中药多指标质量控制体系的研究分析[J].辽宁中医药杂志,2013,40(8):1674-1 676.
[6]赵微,潘英妮.肉苁蓉苯乙醇苷类成分药理作用研究进展[J].亚太传统医药,2013,9(5):77-79.
[7]张鑫,李建宽,赵玉静,等.党参化学成分及其体外抗氧化活性分析[J].中国实验方剂学,2018,24(24):53-59.
[8]何敏,伍春,明海霞,等.甘肃党参水煎剂对D-半乳糖诱导衰老小鼠免疫功能的影响[J].细胞与分子免疫学杂志,2013,29(8):794-797.
[9]李社芳,苗灵娟,李宁,等.丹酚酸B对糖氧剥夺损伤大鼠海马神经干细胞增殖、凋亡和分化的影响[J].中国组织工程研究,2017,21(17):2 735-2 740.
[10]国家药典委员会.中华人民共和国药典一部[M].北京:中国医药科技出版社,2015:135-136.
[11]南国,张鹏,王萌,等.HPLC法测定稳心颗粒中三七皂苷R1、人参皂苷Rg1、Rb1、Rd与党参炔苷的含量[J].天津中医药,2016,7(33):434-436.
[12]刘栋,宋晓红,汤长明,等.HPLC法同时测定软肝缩脾丸中芍药苷和丹酚酸B的含量[J].中国药房,2017,28(3):416-418.